Generic Nitric Oxide (NO) Generating Surface by Immobilizing Organoselenium Species via Layer-by-Layer Assembly
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A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to NO in the presence of thiol reducing agents (e.g., glutathione, cysteine, etc.). The stepwise buildup of the multilayer films is monitored by UV-vis spectroscopy, SEM and surface contact angle measurements. X-ray photoelectron spectroscopy is used to study the stoichiometry between the polyanion and polycation, and also the presence of Se in the catalytic LbL film. A reductive annealing process is necessary to improve the stability of freshly coated multilayer films via chain rearrangement. Chemiluminescence measurements illustrate the ability of the LbL films to generate NO from S-nitrosoglutathione (GSNO) in the presence of glutathione (GSH). Enhanced NO fluxes can be achieved by increasing the number of catalytic (SePEI/Alg) bilayers coated on the substrates. Nitric oxide generation is observed even after prolonged contact with sheep whole blood. Preliminary applications of this LbL on silicone rubber tubings and polyurethane catheters reveal similar NO generation behavior from these biomedical grade polymeric substrates.
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Metal ions such as copper or ferrous ions, or organoselenium compounds, can catalyze RSNO decomposition.
“Metal ions (such as copper or ferrous ions)169,170 or organoselenium compounds can catalyze RSNO decomposition.114,171–173”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [172]
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- Authors
- Yang, J., Welby, J. L., & Meyerhoff, M. E.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Meyerhoff, Mark E.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-08-19
- Volume
- 24
- Issue
- 18
- Pages
- 10265-10272
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- PMC
- PMC2824255
- OpenAlex
- W2001286694
- Cited by
- 55
- References
- 71
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 9 · 2017: 4 · 2016: 5 · 2015: 7 · 2014: 2 · 2013: 4 · 2012: 2
- FWCI
- 1.02
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Langmuir (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (EB 004527, R01EB004527)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- nitric oxide production, layer-by-layer assembly, organoselenium compounds, polyethyleneimine, sodium alginate, S-nitrosoglutathione, glutathione, reductive annealing, multilayer films, hemocompatibility, silicone rubber catheters, polyurethane catheters, nitric oxide flux
- MeSH terms
- Alginates, Catalysis, Hexuronic Acids, Lipid Bilayers, Microscopy, Electron, Scanning, Models, Chemical, Nitric Oxide, Nitrogen, Polymers, Polyurethanes, Quartz, Selenium, Spectrophotometry, Ultraviolet, Sulfhydryl Compounds, Surface Properties, Fluorescein-5-isothiocyanate, Glucuronic Acid
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