Human iPS derived progenitors bioengineered into liver organoids using an inverted colloidal crystal poly (ethylene glycol) scaffold
Open accessAbstract
Generation of human organoids from induced pluripotent stem cells (iPSCs) offers exciting possibilities for developmental biology, disease modelling and cell therapy. Significant advances towards those goals have been hampered by dependence on animal derived matrices (e.g. Matrigel), immortalized cell lines and resultant structures that are difficult to control or scale. To address these challenges, we aimed to develop a fully defined liver organoid platform using inverted colloid crystal (ICC) whose 3-dimensional mechanical properties could be engineered to recapitulate the extracellular niche sensed by hepatic progenitors during human development. iPSC derived hepatic progenitors (IH) formed organoids most optimally in ICC scaffolds constructed with 140 μm diameter pores coated with type I collagen in a two-step process mimicking liver bud formation. The resultant organoids were closer to adult tissue, compared to 2D and 3D controls, with respect to morphology, gene expression, protein secretion, drug metabolism and viral infection and could integrate, vascularise and function following implantation into livers of immune-deficient mice. Preliminary interrogation of the underpinning mechanisms highlighted the importance of TGFβ and hedgehog signalling pathways. The combination of functional relevance with tuneable mechanical properties leads us to propose this bioengineered platform to be ideally suited for a range of future mechanistic and clinical organoid related applications.
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Cell-binding cues from collagen, fibronectin, or laminin have frequently been added to synthetic hydrogels to allow for organoid growth and differentiation.
“Cell-binding cues from collagen, fibronectin, or laminin have frequently been added to synthetic hydrogels to allow for organoid growth and differentiation.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [158]
Attachment of human iPSCs was successful in assemblies functionalized with collagen I and laminin-521 but not with fibronectin.
“Attachment of human iPSCs was successful in assemblies functionalized with collagen I and laminin-521 but not with fibronectin.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [158]
Ng and colleagues created functional human liver organoids derived from human iPSCs in a colloidal crystal of PEG functionalized with collagen I, fibronectin, or laminin-521.
“Ng and colleagues created functional human liver organoids derived from human iPSCs in a colloidal crystal of PEG functionalized with collagen I, fibronectin, or laminin-521158,159.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [158]
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Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204 · see citing works
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372 · see citing works
Organoid Models and Applications in Biomedical Research
Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · The Nephron journals/Nephron journals · doi:10.1159/000433566 · Cited by 3,517 · see citing works
Vascularized and functional human liver from an iPSC-derived organ bud transplant
Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170 · see citing works
Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis
Takasato, M., Er, P. X., Chiu, H. S., Maier, B., Baillie, G. J., Ferguson, C., Parton, R. G., Wolvetang, E. J., Roost, M. S., Chuva de Sousa Lopes, S. M., et al. (2015) · Nature · doi:10.1038/nature15695 · Cited by 1,627 · see citing works
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
Huch, M., Dorrell, C., Boj, S. F., van Es, J. H., Li, V. S. W., van de Wetering, M., Sato, T., Hamer, K., Sasaki, N., Finegold, M. J., et al. (2013) · Nature · doi:10.1038/nature11826 · Cited by 1,555 · see citing works
Organoids as an in vitro model of human development and disease
Fatehullah, A., Tan, S. H., & Barker, N. (2016) · Nature Cell Biology · doi:10.1038/ncb3312 · Cited by 1,508 · see citing works
Designer matrices for intestinal stem cell and organoid culture
Gjorevski, N., Sachs, N., Manfrin, A., Giger, S., Bragina, M. E., Ordóñez-Morán, P., Clevers, H., & Lutolf, M. P. (2016) · Nature · doi:10.1038/nature20168 · Cited by 1,426 · see citing works
Bell, C. C., Hendriks, D. F. G., Moro, S. M. L., Ellis, E., Walsh, J., Renblom, A., Fredriksson Puigvert, L., Dankers, A. C. A., Jacobs, F., Snoeys, J., et al. (2016) · Scientific Reports · doi:10.1038/srep25187 · Cited by 739 · see citing works
Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells
Rashid, S. T., Corbineau, S., Hannan, N. R. F., Marciniak, S. J., Miranda, E., Alexander, G., Huang‐Doran, I., Griffin, J. L., Ährlund‐Richter, L., Skepper, J. N., et al. (2010) · Journal of Clinical Investigation · doi:10.1172/jci43122 · Cited by 598 · see citing works
Self-citationMetabolic zonation of the liver: The oxygen gradient revisited
Kietzmann, T. (2017) · Redox Biology · doi:10.1016/j.redox.2017.01.012 · Cited by 499 · see citing works
Orchestrating liver development
Gordillo, M., Evans, T., & Gouon-Evans, V. (2015) · Development · doi:10.1242/dev.114215 · Cited by 375 · cited 3× · see citing works
“3D), allowing us to conclude that organoids became established with unique cellular arrangement in a structure reminiscent of the hepatic buds seen in embryonic [15] and regenerating human liver tissue [16].”
