In vitro functional models for human liver diseases and drug screening: beyond animal testing

Paradiso, Alessia; Volpi, Marina; Rinoldi, Chiara; Celikkin, Nehar; Contessi Negrini, Nicola; Bilgen, Muge; Dallera, Giorgio; Pierini, Filippo; Costantini, Marco; Święszkowski, Wojciech; Farè, Silvia

doi:10.1039/d1bm01872h

Cited by 403D Printing in Biomedical ResearchTissue Engineering and Regenerative MedicineLiver physiology and pathology

In vitro functional models for human liver diseases and drug screening: beyond animal testing

Open access

Paradiso, Alessia, Volpi, Marina, Rinoldi, Chiara, Celikkin, Nehar, Contessi Negrini, Nicola, Bilgen, Muge, Dallera, Giorgio, Pierini, Filippo +3 more·2023·Biomaterials Science

DOI 10.1039/d1bm01872h

Open this paper in CoChat — free account Open PDF

Abstract

models have been proposed as an alternative to pre-clinical animal models. These systems have been successfully employed for the investigation of the etiology and dynamics of liver diseases, for drug screening, and - more recently - to design patient-tailored therapies, resulting in potentially higher efficacy and reduced costs compared to other methods. Here, we review the most recent advances in this rapidly evolving field with particular attention to organoid cultures, liver-on-a-chip platforms, and engineered scaffold-based approaches.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1039/d1bm01872h

Cites

230 of 230 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Physiological relevant in vitro models provide a promising platform for modelling the physiochemical conditions of the hepatic microenvironment.
“Physiological relevant in vitro models provide a promising platform for modelling the physiochemical conditions of the hepatic microenvironment (Janani and Mandal, 2021;Kostrzewski et al., 2021;Paradiso et al., 2023).”
From Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chip (2024) · cites this paper as Paradiso et al., 2023

