Calcium response in osteocytic networks under steady and oscillatory fluid flow

Lu, X. Lucas; Huo, Bo; Park, Miri; Guo, X. Edward

doi:10.1016/j.bone.2012.05.021

Cited by 105Bone Metabolism and DiseasesCellular Mechanics and InteractionsBone health and osteoporosis research

Calcium response in osteocytic networks under steady and oscillatory fluid flow

Open access

Lu, X. Lucas, Huo, Bo, Park, Miri, Guo, X. Edward·2012·Bone

DOI 10.1016/j.bone.2012.05.021PMC PMC3412915

Open this paper in CoChat — free account Open PDF

Abstract

The fluid flow in the lacunar-canalicular system of bone is an essential mechanical stimulation on the osteocyte networks. Due to the complexity of human physical activities, the fluid shear stress on osteocyte bodies and processes consists of both steady and oscillatory components. In this study, we investigated and compared the intracellular calcium ([Ca(2+)](i)) responses of osteocytic networks under steady and oscillatory fluid flows. An in vitro osteocytic network was built with MLO-Y4 osteocyte-like cells using micro-patterning techniques to simulate the in vivo orderly organization of osteocyte networks. Sinusoidal oscillating fluid flow or unidirectional steady flow was applied on the cell surface with 2Pa peak shear stress. It was found that the osteocytic networks were significantly more responsive to steady flow than to oscillatory flow. The osteocytes can release more calcium peaks with higher magnitudes at a faster speed under steady flow stimulation. The [Ca(2+)](i) signaling transients under the steady and oscillatory flows have significantly different spatiotemporal characters, but a similar responsive percentage of cells. Further signaling pathway studies using inhibitors showed that endoplasmic reticulum (ER) calcium store, extracellular calcium source, ATP, PGE(2) and NO related pathways play similar roles in the [Ca(2+)](i) signaling of osteocytes under either steady or oscillating flow. The spatiotemporal characteristics of [Ca(2+)](i) transients under oscillating fluid flow are affected more profoundly by pharmacological treatments than under the steady flow. Our findings support the hypothesis that the [Ca(2+)](i) responses of osteocytic networks are significantly dependent on the profiles of fluid flow.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1016/j.bone.2012.05.021

Cites

58 of 58 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Mechanical loading stimulates osteocytes to produce anabolic factors PGE2, PGI2, NO, and IGF-1.
“For example, mechanical loading stimulates osteocyte to produce factors that exert anabolic action on bone such as PGE2, prostacyclin (PGI2), NO, and IGF-1 [181–184].”
From Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells (2015) · cites this paper as [184]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