BackgroundMethodInfluentialProduction of hepatocyte-like cells from human pluripotent stem cells
Hannan, N. R. F., Segeritz, C., Touboul, T., & Vallier, L. (2013) · Nature Protocols · doi:10.1038/nprot.2012.153 · Cited by 327 · see citing works
Human Hepatic Stem Cell and Maturational Liver Lineage Biology Δ
Turner, R., Lozoya, O. A., Wang, Y., Cardinale, V., Gaudio, E., Alpini, G. D., Mendel, G., Wauthier, E., Barbier, C., Alvaro, D., et al. (2011) · Hepatology · doi:10.1002/hep.24157 · Cited by 316 · see citing works
Baghbaderani, B. A., Tian, X., Neo, B. H., Burkall, A., Dimezzo, T., Sierra, G., Zeng, X., Warren, K., Kovarcik, D. P., Fellner, T., et al. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.08.015 · Cited by 212 · see citing works
Linking hypoxia, DNA damage and proliferation in multicellular tumor spheroids
Riffle, S., Pandey, R. N., Albert, M., & Hegde, R. S. (2017) · BMC Cancer · doi:10.1186/s12885-017-3319-0 · Cited by 142 · see citing works
Polarization Restricts Hepatitis C Virus Entry into HepG2 Hepatoma Cells
Mee, C., Harris, H. J., Farquhar, M. J., Wilson, G. K., Reynolds, G. M., Davis, C., van IJzendoorn, S. C. D., Balfe, P., & McKeating, J. A. (2009) · Journal of Virology · doi:10.1128/jvi.00246-09 · Cited by 137 · see citing works
Characterization of a functional C3A liver spheroid model
Gaskell, H., Sharma, P., Colley, H. E., Murdoch, C., Williams, D. P., & Webb, S. D. (2016) · Toxicology Research · doi:10.1039/c6tx00101g · Cited by 131 · see citing works
Lee, J., Acosta, E. G., Stoeck, I. K., Long, G., Hiet, M., Mueller, B., Fackler, O., Kallis, S., & Bartenschlager, R. F. W. (2014) · Journal of Virology · doi:10.1128/jvi.01660-14 · Cited by 126 · see citing works
Stueck, A., & Wanless, I. R. (2015) · Hepatology · doi:10.1002/hep.27706 · Cited by 109 · see citing works
Langan, L. M., Dodd, N. J. F., Owen, S. F., Purcell, W. M., Jackson, S. K., & Jha, A. N. (2016) · PLOS ONE · doi:10.1371/journal.pone.0149492 · Cited by 101 · see citing works
Molina‐Jiménez, F., Benedicto, I., Thi, V. L. D., Gondar, V., Lavillette, D., Marı́n, J. J. G., Briz, Ó., Moreno‐Otero, R., Aldabe, R., Baumert, T. F., et al. (2012) · Virology · doi:10.1016/j.virol.2011.12.021 · Cited by 90 · see citing works
Gramignoli, R., Green, M. L., Tahan, V., Dorko, K., Skvorak, K. J., Marongiu, F., Zao, W., Venkataramanan, R., Ellis, E. C. S., Geller, D. A., et al. (2011) · Cell Transplantation · doi:10.3727/096368911x600939 · Cited by 73 · see citing works
Proteomics of HCV virions reveals an essential role for the nucleoporin Nup98 in virus morphogenesis
Lussignol, M., Kopp, M., Molloy, K. R., Vizcay‐Barrena, G., Fleck, R. A., Dorner, M., Bell, K. L., Chait, B. T., Rice, C. M., & Catanese, M. T. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1518934113 · Cited by 67 · see citing works
Wang, Y., Kim, M. H., Shirahama, H., Lee, J. H., Ng, S. S., Glenn, J. S., & Cho, N. (2016) · Scientific Reports · doi:10.1038/srep37427 · Cited by 42 · see citing works
Self-citationLong-term culture of human liver tissue with advanced hepatic functions
Ng, S. S., Xiong, A., Nguyen, K., Masek, M., No, D. Y., Elazar, M., Shteyer, E., Winters, M. A., Voedisch, A., Shaw, K., et al. (2017) · JCI Insight · doi:10.1172/jci.insight.90853 · Cited by 32 · see citing works
Self-citationBiofunctionalized Hydrogel Microscaffolds Promote 3D Hepatic Sheet Morphology
Kim, M. H., Kumar, S. K., Shirahama, H., Seo, J., Lee, J. H., Zhdanov, V. P., & Cho, N. (2015) · Macromolecular Bioscience · doi:10.1002/mabi.201500338 · Cited by 21 · see citing works
Self-citationPhenotypic regulation of liver cells in a biofunctionalized three-dimensional hydrogel platform
Kim, M. H., Kumar, S. K., Shirahama, H., Seo, J., Lee, J. H., & Cho, N. (2016) · Integrative Biology · doi:10.1039/c5ib00269a · Cited by 19 · see citing works
