Bioengineering Approaches for the Advanced Organoid Research
Open accessAbstract
Recent advances in 3D cell culture technology have enabled scientists to generate stem cell derived organoids that recapitulate the structural and functional characteristics of native organs. Current organoid technologies have been striding toward identifying the essential factors for controlling the processes involved in organoid development, including physical cues and biochemical signaling. There is a growing demand for engineering dynamic niches characterized by conditions that resemble in vivo organogenesis to generate reproducible and reliable organoids for various applications. Innovative biomaterial‐based and advanced engineering‐based approaches have been incorporated into conventional organoid culture methods to facilitate the development of organoid research. The recent advances in organoid engineering, including extracellular matrices and genetic modulation, are comprehensively summarized to pinpoint the parameters critical for organ‐specific patterning. Moreover, perspective trends in developing tunable organoids in response to exogenous and endogenous cues are discussed for next‐generation developmental studies, disease modeling, and therapeutics.
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Organoid cultures often lack stromal, vascular, and immunological components.
“However, organoid cultures exhibit significant heterogeneity, variable complexity in cellular composition, can undergo poorly-controlled morphogenesis in self-assembly process, and often lack stromal, vascular and immunological components4,13.”
From Organoids (2022) · cites this paper as [4]
The ‘Limitations of the Conventional Methods for Organoid Productions’ section explicitly identifies an absence of vascular systems in current cultures. ‘Concluding Remarks and Future Perspectives’ describes difficulties incorporating connective tissues, which partly supports the stromal point, but the passages do not establish that organoid cultures often lack immunological components. Page numbers are unavailable in our copy. (LLM, conf=0.85)
Experimental — accuracy not yet measuredOrganoid cultures can undergo poorly controlled morphogenesis during self-assembly.
“However, organoid cultures exhibit significant heterogeneity, variable complexity in cellular composition, can undergo poorly-controlled morphogenesis in self-assembly process, and often lack stromal, vascular and immunological components4,13.”
From Organoids (2022) · cites this paper as [4]
Organoid cultures vary in the complexity of their cellular composition.
“However, organoid cultures exhibit significant heterogeneity, variable complexity in cellular composition, can undergo poorly-controlled morphogenesis in self-assembly process, and often lack stromal, vascular and immunological components4,13.”
From Organoids (2022) · cites this paper as [4]
Organoid cultures show substantial heterogeneity.
“However, organoid cultures exhibit significant heterogeneity, variable complexity in cellular composition, can undergo poorly-controlled morphogenesis in self-assembly process, and often lack stromal, vascular and immunological components4,13.”
From Organoids (2022) · cites this paper as [4]
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- Authors
- Yi, S. A., Zhang, Y., Rathnam, C., Pongkulapa, T., & Lee, K.
- Affiliations
- Rutgers, The State University of New Jersey (US)
- Corresponding author
- Lee, Ki‐Bum
- Venue
- Advanced Materials
- Publisher
- Wiley
- Published
- 2021-09-24
- Volume
- 33
- Issue
- 45
- Pages
- e2007949-e2007949
- ISSN
- 0935-9648 (ISSN-L)
- 1521-4095
- PMC
- PMC8682947
- OpenAlex
- W3199075493
- Cited by
- 218
- References
- 235
- Citations by year
- 2026: 50 · 2025: 76 · 2024: 53 · 2023: 26 · 2022: 12 · 2021: 1
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Advanced Materials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Deafness and Other Communication Disorders (R01DC016612)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R21AR071101)
- National Institute of Biomedical Imaging and Bioengineering (T32EB005583)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- organoids, organoid engineering, 3D cell culture, stem cell-derived organoids, extracellular matrix, gene modulation, organogenesis, disease modeling, biomaterials
- MeSH terms
- Cell Culture Techniques, Three Dimensional, Biocompatible Materials, Biomedical Engineering, Extracellular Matrix, Genetic Engineering, Humans, Neoplasms, Organoids, Stem Cells, Hydrogels
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