Separation and fractionation of exopolysaccharides from Porphyridium cruentum
Abstract
In this work the extraction of EPSs from culture media of Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) and membrane separation techniques has been studied. Diafiltration (DF) using a membrane with a 300 kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6 g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers.
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Filtration techniques are well suited for initial extraction and purification at industrial scale because they can be automatized and handle large volumes.
“Filtration techniques seem well suited for initial extraction and purification steps at industrial scale as they can be automatized and allow the separation of large volumes of solutions”
From Polysaccharides from macroalgae: Recent advances, innovative technologies and challenges in extraction and purification (2017) · cites this paper as Patel et al., 2013
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- Authors
- Patel, A. K., Laroche, C., Marcati, A., Ursu, A. V., Jubeau, S., Marchal, L., Petit, E., Djelveh, G., & Michaud, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Biologie des Plantes et Innovation (FR)
- Corresponding author
- Michaud, Philippe
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2013-10
- Volume
- 145
- Pages
- 345-350
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W1970323354
- Cited by
- 174
- References
- 28
- Citations by year
- 2026: 13 · 2025: 15 · 2024: 8 · 2023: 14 · 2022: 19 · 2021: 23 · 2020: 21 · 2019: 12 · 2018: 16 · 2017: 8 · 2016: 12 · 2015: 5 · 2014: 5 · 2013: 3
- FWCI
- 6.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Oskar-Bordeaux (Universite de Bordeaux) (submittedVersion)
- Funders
- Agence Nationale de la Recherche
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- exopolysaccharides, Porphyridium cruentum, red microalgae, diafiltration, membrane separation, solvent precipitation, dialysis, extraction, rheological properties, shear thinning, critical overlap concentration, monosaccharide composition, polysaccharide fractionation
- MeSH terms
- Ethanol, Methanol, Culture Media, Dialysis, Filtration, Hydrolysis, Monosaccharides, Polysaccharides, Bacterial, Chemical Precipitation, Rheology, 2-Propanol, Porphyridium
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