Self-citationHua, M., Zhang, W., Li, W., Li, X., Liu, B., Lü, X., & Zhang, H. (2012) · Journal of Cell Science · doi:10.1242/jcs.110551 · Cited by 15 · see citing works
Shirahama, H., Kumar, S. K., Jeon, W.-Y., Kim, M. H., Lee, J. H., Ng, S. S., Tabaei, S. R., & Cho, N.-J. (2016) · Journal of Visualized Experiments · doi:10.3791/54331 · Cited by 14 · see citing works
Self-citationShirahama, H., Kumar, S. K., Jeon, W., Kim, M. H., Lee, J. H., Ng, S. S., Tabaei, S. R., & Cho, N. (2016) · Journal of Visualized Experiments · doi:10.3791/54331-v · Cited by 6 · see citing works
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LeSavage, B. L., Suhar, R. A., Broguière, N., Lütolf, M. P., & Heilshorn, S. C. (2021) · Nature Materials · doi:10.1038/s41563-021-01057-5 · Cited by 462 · see citing works
Towards organoid culture without Matrigel
Kozlowski, M. T., Crook, C. J., & Ku, H. T. (2021) · Communications Biology · doi:10.1038/s42003-021-02910-8 · Cited by 409 · see citing works
Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip
Liu, H., Wang, Y., Cui, K., Guo, Y., Zhang, X., & Qin, J. (2019) · Advanced Materials · doi:10.1002/adma.201902042 · Cited by 353 · see citing works
Bioengineering Approaches for the Advanced Organoid Research
Yi, S. A., Zhang, Y., Rathnam, C., Pongkulapa, T., & Lee, K. (2021) · Advanced Materials · doi:10.1002/adma.202007949 · Cited by 218 · see citing works
AI-enabled organoids: Construction, analysis, and application
Bai, L., Wu, Y., Li, G., Zhang, W., Zhang, H., & Su, J. (2023) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.09.005 · Cited by 216 · see citing works
A Droplet Microfluidic System to Fabricate Hybrid Capsules Enabling Stem Cell Organoid Engineering
Liu, H., Wang, Y., Wang, H., Zhao, M., Tao, T., Zhang, X., & Qin, J. (2020) · Advanced Science · doi:10.1002/advs.201903739 · Cited by 191 · see citing works
Cell therapy for advanced liver diseases: repair or rebuild
Dwyer, B. J., Macmillan, M. T., Brennan, P. N., & Forbes, S. J. (2021) · Journal of Hepatology · doi:10.1016/j.jhep.2020.09.014 · Cited by 141 · see citing works
iPSC-Derived Hepatocytes as a Platform for Disease Modeling and Drug Discovery
Corbett, J., & Duncan, S. A. (2019) · Frontiers in Medicine · doi:10.3389/fmed.2019.00265 · Cited by 139 · see citing works
Recent advances in defined hydrogels in organoid research
Gan, Z., Qin, X., Liu, H., Liu, J., & Qin, J. (2023) · Bioactive Materials · doi:10.1016/j.bioactmat.2023.06.004 · Cited by 127 · see citing works
Zhang, L., Ma, X.-J.-N., Fei, Y.-Y., Han, H., Xu, J., Cheng, L., & Li, X. (2021) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2021.108004 · Cited by 93 · see citing works
Synthetic scaffolds for 3D cell cultures and organoids: applications in regenerative medicine
Marchini, A., & Gelain, F. (2022) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1932716 · Cited by 91 · see citing works
Liver Tissue Engineering: Challenges and Opportunities
Agarwal, T., Subramanian, B., & Maiti, T. K. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00745 · Cited by 83 · see citing works
One-step synthesis of composite hydrogel capsules to support liver organoid generation from hiPSCs
Wang, Y., Liu, H., Zhang, M., Wang, H., Chen, W., & Qin, J. (2020) · Biomaterials Science · doi:10.1039/d0bm01085e · Cited by 78 · see citing works
Recent methods of droplet microfluidics and their applications in spheroids and organoids
Wang, Y., Liu, M., Zhang, Y., Liu, H., & Han, L. G. (2023) · Lab on a Chip · doi:10.1039/d2lc00493c · Cited by 83 · see citing works
Biomedical Application of Functional Materials in Organ-on-a-Chip
Ding, C., Chen, X., Kang, Q., & Yan, X. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00823 · Cited by 77 · see citing works
Chang, P.-H., Chao, H., Chern, E., & Hsu, S. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120575 · Cited by 68 · see citing works
