In situ differentiation and generation of functional liver organoids from human iPSCs in a 3D perfusable chip system
Abstract
We present a new strategy to engineer human iPSC-derived liver organoids by combining stem cell biology with a microfluidic chip system.
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Droplet-based microfluidics enabled better scRNA-seq analysis of intestinal organoid cell identities during developmental stages, revealing extensive population heterogeneity.
“authors use droplet-based microfluidics to perform better scRNA-seq analysis of intestinal organoid cell identities during various developmental stages, revealing extensive population heterogeneity76.”
From Organoids (2022) · cites this paper as [76]
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- Authors
- Wang, Y., Wang, H., Deng, P., Chen, W., Guo, Y., Tao, T., & Qin, J.
- Affiliations
- Dalian Institute of Chemical Physics (CN)
- Corresponding author
- Qin, Jianhua
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2018
- Volume
- 18
- Issue
- 23
- Pages
- 3606-3616
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2896011625
- Cited by
- 222
- References
- 39
- Citations by year
- 2026: 22 · 2025: 28 · 2024: 35 · 2023: 27 · 2022: 36 · 2021: 34 · 2020: 24 · 2019: 15 · 2014: 1
- FWCI
- 7.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lab on a Chip (publishedVersion)
- PubMed (publishedVersion)
- Dalian Institute of Chemical Physics, CAS Institutional Repository (submittedVersion)
- Funders
- Chinese Academy of Sciences (XDA16020900, XDPB0305)
- National Natural Science Foundation of China (91543121, 31671038, 81573394, 31600784)
- Dalian Institute of Chemical Physics (DICP TMSR201601)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- liver organoids, human induced pluripotent stem cells, embryoid bodies, hepatocyte differentiation, cholangiocyte differentiation, acetaminophen hepatotoxicity, drug testing, liver disease modeling, regenerative medicine
- MeSH terms
- Cell Differentiation, Cell Survival, Equipment Design, Humans, Liver, Organoids, Perfusion, Tissue Array Analysis, Hepatocyte Nuclear Factor 3-beta, SOXF Transcription Factors, Induced Pluripotent Stem Cells
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