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915 · see citing works
Re-epithelialization and immune cell behaviour in an ex vivo human skin model
Rakita, A., Nikolić, N., Mildner, M., Matiasek, J., & Elbe‐Bürger, A. (2020) · Scientific Reports · doi:10.1038/s41598-019-56847-4 · Cited by 8,228 · see citing works
3D bioprinting of tissues and organs
Murphy, S. V., & Atala, A. (2014) · Nature Biotechnology · doi:10.1038/nbt.2958 · Cited by 7,034 · see citing works
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372 · see citing works
Reactive Oxygen Species in Inflammation and Tissue Injury
Mittal, M., Siddiqui, M. R., Tran, K., Reddy, S. P., & Malik, A. B. (2014) · Antioxidants & Redox Signaling · doi:10.1089/ars.2012.5149 · Cited by 4,897 · see citing works
Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology
Peppas, N. A., Hilt, J. Z., Khademhosseini, A., & Langer, R. (2006) · Advanced Materials · doi:10.1002/adma.200501612 · Cited by 4,109 · see citing works
Porous scaffold design for tissue engineering
Hollister, S. J. (2005) · Nature Materials · doi:10.1038/nmat1421 · Cited by 3,979 · see citing works
Biomaterials & scaffolds for tissue engineering
O'Brien, F. J. (2011) · Materials Today · doi:10.1016/s1369-7021(11)70058-x · Cited by 3,437 · see citing works
Applications of machine learning in drug discovery and development
Vamathevan, J., Clark, D., Czodrowski, P., Dunham, I., Ferran, E., Lee, G., Li, B., Madabhushi, A., Shah, P., Spitzer, M., et al. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0024-5 · Cited by 3,228 · see citing works
Organogenesis in a dish: Modeling development and disease using organoid technologies
Lancaster, M. A., & Knoblich, J. A. (2014) · Science · doi:10.1126/science.1247125 · Cited by 2,931 · see citing works
Vascularized and functional human liver from an iPSC-derived organ bud transplant
Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170 · see citing works
A living biobank of breast cancer organoids captures disease heterogeneity
Sachs, N., de Ligt, J., Kopper, O., Gogola, E., Bounova, G., Weeber, F., Balgobind, A. V., Wind, K., Gracanin, A., Begthel, H., et al. (2018) · Cell · doi:10.1016/j.cell.2017.11.010 · Cited by 1,973 · see citing works
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Miller, J. S., Stevens, K. R., Yang, M. T., Baker, B. M., Nguyen, D.-H. T., Cohen, D. M., Toro, E., Chen, A. A., Galie, P. A., Yu, X., et al. (2012) · Nature Materials · doi:10.1038/nmat3357 · Cited by 1,917 · see citing works
25th Anniversary Article: Engineering Hydrogels for Biofabrication
Malda, J., Visser, J., Melchels, F. P., Jüngst, T., Hennink, W. E., Dhert, W. J. A., Groll, J., & Hutmacher, D. W. (2013) · Advanced Materials · doi:10.1002/adma.201302042 · Cited by 1,901 · see citing works
Is it time to start transitioning from 2D to 3D cell culture?
Jensen, C., & Teng, Y. (2020) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2020.00033 · Cited by 1,640 · see citing works
Human primary liver cancer–derived organoid cultures for disease modeling and drug screening
Broutier, L., Mastrogiovanni, G., Verstegen, M. M., Francies, H. E., Gavarró, L. M., Bradshaw, C. R., Allen, G. E., Arnes-Benito, R., Sidorova, O., Gaspersz, M. P., et al. (2017) · Nature Medicine · doi:10.1038/nm.4438 · Cited by 1,471 · see citing works
Printability and shape fidelity of bioinks in 3D bioprinting
Schwab, A., Levato, R., D’Este, M., Piluso, S., Eglin, D., & Malda, J. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00084 · Cited by 1,359 · see citing works
Controlling the Porosity and Microarchitecture of Hydrogels for Tissue Engineering
Annabi, N., Nichol, J. W., Zhong, X., Ji, C., Koshy, S. T., Khademhosseini, A., & Dehghani, F. (2010) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2009.0639 · Cited by 1,315 · see citing works
Engineering organoids
Hofer, M., & Lutolf, M. P. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00279-y · Cited by 1,184 · see citing works
A Patient-Derived Glioblastoma Organoid Model and Biobank Recapitulates Inter- and Intra-tumoral Heterogeneity
Jacob, F., Salinas, R. D., Zhang, D. Y., Nguyen, P. T. T., Schnoll, J. G., Wong, S. Z. H., Thokala, R., Sheikh, S., Saxena, D., Prokop, S., et al. (2020) · Cell · doi:10.1016/j.cell.2019.11.036 · Cited by 1,149 · see citing works
Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids
Matano, M., Date, S., Shimokawa, M., Takano, A., Fujii, M., Ohta, Y., Watanabe, T., Kanai, T., & Sato, T. (2015) · Nature Medicine · doi:10.1038/nm.3802 · Cited by 1,135 · see citing works
Idiosyncratic drug hepatotoxicity
Kaplowitz, N. (2005) · Nature Reviews Drug Discovery · doi:10.1038/nrd1750 · Cited by 1,054 · see citing works
Synthetic alternatives to Matrigel
Aisenbrey, E. A., & Murphy, W. L. (2020) · Nature Reviews Materials · doi:10.1038/s41578-020-0199-8 · Cited by 1,016 · see citing works
Liver regeneration: biological and pathological mechanisms and implications
Michalopoulos, G. K., & Bhushan, B. (2021) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/s41575-020-0342-4 · Cited by 998 · see citing works
Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting
Ma, X., Qu, X., Zhu, W. Z., Li, Y.-S. J., Yuan, S., Zhang, H., Liu, J., Wang, P., Lai, C. S. E., Zanella, F., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1524510113 · Cited by 880 · see citing works
Synthetic polymer scaffolds for tissue engineering
Place, E., George, J. H., Williams, C. K., & Stevens, M. M. (2009) · Chemical Society Reviews · doi:10.1039/b811392k · Cited by 820 · see citing works
Electrospinning for tissue engineering applications
Rahmati, M., Mills, D. K., Urbanska, A. M., Saeb, M. R., Venugopal, J. R., Ramakrishna, S., & Mozafari, M. (2020) · Progress in Materials Science · doi:10.1016/j.pmatsci.2020.100721 · Cited by 816 · see citing works
Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins
Gerets, H. H. J., Tilmant, K., Gerin, B., Chanteux, H., Depelchin, B. O., Dhalluin, S., & Atienzar, F. A. (2012) · Cell Biology and Toxicology · doi:10.1007/s10565-011-9208-4 · Cited by 698 · see citing works
Organoids: A historical perspective of thinking in three dimensions
Simian, M., & Bissell, M. J. (2017) · Journal of Cell Biology · doi:10.1083/jcb.201610056 · Cited by 620 · see citing works
A liver-on-a-chip platform with bioprinted hepatic spheroids
Bhise, N. S., Manoharan, V., Massa, S., Tamayol, A., Ghaderi, M., Miscuglio, M., Lang, Q., Shrike Zhang, Y., Shin, S. R., Calzone, G., et al. (2016) · Biofabrication · doi:10.1088/1758-5090/8/1/014101 · Cited by 615 · see citing works
Global Epidemiology of Chronic Liver Disease
Cheemerla, S., & Balakrishnan, M. (2021) · Clinical Liver Disease · doi:10.1002/cld.1061 · Cited by 571 · see citing works
Three-dimensional plotted scaffolds with controlled pore size gradients: Effect of scaffold geometry on mechanical performance and cell seeding efficiency
Sobral, J. M., Caridade, S. G., Sousa, R. A., Mano, J. F., & Reis, R. L. (2010) · Acta Biomaterialia · doi:10.1016/j.actbio.2010.11.003 · Cited by 561 · see citing works
A review of key challenges of electrospun scaffolds for tissue-engineering applications
Khorshidi, S., Solouk, A., Mirzadeh, H., Mazinani, S., Lagaron, J. M., Sharifi, S., & Ramakrishna, S. (2015) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1978 · Cited by 545 · see citing works
Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids
Ouchi, R., Togo, S., Kimura, M., Shinozawa, T., Koido, M., Koike, H., Thompson, W., Karns, R. A., Mayhew, C. N., McGrath, P. S., et al. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.05.007 · Cited by 537 · see citing works
Polycaprolactone-based biomaterials for tissue engineering and drug delivery: Current scenario and challenges
Mondal, D., Griffith, M., & Venkatraman, S. S. (2016) · International Journal of Polymeric Materials · doi:10.1080/00914037.2015.1103241 · Cited by 513 · see citing works
Reproducing human and cross-species drug toxicities using a liver-chip
Jang, K.-J., Otieno, M. A., Ronxhi, J., Lim, H.-K., Ewart, L., Kodella, K. R., Petropolis, D. B., Kulkarni, G., Rubins, J. E., Conegliano, D., et al. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aax5516 · Cited by 511 · see citing works
An artificial liver sinusoid with a microfluidic endothelial‐like barrier for primary hepatocyte culture
Lee, P. J., Hung, P. J., & Lee, L. P. (2007) · Biotechnology and Bioengineering · doi:10.1002/bit.21360 · Cited by 507 · see citing works
Drug development in the era of precision medicine
Dugger, S. A., Platt, A., & Goldstein, D. B. (2018) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2017.226 · Cited by 493 · see citing works
Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering
Lu, T., Li, Y., & Chen, T. (2013) · International Journal of Nanomedicine · doi:10.2147/ijn.s38635 · Cited by 478 · see citing works
Bioprinting of human pluripotent stem cells and their directed differentiation into hepatocyte-like cells for the generation of mini-livers in 3D
Faulkner‐Jones, A., Fyfe, C., Cornelissen, D.-J., Gardner, J., King, J., Courtney, A., & Shu, W. (2015) · Biofabrication · doi:10.1088/1758-5090/7/4/044102 · Cited by 477 · see citing works
Organoid Models of Human Liver Cancers Derived from Tumor Needle Biopsies
Nuciforo, S., Fofana, I., Matter, M. S., Blumer, T., Calabrese, D., Boldanova, T., Piscuoglio, S., Wieland, S., Ringnalda, F., Schwank, G., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.07.001 · Cited by 473 · see citing works
Open TG-GATEs: a large-scale toxicogenomics database
Igarashi, Y., Nakatsu, N., Yamashita, T., Ono, A., Ohno, Y., Urushidani, T., & Yamada, H. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku955 · Cited by 465 · see citing works
3D Bioprinting: from Benches to Translational Applications
Heinrich, M. A., Liu, W. H., Jimenez, A., Yang, J., Akpek, A., Liu, X., Pi, Q., Mu, X., Hu, N., Schiffelers, R. M., et al. (2019) · Small · doi:10.1002/smll.201805510 · Cited by 445 · see citing works
Commercialization of microfluidic devices
Volpatti, L. R., & Yetisen, A. K. (2014) · Trends in biotechnology · doi:10.1016/j.tibtech.2014.04.010 · Cited by 437 · see citing works
A microfluidic 3D hepatocyte chip for drug toxicity testing
Toh, Y., Lim, T. C., Tai, D., Xiao, G., van Noort, D., & Yu, H. (2009) · Lab on a Chip · doi:10.1039/b900912d · Cited by 422 · see citing works
Is it possible to overcome issues of external validity in preclinical animal research? Why most animal models are bound to fail
Pound, P., & Ritskes-Hoitinga, M. (2018) · Journal of Translational Medicine · doi:10.1186/s12967-018-1678-1 · Cited by 419 · see citing works
Organoids by design
Takebe, T., & Wells, J. M. (2019) · Science · doi:10.1126/science.aaw7567 · Cited by 417 · see citing works
Towards organoid culture without Matrigel
Kozlowski, M. T., Crook, C. J., & Ku, H. T. (2021) · Communications Biology · doi:10.1038/s42003-021-02910-8 · Cited by 409 · see citing works
3D bioprinting of functional tissue models for personalized drug screening and in vitro disease modeling
Ma, X., Liu, J., Zhu, W. Z., Tang, M., Lawrence, N., Yu, C., Gou, M., & Chen, S. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.06.011 · Cited by 408 · see citing works
Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells
Takebe, T., Sekine, K., Kimura, M., Yoshizawa, E., Ayano, S., Koido, M., Funayama, S., Nakanishi, N., Hisai, T., Kobayashi, T., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.11.005 · Cited by 407 · see citing works
Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation
Sampaziotis, F., de Brito, M. C., Madrigal, P., Bertero, A., Saeb‐Parsy, K., Soares, F. A. C., Schrumpf, E., Melum, E., Karlsen, T. H., Bradley, J. A., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3275 · Cited by 398 · see citing works
Principals of neovascularization for tissue engineering
Nomi, M., Atala, A., Coppi, P. D., & Söker, S. (2002) · Molecular Aspects of Medicine · doi:10.1016/s0098-2997(02)00008-0 · Cited by 392 · see citing works
Emerging Biofabrication Strategies for Engineering Complex Tissue Constructs
Pedde, R. D., Mirani, B., Navaei, A., Styan, T., Wong, S., Mehrali, M., Thakur, A., Mohtaram, N. K., Bayati, A., Dolatshahi‐Pirouz, A., et al. (2017) · Advanced Materials · doi:10.1002/adma.201606061 · Cited by 392 · see citing works
Bioprinted 3D Primary Liver Tissues Allow Assessment of Organ-Level Response to Clinical Drug Induced Toxicity In Vitro
Nguyen, D. G., Funk, J., Robbins, J. B., Crogan‐Grundy, C., Presnell, S. C., Singer, T., & Roth, A. B. (2016) · PLoS ONE · doi:10.1371/journal.pone.0158674 · Cited by 385 · see citing works
High-Fidelity Drug-Induced Liver Injury Screen Using Human Pluripotent Stem Cell–Derived Organoids
Shinozawa, T., Kimura, M., Cai, Y., Saiki, N., Yoneyama, Y., Ouchi, R., Koike, H., Maezawa, M., Zhang, R.-R., Dunn, A., et al. (2021) · Gastroenterology · doi:10.1053/j.gastro.2020.10.002 · Cited by 378 · see citing works
Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids
Mun, S. J., Ryu, J., Lee, M., Son, Y. S., Oh, S. J., Cho, H., Son, M., Kim, S. J., Kim, S. J., Yoo, H. J., et al. (2019) · Journal of Hepatology · doi:10.1016/j.jhep.2019.06.030 · Cited by 369 · see citing works
Liver organoids: from basic research to therapeutic applications
Prior, N., Inacio, P., & Huch, M. (2019) · Gut · doi:10.1136/gutjnl-2019-319256 · Cited by 356 · see citing works
Generation of Three-Dimensional Hepatocyte/Gelatin Structures with Rapid Prototyping System
Wang, X., Yan, Y., Pan, Y., Xiong, Z., Liu, H., Cheng, J., Liu, F., Lin, F., Wu, R., Zhang, R., et al. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.83 · Cited by 353 · see citing works
Hydrogel microenvironments for cancer spheroid growth and drug screening
Li, Y., & Kumacheva, E. (2018) · Science Advances · doi:10.1126/sciadv.aas8998 · Cited by 349 · see citing works
Microfluidic-enhanced 3D bioprinting of aligned myoblast-laden hydrogels leads to functionally organized myofibers in vitro and in vivo
Costantini, M., Testa, S., Mozetic, P., Barbetta, A., Fuoco, C., Fornetti, E., Tamiro, F., Bernardini, S., Jaroszewicz, J., Święszkowski, W., et al. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.03.026 · Cited by 334 · see citing works
Self-citation
Development of Liver Decellularized Extracellular Matrix Bioink for Three-Dimensional Cell Printing-Based Liver Tissue Engineering
Lee, H., Han, W., Kim, H., Ha, D., Jang, J., Kim, B. S., & Cho, D. (2017) · Biomacromolecules · doi:10.1021/acs.biomac.6b01908 · Cited by 329 · see citing works
Utility of spherical human liver microtissues for prediction of clinical drug-induced liver injury
Proctor, W. R., Foster, A. J., Vogt, J., Summers, C., Middleton, B., Pilling, M. A., Shienson, D., Kijanska, M., Ströbel, S., Kelm, J. M., et al. (2017) · Archives of Toxicology · doi:10.1007/s00204-017-2002-1 · Cited by 328 · see citing works
Bio-inspired detoxification using 3D-printed hydrogel nanocomposites
Gou, M., Qu, X., Zhu, W. Z., Xiang, M., Yang, J., Zhang, K., Wei, Y., & Chen, S. (2014) · Nature Communications · doi:10.1038/ncomms4774 · Cited by 324 · see citing works
Directed differentiation of cholangiocytes from human pluripotent stem cells
Ogawa, M., Ogawa, S., Bear, C. E., Ahmadi, S., Chin, S., Li, B., Grompe, M., Keller, G. M., Kamath, B. M., & Ghanekar, A. (2015) · Nature Biotechnology · doi:10.1038/nbt.3294 · Cited by 318 · see citing works
Structural and functional hepatocyte polarity and liver disease
Gissen, P., & Arias, I. M. (2015) · Journal of Hepatology · doi:10.1016/j.jhep.2015.06.015 · Cited by 316 · see citing works
Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver‐Like Metabolic Biofactories
Bernal, P. N., Bouwmeester, M., Madrid-Wolff, J. A., Falandt, M., Florczak, S., Rodriguez, N. G., Li, Y., Größbacher, G., Samsom, R., van Wolferen, M. E., et al. (2022) · Advanced Materials · doi:10.1002/adma.202110054 · Cited by 290 · see citing works
Growth of Epithelial Organoids in a Defined Hydrogel
Broguiere, N., Isenmann, L., Hirt, C., Ringel, T., Placzek, S., Cavalli, E., Ringnalda, F., Villiger, L., Züllig, R., Lehmann, R., et al. (2018) · Advanced Materials · doi:10.1002/adma.201801621 · Cited by 282 · see citing works
Rapid 3D bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture
Ma, X., Yu, C., Wang, P., Xu, W., Wan, X., Lai, C. S. E., Liu, J., Koroleva-Maharajh, A., & Chen, S. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.09.026 · Cited by 281 · see citing works
Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease
Ramli, M. N. B., Lim, Y. S., Koe, C. T., Demircioglu, D., Tng, W., Gonzales, K. A. U., Tan, C. P., Szczerbinska, I., Liang, H., Soe, E. L., et al. (2020) · Gastroenterology · doi:10.1053/j.gastro.2020.06.010 · Cited by 278 · see citing works
A microfluidically perfused three dimensional human liver model
Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., et al. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.043 · Cited by 258 · see citing works
Reciprocal modulation of growth and differentiated functions of mature rat hepatocytes in primary culture by cell--cell contact and cell membranes.
Nakamura, T., Yoshimoto, K., Nakayama, Y., Tomita, Y., & Ichihara, A. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.23.7229 · Cited by 256 · see citing works
Development of a 3D cell printed construct considering angiogenesis for liver tissue engineering
Lee, J. W., Choi, Y., Yong, W.-J., Pati, F., Shim, J., Kang, K. S., Kang, I.-H., Park, J., & Cho, D. (2016) · Biofabrication · doi:10.1088/1758-5090/8/1/015007 · Cited by 255 · see citing works
Hepatocyte polarity
Treyer, A., & Müsch, A. (2013) · Comprehensive Physiology · doi:10.1002/cphy.c120009 · Cited by 252 · see citing works
Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions
Yamada, M., Utoh, R., Ohashi, K., Tatsumi, K., Yamato, M., Okano, T., & Seki, M. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.07.068 · Cited by 247 · see citing works
Animal models for liver disease – A practical approach for translational research
Nevzorova, Y. A., Boyer‐Díaz, Z., Cubero, F. J., & Gracia‐Sancho, J. (2020) · Journal of Hepatology · doi:10.1016/j.jhep.2020.04.011 · Cited by 247 · see citing works
Human hepatic organoids for the analysis of human genetic diseases
Guan, Y., Xu, D., Garfin, P. M., Ehmer, U., Hurwitz, M., Enns, G., Michie, S., Wu, M., Zheng, M., Nishimura, T., et al. (2017) · JCI Insight · doi:10.1172/jci.insight.94954 · Cited by 243 · see citing works
The Current Status of Drug Discovery and Development as Originated in United States Academia: The Influence of Industrial and Academic Collaboration on Drug Discovery and Development
Takebe, T., Imai, R., & Ono, S. (2018) · Clinical and Translational Science · doi:10.1111/cts.12577 · Cited by 239 · see citing works
Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro
Leite, S. B., Roosens, T., El Taghdouini, A., Mannaerts, I., Smout, A. J., Najimi, M., Sokal, E., Noor, F., Chesne, C., & van Grunsven, L. A. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2015.11.026 · Cited by 234 · see citing works
Generation of Hepatic Stellate Cells from Human Pluripotent Stem Cells Enables In Vitro Modeling of Liver Fibrosis
Coll, M., Perea, L., Boon, R., Leite, S. B., Vallverdú, J., Mannaerts, I., Smout, A., El Taghdouini, A., Blaya, D., Rodrigo-Torres, D., et al. (2018) · Cell Stem Cell · doi:10.1016/j.stem.2018.05.027 · Cited by 233 · see citing works
Engineered Liver-On-A-Chip Platform to Mimic Liver Functions and Its Biomedical Applications: A Review
Deng, J., Wei, W., Chen, Z., Lin, B., Zhao, W., Luo, Y., & Zhang, X. (2019) · Micromachines · doi:10.3390/mi10100676 · Cited by 232 · see citing works
A human liver microphysiology platform for investigating physiology, drug safety, and disease models
Vernetti, L. A., Senutovitch, N., Boltz, R. C., DeBiasio, R., Shun, T. Y., Gough, A., & Taylor, D. L. (2015) · Experimental Biology and Medicine · doi:10.1177/1535370215592121 · Cited by 230 · see citing works
Liver-cell patterning lab chip: mimicking the morphology of liver lobule tissue
Ho, C.-T., Lin, R.-Z., Chen, R.-J., Chin, C.-K., Gong, S.-E., Chang, H.-Y., Peng, H.-L., Hsu, L., Yew, T.-R., Chang, S.-F., et al. (2013) · Lab on a Chip · doi:10.1039/c3lc50402f · Cited by 230 · see citing works
Cell sources for <i>in vitro</i> human liver cell culture models
Zeilinger, K., Freyer, N., Damm, G., Seehofer, D., & Knöspel, F. (2016) · Experimental Biology and Medicine · doi:10.1177/1535370216657448 · Cited by 230 · see citing works
Fabrication of liver microtissue with liver decellularized extracellular matrix (dECM) bioink by digital light processing (DLP) bioprinting
Mao, Q., Wang, Y., Li, Y., Juengpanich, S., Li, W., Chen, M., Yin, J., Fu, J., & Cai, X. (2020) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.110625 · Cited by 219 · see citing works
Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids
Artegiani, B., van Voorthuijsen, L., Lindeboom, R. G. H., Seinstra, D., Heo, I., Tapia, P., López-Iglesias, C., Postrach, D., Dayton, T., Oka, R., et al. (2019) · Cell Stem Cell · doi:10.1016/j.stem.2019.04.017 · Cited by 218 · see citing works
Human primary liver cancer organoids reveal intratumor and interpatient drug response heterogeneity
Li, L., Knútsdóttir, H., Hui, K., Weiss, M. J., He, J. P., Philosophe, B., Cameron, A. M., Wolfgang, C. L., Pawlik, T. M., Ghiaur, G., et al. (2019) · JCI Insight · doi:10.1172/jci.insight.121490 · Cited by 216 · see citing works
Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells
Dianat, N., Dubois‐Pot‐Schneider, H., Steichen, C., Desterke, C., Leclerc, P., Raveux, A., Combettes, L., Weber, A., Corlu, A., & Dubart‐Kupperschmitt, A. (2014) · Hepatology · doi:10.1002/hep.27165 · Cited by 213 · see citing works
Liver ‘organ on a chip’
Beckwitt, C. H., Clark, A. M., Wheeler, S., Taylor, D. L., Stolz, D. B., Griffith, L. G., & Wells, A. H. (2017) · Experimental Cell Research · doi:10.1016/j.yexcr.2017.12.023 · Cited by 211 · see citing works
Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury
Wang, S., Wang, X., Tan, Z., Su, Y., Liu, J., Chang, M., Yan, F., Chen, J., Chen, T., Li, C., et al. (2019) · Cell Research · doi:10.1038/s41422-019-0242-8 · Cited by 211 · see citing works
Seminars in Liver Disease
Huebert, A. A. A. N. J.-S. R. C. (2026) · Seminars in Liver Disease · doi:10.1055/s-00000069 · Cited by 210 · see citing works
Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies
Castell, J. V., Jover, R., Martinez‐Jimenez, C. P., & G�mez-Lech�n, M. J. (2006) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425255.2.2.183 · Cited by 209 · see citing works
3D bioprinting of liver-mimetic construct with alginate/cellulose nanocrystal hybrid bioink
Wu, Y., Lin, Z. Y., Wenger, A., Tam, K. C., & Tang, X. S. (2017) · Bioprinting · doi:10.1016/j.bprint.2017.12.001 · Cited by 209 · see citing works
Mediators of Inflammation
Galvão, I., Sugimoto, M. A., Vago, J. P., Machado, M. G., & Sousa, L. P. (2018) · Springer eBooks · doi:10.1007/978-3-319-77658-3_1 · Cited by 202 · see citing works
Animal models of drug-induced liver injury
McGill, M. R., & Jaeschke, H. (2018) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2018.08.037 · Cited by 202 · see citing works
A 3D microfluidic liver model for high throughput compound toxicity screening in the OrganoPlate®
Bircsak, K. M., DeBiasio, R., Miedel, M. T., Alsebahi, A., Reddinger, R. M., Saleh, A. D., Shun, T., Vernetti, L. A., & Gough, A. (2021) · Toxicology · doi:10.1016/j.tox.2020.152667 · Cited by 198 · see citing works
Bioprinting towards physiologically relevant tissue models for pharmaceutics
Peng, W., Unutmaz, D., & Ozbolat, I. T. (2016) · Trends in Biotechnology · doi:10.1016/j.tibtech.2016.05.013 · Cited by 196 · see citing works
Fabrication of scalable and structured tissue engineering scaffolds using water dissolvable sacrificial 3D printed moulds
Mohanty, S., Larsen, L. B., Trifol, J., Szabó, P., Burri, H., Canali, C., Dufva, M., Emnéus, J., & Wolff, A. (2015) · Materials Science and Engineering C · doi:10.1016/j.msec.2015.06.002 · Cited by 194 · see citing works
Cell-printed 3D liver-on-a-chip possessing a liver microenvironment and biliary system
Lee, H., Chae, S., Kim, J. Y., Han, W., Kim, J., Choi, Y., & Cho, D. (2018) · Biofabrication · doi:10.1088/1758-5090/aaf9fa · Cited by 194 · see citing works
Recapitulation of hepatitis B virus–host interactions in liver organoids from human induced pluripotent stem cells
Nie, Y., Zheng, Y., Miyakawa, K., Murata, S., Zhang, R., Sekine, K., Ueno, Y., Takebe, T., Wakita, T., Ryo, A., et al. (2018) · EBioMedicine · doi:10.1016/j.ebiom.2018.08.014 · Cited by 187 · see citing works
Direct Fabrication of a Hybrid Cell/Hydrogel Construct by a Double-nozzle Assembling Technology
Li, S., Xiong, Z., Wang, X., Yan, Y., Liu, H., & Zhang, R. (2009) · Journal of Bioactive and Compatible Polymers · doi:10.1177/0883911509104094 · Cited by 181 · see citing works
Microengineered Multi‐Organoid System from hiPSCs to Recapitulate Human Liver‐Islet Axis in Normal and Type 2 Diabetes
Tao, T., Deng, P., Wang, Y., Zhang, X., Guo, Y., Chen, W., & Qin, J. (2022) · Advanced Science · doi:10.1002/advs.202103495 · Cited by 178 · see citing works
In vitro analysis of a hepatic device with intrinsic microvascular-based channels
Carraro, A., Hsu, W.-M., Kulig, K. M., Cheung, W. S., Miller, M. L., Weinberg, E. J., Swart, E. F., Kaazempur-Mofrad, M., Borenstein, J. T., Vacanti, J. P., et al. (2008) · Biomedical Microdevices · doi:10.1007/s10544-008-9194-3 · Cited by 168 · see citing works
3D-printed gelatin scaffolds of differing pore geometry modulate hepatocyte function and gene expression
Lewis, P. L., Green, R. M., & Shah, R. N. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.12.042 · Cited by 168 · see citing works
Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies
Underhill, G. H., & Khetani, S. R. (2017) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2017.11.012 · Cited by 167 · see citing works
Cell Plasticity in Liver Regeneration
Li, W., Li, L., & Hui, L. (2020) · Trends in Cell Biology · doi:10.1016/j.tcb.2020.01.007 · Cited by 167 · see citing works