Prasad, M., Kumar, R., Buragohain, L., Kumari, A., & Ghosh, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.696668 · Cited by 62 · see citing works
Blackford, S. J. I., Ng, S. S., Segal, J. M., King, A., Austin, A. L. F., Kent, D., Moore, J. C., Sheldon, M., Ilić, D., Dhawan, A. K., et al. (2018) · Stem Cells Translational Medicine · doi:10.1002/sctm.18-0084 · Cited by 61 · see citing works
Self-citationThe frontier of live tissue imaging across space and time
Huang, Q., Garrett, A., Bose, S., Blocker, S. J., Rios, A. C., Clevers, H. C., & Shen, X. (2021) · Cell stem cell · doi:10.1016/j.stem.2021.02.010 · Cited by 59 · see citing works
Goulart, E., de Caires-Junior, L. C., Telles-Silva, K. A., Araújo, B. H. S., Kobayashi, G. S., Musso, C. M., Assoni, A. F., Oliveira, D., Caldini, E. T. E. G., Gerstenhaber, J. A., et al. (2019) · Stem Cell Research & Therapy · doi:10.1186/s13287-019-1367-x · Cited by 57 · see citing works
Morais, A. D. S., Vieira, S., Zhao, X., Mao, Z., Gao, C., Oliveira, J. M., & Reis, R. L. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.201901435 · Cited by 56 · see citing works
Advanced lung organoids for respiratory system and pulmonary disease modeling
Joo, H., Min, S., & Cho, S. (2024) · Journal of Tissue Engineering · doi:10.1177/20417314241232502 · Cited by 60 · see citing works
Li, C., An, N., Song, Q., Hu, Y., Yin, W., Wang, Q., Le, Y., Pan, W., Yan, X., Wang, Y., et al. (2024) · Journal of Biomedical Science · doi:10.1186/s12929-024-01086-7 · Cited by 57 · see citing works
Yap, K. K., Gerrand, Y.-W., Dingle, A. M., Yeoh, G. C., Morrison, W. A., & Mitchell, G. M. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120091 · Cited by 54 · see citing works
Zhang, J., Chan, H. F., Wang, H., Shao, D., Tao, Y., & Li, M. (2021) · Journal of Tissue Engineering · doi:10.1177/2041731420986711 · Cited by 54 · see citing works
Advancing Organoid Engineering for Tissue Regeneration and Biofunctional Reconstruction
Jin, H., Xue, Z., Liu, J., Ma, B., Yang, J., & Lei, L. (2024) · Biomaterials Research · doi:10.34133/bmr.0016 · Cited by 53 · see citing works
Adigbli, G., Ménoret, S., Cross, A. R., Hester, J., Issa, F., & Anegón, I. (2020) · Transplantation · doi:10.1097/tp.0000000000003177 · Cited by 51 · see citing works
Organoids and regenerative hepatology
Jalan‐Sakrikar, N., Brevini, T., Huebert, R. C., & Sampaziotis, F. (2022) · Hepatology · doi:10.1002/hep.32583 · Cited by 50 · see citing works
Vascularization of engineered organoids
Liu, H., Zhang, X., Liu, J., & Qin, J. (2023) · BMEMat · doi:10.1002/bmm2.12031 · Cited by 46 · see citing works
Jiang, S., Xu, F., Jin, M., Wang, Z., Xu, X., Zhou, Y., Wang, J., Gu, L., Han, F., Fan, Y., et al. (2022) · Biofabrication · doi:10.1088/1758-5090/ac933c · Cited by 46 · see citing works
Kasturi, M., Mathur, V., Gadre, M. A., Srinivasan, V., & Vasanthan, K. S. (2023) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-023-00576-3 · Cited by 44 · see citing works
Human liver model systems in a dish
Thompson, W. L., & Takebe, T. (2021) · Development Growth & Differentiation · doi:10.1111/dgd.12708 · Cited by 49 · see citing works
Advancements in stem cell-derived hepatocyte-like cell models for hepatotoxicity testing
Jin, M., Xiao, Y., Liao, W., Chen, Q., Yang, W., Li, Y., Li, S., Gao, Y., Peng, Q., & Zhou, S. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02152-9 · Cited by 41 · see citing works
<i>In vitro</i> functional models for human liver diseases and drug screening: beyond animal testing
Paradiso, A., Volpi, M., Rinoldi, C., Celikkin, N., Contessi Negrini, N., Bilgen, M., Dallera, G., Pierini, F., Costantini, M., Święszkowski, W., et al. (2023) · Biomaterials Science · doi:10.1039/d1bm01872h · Cited by 40 · see citing works
Michielin, F., Giobbe, G. G., Luni, C., Hu, Q., Maroni, I., Orford, M., Manfredi, A., Filippo, L. D., David, A. L., Cacchiarelli, D., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108453 · Cited by 41 · see citing works