The versatility and universality of calcium signalling
Berridge, M. J., Lipp, P., & Bootman, M. D. (2000) · Nature Reviews Molecular Cell Biology · doi:10.1038/35036035 · Cited by 5,701 · see citing works
The amazing osteocyte
Bonewald, L. F. (2011) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.320 · Cited by 2,287 · see citing works
Engineering cell shape and function
Singhvi, R., Kumar, A., Lopez, G. P., Stephanopoulos, G. N., Wang, D. I. C., Whitesides, G. M., & Ingber, D. E. (1994) · Science · doi:10.1126/science.8171320 · Cited by 1,453 · see citing works
BIOMECHANICAL AND MOLECULAR REGULATION OF BONE REMODELING
Robling, A. G., Castillo, A. B., & Turner, C. H. (2006) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.8.061505.095721 · Cited by 1,343 · see citing works
A model for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses
Weinbaum, S., Cowin, S. C., & Zeng, Y. (1994) · Journal of Biomechanics · doi:10.1016/0021-9290(94)90010-8 · Cited by 1,266 · see citing works
Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction.
Tatsumi, S., Ishii, K., Amizuka, N., Li, M., Kobayashi, T., Kohno, K., Ito, M., Takeshita, S., & Ikeda, K. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.05.001 · Cited by 848 · see citing works
Sensitivity of osteocytes to biomechanical stress in vitro
Klein‐Nulend, J., van der Plas, A., Semeins, C. M., Ajubi, N. E., Erangos, J. A., Nijweide, P. J., & Burger, E. H. (1995) · The FASEB Journal · doi:10.1096/fasebj.9.5.7896017 · Cited by 776 · see citing works
Establishment of an Osteocyte-like Cell Line, MLO-Y4
Kato, Y., Windle, J. J., Koop, B. A., Mundy, G. R., & Bonewald, L. F. (1997) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.1997.12.12.2014 · Cited by 549 · see citing works
Differential effect of steady versus oscillating flow on bone cells
Jacobs, C. R., Yellowley, C. E., DAVIS, B., Zhou, Z., Cimbala, J. M., & Donahue, H. J. (1998) · Journal of Biomechanics · doi:10.1016/s0021-9290(98)00114-6 · Cited by 453 · see citing works
Transport mechanism operating between blood supply and osteocytes in long bones
Piekarski, K. R., & Munro, M. (1977) · Nature · doi:10.1038/269080a0 · Cited by 450 · see citing works
Calcium/calmodulin signaling controls osteoblast growth and differentiation
Zayzafoon, M. (2005) · Journal of Cellular Biochemistry · doi:10.1002/jcb.20675 · Cited by 437 · see citing works
Pulsating Fluid Flow Increases Nitric Oxide (NO) Synthesis by Osteocytes but Not Periosteal Fibroblasts - Correlation with Prostaglandin Upregulation
Kleinnulend, J., Semeins, C. M., Ajubi, N. E., Nijweide, P. J., & Burger, E. H. (1995) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1995.2822 · Cited by 425 · see citing works
Five Jumps per Day Increase Bone Mass and Breaking Force in Rats
Umemura, Y., Ishiko, T., Yamauchi, T., Kurono, M., & Mashiko, S. (1997) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.1997.12.9.1480 · Cited by 421 · see citing works
Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions
Pavalko, F. M., Chen, N. X., Turner, C. H., Burr, D. B., Atkinson, S. J., Hsieh, Y., Qiu, J., & Duncan, R. L. (1998) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1998.275.6.c1591 · Cited by 404 · see citing works
Osteopontin Gene Regulation by Oscillatory Fluid Flow via Intracellular Calcium Mobilization and Activation of Mitogen-activated Protein Kinase in MC3T3–E1 Osteoblasts
You, J., Reilly, G. C., Zhen, X., Yellowley, C. E., Chen, Q., Donahue, H. J., & Jacobs, C. R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m009846200 · Cited by 390 · see citing works
Recovery periods restore mechanosensitivity to dynamically loaded bone
Robling, A. G., Burr, D. B., & Turner, C. H. (2001) · Journal of Experimental Biology · doi:10.1242/jeb.204.19.3389 · Cited by 377 · see citing works
Substrate Deformation Levels Associated With Routine Physical Activity Are Less Stimulatory to Bone Cells Relative to Loading-Induced Oscillatory Fluid Flow
You, J., Yellowley, C. E., Donahue, H. J., Zhang, Y., Chen, Q., & Jacobs, C. R. (2000) · Journal of Biomechanical Engineering · doi:10.1115/1.1287161 · Cited by 357 · see citing works
Oscillating fluid flow activation of gap junction hemichannels induces atp release from MLO‐Y4 osteocytes
Genetos, D. C., Kephart, C. J., Zhang, Y., Yellowley, C. E., & Donahue, H. J. (2007) · Journal of Cellular Physiology · doi:10.1002/jcp.21021 · Cited by 328 · see citing works
Ca2+regulates fluid shear-induced cytoskeletal reorganization and gene expression in osteoblasts
Chen, N. X., Ryder, K. D., Pavalko, F. M., Turner, C. H., Burr, D. B., Qiu, J., & Duncan, R. L. (2000) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.2000.278.5.c989 · Cited by 293 · see citing works
Functional Gap Junctions Between Osteocytic and Osteoblastic Cells
Yellowley, C. E., Li, Z., Zhou, Z., Jacobs, C. R., & Donahue, H. J. (2000) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2000.15.2.209 · Cited by 259 · see citing works
