Neuroprotective effect of trans-N-caffeoyltyramine from Lycium chinense against H2O2 induced cytotoxicity in PC12 cells by attenuating oxidative stress
Abstract
Natural products play a critical role in the promotion of good health as regards the prevention and management of oxidative stress related and neurodegenerative disorders. Oxidative stress has been implicated in several apoptotic pathways associated with cell damages in neuronal disorders. The aim of this study was to investigate the antioxidant effect of trans-N-caffeoyltyramine (TNC) isolated from Cortex lycii against hydrogen peroxide (H 2 O 2) induced cell damages in PC12 cells as well as its mechanism of action. The results obtained indicated that pretreatment with TNC before the exposure of cells to H 2 O 2 toxicity lead to a significant increase in the cell viability and the antioxidant enzyme activities catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) and reduced the level of malondialdehyde (MDA). Furthermore, TNC attenuated the influx of Ca 2+, ROS formation and restored the impaired mitochondria membrane potential (MMP). Thus TNC may be used as an alternative therapy for the prevention and treatment of neuronal disorders elicited by oxidative stress.
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In PC12 cells, control apoptotic rate was 2.7%, H2O2 control 23.8%, and pretreatment with 20 μM and 40 μM N-trans-caffeoyltyramine gave apoptotic rates of 11.7% and 7.3%, suggesting a neuroprotective function.
“Where control PC12 cells had an apoptotic rate of 2.7%, the H2O2 control had a rate of 23.8%. Cells pretreated with 20 μM and 40 μM of N-trans-caffeoyltyramine had an apoptotic rate of 11.7% and 7.3%, respectively, suggesting a neuroprotective function.”
N-trans-caffeoyltyramine from Lycium chinense protects PC12 cells from H2O2-induced apoptotic cell death.
“N-trans-caffeoyltyramine extracted from Lycium chinense was reportedly effective in protecting PC12 cells from apoptotic cell death induced by H2O2 [75].”
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- Authors
- Olatunji, O. J., Chen, H., & Zhou, Y.
- Affiliations
- Prince of Songkla University (TH)
- Jiangsu University (CN)
- Chinese Academy of Sciences (CN)
- Corresponding author
- Zhou, Yifeng
- Venue
- Biomedicine & Pharmacotherapy
- Publisher
- Elsevier BV
- Published
- 2017-09
- Volume
- 93
- Pages
- 895-902
- ISSN
- 0753-3322 (ISSN-L)
- OpenAlex
- W2736089300
- Cited by
- 38
- References
- 38
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 3 · 2023: 2 · 2022: 8 · 2021: 6 · 2020: 5 · 2019: 3 · 2018: 2 · 2017: 2
- FWCI
- 4.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biomedicine & Pharmacotherapy (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Public Science and Technology Research Funds Project of Oceans (201505023)
- Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm (BM2014047)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- Lycium chinense, neuroprotection, oxidative stress, hydrogen peroxide cytotoxicity, PC12 cells, antioxidant enzymes, catalase, superoxide dismutase, glutathione, malondialdehyde, reactive oxygen species, mitochondrial membrane potential, calcium influx, neurodegenerative diseases, natural products
- MeSH terms
- Animals, Antioxidants, Caffeic Acids, Catalase, Cell Survival, Glutathione, Glutathione Peroxidase, Hydrogen Peroxide, Malondialdehyde, Neurons, Plant Extracts, Superoxide Dismutase, Tyramine, PC12 Cells, Apoptosis, Reactive Oxygen Species, Oxidative Stress, Neuroprotective Agents, Lycium, Cell Line, Tumor, Rats, Membrane Potential, Mitochondrial
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