Ma, P., Chen, Y., Lai, X., Zheng, J., Ye, E., Loh, X. J., Zhao, Y., Parikh, B. H., Su, X., You, M., et al. (2021) · Macromolecular Bioscience · doi:10.1002/mabi.202100191 · Cited by 39 · see citing works
Liver organoids: established tools for disease modeling and drug development
Sun, X.-C., Kong, D.-F., Zhao, J., Faber, K. N., Xia, Q., & He, K. (2023) · Hepatology Communications · doi:10.1097/hc9.0000000000000105 · Cited by 37 · see citing works
Biomimetic niches reveal the minimal cues to trigger apical lumen formation in single hepatocytes
Zhang, Y., De Mets, R., Monzel, C., Acharya, V., Toh, P., Chin, J. F. L., Van Hul, N., Ng, I. C., Yu, H., Ng, S. S., et al. (2020) · Nature Materials · doi:10.1038/s41563-020-0662-3 · Cited by 37 · see citing works
Self-citationKim, D., Youn, J., Kim, J., Lee, J., Yoon, J., & Kim, D. S. (2026) · npj Biomedical Innovations. · doi:10.1038/s44385-025-00054-6 · Cited by 39 · see citing works
Engineering Natural Pollen Grains as Multifunctional 3D Printing Materials
Chen, S., Shi, Q., Jang, T., Ibrahim, M. S., Deng, J., Ferracci, G., Tan, W. S., Cho, N., & Song, J. (2021) · Advanced Functional Materials · doi:10.1002/adfm.202106276 · Cited by 35 · see citing works
Self-citationGelatin-Based Hybrid Hydrogels as Matrices for Organoid Culture
Carpentier, N., Ye, S., Delemarre, M. D., der Meeren, L. V., Skirtach, A. G., van der Laan, L. J. W., Schneeberger, K., Spee, B., Dubruel, P., & Vlierberghe, S. V. (2024) · Biomacromolecules · doi:10.1021/acs.biomac.2c01496 · Cited by 34 · see citing works
Tricot, T., Verfaillie, C. M., & Kumar, M. (2022) · Cells · doi:10.3390/cells11030442 · Cited by 35 · see citing works
Generation of human liver organoids from pluripotent stem cell-derived hepatic endoderms
Kulkeaw, K., Tubsuwan, A., Tongkrajang, N., & Whangviboonkij, N. (2020) · PeerJ · doi:10.7717/peerj.9968 · Cited by 32 · see citing works
Challenges for the Applications of Human Pluripotent Stem Cell-Derived Liver Organoids
Chang, M., Bogacheva, M. S., & Lou, Y. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.748576 · Cited by 32 · see citing works
Ogoke, O., Maloy, M. H., & Parashurama, N. (2020) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/brv.12650 · Cited by 28 · see citing works
Stem Cells and Hydrogels for Liver Tissue Engineering: Synergistic Cure for Liver Regeneration
Nadi, A., Moradi, L., Ai, J., & Asadpour, S. (2020) · Stem Cell Reviews and Reports · doi:10.1007/s12015-020-10060-3 · Cited by 29 · see citing works
Yang, J., Zhang, X., Liu, Z., Yang, C., Li, S., Zhou, H., & Gao, Z. (2024) · Chemical Engineering Journal · doi:10.1016/j.cej.2024.155882 · Cited by 28 · see citing works
Synthetic developmental biology: Engineering approaches to guide multicellular organization
Zarkesh, I., Ashtiani, M. K., Shiri, Z., Aran, S., Braun, T. W., & Baharvand, H. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.02.004 · Cited by 27 · see citing works
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Wang, Y., Keshavarz, M., Barhouse, P. S., & Smith, Q. (2022) · Advanced Biology · doi:10.1002/adbi.202200050 · Cited by 27 · see citing works
Blackford, S. J. I., Yu, T. T. L., Norman, M. D. A., Syanda, A. M., Manolakakis, M., Lachowski, D., Yan, Z., Guo, Y., Garitta, E., Riccio, F., et al. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121982 · Cited by 28 · see citing works
Self-citationAbbasalizadeh, S., Babaee, S., Kowsari‐Esfahan, R., Mazidi, Z., Shi, Y., Wainer, J. A., Cabral, J. M. S., Langer, R., Traverso, G., & Baharvand, H. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202210233 · Cited by 27 · see citing works
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Qian, S., Mao, J., Liu, Z., Zhao, B., Zhao, Q., Lu, B., Zhang, L., Mao, X., Cheng, L., Cui, W., et al. (2022) · Smart Medicine · doi:10.1002/smmd.20220007 · Cited by 25 · see citing works