Liver Zonation – Revisiting Old Questions With New Technologies
Cunningham, R. P., & Porat‐Shliom, N. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.732929 · Cited by 166 · see citing works
Novel wet electrospinning system for fabrication of spongiform nanofiber 3-dimensional fabric
Yokoyama, Y., Hattori, S., Yoshikawa, C., Yasuda, Y., Koyama, H., Takato, T., & Kobayashi, H. (2009) · Materials Letters · doi:10.1016/j.matlet.2008.12.042 · Cited by 165 · see citing works
A Chemically Defined Hydrogel for Human Liver Organoid Culture
Ye, S., Boeter, J. W. B., Mihajlovic, M., van Steenbeek, F. G., van Wolferen, M. E., Oosterhoff, L. A., Marsee, A., Caiazzo, M., van der Laan, L. J. W., Penning, L. C., et al. (2020) · Advanced Functional Materials · doi:10.1002/adfm.202000893 · Cited by 165 · see citing works
Organoids to model liver disease
Nuciforo, S., & Heim, M. H. (2021) · JHEP Reports · doi:10.1016/j.jhepr.2020.100198 · Cited by 164 · see citing works
Hydrogel-Based Fiber Biofabrication Techniques for Skeletal Muscle Tissue Engineering
Volpi, M., Paradiso, A., Costantini, M., & Święszkowski, W. (2022) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c01145 · Cited by 164 · see citing works
Self-citation
Hepatic organoids for microfluidic drug screening
Au, S. H., Chamberlain, M. D., Mahesh, S., Sefton, M. V., & Wheeler, A. R. (2014) · Lab on a Chip · doi:10.1039/c4lc00531g · Cited by 162 · see citing works
Vascularized Liver Organoids Generated Using Induced Hepatic Tissue and Dynamic Liver‐Specific Microenvironment as a Drug Testing Platform
Jin, Y., Kim, J., Lee, J. S., Min, S., Kim, S., Ahn, D., Kim, Y., & Cho, S. (2018) · Advanced Functional Materials · doi:10.1002/adfm.201801954 · Cited by 161 · see citing works
3D biofabrication of vascular networks for tissue regeneration: A report on recent advances
Sarker, M. D., Naghieh, S., Sharma, N. K., & Chen, D. (2018) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2018.08.005 · Cited by 159 · see citing works
Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling
Akbari, S., Sevinç, G. G., Ersoy, N., Başak, O., Kaplan, K. N., Sevinç, K., Ozel, E., Şengün, B., Enüstün, E., Özçimen, B., et al. (2019) · Stem Cell Reports · doi:10.1016/j.stemcr.2019.08.007 · Cited by 158 · see citing works
Direct Construction of a Three-dimensional Structure with Cells and Hydrogel
Yan, Y., Wang, X., Xiong, Z., Liu, H., Liu, F., Lin, F., Wu, R., Zhang, R., & Lu, Q. (2005) · Journal of Bioactive and Compatible Polymers · doi:10.1177/0883911505053658 · Cited by 155 · see citing works
3D in vitro models of liver fibrosis
van Grunsven, L. A. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.07.004 · Cited by 154 · see citing works
Bioprinted 3D Primary Human Intestinal Tissues Model Aspects of Native Physiology and ADME/Tox Functions
Madden, L. R., Nguyen, T. V., Garcia-Mojica, S., Shah, V., Le, A. V., Peier, A. M., Visconti, R. J., Parker, E. M., Presnell, S. C., Nguyen, D. G., et al. (2018) · iScience · doi:10.1016/j.isci.2018.03.015 · Cited by 154 · see citing works
A novel system for evaluation of drug mixtures for potential efficacy in treating multidrug resistant cancers
Tatosian, D. A., & Shuler, M. L. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.22219 · Cited by 149 · see citing works
Three-dimensional liver-derived extracellular matrix hydrogel promotes liver organoids function
Saheli, M., Sepantafar, M., Pournasr, B., Farzaneh, Z., Vosough, M., Piryaei, A., & Baharvand, H. (2018) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26622 · Cited by 148 · see citing works
Three-dimensional bio-printing of primary human hepatocellular carcinoma for personalized medicine
Xie, F., Sun, L., Pang, Y., Xu, G., Jin, B., Xu, H., Lu, X., Xu, Y., Du, S., Wang, Y., et al. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2020.120416 · Cited by 147 · see citing works
Online oxygen monitoring using integrated inkjet-printed sensors in a liver-on-a-chip system
Moya, A., Ortega‐Ribera, M., Villalba, X. G., Sowade, E., Zea, M., Illa, X., Ramón, E., Villa, R., Gracia‐Sancho, J., & Gabriel, G. (2018) · Lab on a Chip · doi:10.1039/c8lc00456k · Cited by 146 · see citing works
Development of a biomimetic liver tumor-on-a-chip model based on decellularized liver matrix for toxicity testing
Lu, S., Cuzzucoli, F., Jiang, J., Liang, L.-G., Wang, Y., Kong, M., Zhao, X., Cui, W., Li, J., & Wang, S. (2018) · Lab on a Chip · doi:10.1039/c8lc00852c · Cited by 144 · see citing works
Control of oxygen tension recapitulates zone-specific functions in human liver microphysiology systems
Lee-Montiel, F. T., George, S. M., Gough, A., Sharma, A. D., Wu, J., DeBiasio, R., Vernetti, L. A., & Taylor, D. L. (2017) · Experimental Biology and Medicine · doi:10.1177/1535370217703978 · Cited by 141 · see citing works
Generation of a 3D Liver Model Comprising Human Extracellular Matrix in an Alginate/Gelatin-Based Bioink by Extrusion Bioprinting for Infection and Transduction Studies
Hiller, T., Berg, J., Elomaa, L., Röhrs, V., Ullah, I., Schaar, K., Dietrich, A.-C., Al‐Zeer, M. A., Kurtz, A., Hocke, A. C., et al. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19103129 · Cited by 141 · see citing works
Human stem cell-derived hepatocytes as a model for hepatitis B virus infection, spreading and virus-host interactions
Xia, Y., Carpentier, A., Cheng, X., Block, P. D., Zhao, Y., Zhang, Z., Protzer, U., & Liang, T. J. (2016) · Journal of Hepatology · doi:10.1016/j.jhep.2016.10.009 · Cited by 139 · see citing works
Liver-Tumor Hybrid Organoids for Modeling Tumor Growth and Drug Response In Vitro
Skardal, A., Devarasetty, M., Rodman, C., Atala, A., & Söker, S. (2015) · Annals of Biomedical Engineering · doi:10.1007/s10439-015-1298-3 · Cited by 138 · see citing works
A critical perspective on 3D liver models for drug metabolism and toxicology studies
Serras, A. S., Rodrigues, J. S., Cipriano, M., Rodrigues, A. V., Oliveira, N. G., & Miranda, J. P. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.626805 · Cited by 138 · see citing works
3D Primary Hepatocyte Culture Systems for Analyses of Liver Diseases, Drug Metabolism, and Toxicity: Emerging Culture Paradigms and Applications
Lauschke, V. M., Shafagh, R. Z., Hendriks, D. F. G., & Ingelman‐Sundberg, M. (2019) · Biotechnology Journal · doi:10.1002/biot.201800347 · Cited by 135 · see citing works
On being the right size: scaling effects in designing a human-on-a-chip
Moraes, C., Labuz, J. M., Leung, B. M., Inoue, M., Chun, T.-H., & Takayama, S. (2013) · Integrative Biology · doi:10.1039/c3ib40040a · Cited by 134 · see citing works
Human iPS derived progenitors bioengineered into liver organoids using an inverted colloidal crystal poly (ethylene glycol) scaffold
Ng, S. S., Saeb-Parsy, K., Blackford, S. J. I., Segal, J. M., Serra, M. P., Horcas-Lopez, M., No, D. Y., Mastoridis, S., Jassem, W., Frank, C. W., et al. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.07.043 · Cited by 133 · see citing works
3D bioprinting of liver spheroids derived from human induced pluripotent stem cells sustain liver function and viability in vitro
Goulart, E., de Caires-Junior, L. C., Telles-Silva, K. A., Araújo, B. H. S., Rocco, S. A., Sforça, M. L., de Sousa, I. L., Kobayashi, G. S., Musso, C. M., Assoni, A. F., et al. (2019) · Biofabrication · doi:10.1088/1758-5090/ab4a30 · Cited by 133 · see citing works
Microfluidic platform for real-time signaling analysis of multiple single T cells in parallel
Faley, S. L., Seale, K. T., Hughey, J., Schaffer, D. K., VanCompernolle, S. E., McKinney, B. A., Baudenbacher, F. J., Unutmaz, D., & Wikswo, J. P. (2008) · Lab on a Chip · doi:10.1039/b719799c · Cited by 132 · see citing works
3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model
Cuvellier, M., Ezan, F., Oliveira, H., Rose, S., Fricain, J.-C., Langouët, S., Legagneux, V., & Baffet, G. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2020.120611 · Cited by 126 · see citing works
A perfusion incubator liver chip for 3D cell culture with application on chronic hepatotoxicity testing
Yu, F., Deng, R., Tong, W. H., Li, H., Ng, C. W., IslamBadhan, A., Iliescu, C., & Yu, H. (2017) · Scientific Reports · doi:10.1038/s41598-017-13848-5 · Cited by 125 · see citing works
Nanofibrous PLGA electrospun scaffolds modified with type I collagen influence hepatocyte function and support viability in vitro
Brown, J. H., Das, P., DiVito, M. D., Ivancic, D., Tan, L. P., & Wertheim, J. A. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.02.009 · Cited by 114 · see citing works
Preparation, characterization, and evaluation of genipin crosslinked chitosan/gelatin three-dimensional scaffolds for liver tissue engineering applications
Zhang, Y., Wang, Q., Yan, K., Qi, Y., Wang, G., & Cui, Y. (2016) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35717 · Cited by 110 · see citing works
Prediction of human drug-induced liver injury (DILI) in relation to oral doses and blood concentrations
Albrecht, W., Kappenberg, F., Brecklinghaus, T., Stöber, R. M., Marchan, R., Zhang, M., Ebbert, K. E., Kirschner, H., Grinberg, M., Leist, M., et al. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02492-9 · Cited by 110 · see citing works
Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research
Sun, L., Yang, H., Wang, Y., Zhang, X., Jin, B., Xie, F., Jin, Y., Pang, Y., Zhao, H., Lu, X., et al. (2020) · Frontiers in Oncology · doi:10.3389/fonc.2020.00878 · Cited by 110 · see citing works
A transcriptomics data-driven gene space accurately predicts liver cytopathology and drug-induced liver injury
Kohonen, P. J., Parkkinen, J., Willighagen, E., Ceder, R., Wennerberg, K., Samuel Kaski, A., & Grafström, R. (2017) · Nature Communications · doi:10.1038/ncomms15932 · Cited by 109 · see citing works
Organoid biobanking: identifying the ethics
Boers, S. N., van Delden, J. J. M., Clevers, H. C., & Bredenoord, A. L. (2016) · EMBO Reports · doi:10.15252/embr.201642613 · Cited by 106 · see citing works
Drug-Induced Liver Injury: Biomarkers, Requirements, Candidates, and Validation
Meunier, L., & Larrey, D. G. (2019) · Frontiers in Pharmacology · doi:10.3389/fphar.2019.01482 · Cited by 106 · see citing works
Polymer Hydrogels to Guide Organotypic and Organoid Cultures
Magno, V., Meinhardt, A., & Werner, C. (2020) · Advanced Functional Materials · doi:10.1002/adfm.202000097 · Cited by 106 · see citing works
Extending Hepatocyte Functionality for Drug-Testing Applications Using High-Viscosity Alginate–Encapsulated Three-Dimensional Cultures in Bioreactors
Miranda, J. P., Rodrigues, A., Tostões, R. M., Leite, S. B., Zimmerman, H., Carrondo, M. J. T., & Alves, P. M. (2010) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2009.0784 · Cited by 102 · see citing works
Human Nonalcoholic Steatohepatitis on a Chip
Freag, M. S., Namgung, B., Reyna Fernandez, M. E., Gherardi, E., Sengupta, S., & Jang, H. L. (2021) · Hepatology Communications · doi:10.1002/hep4.1647 · Cited by 102 · see citing works
A comprehensive evaluation of metabolic activity and intrinsic clearance in suspensions and monolayer cultures of cryopreserved primary human hepatocytes
Smith, C. M., Nolan, C. K., Edwards, M., Hatfield, J. B., Stewart, T. W., Ferguson, S. S., LeCluyse, E. L., & Sahi, J. (2012) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.23262 · Cited by 99 · see citing works
Grafting of 3D Bioprinting to In Vitro Drug Screening: A Review
Nie, J., Gao, Q., Fu, J., & He, Y. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.201901773 · Cited by 99 · see citing works
On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro
Jang, M., Neužil, P., Volk, T. J., Manz, A., & Kleber, A. (2015) · Biomicrofluidics · doi:10.1063/1.4922863 · Cited by 98 · see citing works
Three-dimensional printing of chemically crosslinked gelatin hydrogels for adipose tissue engineering
Negrini, N. C., Celikkin, N., Tarsini, P., Farè, S., & Święszkowski, W. (2019) · Biofabrication · doi:10.1088/1758-5090/ab56f9 · Cited by 98 · see citing works
Self-citation
Mimicking Native Liver Lobule Microarchitecture In Vitro with Parenchymal and Non-parenchymal Cells Using 3D Bioprinting for Drug Toxicity and Drug Screening Applications
Janani, G., Priya, S., Dey, S., & Mandal, B. B. (2022) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.2c00312 · Cited by 98 · see citing works
3D bioprinting of bicellular liver lobule-mimetic structures via microextrusion of cellulose nanocrystal-incorporated shear-thinning bioink
Wu, Y., Wenger, A., Golzar, H., & Tang, X. S. (2020) · Scientific Reports · doi:10.1038/s41598-020-77146-3 · Cited by 95 · see citing works
DeepDILI: Deep Learning-Powered Drug-Induced Liver Injury Prediction Using Model-Level Representation
Li, T., Tong, W., Roberts, R. A., Liu, Z., & Thakkar, S. (2020) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.0c00374 · Cited by 94 · see citing works
A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues
Klotz, B. J., Oosterhoff, L. A., Utomo, L., Lim, K. S., Vallmajó-Martín, Q., Clevers, H. C., Woodfield, T. B. F., Rosenberg, A. J. W. P., Malda, J., Ehrbar, M., et al. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201900979 · Cited by 93 · see citing works
Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids
Krüger, M., Oosterhoff, L. A., van Wolferen, M. E., Schiele, S., Walther, A., Geijsen, N., Laporte, L. D., van der Laan, L. J. W., Kock, L. M., & Spee, B. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.201901658 · Cited by 91 · see citing works
Blended electrospinning with human liver extracellular matrix for engineering new hepatic microenvironments
Grant, R., Hallett, J. M., Forbes, S. J., Hay, D. C., & Callanan, A. (2019) · Scientific Reports · doi:10.1038/s41598-019-42627-7 · Cited by 90 · see citing works
Modeling alcohol-associated liver disease in a human Liver-Chip
Nawroth, J. C., Petropolis, D. B., Manatakis, D. V., Maulana, T. I., Burchett, G., Schlünder, K., Witt, A., Shukla, A., Kodella, K., Ronxhi, J., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109393 · Cited by 88 · see citing works
In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device
Skardal, A., Devarasetty, M., Söker, S., & Hall, A. R. (2015) · Biofabrication · doi:10.1088/1758-5090/7/3/031001 · Cited by 86 · see citing works
Degradable hydrogels derived from PEG‐diacrylamide for hepatic tissue engineering
Stevens, K. R., Miller, J. S., Blakely, B. L., Chen, C. S., & Bhatia, S. N. (2015) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35478 · Cited by 83 · see citing works
Liver Tissue Engineering: Challenges and Opportunities
Agarwal, T., Subramanian, B., & Maiti, T. K. (2019) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.9b00745 · Cited by 83 · see citing works
Organoid culture media formulated with growth factors of defined cellular activity
Urbischek, M., Rannikmäe, H., Foets, T., Ravn, K., Hyvönen, M., & de la Roche, M. (2019) · Scientific Reports · doi:10.1038/s41598-019-42604-0 · Cited by 81 · see citing works
Bioprinted liver provides early insight into the role of Kupffer cells in TGF-β1 and methotrexate-induced fibrogenesis
Norona, L. M., Nguyen, D. G., Gerber, D. A., Presnell, S. C., Mosedale, M., & Watkins, P. B. (2019) · PLoS ONE · doi:10.1371/journal.pone.0208958 · Cited by 79 · see citing works
One-step synthesis of composite hydrogel capsules to support liver organoid generation from hiPSCs
Wang, Y., Liu, H., Zhang, M., Wang, H., Chen, W., & Qin, J. (2020) · Biomaterials Science · doi:10.1039/d0bm01085e · Cited by 78 · see citing works
Injectable Liver: A Novel Approach Using Fibrin Gel as a Matrix for Culture and Intrahepatic Transplantation of Hepatocytes
Bruns, H., Kneser, U., Holzhüter, S., Roth, B., Kluth, J., Kaufmann, P. M., Kluth, D., & Fiegel, H. C. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.1718 · Cited by 77 · see citing works
Consent for governance in the ethical use of organoids
Boers, S. N., & Bredenoord, A. L. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0112-5 · Cited by 77 · see citing works
Small RNAs in eucaryotes: new clues for amplifying microRNA benefits
Ledda, B., Ottaggio, L., Izzotti, A., Sukkar, S. G., & Miele, M. (2020) · Cell & Bioscience · doi:10.1186/s13578-019-0370-3 · Cited by 76 · see citing works
Biofabrication of Hepatic Constructs by 3D Bioprinting of a Cell‐Laden Thermogel: An Effective Tool to Assess Drug‐Induced Hepatotoxic Response
Gori, M., Giannitelli, S. M., Torre, M., Mozetic, P., Abbruzzese, F., Trombetta, M., Traversa, E., Moroni, L., & Rainer, A. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.202001163 · Cited by 76 · see citing works
Liver organoids reproduce alpha-1 antitrypsin deficiency-related liver disease
Gómez‐Mariano, G., Matamala, N., Martínez, S., Justo, I., Marcacuzco, A., Jiménez, C., Monzón, S., Cuesta, I., Garfia, C., Martínez, M. T., et al. (2019) · Hepatology International · doi:10.1007/s12072-019-10007-y · Cited by 75 · see citing works
Recent Applications of Three Dimensional Printing in Cardiovascular Medicine
Gardin, C., Ferroni, L., Latrémouille, C., Chachques, J. C., Mitrečić, D., & Zavan, B. (2020) · Cells · doi:10.3390/cells9030742 · Cited by 75 · see citing works
Production of Multiple Cell‐Laden Microtissue Spheroids with a Biomimetic Hepatic‐Lobule‐Like Structure
Hong, G., Kim, J., Oh, H., Yun, S., Kim, C. M., Jeong, Y., Yun, W., Shim, J., Jang, I., Kim, C., et al. (2021) · Advanced Materials · doi:10.1002/adma.202102624 · Cited by 71 · see citing works
In Vitro Evaluation of Major In Vivo Drug Metabolic Pathways Using Primary Human Hepatocytes and HepaRG Cells in Suspension and a Dynamic Three-Dimensional Bioreactor System
Darnell, M., Ulvestad, M., Ellis, E. C. S., Weidolf, L. O. G., & Andersson, T. B. (2012) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.112.195834 · Cited by 67 · see citing works
A Polyurethane-Gelatin Hybrid Construct for Manufacturing Implantable Bioartificial Livers
Xu, W., Wang, X., Yan, Y., & Zhang, R. (2008) · Journal of Bioactive and Compatible Polymers · doi:10.1177/0883911508095517 · Cited by 65 · see citing works
A Microfabricated Platform for Generating Physiologically-Relevant Hepatocyte Zonation
McCarty, W. J., Usta, O. B., & Yarmush, M. L. (2016) · Scientific Reports · doi:10.1038/srep26868 · Cited by 65 · see citing works
Organoids from adult liver and pancreas: Stem cell biology and biomedical utility
Hindley, C. J., Cordero-Espinoza, L., & Huch, M. (2016) · Developmental Biology · doi:10.1016/j.ydbio.2016.06.039 · Cited by 65 · see citing works
Long‐term liver‐specific functions of hepatocytes in electrospun chitosan nanofiber scaffolds coated with fibronectin
Rajendran, D., Hussain, A., Yip, D., Parekh, A., Shrirao, A. B., & Cho, C. H. (2017) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36072 · Cited by 65 · see citing works
Engineering Liver Microtissues for Disease Modeling and Regenerative Medicine
Huang, D. (., Gibeley, S. B., Xu, C., Xiao, Y., Celik, O., Ginsberg, H. N., & Leong, K. W. (2020) · Advanced Functional Materials · doi:10.1002/adfm.201909553 · Cited by 65 · see citing works
The challenge of developing human 3D organoids into medicines
Vives, J., & Batlle-Morera, L. (2020) · Stem Cell Research & Therapy · doi:10.1186/s13287-020-1586-1 · Cited by 65 · see citing works
Tunable Cross-Linking and Adhesion of Gelatin Hydrogels via Bioorthogonal Click Chemistry
Negrini, N. C., Volponi, A. A., Sharpe, P. T., & Celiz, A. D. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00136 · Cited by 64 · see citing works
Self-citation
Nanobead-on-string composites for tendon tissue engineering
Rinoldi, C., Kijeńska‐Gawrońska, E., Chlanda, A., Choińska, E., Khenoussi, N., Tamayol, A., Khademhosseini, A., & Święszkowski, W. (2018) · Journal of Materials Chemistry B · doi:10.1039/c8tb00246k · Cited by 61 · see citing works
Self-citation
In Vitro Human Liver Model of Nonalcoholic Steatohepatitis by Coculturing Hepatocytes, Endothelial Cells, and Kupffer Cells
Suurmond, C. E., Lasli, S., van den Dolder, F. W., Ung, A., Kim, H., Bandaru, P., Lee, K., Cho, H., Ahadian, S., Ashammakhi, N., et al. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201901379 · Cited by 61 · see citing works
Mimicking Tumor Hypoxia in Non-Small Cell Lung Cancer Employing Three-Dimensional In Vitro Models
Ziółkowska‐Suchanek, I. (2021) · Cells · doi:10.3390/cells10010141 · Cited by 60 · see citing works
A Drug-Induced Hybrid Electrospun Poly-Capro-Lactone: Cell-Derived Extracellular Matrix Scaffold for Liver Tissue Engineering
Grant, R., Hay, D. C., & Callanan, A. (2017) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0419 · Cited by 59 · see citing works
Multicellular Co-Culture in Three-Dimensional Gelatin Methacryloyl Hydrogels for Liver Tissue Engineering
Cui, J., Wang, H., Shi, Q., Sun, T., Huang, Q., & Fukuda, T. (2019) · Molecules · doi:10.3390/molecules24091762 · Cited by 55 · see citing works
Alginate-based tissue-specific bioinks for multi-material 3D-bioprinting of pancreatic islets and blood vessels: A step towards vascularized pancreas grafts
Idaszek, J., Volpi, M., Paradiso, A., Quoc, M. N., Górecka, Ż., Klak, M., Tymicki, G., Berman, A., Wierzbicki, M., Jaworski, S., et al. (2021) · Bioprinting · doi:10.1016/j.bprint.2021.e00163 · Cited by 54 · see citing works
Self-citation
Human hepatocytes loaded in 3D bioprinting generate mini-liver
Zhong, C., Xie, H., Zhou, L., Xu, X., & Zheng, S. (2016) · Hepatobiliary & pancreatic diseases international · doi:10.1016/s1499-3872(16)60119-4 · Cited by 53 · see citing works
A Scalable Approach for the Generation of Human Pluripotent Stem Cell-Derived Hepatic Organoids with Sensitive Hepatotoxicity Features
Sgodda, M., Dai, Z., Zweigerdt, R., Sharma, A. D., Ott, M., & Cantz, T. (2017) · Stem Cells and Development · doi:10.1089/scd.2017.0023 · Cited by 53 · see citing works
Colloidal templating of highly ordered gelatin methacryloyl-based hydrogel platforms for three-dimensional tissue analogues
Lee, B. H., Shirahama, H., Kim, M. H., Lee, J. H., Cho, N.-J., & Tan, L. P. (2017) · NPG Asia Materials · doi:10.1038/am.2017.126 · Cited by 52 · see citing works
Characterization of hepatocyte-based in vitro systems for reliable toxicity testing
Vinken, M., & Hengstler, J. G. (2018) · Archives of Toxicology · doi:10.1007/s00204-018-2297-6 · Cited by 51 · see citing works
A microfluidic patterned model of non-alcoholic fatty liver disease: applications to disease progression and zonation
Bulutoglu, B., Rey-Bedón, C., Kang, Y. B., Mert, S., Yarmush, M. L., & Usta, O. B. (2019) · Lab on a Chip · doi:10.1039/c9lc00354a · Cited by 51 · see citing works
Synthesis and characterization of site selective photo-crosslinkable glycidyl methacrylate functionalized gelatin-based 3D hydrogel scaffold for liver tissue engineering
Sk, M. M., Das, P., Panwar, A., & Tan, L. P. (2021) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.111694 · Cited by 51 · see citing works
Biofabricating murine and human myo‐substitutes for rapid volumetric muscle loss restoration
Costantini, M., Testa, S., Fornetti, E., Fuoco, C., Riera, C. S., Nie, M., Bernardini, S., Rainer, A., Baldi, J., Zoccali, C., et al. (2021) · EMBO Molecular Medicine · doi:10.15252/emmm.202012778 · Cited by 51 · see citing works
Self-citation
Investigation and comparison of new galactosylation methods on PCL/chitosan scaffolds for enhanced liver tissue engineering
Ghahremanzadeh, F., Alihosseini, F., & Semnani, D. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.01.158 · Cited by 50 · see citing works
Bioprinting of Human Liver‐Derived Epithelial Organoids for Toxicity Studies
Bouwmeester, M., Bernal, P. N., Oosterhoff, L. A., van Wolferen, M. E., Lehmann, V., Vermaas, M., Buchholz, M., Peiffer, Q. C., Malda, J., van der Laan, L. J. W., et al. (2021) · Macromolecular Bioscience · doi:10.1002/mabi.202100327 · Cited by 49 · see citing works
Techniques for fabrication and construction of three-dimensional bioceramic scaffolds: Effect on pores size, porosity and compressive strength
Darus, F., Isa, R. M., Mamat, N., & Mariatti, M. (2018) · Ceramics International · doi:10.1016/j.ceramint.2018.07.056 · Cited by 45 · see citing works
Gene Expression Data Based Deep Learning Model for Accurate Prediction of Drug-Induced Liver Injury in Advance
Feng, C., Chen, H., Yuan, X., Sun, M., Chu, K., Liu, H., & Rui, M. (2019) · Journal of Chemical Information and Modeling · doi:10.1021/acs.jcim.9b00143 · Cited by 45 · see citing works
A novel 3D liver organoid system for elucidation of hepatic glucose metabolism
Lü, Y., Zhang, G., Shen, C., Uygun, K., Yarmush, M. L., & Meng, Q. (2011) · Biotechnology and Bioengineering · doi:10.1002/bit.23349 · Cited by 44 · see citing works
Prolongation of liver-specific function for primary hepatocytes maintenance in 3D printed architectures
Kim, Y., Kang, K., Yoon, S., Kim, J. S., Park, S. A., Kim, W. D., Lee, S. B., Ryu, K., Jeong, J., & Choi, D. (2018) · Organogenesis · doi:10.1080/15476278.2018.1423931 · Cited by 42 · see citing works
Recent advances in chemically defined and tunable hydrogel platforms for organoid culture
Agarwal, T., Celikkin, N., Costantini, M., Maiti, T. K., & Makvandi, P. (2021) · Bio-Design and Manufacturing · doi:10.1007/s42242-021-00126-7 · Cited by 42 · see citing works
Self-citation
In vitro liver model using microfabricated scaffolds in a modular bioreactor
Vinci, B., Cavallone, D., Vozzi, G., Mazzei, D., Domenici, C., Brunetto, M. R., & Ahluwalia, A. D. (2009) · Biotechnology Journal · doi:10.1002/biot.200900074 · Cited by 41 · see citing works
BMP antagonists in tissue development and disease
Correns, A., Zimmermann, L. A., Baldock, C., & Sengle, G. (2021) · Matrix Biology Plus · doi:10.1016/j.mbplus.2021.100071 · Cited by 41 · see citing works
An in vitro hepatic zonation model with a continuous oxygen gradient in a microdevice
Sato, A., Kadokura, K., Uchida, H., & Tsukada, K. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.10.017 · Cited by 40 · see citing works
Fibrin Hydrogels for Endothelialized Liver Tissue Engineering with a Predesigned Vascular Network
Wang, X., & Liu, C. (2018) · Polymers · doi:10.3390/polym10101048 · Cited by 40 · see citing works

