Cited by 55Polymer Surface Interaction StudiesNitric Oxide and Endothelin EffectsTissue Engineering and Regenerative Medicine

Generic Nitric Oxide (NO) Generating Surface by Immobilizing Organoselenium Species via Layer-by-Layer Assembly

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Yang, Jun, Welby, Jenna L., Meyerhoff, Mark E.·2008·Langmuir

Abstract

A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to NO in the presence of thiol reducing agents (e.g., glutathione, cysteine, etc.). The stepwise buildup of the multilayer films is monitored by UV-vis spectroscopy, SEM and surface contact angle measurements. X-ray photoelectron spectroscopy is used to study the stoichiometry between the polyanion and polycation, and also the presence of Se in the catalytic LbL film. A reductive annealing process is necessary to improve the stability of freshly coated multilayer films via chain rearrangement. Chemiluminescence measurements illustrate the ability of the LbL films to generate NO from S-nitrosoglutathione (GSNO) in the presence of glutathione (GSH). Enhanced NO fluxes can be achieved by increasing the number of catalytic (SePEI/Alg) bilayers coated on the substrates. Nitric oxide generation is observed even after prolonged contact with sheep whole blood. Preliminary applications of this LbL on silicone rubber tubings and polyurethane catheters reveal similar NO generation behavior from these biomedical grade polymeric substrates.

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Authors
Yang, J., Welby, J. L., & Meyerhoff, M. E.
Affiliations
University of Michigan (US)
Corresponding author
Meyerhoff, Mark E.
Venue
Langmuir
Published
2008-08-19
Volume
24
Issue
18
Pages
10265-10272
ISSN
  • 0743-7463 (ISSN-L)
  • 1520-5827
OpenAlex
W2001286694
Cited by
55
References
71
Citations by year
2026: 2 · 2025: 1 · 2024: 4 · 2023: 2 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 9 · 2017: 4 · 2016: 5 · 2015: 7 · 2014: 2 · 2013: 4 · 2012: 2
FWCI
1.02
Type
journal article
Language
en
Open access
Locations
  • Langmuir (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of Biomedical Imaging and Bioengineering (EB 004527, R01EB004527)
Topics
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
nitric oxide production, layer-by-layer assembly, organoselenium compounds, polyethyleneimine, sodium alginate, S-nitrosoglutathione, glutathione, reductive annealing, multilayer films, hemocompatibility, silicone rubber catheters, polyurethane catheters, nitric oxide flux
MeSH terms
Alginates, Catalysis, Hexuronic Acids, Lipid Bilayers, Microscopy, Electron, Scanning, Models, Chemical, Nitric Oxide, Nitrogen, Polymers, Polyurethanes, Quartz, Selenium, Spectrophotometry, Ultraviolet, Sulfhydryl Compounds, Surface Properties, Fluorescein-5-isothiocyanate, Glucuronic Acid

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