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Septiana, W. L., & Pawitan, J. A. (2024) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-024-00672-y · Cited by 26 · see citing works
Lee, J., Park, D., Seo, Y., Chung, J. J., Jung, Y., & Kim, S. H. (2020) · Advanced Materials · doi:10.1002/adma.202002096 · Cited by 24 · see citing works
Zhao, K.-Y., Du, Y., Cao, H., Su, L., Su, X., & Li, X. (2024) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2024.114435 · Cited by 24 · see citing works
Farshidfar, N., Assar, S., Amiri, M. A., Sahmeddini, S., Hamedani, S., Zarei, M., & Tayebi, L. (2023) · Bio-Design and Manufacturing · doi:10.1007/s42242-023-00235-5 · Cited by 23 · see citing works
Li, Y., Yang, X., Plummer, R., Hayashi, Y., Deng, X.-S., Nie, Y., & Taniguchi, H. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms221910471 · Cited by 24 · see citing works
Ong, J., Zhao, J., Levy, G. K., Macdonald, J., Justin, A. W., & Markaki, A. E. (2020) · Scientific Reports · doi:10.1038/s41598-020-69301-7 · Cited by 21 · see citing works
Roudaut, M., Caillaud, A., Souguir, Z., Bray, L., Girardeau, A., Rimbert, A., Croyal, M., Lambert, G. C., Patitucci, M., Delpouve, G., et al. (2024) · Bioengineering & Translational Medicine · doi:10.1002/btm2.10659 · Cited by 20 · see citing works
Cao, D., Ge, J.-Y., Wang, Y., Oda, T., & Zheng, Y. (2021) · World Journal of Gastroenterology · doi:10.3748/wjg.v27.i29.4784 · Cited by 19 · see citing works
Advances in liver organoids: model systems for liver disease
Lee, J., Mun, S. J., Shin, Y., Lee, S., & Son, M. J. (2022) · Archives of Pharmacal Research · doi:10.1007/s12272-022-01390-6 · Cited by 21 · see citing works
Modeling human liver organ development and diseases with pluripotent stem cell-derived organoids
Ouchi, R., & Koike, H. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1133534 · Cited by 17 · see citing works
Thermoresponsive Inverted Colloidal Crystal Hydrogel Scaffolds for Lymphoid Tissue Engineering
Kwak, J., & Lee, J. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.201901556 · Cited by 18 · see citing works
Human iPSC-derived hepatocyte system models cholestasis with tight junction protein 2 deficiency
Li, C. Z., Ogawa, H., Ng, S. S., Chen, X., Kishimoto, E., Sakabe, K., Fukami, A., Hu, Y., Mayhew, C. N., Hellmann, J., et al. (2022) · JHEP Reports · doi:10.1016/j.jhepr.2022.100446 · Cited by 17 · see citing works
Self-citationEmerging biotechnologies for engineering liver organoids
Zhao, J., Zhi, Y., Ren, H., Wang, J., & Zhao, Y. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.11.002 · Cited by 17 · see citing works
Matrigel Tunes H9 Stem Cell-Derived Human Cerebral Organoid Development
Estridge, R. C., O’Neill, J. E., & Keung, A. J. (2023) · Organoids · doi:10.3390/organoids2040013 · Cited by 17 · see citing works
Progress, application and challenges of liver organoids
Sang, C., Lin, J., Ji, S., & Gao, Q. (2024) · Clinical Cancer Bulletin · doi:10.1007/s44272-024-00012-0 · Cited by 13 · see citing works
Liu, W., Zhou, X., Chen, C., Lv, D.-D., Huang, W., Peng, Y. Y., Wu, H., Chen, Y., Tang, D., Guo, L., et al. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.738081 · Cited by 13 · see citing works
Wu, Y. C., Wu, G. X., Chen, K., Shiu, L., Kumar, S., Liu, G., & Kuo, S. M. (2022) · Scientific Reports · doi:10.1038/s41598-022-05174-2 · Cited by 13 · see citing works
Application of colloidal photonic crystals in study of organoids
Huang, K., Li, Q., Xue, Y., Wang, Q., Chen, Z., & Gu, Z. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.115075 · Cited by 13 · see citing works
Zhang, L., Wang, H., Yang, R., Liu, M., Ban, Q., Chen, W., Zhao, M., You, R., Jin, Y., & Guan, Y. (2018) · Chemical Engineering Journal · doi:10.1016/j.cej.2018.10.230 · Cited by 13 · see citing works
Liver Buds and Liver Organoids: New Tools for Liver Development, Disease and Medical Application