Flagged first, then by citations

Research works printed but not linked: 227

I. Ziółkowska-Suchanek, Cells, 2021, 10, 141.
I. Kulvinskiene, R. Aldonyte, R. Miksiunas, A. Mobasheri and D. Bironaite, Cell Biol. Transl. Med. Vol. 10 Stem Cells Tissue Regen, 2020, pp. 43-77.
C. Gardin, L. Ferroni, C. Latremouille, J. C. Chachques, D. Mitrečić and B. Zavan, Cells, 2020, 9, 742.
M. Costantini, S. Testa, E. Fornetti, C. Fuoco, C. Sanchez Riera, M. Nie, S. Bernardini, A. Rainer, J. Baldi and C. Zoccali, EMBO Mol. Med., 2021, 13, e12778.
M. Costantini, S. Testa, P. Mozetic, A. Barbetta, C. Fuoco, E. Fornetti, F. Tamiro, S. Bernardini, J. Jaroszewicz, W. Swieszkowski, M. Trombetta, L. Castagnoli, D. Seliktar, P. Garstecki, G. Cesareni, S. Cannata, A. Rainer, C. Gargioli, W. Święszkowski, M. Trombetta, L. Castagnoli, D. Seliktar, P. Garstecki, G. Cesareni, S. Cannata, A. Rainer and C. Gargioli, Biomaterials, 2017, 131, 98-110.
S. Cheemerla and M. Balakrishnan, Clin. Liver Dis., 2021, 17, 365.
R. P. Cunningham and N. Porat-Shliom, Front. Physiol., 2021, 12, 1-17.
J. Ozougwu and J. C. Ozougwu, Int. J. Res. Pharm. Biosci., 2017, 4, 13.
A. S. Serras, J. S. Rodrigues, M. Cipriano, A. V. Rodrigues, N. G. Oliveira and J. P. Miranda, Front. Cell Dev. Biol., 2021, 9, 1-30.
G. K. Michalopoulos and B. Bhushan, Nat. Rev. Gastroenterol. Hepatol., 2021, 18, 40-55.
W. Li, L. Li and L. Hui, Trends Cell Biol., 2020, 30, 329- 338.
H. Lee, S. Chae, J. Y. Kim, W. Han, J. Kim, Y. Choi and D. W. Cho, Biofabrication, 2019, 11(2), 025001.
M. Raasch, E. Fritsche, A. Kurtz, M. Bauer and A. S. Mosig, Adv. Drug Delivery Rev., 2019, 140, 51-67.
X. Ma, J. Liu, W. Zhu, M. Tang, N. Lawrence, C. Yu, M. Gou and S. Chen, Adv. Drug Delivery Rev., 2018, 132, 235-251.
Y. Parmentier, C. Pothier, N. Hewitt, L. Vincent, F. Caradec, J. Liu, F. Lin, M.-M. Trancart, F. Guillet and B. Bouaita, Xenobiotica, 2019, 49, 22-35.
Y. Kim, K. Kang, S. Yoon, J. S. Kim, S. A. Park, W. D. Kim, S. B. Lee, K.-Y. Ryu, J. Jeong and D. Choi, Organogenesis, 2018, 14, 1-12.
E. Milner, M. Ainsworth, M. McDonough, B. Stevens, J. Buehrer, R. Delzell, C. Wilson and J. Barnhill, Med. Drug Discovery, 2020, 8, 100060.
C. M. Smith, C. K. Nolan, M. A. Edwards, J. B. Hatfield, T. W. Stewart, S. S. Ferguson, E. L. Lecluyse and J. Sahi, J. Pharm. Sci., 2012, 101, 3989-4002.
M. Vinken and J. G. Hengstler, Arch. Toxicol., 2018, 92, 2981-2986.
M. R. Mcgill and H. Jaeschke, Biochim. Biophys. Acta, Mol. Basis Dis., 2019, 1865, 1031-1039.
K. Yoshizato and C. Tateno, Expert Opin. Drug Metab. Toxicol., 2013, 9, 1419-1435.
Y. A. Nevzorova, Z. Boyer-Diaz, F. J. Cubero and J. Gracia- Sancho, J. Hepatol., 2020, 73, 423-440.
K. Sakabe, T. Takebe and A. Asai, Clin. Liver Dis., 2020, 15, 3-8.
C. H. Beckwitt, A. M. Clark, S. Wheeler, D. L. Taylor, D. B. Stolz, L. Griffith and A. Wells, Exp. Cell Res., 2018, 363, 15-25.
R. D. Pedde, B. Mirani, A. Navaei, T. Styan, S. Wong, M. Mehrali, A. Thakur, N. K. Mohtaram, A. Bayati, A. Dolatshahi-Pirouz, M. Nikkhah, S. M. Willerth and M. Akbari, Adv. Mater., 2017, 29, 1606061.
J. S. Miller, K. R. Stevens, M. T. Yang, B. M. Baker, D.-H. T. Nguyen, D. M. Cohen, E. Toro, A. A. Chen, P. A. Galie and X. Yu, Nat. Mater., 2012, 11, 768-774.
L. R. Madden, T. V. Nguyen, S. Garcia-Mojica, V. Shah, A. V. Le, A. Peier, R. Visconti, E. M. Parker, S. C. Presnell and D. G. Nguyen, IScience, 2018, 2, 156-167.
J. Sarkar, S. C. Kamble and N. C. Kashikar, Chem, 2021, 3, 164-181.
M. A. Lancaster and J. A. Knoblich, Science, 2014, 345, 1247125.
M. Simian and M. J. Bissell, J. Cell Biol., 2017, 216, 31-40.
M. Hofer and M. P. Lutolf, Nat. Rev. Mater., 2021, 6, 402- 420.
T. Agarwal, N. Celikkin, M. Costantini, T. K. Maiti and P. Makvandi, Bio-Des. Manuf., 2021, 4, 641-674.
V. Magno, A. Meinhardt and C. Werner, Adv. Funct. Mater., 2020, 30, 2000097.
E. A. Aisenbrey and W. L. Murphy, Nat. Rev. Mater., 2020, 5, 539-551.
M. T. Kozlowski, C. J. Crook and H. T. Ku, Commun. Biol., 2021, 4, 1-15.
Y. Wang, H. Liu, M. Zhang, H. Wang, W. Chen and J. Qin, Biomater. Sci., 2020, 8, 5476-5488.
B. J. Klotz, L. A. Oosterhoff, L. Utomo, K. S. Lim, Q. Vallmajo-Martin, H. Clevers, T. B. F. Woodfield, A. J. W. P. Rosenberg, J. Malda and M. Ehrbar, Adv. Healthcare Mater., 2019, 8, 1900979.
M. Saheli, M. Sepantafar, B. Pournasr, Z. Farzaneh, M. Vosough, A. Piryaei and H. Baharvand, J. Cell. Biochem., 2018, 119, 4320-4333.
M. Krüger, L. A. Oosterhoff, M. E. van Wolferen, S. A. Schiele, A. Walther, N. Geijsen, L. De Laporte, L. J. W. van der Laan, L. M. Kock and B. Spee, Adv. Healthcare Mater., 2020, 9, 1901658.
S. Ye, J. W. B. Boeter, M. Mihajlovic, F. G. van Steenbeek, M. E. van Wolferen, L. A. Oosterhoff, A. Marsee, M. Caiazzo, L. J. W. van der Laan and L. C. Penning, Adv. Funct. Mater., 2020, 30, 2000893.
N. Broguiere, L. Isenmann, C. Hirt, T. Ringel, S. Placzek, E. Cavalli, F. Ringnalda, L. Villiger, R. Züllig and R. Lehmann, Adv. Mater., 2018, 30, 1801621.
T. Takebe, K. Sekine, M. Kimura, E. Yoshizawa, S. Ayano, M. Koido, S. Funayama, N. Nakanishi, T. Hisai, T. Kobayashi, T. Kasai, R. Kitada, A. Mori, H. Ayabe, Y. Ejiri, N. Amimoto, Y. Yamazaki, S. Ogawa, M. Ishikawa, Y. Kiyota, Y. Sato, K. Nozawa, S. Okamoto, Y. Ueno and H. Taniguchi, Cell Rep., 2017, 21, 2661-2670.
R. Ouchi, S. Togo, M. Kimura, T. Shinozawa, M. Koido, H. Koike, W. Thompson, R. A. Karns, C. N. Mayhew, P. S. McGrath, H. A. McCauley, R.-R. Zhang, K. Lewis, S. Hakozaki, A. Ferguson, N. Saiki, Y. Yoneyama, I. Takeuchi, Y. Mabuchi, C. Akazawa, H. Y. Yoshikawa, J. M. Wells and T. Takebe, Cell Metab., 2019, 30, 374- 384.
F. Sampaziotis, M. Cardoso de Brito, P. Madrigal, A. Bertero, K. Saeb-Parsy, F. A. C. Soares, E. Schrumpf, E. Melum, T. H. Karlsen, J. A. Bradley, W. T. H. Gelson, S. Davies, A. Baker, A. Kaser, G. J. Alexander, N. R. F. Hannan and L. Vallier, Nat. Biotechnol., 2015, 33, 845-852.
M. Ogawa, S. Ogawa, C. E. Bear, S. Ahmadi, S. Chin, B. Li, M. Grompe, G. Keller, B. M. Kamath and A. Ghanekar, Nat. Biotechnol., 2015, 33, 853-861.
S. Wang, X. Wang, Z. Tan, Y. Su, J. Liu, M. Chang, F. Yan, J. Chen, T. Chen, C. Li, J. Hu and Y. Wang, Cell Res., 2019, 29, 1009-1026.
Y. Guan, D. Xu, P. M. Garfin, U. Ehmer, M. Hurwitz, G. Enns, S. Michie, M. Wu, M. Zheng, T. Nishimura, J. Sage and G. Peltz, JCI Insight, 2017, 2, e94954.
M. Coll, L. Perea, R. Boon, S. B. Leite, J. Vallverdú, I. Mannaerts, A. Smout, A. El Taghdouini, D. Blaya, D. Rodrigo-Torres, I. Graupera, B. Aguilar-Bravo, C. Chesne, M. Najimi, E. Sokal, J. J. Lozano, L. A. van Grunsven, C. M. Verfaillie and P. Sancho-Bru, Cell Stem Cell, 2018, 23, 101-113.
S. S. Ng, K. Saeb-Parsy, S. J. I. Blackford, J. M. Segal, M. P. Serra, M. Horcas-Lopez, S. Mastoridis, W. Jassem, C. W. Frank and N. J. Cho, Biomaterials, 2018, 182, 299-311.
M. N. B. Ramli, Y. S. Lim, C. T. Koe, D. Demircioglu, W. Tng, K. A. U. Gonzales, C. P. Tan, I. Szczerbinska, H. Liang and E. L. Soe, Gastroenterology, 2020, 159, 1471- 1486.
C. J. Hindley, L. Cordero-Espinoza and M. Huch, Dev. Biol., 2016, 420, 251-261.
S. J. Mun, Y.-H. Hong, H.-S. Ahn, J.-S. Ryu, K.-S. Chung and M. J. Son, Int. J. Stem Cells, 2020, 13, 279-286.
J. Vives and L. Batlle-Morera, Stem Cell Res. Ther., 2020, 11, 1-4.
J.-Y. Lee, H.-J. Han, S.-J. Lee, E.-H. Cho, H.-B. Lee, J.-H. Seok, H. S. Lim and W.-C. Son, Int. J. Mol. Sci., 2020, 21, 2982.
N. Dianat, H. Dubois-Pot-Schneider, C. Steichen, C. Desterke, P. Leclerc, A. Raveux, L. Combettes, A. Weber, A. Corlu and A. Dubart-Kupperschmitt, Hepatology, 2014, 60, 700-714.
S. B. Leite, T. Roosens, A. El Taghdouini, I. Mannaerts, A. J. Smout, M. Najimi, E. Sokal, F. Noor, C. Chesne and L. A. van Grunsven, Biomaterials, 2016, 78, 1-10.
B. Artegiani, L. van Voorthuijsen, R. G. H. Lindeboom, D. Seinstra, I. Heo, P. Tapia, C. López-Iglesias, D. Postrach, T. Dayton and R. Oka, Cell Stem Cell, 2019, 24, 927-943.
A. Correns, L.-M. A. Zimmermann, C. Baldock and G. Sengle, Matrix Biol. Plus, 2021, 11, 100071.
M. Urbischek, H. Rannikmae, T. Foets, K. Ravn, M. Hyvönen and M. de la Roche, Sci. Rep., 2019, 9, 1-11.
T. Tao, P. Deng, Y. Wang, X. Zhang, Y. Guo, W. Chen and J. Qin, Adv. Sci., 2022, 9, 2103495.
Y. Lu, G. Zhang, C. Shen, K. Uygun, M. L. Yarmush and Q. Meng, Biotechnol. Bioeng., 2012, 109, 595-604.
C. M. Gamboa, Y. Wang, H. Xu, K. Kalemba, F. E. Wondisford and H. E. Sabaawy, Cells, 2021, 10, 3280.
T. Takebe and J. M. Wells, Science, 2019, 364, 956-959.
M. A. Lancaster, M. Renner, C.-A. Martin, D. Wenzel, L. S. Bicknell, M. E. Hurles, T. Homfray, J. M. Penninger, A. P. Jackson and J. A. Knoblich, Nature, 2013, 501, 373-379.
T. Takebe, K. Sekine, M. Enomura, H. Koike, M. Kimura, T. Ogaeri, R.-R. Zhang, Y. Ueno, Y.-W. Zheng, N. Koike, S. Aoyama, Y. Adachi and H. Taniguchi, Nature, 2013, 499, 481-484.
S. Akbari, G. G. Sevinç, N. Ersoy, O. Basak, K. Kaplan, K. Sevinç, E. Ozel, B. Sengun, E. Enustun, B. Ozcimen, A. Bagriyanik, N. Arslan, T. T. Önder and E. Erdal, Stem Cell Rep., 2019, 13, 627-641.
Y.-Z. Nie, Y.-W. Zheng, K. Miyakawa, S. Murata, R.-R. Zhang, K. Sekine, Y. Ueno, T. Takebe, T. Wakita and A. Ryo, EBioMedicine, 2018, 35, 114-123.
Y. Xia, A. Carpentier, X. Cheng, P. D. Block, Y. Zhao, Z. Zhang, U. Protzer and T. J. Liang, J. Hepatol., 2017, 66, 494-503.
S. Nuciforo, I. Fofana, M. S. Matter, T. Blumer, D. Calabrese, T. Boldanova, S. Piscuoglio, S. Wieland, F. Ringnalda and G. Schwank, Cell Rep., 2018, 24, 1363- 1376.
G. Gómez-Mariano, N. Matamala, S. Martínez, I. Justo, A. Marcacuzco, C. Jimenez, S. Monzón, I. Cuesta, C. Garfia and M. T. Martínez, Hepatol. Int., 2020, 14, 127-137.
L. Broutier, G. Mastrogiovanni, M. M. A. Verstegen, H. E. Francies, L. M. Gavarró, C. R. Bradshaw, G. E. Allen, R. Arnes-Benito, O. Sidorova and M. P. Gaspersz, Nat. Med., 2017, 23, 1424-1435.
G. H. Underhill and S. R. Khetani, Cell. Mol. Gastroenterol. Hepatol., 2018, 5, 426-439.
Y. Jin, J. Kim, J. S. Lee, S. Min, S. Kim, D.-H. Ahn, Y.-G. Kim and S.-W. Cho, Adv. Funct. Mater., 2018, 28, 1801954.
T. Shinozawa, M. Kimura, Y. Cai, N. Saiki, Y. Yoneyama, R. Ouchi, H. Koike, M. Maezawa, R.-R. Zhang and A. Dunn, Gastroenterology, 2021, 160, 831-846.
A. Skardal, M. Devarasetty, C. Rodman, A. Atala and S. Soker, Ann. Biomed. Eng., 2015, 43, 2361-2373.
N. Kaplowitz, Nat. Rev. Drug Discovery, 2005, 4, 489-499.
N. Prior, P. Inacio and M. Huch, Gut, 2019, 68, 2228-2237.
L. Meunier and D. Larrey, Front. Pharmacol., 2019, 10, 1482.
S. J. Mun, J.-S. Ryu, M.-O. Lee, Y. S. Son, S. J. Oh, H.-S. Cho, M.-Y. Son, D.-S. Kim, S. J. Kim, H. J. Yoo, H.-J. Lee, J. Kim, C.-R. Jung, K.-S. Chung and M. J. Son, J. Hepatol., 2019, 71, 970-985.
M. Sgodda, Z. Dai, R. Zweigerdt, A. D. Sharma, M. Ott and T. Cantz, Stem Cells Dev., 2017, 26, 1490-1504.
S. H. Au, M. D. Chamberlain, S. Mahesh, M. V. Sefton and A. R. Wheeler, Lab Chip, 2014, 14, 3290-3299.
T. Nakamura, K. Yoshimoto, Y. Nakayama, Y. Tomita and A. Ichihara, Proc. Natl. Acad. Sci. U. S. A., 1983, 80, 7229- 7233.
S. Nuciforo and M. H. Heim, JHEP Rep., 2021, 3, 100198.
S. N. Boers, J. J. M. van Delden, H. Clevers and A. L. Bredenoord, EMBO Rep., 2016, 17, 938-941.
S. N. Boers and A. L. Bredenoord, Nat. Cell Biol., 2018, 20, 642-645.
N. Sachs, J. de Ligt, O. Kopper, E. Gogola, G. Bounova, F. Weeber, A. V. Balgobind, K. Wind, A. Gracanin and H. Begthel, Cell, 2018, 172, 373-386.
L. R. Volpatti and A. K. Yetisen, Trends Biotechnol., 2014, 32, 347-350.
X. Hou, Y. S. Zhang, G. T. Santiago, M. M. Alvarez, J. Ribas, S. J. Jonas, P. S. Weiss, A. M. Andrews, J. Aizenberg and A. Khademhosseini, Nat. Rev. Mater., 2017, 2, 1-15.
S. Faley, K. Seale, J. Hughey, D. K. Schaffer, S. VanCompernolle, B. McKinney, F. Baudenbacher, D. Unutmaz and J. P. Wikswo, Lab Chip, 2008, 8, 1700- 1712.
D. Patel, A. Haque, Y. Gao and A. Revzin, Integr. Biol., 2015, 7, 815-824.
C.-T. Ho, R.-Z. Lin, R.-J. Chen, C.-K. Chin, S.-E. Gong, H.-Y. Chang, H.-L. Peng, L. Hsu, T.-R. Yew and S.-F. Chang, Lab Chip, 2013, 13, 3578-3587. 101
P. Gissen and I. M. Arias, J. Hepatol., 2015, 63, 1023-1037. 102
F. Yu, R. Deng, W. Hao Tong, L. Huan, N. Chan Way, A. IslamBadhan, C. Iliescu and H. Yu, Sci. Rep., 2017, 7, 14528.
D. Huang, S. B. Gibeley, C. Xu, Y. Xiao, O. Celik, H. N. Ginsberg and K. W. Leong, Adv. Funct. Mater., 2020, 30, 1909553.
C. E. Suurmond, S. Lasli, F. W. van den Dolder, A. Ung, H. Kim, P. Bandaru, K. Lee, H. Cho, S. Ahadian and N. Ashammakhi, Adv. Healthcare Mater., 2019, 8, 1901379. 105
