Cited by 174Polysaccharides Composition and ApplicationsAlgal biology and biofuel productionSeaweed-derived Bioactive Compounds

Separation and fractionation of exopolysaccharides from Porphyridium cruentum

Patel, Anil Kumar, Laroche, Celine, Marcati, Alain, Ursu, Alina Violeta, Jubeau, Sébastien, Marchal, Luc, Petit, Emmanuel, Djelveh, Gholamreza +1 more·2013·Bioresource Technology

Abstract

In this work the extraction of EPSs from culture media of Porphyridium cruentum, by dialysis, solvent-precipitation with 3 polar alcohols (methanol, ethanol and isopropanol) and membrane separation techniques has been studied. Diafiltration (DF) using a membrane with a 300 kDa molecular weight cut off was the most efficient technique compared to solvent-extraction and dialysis methods. After extraction, EPS fraction was characterized in terms of rheological properties and biochemical content. The product exhibited shear thinning behavior and a critical overlap concentration equal to 0.6 g/L. The monosaccharide composition was investigated after acidic hydrolysis. Xylose, galactose, glucose and glucuronic acid were identified as the main constitutive monomers.

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Crossref · 10.1016/j.biortech.2012.12.038

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Authors
Patel, A. K., Laroche, C., Marcati, A., Ursu, A. V., Jubeau, S., Marchal, L., Petit, E., Djelveh, G., & Michaud, P.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Biologie des Plantes et Innovation (FR)
Corresponding author
Michaud, Philippe
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2013-10
Volume
145
Pages
345-350
ISSN
0960-8524 (ISSN-L)
OpenAlex
W1970323354
Cited by
174
References
28
Citations by year
2026: 13 · 2025: 15 · 2024: 8 · 2023: 14 · 2022: 19 · 2021: 23 · 2020: 21 · 2019: 12 · 2018: 16 · 2017: 8 · 2016: 12 · 2015: 5 · 2014: 5 · 2013: 3
FWCI
6.38
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • Oskar-Bordeaux (Universite de Bordeaux) (submittedVersion)
Funders
Agence Nationale de la Recherche
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
Keywords
exopolysaccharides, Porphyridium cruentum, red microalgae, diafiltration, membrane separation, solvent precipitation, dialysis, extraction, rheological properties, shear thinning, critical overlap concentration, monosaccharide composition, polysaccharide fractionation
MeSH terms
Ethanol, Methanol, Culture Media, Dialysis, Filtration, Hydrolysis, Monosaccharides, Polysaccharides, Bacterial, Chemical Precipitation, Rheology, 2-Propanol, Porphyridium

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