Zeng, F., Zhang, Y., Han, X., Weng, J., & Gao, Y. (2019) · Stem Cell Reviews and Reports · doi:10.1007/s12015-019-09909-z · Cited by 13 · see citing works
Highly efficient construction of monkey blastoid capsules from aged somatic cells
Wu, J., Shao, T., Tang, Z., Liu, G., Li, Z., Shi, Y., Kang, Y., Zuo, J., Zhao, B., Hu, G., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-56447-z · Cited by 12 · see citing works
Natural Scaffolds Used for Liver Regeneration: A Narrative Update
Vazirzadeh, M., Azarpira, N., Davoodi, P., Vosough, M., & Ghaedi, K. (2022) · Stem Cell Reviews and Reports · doi:10.1007/s12015-022-10362-8 · Cited by 12 · see citing works
Microfluidic Techniques for Next‐Generation Organoid Systems
Gong, J., Li, M., Kang, J., Yin, Z., Cha, Z., Yang, J., & Xu, H. (2022) · Advanced Materials Interfaces · doi:10.1002/admi.202200846 · Cited by 12 · see citing works
Collagen-based biomaterials for tissue engineering applications
Amirthalingam, S., & Hwang, N. S.-Y. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-98827-8.00025-4 · Cited by 11 · see citing works
Liver organoids: From 3D printing to biomedical applications
Shi, Y., Han, X., Zhang, Z., Xu, J., & Liu, G. (2024) · BMEMat · doi:10.1002/bmm2.12129 · Cited by 12 · see citing works
Induced Pluripotent Stem Cells (Ipscs) Based Liver Organoid: the Benefits and Challenges
Septiana, W. L., Noviantari, A., & Antarianto, R. D. (2023) · Cellular Physiology and Biochemistry · doi:10.33594/000000662 · Cited by 10 · see citing works
Advances in 3D cell culture for liver preclinical studies
Yao, T., Zhang, Y., Lv, M., Zang, G., Ng, S. S., & Chen, X. (2021) · Acta Biochimica et Biophysica Sinica · doi:10.1093/abbs/gmab046 · Cited by 10 · see citing works
Self-citationMechanobiological engineering strategies for organoid culture
Taghizadeh, M., Taghizadeh, A. A., & Kim, H. S. (2025) · APL Bioengineering · doi:10.1063/5.0275439 · Cited by 10 · see citing works
Baas, F. S., Rishi, G., Swinkels, D. W., & Subramaniam, V. N. (2021) · Clinical Chemistry · doi:10.1093/clinchem/hvab130 · Cited by 9 · see citing works
Three-Dimensional Cell Culture Systems for Studying Hepatitis C Virus
So, C.-W., & Randall, G. C. (2021) · Viruses · doi:10.3390/v13020211 · Cited by 8 · see citing works
Kuo, Y., Lin, S.-Y., De, S., & Rajesh, R. (2023) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.3c00791 · Cited by 8 · see citing works
Hepatobiliary organoid research: the progress and applications
Zou, R.-Q., Dai, Y.-S., Liu, F., Yang, S., Hu, H., & Li, F. (2025) · Frontiers in Pharmacology · doi:10.3389/fphar.2025.1473863 · Cited by 7 · see citing works
Developments in gastrointestinal organoid cultures to recapitulate tissue environments
Kuhn, M. R., Wolcott, E. A., & Langer, E. M. (2025) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2025.1521044 · Cited by 8 · see citing works
Bioengineering an Artificial Human Blood–Brain Barrier in Rodents
Kamal, K., & Waldau, B. (2019) · Bioengineering · doi:10.3390/bioengineering6020038 · Cited by 7 · see citing works
Research Dynamics of Tissue Spheroids as Building Blocks: A Scientometric Analysis
Rodríguez-Salvador, M., Fox-Miranda, I., Pérez-Benítez, B. E., & López-Robles, J. R. (2022) · International Journal of Bioprinting · doi:10.18063/ijb.v8i3.585 · Cited by 7 · see citing works
3D Hydrogel Cell Cultures and Their Biomedical Applications
Thai, T. L., Manchanda, A., Higginbottom, S. L., Rodriguez, E. S., Wilson, R., Tomaskovic‐Crook, E., Crook, J. M., & Paull, B. (2025) · Advanced NanoBiomed Research · doi:10.1002/anbr.202500133 · Cited by 6 · see citing works
Chen, J., Shen, L., Guo, Q., Ma, S., Zhang, Y., Chen, J., Qu, L., Ng, S. S., & Chen, X. (2024) · Journal of Medical Virology · doi:10.1002/jmv.29546 · Cited by 6 · see citing works
Self-citationTakezawa, T., & Uzu, M. (2022) · The Journal of Toxicological Sciences · doi:10.2131/jts.47.39 · Cited by 6 · see citing works