B. Vinci, D. Cavallone, G. Vozzi, D. Mazzei, C. Domenici, M. Brunetto and A. Ahluwalia, Biotechnol. J., 2010, 5, 232- 241.
W. J. McCarty, O. B. Usta and M. L. Yarmush, Sci. Rep., 2016, 6, 1-10. 107
J. Ahn, J.-H. Ahn, S. Yoon, Y. S. Nam, M.-Y. Son and J.-H. Oh, J. Biol. Eng., 2019, 13, 1-15. 109
J.-H. Lee, K.-L. Ho and S.-K. Fan, J. Biomed. Sci., 2019, 26, 88. 110
P. J. Lee, P. J. Hung and L. P. Lee, Biotechnol. Bioeng., 2007, 97, 1340-1346. 111
J. B. Lee, J. S. Park, Y. M. Shin, D. H. Lee, J. Yoon, D. Kim, U. H. Ko, Y. Kim, S. H. Bae and H. Sung, Adv. Funct. Mater., 2019, 29, 1900075. 112
B. Bulutoglu, C. Rey-Bedón, Y. B. A. Kang, S. Mert, M. L. Yarmush and O. B. Usta, Lab Chip, 2019, 19, 3022- 3031.
A. Sato, K. Kadokura, H. Uchida and K. Tsukada, Biochem. Biophys. Res. Commun., 2014, 453, 767-771.
A. Moya, M. Ortega-Ribera, X. Guimerà, E. Sowade, M. Zea, X. Illa, E. Ramon, R. Villa, J. Gracia-Sancho and G. Gabriel, Lab Chip, 2018, 18, 2023-2035. 115
F. T. Lee-Montiel, S. M. George, A. H. Gough, A. D. Sharma, J. Wu, R. DeBiasio, L. A. Vernetti and D. L. Taylor, Exp. Biol. Med., 2017, 242, 1617-1632.
L. A. Vernetti, N. Senutovitch, R. Boltz, R. DeBiasio, T. Ying Shun, A. Gough and D. L. Taylor, Exp. Biol. Med., 2015, 241, 101-114.
A. Skardal, M. Devarasetty, S. Soker and A. R. Hall, Biofabrication, 2015, 7, 31001. 118
Y. Miyamoto, M. Ikeuchi, H. Noguchi, T. Yagi and S. Hayashi, Cell Med., 2015, 8, 47-56. 119
M. Jang, P. Neuzil, T. Volk, A. Manz and A. Kleber, Biomicrofluidics, 2015, 9, 34113. 120
K. M. Bircsak, R. DeBiasio, M. Miedel, A. Alsebahi, R. Reddinger, A. Saleh, T. Shun, L. A. Vernetti and A. Gough, Toxicology, 2021, 450, 152667. 121
M. S. Freag, B. Namgung, M. E. Reyna Fernandez, E. Gherardi, S. Sengupta and H. L. Jang, Hepatol. Commun., 2021, 5, 217-233. 122
J. C. Nawroth, D. B. Petropolis, D. V. Manatakis, T. I. Maulana, G. Burchett, K. Schlünder, A. Witt, A. Shukla, K. Kodella and J. Ronxhi, Cell Rep., 2021, 36, 109393.
H. Lee, W. Han, H. Kim, D.-H. Ha, J. Jang, B. S. Kim and D.-W. Cho, Biomacromolecules, 2017, 18, 1229-1237. 125
S. Lu, F. Cuzzucoli, J. Jiang, L.-G. Liang, Y. Wang, M. Kong, X. Zhao, W. Cui, J. Li and S. Wang, Lab Chip, 2018, 18, 3379-3392. 126
Y.-C. Toh, T. C. Lim, D. Tai, G. Xiao, D. van Noort and H. Yu, Lab Chip, 2009, 9, 2026-2035. 127
A. Carraro, W.-M. Hsu, K. M. Kulig, W. S. Cheung, M. L. Miller, E. J. Weinberg, E. F. Swart, M. Kaazempur- Mofrad, J. T. Borenstein, J. P. Vacanti and C. Neville, Biomed. Microdevices, 2008, 10, 795-805. 128
T. Agarwal, B. Subramanian and T. K. Maiti, ACS Biomater. Sci. Eng., 2019, 5, 4167-4182. 129
S. Mohanty, L. B. Larsen, J. Trifol, P. Szabo, H. V. R. Burri, C. Canali, M. Dufva, J. Emnéus and A. Wolff, Mater. Sci. Eng., C, 2015, 55, 569-578. 130
Y. Liu, L. Zhang, J. Wei, S. Yan, J. Yu and X. Li, J. Mater. Chem. B, 2014, 2, 3029-3040. 131
Y. Pang, Y. Horimoto, S. Sutoko, K. Montagne, M. Shinohara, D. Mathiue, K. Komori, M. Anzai, T. Niino and Y. Sakai, Biofabrication, 2016, 8, 35016.
W. Xu, X. Wang, Y. Yan and R. Zhang, J. Bioact. Compat. Polym., 2008, 23, 409-422. 133
E. S. Place, J. H. George, C. K. Williams and M. M. Stevens, Chem. Soc. Rev., 2009, 38, 1139-1151.
K. R. Stevens, J. S. Miller, B. L. Blakely, C. S. Chen and S. N. Bhatia, J. Biomed. Mater. Res., Part A, 2015, 103, 3331-3338. 135
R. Grant, D. Hay and A. Callanan, Biomed. Phys. Eng. Express, 2018, 4, 65015. 136
N. Contessi Negrini, A. Angelova Volponi, P. T. Sharpe and A. D. Celiz, ACS Biomater. Sci. Eng., 2021, 7, 4330-4346. 137
J. P. Miranda, A. Rodrigues, R. M. Tostões, S. Leite, H. Zimmerman, M. J. T. Carrondo and P. M. Alves, Tissue Eng., Part C, 2010, 16, 1223-1232.
L. Sun, H. Yang, Y. Wang, X. Zhang, B. Jin, F. Xie, Y. Jin, Y. Pang, H. Zhao, X. Lu, X. Sang, H. Zhang, F. Lin, W. Sun, P. Huang and Y. Mao, Front. Oncol., 2020, 10, 878.
X. Wang, Y. Yan, Y. Pan, Z. Xiong, H. Liu, J. Cheng, F. Liu, F. Lin, R. Wu, R. Xhang, Q. Lu, R. Zhang and Q. Lu, Tissue Eng., 2006, 12, 83-91. 140
J. Cui, H. Wang, Q. Shi, T. Sun, Q. Huang and T. Fukuda, Molecules, 2019, 24, 1762. 141
B. H. Lee, H. Shirahama, M. H. Kim, J. H. Lee, N.-J. Cho and L. P. Tan, NPG Asia Mater., 2017, 9, e412. 142
S. Li, Z. Xiong, X. Wang, Y. Yan, H. Liu and R. Zhang, J. Bioact. Compat. Polym., 2009, 24, 249-265. 143
Y. Yan, X. Wang, Z. Xiong, H. Liu, F. Liu, F. Lin, R. Wu, R. Zhang and Q. Lu, J. Bioact. Compat. Polym., 2005, 20, 259-269. 144
N. S. Bhise, V. Manoharan, S. Massa, A. Tamayol, M. Ghaderi, M. Miscuglio, Q. Lang, Y. Shrike Zhang, S. R. R. yo. Shin, G. Calzone, N. Annabi, T. D. Shupe, C. E. Bishop, A. Atala, M. R. Dokmeci and A. Khademhosseini, Biofabrication, 2016, 8, 014101. 146
J. Cui, H. Wang, Q. Shi, P. Ferraro, T. Sun, P. Dario, Q. Huang and T. Fukuda, Acta Biomater., 2020, 113, 328- 338. 147
R. D. Hickey and W. E. Naugler, Hepatology, 2018, 67, 2465-2467. 148
X. Wang and C. Liu, Polymer, 2018, 10, 1048. 149
H. Bruns, U. Kneser, S. Holzhüter, B. Roth, J. Kluth, P. M. Kaufmann, D. Kluth and H. C. Fiegel, Tissue Eng., 2005, 11, 1718-1726. 150
F. J. O'Brien, Mater. Today, 2011, 14, 88-95. 152
N. A. Peppas, J. Z. Hilt, A. Khademhosseini and R. Langer, Adv. Mater., 2006, 18, 1345-1360. 153
D. Mondal, M. Griffith and S. S. Venkatraman, Int. J. Polym. Mater. Polym. Biomater., 2016, 65, 255-265. 154
Y. Zhang, Q.-S. Wang, K. Yan, Y. Qi, G.-F. Wang and Y.-L. Cui, J. Biomed. Mater. Res., Part A, 2016, 104, 1863- 1870. 155
Y. Wang, W. Su, L. Wang, L. Jiang, Y. Liu, L. Hui and J. Qin, Toxicol. Res., 2018, 7, 13-21. 156
S. V. Murphy and A. Atala, Nat. Biotechnol., 2014, 32, 773- 785. 157
J. Malda, J. Visser, F. P. Melchels, T. Jüngst, W. E. Hennink, W. J. A. Dhert, J. Groll and D. W. Hutmacher, Adv. Mater., 2013, 25, 5011-5028.
A. Schwab, R. Levato, M. D'Este, S. Piluso, D. Eglin and J. Malda, Chem. Rev., 2020, 120, 11028-11055. 159
P. L. Lewis and R. N. Shah, Curr. Transplant. Rep., 2016, 3, 100-108. 160
J. W. Lee, Y.-J. Choi, W.-J. Yong, F. Pati, J.-H. Shim, K. S. Kang, I.-H. Kang, J. Park and D.-W. Cho, Biofabrication, 2016, 8, 15007. 161
P. L. Lewis, R. M. Green and R. N. Shah, Acta Biomater., 2018, 69, 63-70. 162
G. Mattei, C. Magliaro, A. Pirone and A. Ahluwalia, Organogenesis, 2018, 14, 129-146. 163
J. M. Sobral, S. G. Caridade, R. A. Sousa, J. F. Mano and R. L. Reis, Acta Biomater., 2011, 7, 1009-1018. 165
F. Darus, R. M. Isa, N. Mamat and M. Jaafar, Ceram. Int., 2018, 44, 18400-18407.
T. Lu, Y. Li and T. Chen, Int. J. Nanomed., 2013, 8, 337. 167
M. Gou, X. Qu, W. Zhu, M. Xiang, J. Yang, K. Zhang, Y. Wei and S. Chen, Nat. Commun., 2014, 5, 1-9. 168
J. Idaszek, M. Volpi, A. Paradiso, M. N. Quoc, Ż. Górecka, M. Klak, G. Tymicki, A. Berman, M. Wierzbicki and S. Jaworski, Bioprinting, 2021, 24, e00163. 169
M. Volpi, A. Paradiso, M. Costantini and W. Świe ¸szkowski, ACS Biomater. Sci. Eng., 2022, 8, 379-405.
W. Peng, D. Unutmaz and I. T. Ozbolat, Trends Biotechnol., 2016, 34, 722-732. 171
L. A. van Grunsven, Adv. Drug Delivery Rev., 2017, 121, 133-146.
A. Faulkner-Jones, C. Fyfe, D.-J. Cornelissen, J. Gardner, J. King, A. Courtney and W. Shu, Biofabrication, 2015, 7, 44102.
C. Zhong, H.-Y. Xie, L. Zhou, X. Xu and S.-S. Zheng, Hepatobiliary Pancreatic Dis. Int., 2016, 15, 512-518. 174
T. Hiller, J. Berg, L. Elomaa, V. Röhrs, I. Ullah, K. Schaar, A.-C. Dietrich, M. A. Al-Zeer, A. Kurtz, A. C. Hocke, S. Hippenstiel, H. Fechner, M. Weinhart and J. Kurreck, Int. J. Mol. Sci., 2018, 19, 3129. 175
N. C. Negrini, N. Celikkin, P. Tarsini, S. Farè and W. Święszkowski, Biofabrication, 2020, 12, 025001.
Q. Mao, Y. Wang, Y. Li, S. Juengpanich, W. Li, M. Chen, J. Yin, J. Fu and X. Cai, Mater. Sci. Eng., C, 2020, 109, 110625. 177
V. M. Lauschke, R. Z. Shafagh, D. F. G. Hendriks and M. Ingelman-Sundberg, Biotechnol. J., 2019, 14, 1800347. 179
D. G. Nguyen, J. Funk, J. B. Robbins, C. Crogan-Grundy, S. C. Presnell, T. Singer and A. B. Roth, PLoS One, 2016, 11, e0158674. 180
E. Goulart, L. C. de Caires-Junior, K. A. Telles-Silva, B. H. S. Araujo, S. A. Rocco, M. Sforca, I. L. de Sousa, G. S. Kobayashi, C. M. Musso, A. F. Assoni, D. Oliveira, E. Caldini, S. Raia, P. I. Lelkes and M. Zatz, Biofabrication, 2019, 12, 15010. 181
H. Kizawa, E. Nagao, M. Shimamura, G. Zhang and H. Torii, Biochem. Biophys. Rep., 2017, 10, 186-191.
L. M. Norona, D. G. Nguyen, D. A. Gerber, S. C. Presnell, M. Mosedale and P. B. Watkins, PLoS One, 2019, 14, e0208958. 183
J. Nie, Q. Gao, J. Fu and Y. He, Adv. Healthcare Mater., 2020, 9, 1901773.
M. A. Heinrich, W. Liu, A. Jimenez, J. Yang, A. Akpek, X. Liu, Q. Pi, X. Mu, N. Hu, R. M. Schiffelers, J. Prakash, J. Xie and Y. S. Zhang, Small, 2019, 15, 1805510.
Y. Wu, Z. Y. Lin, A. C. Wenger, K. C. Tam and X. Tang, Bioprinting, 2018, 9, 1-6. 186
Y. Wu, A. Wenger, H. Golzar and X. Tang, Sci. Rep., 2020, 10, 20648. 187
G. Janani, S. Priya, S. Dey and B. B. Mandal, ACS Appl. Mater. Interfaces, 2022, 14, 10167-10186.
M. Cuvellier, F. Ezan, H. Oliveira, S. Rose, J.-C. Fricain, S. Langouët, V. Legagneux and G. Baffet, Biomaterials, 2021, 269, 120611.
X. Ma, C. Yu, P. Wang, W. Xu, X. Wan, C. S. E. Lai, J. Liu, A. Koroleva-Maharajh and S. Chen, Biomaterials, 2018, 185, 310-321.
X. Ma, X. Qu, W. Zhu, Y.-S. Li, S. Yuan, H. Zhang, J. Liu, P. Wang, C. S. E. Lai, F. Zanella, G.-S. Feng, F. Sheikh, S. Chien and S. Chen, Proc. Natl. Acad. Sci. U. S. A., 2016, 113, 2206-2211. 191
F. Xie, L. Sun, Y. Pang, G. Xu, B. Jin, H. Xu, X. Lu, Y. Xu, S. Du, Y. Wang, S. Feng, X. Sang, S. Zhong, X. Wang, W. Sun, H. Zhao, H. Zhang, H. Yang, P. Huang and Y. Mao, Biomaterials, 2021, 265, 120416. 192
M. Rahmati, D. K. Mills, A. M. Urbanska, M. R. Saeb, J. R. Venugopal, S. Ramakrishna and M. Mozafari, Prog. Mater. Sci., 2021, 117, 100721. 193
C. Rinoldi, E. Kijeńska, A. Chlanda, E. Choinska, N. Khenoussi, A. Tamayol, A. Khademhosseini and W. Swieszkowski, J. Mater. Chem. B, 2018, 6, 3116-3127. 194
I. Fasolino, V. Guarino, M. Marrese, V. Cirillo, M. Vallifuoco, M. L. Tamma, V. Vassallo, A. Bracco, F. Calise and L. Ambrosio, Biomed. Mater., 2017, 13, 15017. 195
R. Grant, J. Hallett, S. Forbes, D. Hay and A. Callanan, Sci. Rep., 2019, 9, 6293. 196
M. Mittal, M. R. Siddiqui, K. Tran, S. P. Reddy and A. B. Malik, Antioxid. Redox Signal., 2014, 20, 1126-1167. 197
R. Grant, D. C. Hay and A. Callanan, Tissue Eng., Part A, 2017, 23, 650-662. 198
Y. Yokoyama, S. Hattori, C. Yoshikawa, Y. Yasuda, H. Koyama, T. Takato and H. Kobayashi, Mater. Lett., 2009, 63, 754-756. 199
S. Khorshidi, A. Solouk, H. Mirzadeh, S. Mazinani, J. M. Lagaron, S. Sharifi and S. Ramakrishna, J. Tissue Eng. Regener Med., 2016, 10, 715-738. 200
J. H. Brown, P. Das, M. D. DiVito, D. Ivancic, L. P. Tan and J. A. Wertheim, Acta Biomater., 2018, 73, 217-227. 201
D. Rajendran, A. Hussain, D. Yip, A. Parekh, A. Shrirao and C. H. Cho, J. Biomed. Mater. Res., Part A, 2017, 105, 2119-2128.
M. M. Sk, P. Das, A. Panwar and L. P. Tan, Mater. Sci. Eng., C, 2021, 123, 111694. 203
F. Ghahremanzadeh, F. Alihosseini and D. Semnani, Int. J. Biol. Macromol., 2021, 174, 278-288.
Y.-H. Chen, Y.-H. Ku, K.-C. Wang, H.-C. Chiang, Y.-P. Hsu, M.-T. Cheng, C.-S. Wang and Y. Wee, Gels, 2022, 8, 149. 205
K. Zeilinger, N. Freyer, G. Damm, D. Seehofer and F. Knöspel, Exp. Biol. Med., 2016, 241, 1684-1698. 206
J. V. Castell, R. Jover, C. P. Martnez-Jimnez and M. J. Gmez-Lechn, Expert Opin. Drug Metab. Toxicol., 2006, 2, 183-212.
H. H. J. Gerets, K. Tilmant, B. Gerin, H. Chanteux, B. O. Depelchin, S. Dhalluin and F. A. Atienzar, Cell Biol. Toxicol., 2012, 28, 69-87.
X. Yang, Y. Meng, Z. Han, F. Ye, L. Wei and C. Zong, Cell Biosci., 2020, 10, 1-18. 209
J. Deng, W. Wei, Z. Chen, B. Lin, W. Zhao, Y. Luo and X. Zhang, Micromachines, 2019, 10, 676. 210
C. Luo, D. Lü, L. Zheng, F. Zhang, X. Zhang, S. Lü, C. Zhang, X. Jia, X. Shu and P. Li, Biomater. Sci., 2021, 9, 3776-3790. 211
F. Jacob, R. D. Salinas, D. Y. Zhang, P. T. T. Nguyen, J. G. Schnoll, S. Z. H. Wong, R. Thokala, S. Sheikh, D. Saxena and S. Prokop, Cell, 2020, 180, 188-204.
A. J. Engler, S. Sen, H. L. Sweeney and D. E. Discher, Cell, 2006, 126, 677-689.
A. Treyer and A. Müsch, Compr. Physiol., 2013, 3, 243. 214
C. Jensen and Y. Teng, Front. Mol. Biosci., 2020, 7, 33.
A. S. Serras, J. S. Rodrigues, M. Cipriano, A. V. Rodrigues, N. G. Oliveira and J. P. Miranda, Front. Cell Dev. Biol., 2021, 9, 626805.

200 of 227 shown

Sorted by citations

From Organ-on-a-Chip to Human-on-a-Chip: A Review of Research Progress and Latest Applications
Huang, Y., Liu, T., Huang, Q., & Wang, Y. (2024) · ACS Sensors · doi:10.1021/acssensors.4c00004 · Cited by 93 · see citing works
3D bioprinting of liver models: A systematic scoping review of methods, bioinks, and reporting quality
Ali, A. S. M., Wu, D., Bannach‐Brown, A., Dhamrait, D., Berg, J., Tolksdorf, B., Lichtenstein, D., Dressler, C., Braeuning, A., Kurreck, J., et al. (2024) · Materials Today Bio · doi:10.1016/j.mtbio.2024.100991 · Cited by 41 · see citing works
Designer cellular spheroids with DNA origami for drug screening
Wei, J., Sun, Y., Wang, H., Zhu, T., Li, L., Zhou, Y., Liu, Q., Dai, Z., Li, W., Yang, T., et al. (2024) · Science Advances · doi:10.1126/sciadv.ado9880 · Cited by 40 · see citing works
3D Bioprinting of an Endothelialized Liver Lobule-like Construct as a Tumor-Scale Drug Screening Platform
Fan, Z., Wei, X., Chen, K., Wang, L., & Xu, M. (2023) · Micromachines · doi:10.3390/mi14040878 · Cited by 38 · see citing works
Genome-wide pan-GPCR cell libraries accelerate drug discovery
Yang, H., Wang, Y., Liu, W., He, T., Liao, J., Qian, Z., Zhao, J., Cong, Z., Sun, D., Liu, Z., et al. (2024) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2024.06.023 · Cited by 37 · see citing works
Microfluidic systems for modeling digestive cancer: a review of recent progress
Razavi, Z., Soltani, M., Pazoki-Toroudi, H., & Dabagh, M. (2024) · Biomedical Physics & Engineering Express · doi:10.1088/2057-1976/ad6f15 · Cited by 24 · see citing works
Vascularized tissue on mesh-assisted platform (VT-MAP): a novel approach for diverse organoid size culture and tailored cancer drug response analysis
Lee, J., Jung, S., Hong, H. K., Jo, H., Rhee, S., Jeong, Y., Ko, J., Cho, Y. B., & Jeon, N. L. (2024) · Lab on a Chip · doi:10.1039/d3lc01055d · Cited by 18 · see citing works
Decellularized plant-derived vasculature-on-a-chip interacting with breast cancer spheroids to evaluate a dual-drug therapy
Filiz, Y., Arslan, Y., Duran, E., Saglam‐Metiner, P., Horozoglu, S., Paradiso, A., Martinez, D. C., Takanlou, M. S., Heljak, M. K., Jaroszewicz, J., et al. (2023) · Applied Materials Today · doi:10.1016/j.apmt.2023.102015 · Cited by 16 · see citing works
Self-citation
Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chip
Byeon, J. H., Jung, D. J., Han, H.-J., Son, W.-C., & Jeong, G. S. (2024) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2024.1452485 · Cited by 12 · see citing works
“Physiological relevant in vitro models provide a promising platform for modelling the physiochemical conditions of the hepatic microenvironment (Janani and Mandal, 2021; Kostrzewski et al., 2021; Paradiso et al., 2023).”
The impact of matrix stiffness on hepatic cell function, liver fibrosis, and hepatocellular carcinoma—Based on quantitative data
Min, K., Karuppannan, S. K., & Tae, G. (2024) · Biophysics Reviews · doi:10.1063/5.0197875 · Cited by 10 · see citing works
Current Treatment Regimens and Promising Molecular Therapies for Chronic Hepatobiliary Diseases
Durazzo, M., Ferro, A., Navarro‐Tableros, V., Gaido, A., Fornengo, P., Altruda, F., Romagnoli, R., Moestrup, S. K., Calvo, P. L., & Fagoonee, S. (2025) · Biomolecules · doi:10.3390/biom15010121 · Cited by 11 · see citing works
Three-tier framework for high-throughput biofabrication: Integrating 3D bioprinting, assistive platforms, and translational opportunities
Singh, Y. P., Moses, J. C., Kim, M.-H., Gupta, D., Pal, V., Deniz, I. D., Gerhard, E. M., & Ozbolat, I. T. (2025) · Bioactive Materials · doi:10.1016/j.bioactmat.2025.11.024 · Cited by 11 · see citing works
Building in vitro models for mechanistic understanding of liver regeneration in chronic liver diseases
Karnawat, K., Parthasarathy, R., Sakhrie, M., Karthik, H., Krishna, K. V., & Balachander, G. M. (2024) · Journal of Materials Chemistry B · doi:10.1039/d4tb00738g · Cited by 8 · see citing works
Evaluating the synergistic use of advanced liver models and AI for the prediction of drug-induced liver injury
Zhou, Y., Zhong, Y., & Lauschke, V. M. (2025) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1080/17425255.2025.2461484 · Cited by 8 · see citing works
In vitro liver models for toxicological research
Fukunaga, I., & Takebe, T. (2025) · Drug Metabolism and Pharmacokinetics · doi:10.1016/j.dmpk.2025.101478 · Cited by 7 · see citing works
Harnessing Next‐Generation 3D Cancer Models to Elucidate Tumor‐Microbiome Crosstalk
Buzhor, M. G., Longobardi, G., Kandli, O., Krinsky, A., Avramoff, O., Katyal, A., Salomon, K., Miari, A., Venkert, D., Barnatan, T. T., et al. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202503198 · Cited by 7 · see citing works
Generation of Patterned Cocultures in 2D and 3D: State of the Art
Joshi, A., & Singh, N. (2023) · ACS Omega · doi:10.1021/acsomega.3c02713 · Cited by 8 · see citing works
Enhanced liver cancer cellular response to a drug on a 3D nanostructured matrix of decellularized eggplant leaves
Ahmadian, M., Hosseini, S., Alipour, A., Kazemi, J., Farrokhi, N., Jahanfar, M., Homaeigohar, S., & Shahsavarani, H. (2024) · Materials Today Communications · doi:10.1016/j.mtcomm.2024.109318 · Cited by 5 · see citing works
Adenine base editing rescues disrupted BCKDH function and reduces BCAAs toxic accumulation in maple syrup urine disease patient iPSC-hepatic organoids
Zhang, H., Wan, Z., Li, X., Wang, B., Guan, J., Li, Y., Jin, X., Ma, X., & Liu, G. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04630-w · Cited by 4 · see citing works
Exploring animal models and in vitro models for liver diseases: a review
Bhatt, N. S., Prajapati, A., & Shah, G. B. (2025) · Egyptian Liver Journal · doi:10.1186/s43066-025-00459-3 · Cited by 4 · see citing works
Transforming drug discovery through the fusion of AI-driven analysis and protein micropatterning
Roach, P. (2025) · Expert Opinion on Drug Discovery · doi:10.1080/17460441.2025.2567300 · Cited by 3 · see citing works
Development of an enhanced liver scaffold recellularization using fibronectin
Afrin, S., Yadav, U., Yadav, C. J., Kamel, J., Lee, J., & Park, K. (2025) · Journal of Biomaterials Applications · doi:10.1177/08853282251350315 · Cited by 2 · see citing works
Microrheology-on-a-chip: A microfluidic pathway to measure the viscoelasticity of biogels
Deka, R., Das, S., Kakati, N., Agrawal, S. S., Dubey, S., De, S., Deshmukh, O. S., & Bandyopadhyay, D. (2025) · Physics of Fluids · doi:10.1063/5.0286916 · Cited by 1 · see citing works
Development of a primary hepatocyte-based preclinical spheroid model system for the analysis of Phase I and Phase II enzyme activity in vitro
Arai, K., Fukuhara-Obonai, K., Ishimaru, S., Kojima, N., Mir, T. A., Kitsuka, T., & Hosaka, M. (2026) · Engineered Regeneration · doi:10.1016/j.engreg.2026.01.001 · Cited by 1 · see citing works
Microfabricated Liver‐on‐a‐Chip with Membrane‐Free Endothelial–Hepatic Interface for Long‐Term Culture and Drug‐Induced Liver Injury Modeling
Sati, P., Ali, R., Verma, M., Tiwari, N., Verma, S. K., & Sivakumar, S. (2026) · Advanced Healthcare Materials · doi:10.1002/adhm.202503454 · Cited by 2 · see citing works
Roles of the Complement System in Peripheral Nerve Injury and Repair
Azar, C., Pan, K., Jain, P., Sánchez‐López, E., & Shah, S. B. (2026) · Bioengineering · doi:10.3390/bioengineering13020251 · Cited by 1 · see citing works
Designer Cellular Spheroids with DNA Origami for Drug ScreeningPreprint
Pei, H., Wei, J., Sun, Y., Wang, H., Zhu, T., Li, L., Zhou, Y., Liu, Q., Dai, Z., Li, W., et al. (2023) · Research Square · doi:10.21203/rs.3.rs-3555194/v1
Bavachinin causes cholestasis by down-regulating BAAT expression and disrupting glycocholic acid synthesis in human liver organoids
Wang, X., Su, Y., Chen, X., Liu, L., Zhao, X., & Jia, J. (2025) · Food and Chemical Toxicology · doi:10.1016/j.fct.2025.115438
A microfluidic liver-like model for antiepileptic drugs hepatotoxicity evaluation
Moradi, E., Heirani‐Tabasi, A., Ghiass, M. A., & Solati‐Hashjin, M. (2025) · Biomedical Physics & Engineering Express · doi:10.1088/2057-1976/ae1506 · Cited by 1 · see citing works
Physicochemical characterization, lipid-modulating effects, and proteomic insights of Malaysian Sacha Inchi (Plukenetia volubilis L.) seed oil in HepG2 cells
Fardush, M., Fauzi, N. M., Yan, H. W., Nurdin, A., Jantan, I., Muhammad, N. A. B. N., Hazim, S. N., Mustafa, S. H. B., & Feguro, K. (2025) · Food Bioscience · doi:10.1016/j.fbio.2025.107891
Bioprinted spheroid-based liver equivalents: functionality and applicability in assessing pharmacodymanics
Ivleva, A., Bikmulina, P., Brumberg, V. A., Fayzullin, A. L., Heydari, Z., Mozherov, A. M., Krylov, D. P., Kuznetsova, D. S., Pereira, F. D. A. D. S., Solovieva, A. B., et al. (2026) · Bioprinting · doi:10.1016/j.bprint.2026.e00467
Renal and hepatic disorders: Early indicators and diagnostic tools
Goel, F., Bhardwaj, V., Garg, V. K., Kumar, A., Singh, P., & Yadav, D. K. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-33263-0.00010-3
Engineering ethical test systems: Tissue-engineered and in vitro alternatives to in vivo experiments
Sanattalab, E., Zourazema, S., Zendeh, Z., Kaya, K., Çeçen, B., & Kozacı, L. D. (2026) · NAM journal. · doi:10.1016/j.namjnl.2026.100094 · Cited by 1 · see citing works
Tripterygium Glycosides Extract-Induced Hepatic Cholestasis: A Mechanistic Study Using a Microfluidic Liver-on-a-Chip System
Yang, Y., Zhang, Y., Yang, Y., Xia, B., Zhang, H., Zhang, G., Gong, P., Qi, Y., Wu, Z., Li, C., et al. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27094154
Comparative Analysis of Classical and Emerging Experimental Animal Models of Liver Injury: A Comprehensive Review
Salman, I. N., Jabbar, R. K., Emad, I., Hassan, Z. A. A., Harbi, A. M., Khudhair, A. Y., Hussein, M. A. M., Hamid, A. M., Amir, M. A. A., Ali, M. W., et al. (2026) · Journal of Applied Toxicology · doi:10.1002/jat.70268
Three Dimensional Biomimetic Liver Models to Reconstitute Metabolic Dysfunction-Associated Steatohepatitis (MASH) In Vitro: Hope and Reality
Jalise, S. Z., Habibi, S., Alizadeh, F., Heidari, F., & Fath‐Bayati, L. (2026) · Journal of Clinical and Experimental Hepatology · doi:10.1016/j.jceh.2026.103599
Juglone Attenuates CCl₄‐Induced Hepatic Injury in Mice With Associated Modulation of Oxidative Stress and Inflammasome‐Related mRNA Expression
Sohail, A., Sufyan, R., Asim, M., Khan, M., Altaf, A., Haq, M. E. U., Arshad, M., Khalil, A. A. K., & Ullah, I. (2026) · BioMed Research International · doi:10.1155/bmri/8905392
Advancing Organ-on-a-Chip Technologies: A Review on the Multi-Organ Approach for Precision Disease Modeling and Drug Screening
Dhal, A., Aviña, A. E., Peng, P.-W., & Yang, T. (2026) · ACS Applied Engineering Materials · doi:10.1021/acsaenm.6c00402
When safe is not safe together: Co-exposure to individually sub-cytotoxic compounds alters functional integrity of HepG2-derived liver 3D spheroids
Ghasemzadeh‐Hasankolaei, M., McCormick, S., Magne, L., Vrana, N. E., & Ghaemmaghami, A. M. (2026) · Toxicology · doi:10.1016/j.tox.2026.154575
Liver‐on‐Chip Models as Miniaturized Platforms for In Vitro Hepatotoxicity Testing
Sahoo, S., & Bishi, D. K. (2026) · Journal of Applied Toxicology · doi:10.1002/jat.70435

In Vitro Models of the Liver: Disease Modeling, Drug Discovery and Clinical Applications
Collins, S. D., Yuen, G., Tu, T., Budzinska, M. A., Spring, K. J., Bryant, K., & Shackel, N. A. (2019) · Codon Publications eBooks · doi:10.15586/hepatocellularcarcinoma.2019.ch3 · Cited by 41 · see citing works
Advances in liver organoids: model systems for liver disease
Lee, J., Mun, S. J., Shin, Y., Lee, S., & Son, M. J. (2022) · Archives of Pharmacal Research · doi:10.1007/s12272-022-01390-6 · Cited by 21 · see citing works
Advances in Liver Organoid Technology: A Paradigm Shift in Hepatic Research and Therapeutics
Pentela, B., Mazumder, R., & Mazumder, A. (2026) · Current Pharmaceutical Design · doi:10.2174/0113816128440756260210044332
Emerging trends in modeling human liver disease in vitro
Underhill, G. H., & Khetani, S. R. (2019) · APL Bioengineering · doi:10.1063/1.5119090 · Cited by 26 · see citing works
Liver organoid platforms for disease modeling and drug testing
Cho, S. (2022) · Annals of Hepato-Biliary-Pancreatic Surgery · doi:10.14701/ahbps.2022s1.br-1-4

Authors: Paradiso, A., Volpi, M., Rinoldi, C., Celikkin, N., Contessi Negrini, N., Bilgen, M., Dallera, G., Pierini, F., Costantini, M., Święszkowski, W., & Farè, S.
Affiliations: Warsaw University of Technology (PL)
Institute of Fundamental Technological Research (PL)
Institute of Physical Chemistry (PL)
Department of Chemistry, Materials and Chemical Engineering “G. Natta”, Politecnico di Milano, Milan, Italy
Politecnico di Milano (IT)
National Interuniversity Consortium of Materials Science and Technology (IT)
Corresponding author: Święszkowski, Wojciech
Farè, Silvia
ORCID: Paradiso, Alessia
Volpi, Marina
Rinoldi, Chiara
Celikkin, Nehar
Contessi Negrini, Nicola
Bilgen, Muge
Pierini, Filippo
Costantini, Marco
Święszkowski, Wojciech
Farè, Silvia
Venue: Biomaterials Science
Publisher: Royal Society of Chemistry (RSC)
Published: 2023
Volume: 11
Issue: 9
Pages: 2988-3015
ISSN: 2047-4830 (ISSN-L)
2047-4849
DOI: 10.1039/d1bm01872h
OpenAlex: W4310705293
Cited by: 40
References: 230
Citations by year: 2026: 13 · 2025: 13 · 2024: 10 · 2023: 4
FWCI: 0.60
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
creativecommons.org/licenses/by/3.0/ (vor)
Locations: Biomaterials Science (publishedVersion, cc-by)
PubMed (publishedVersion)
CeON Repository (Centre for Evaluation in Education and Science) (submittedVersion, cc-by)
Virtual Community of Pathological Anatomy (University of Castilla La Mancha) (submittedVersion, cc-by)
Funders: Politechnika Warszawska
European Regional Development Fund
Ministerstwo Edukacji i Nauki
Fundacja na rzecz Nauki Polskiej (POIR.04.04.00-00-5ED7/18-00)
Narodowe Centrum Nauki (2018/31/D/ST8/03647, 2020/37/N/ST5/03272)
Narodowe Centrum Badań i Rozwoju (PL-TW/VI/3/2019, STRATEGMED3/305813/2/NCBR/2017)
Topics: Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords: in vitro liver models, drug screening, alternatives to animal testing, organoids, liver-on-a-chip, engineered scaffolds, patient-tailored therapy, liver disease modeling
MeSH terms: Animals, Drug Evaluation, Preclinical, Humans, Organoids, Liver Failure, Acute, Models, Animal

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

In vitro functional models for human liver diseases and drug screening: beyond animal testing

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Self-check

Cites

Cited by

Related

Record

About these checks