The native liver as inspiration to create superior in vitro hepatic models
Carpentier, N., Urbani, L., Dubruel, P., & Vlierberghe, S. V. (2023) · Biomaterials Science · doi:10.1039/d2bm01646j · Cited by 6 · see citing works
Fabrication of Bioactive Inverted Colloidal Crystal Scaffolds Using Expanded Polystyrene Beads
Carpenter, R., Macres, D., Kwak, J., Daniel, K., & Lee, J. (2020) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2019.0333 · Cited by 6 · see citing works
Duan, K., Hu, J., Zhao, Y., Lin, M., Xu, M., Li, T., & Lee, B. H. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.146376 · Cited by 5 · see citing works
Cell and cell-derivative-based therapy for liver diseases: current approaches and future promises
Zahmatkesh, E., Rad, N. K., Hossein‐Khannazer, N., Mohamadnejad, M., Gramignoli, R., Najimi, M., Malekzadeh, R., Hassan, M., & Vosough, M. (2023) · Expert Review of Gastroenterology & Hepatology · doi:10.1080/17474124.2023.2172398 · Cited by 5 · see citing works
iPSCs for modeling hepatotropic pathogen infections
Bove, G., Mehnert, A.-K., & Thi, V. L. D. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-823808-0.00013-4 · Cited by 5 · see citing works
Craft, K., Amanor, A., Barnett, I., Donaldson, C., Anegón, I., Madduri, S., Tang, Q., & Bility, M. T. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26073082 · Cited by 4 · see citing works
Recapitulating the liver niche in vitro
Yap, K. K., & Mitchell, G. M. (2022) · Advances in stem cells and their niches · doi:10.1016/bs.asn.2021.10.002 · Cited by 3 · see citing works
Wu, J.-W., Liu, T., Zhang, X.-T., Qu, C., Wu, J., Xu, S.-L., Yang, J., & Xing, X.-Q. (2025) · Biofabrication · doi:10.1088/1758-5090/adde15 · Cited by 2 · see citing works
- Authors
- Ng, S. S., Saeb-Parsy, K., Blackford, S. J. I., Segal, J. M., Serra, M. P., Horcas-Lopez, M., No, D. Y., Mastoridis, S., Jassem, W., Frank, C. W., Cho, N. J., Nakauchi, H., Glenn, J. S., & Rashid, S. T.
- Affiliations
- King's College London (GB)
- Stanford Medicine (US)
- Stanford University (US)
- Nanyang Technological University (SG)
- Corresponding author
- Glenn, Jeffrey S.
- Rashid, S. Tamir
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2018-11
- Volume
- 182
- Pages
- 299-311
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC6131727
- OpenAlex
- W2883652711
- Cited by
- 133
- References
- 31
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 14 · 2023: 19 · 2022: 18 · 2021: 26 · 2020: 23 · 2019: 9 · 2018: 3
- FWCI
- 15.85
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Biomaterials (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research Portal (King's College London) (submittedVersion, cc-by)
- Apollo (University of Cambridge) (submittedVersion, cc-by)
- DR-NTU (Nanyang Technological University) (submittedVersion, cc-by)
- Apollo (cc-by)
- Funders
- National Institute for Health Research (NIHR), United Kingdom
- Guy's and St Thomas' NHS Foundation Trust, United Kingdom
- NIHR Biomedical Research, United Kingdom
- Medical Research Council (MRC) (MGSBACR), United Kingdom
- Alpha-1 Antitrypsin Foundation, United Kingdom
- National Institutes of Health (R01AI099245, U19AI109662)
- Burroughs Wellcome Fund
- California Institute for Regenerative Medicine (LA1_C12-06917)
- National Research Foundation of Singapore (NRF-CRP10-2012-07)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- liver organoids, induced pluripotent stem cells, polyethylene glycol, type I collagen coating, scaffold mechanical properties, TGF-beta signaling, Hedgehog signaling, drug metabolism, disease modeling
- MeSH terms
- Biocompatible Materials, Cells, Cultured, Crystallization, Humans, Liver, Organoids, Polyethylene Glycols, Tissue Engineering, Tissue Scaffolds, Induced Pluripotent Stem Cells
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