Mechanical Strain Stimulates Nitric Oxide Production by Rapid Activation of Endothelial Nitric Oxide Synthase in Osteocytes
Zaman, G. D., Pitsillides, A. A., Rawlinson, S. C. F., Suswillo, R. F. L., Mosley, J. R., Cheng, M. Z., Platts, L. A. M., Hukkanen, M., Polak, J. M., & Lanyon, L. E. (1999) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.1999.14.7.1123 · Cited by 250 · see citing works
ATP- and Gap Junction–dependent Intercellular Calcium Signaling in Osteoblastic Cells
Jørgensen, N. R., Geist, S. T., Civitelli, R., & Steinberg, T. H. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.139.2.497 · Cited by 248 · see citing works
Termination of cytosolic Ca2+ signals: Ca2+ reuptake into intracellular stores is regulated by the free Ca2+ concentration in the store lumen
Mogami, H., Tepikin, A. V., & Petersen, O. H. (1998) · The EMBO Journal · doi:10.1093/emboj/17.2.435 · Cited by 222 · see citing works
Molecular regulation of mechanotransduction
Iqbal, J. Z., & Zaidi, M. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.12.087 · Cited by 218 · see citing works
Human osteoblast-like cells produce nitric oxide and express inducible nitric oxide synthase
Ralston, S. H., Todd, D., Helfrich, M. H., Benjamin, N., & Grabowski, P. (1994) · Endocrinology · doi:10.1210/endo.135.1.7516867 · Cited by 211 · see citing works
Intracellular Ca2+ stores and extracellular Ca2+ are required in the real-time Ca2+ response of bone cells experiencing fluid flow
Hung, C. T., Allen, F. D., Pollack, S. R., & Brighton, C. T. (1996) · Journal of Biomechanics · doi:10.1016/0021-9290(96)84536-2 · Cited by 195 · see citing works
Signal transduction pathways involved in fluid flow-induced PGE2production by cultured osteocytes
Ajubi, N. E., Klein‐Nulend, J., Alblas, M. J., Burger, E. H., & Nijweide, P. J. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.276.1.e171 · Cited by 191 · see citing works
Nitric Oxide Response to Shear Stress by Human Bone Cell Cultures Is Endothelial Nitric Oxide Synthase Dependent
Klein‐Nulend, J., Helfrich, M., Sterck, J. G. H., Macpherson, H. K., Joldersma, M., Ralston, S. H., Semeins, C. M., & Burger, E. H. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9270 · Cited by 173 · see citing works
Do Bone Cells Behave Like a Neuronal Network?
Turner, C. H., Robling, A. G., Duncan, R. L., et al. (2002) · Calcified Tissue International · doi:10.1007/s00223-001-1024-z · Cited by 159 · see citing works
From streaming‐potentials to shear stress: 25 years of bone cell mechanotransduction
Riddle, R. C., & Donahue, H. J. (2009) · Journal of Orthopaedic Research · doi:10.1002/jor.20723 · Cited by 157 · see citing works
Activation of β-catenin signaling in MLO-Y4 osteocytic cells versus 2T3 osteoblastic cells by fluid flow shear stress and PGE2: Implications for the study of mechanosensation in bone
Kamel, M. A., Picconi, J. L., Lara-Castillo, N., & Johnson, M. L. (2010) · Bone · doi:10.1016/j.bone.2010.08.007 · Cited by 147 · see citing works
Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow
Lu, X. L., Huo, B., Chiang, V., & Guo, X.-D. E. (2011) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.1474 · Cited by 142 · see citing works
Self-citation
The effect of cytoskeletal disruption on pulsatile fluid flow-induced nitric oxide and prostaglandin E2 release in osteocytes and osteoblasts
McGarry, J. G., Klein‐Nulend, J., & Prendergast, P. J. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.02.175 · Cited by 139 · see citing works
Flow-induced calcium oscillations in rat osteoblasts are age, loading frequency, and shear stress dependent
Donahue, S. W., Jacobs, C. R., & Donahue, H. J. (2001) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.2001.281.5.c1635 · Cited by 124 · see citing works
A Calcium-Induced Calcium Influx Factor, Nitric Oxide, Modulates the Refilling of Calcium Stores in Astrocytes
Li, N., Sul, J., & Haydon, P. G. (2003) · Journal of Neuroscience · doi:10.1523/jneurosci.23-32-10302.2003 · Cited by 118 · see citing works
Parathyroid Hormone Enhances Fluid Shear-Induced [Ca2+]i Signaling in Osteoblastic Cells Through Activation of Mechanosensitive and Voltage-Sensitive Ca2+ Channels
Ryder, K. D., & Duncan, R. L. (2001) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2001.16.2.240 · Cited by 114 · see citing works
Human Osteoblastic Cells Propagate Intercellular Calcium Signals by Two Different Mechanisms
Jørgensen, N. R., Henriksen, Z., Brot, C., Eriksen, E. F., Sørensen, O., Civitelli, R., & Steinberg, T. H. (2000) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2000.15.6.1024 · Cited by 108 · see citing works
Osteoblastic cells have refractory periods for fluid-flow-induced intracellular calcium oscillations for short bouts of flow and display multiple low-magnitude oscillations during long-term flow
Donahue, S. W., Donahue, H. J., & Jacobs, C. R. (2002) · Journal of Biomechanics · doi:10.1016/s0021-9290(02)00318-4 · Cited by 102 · see citing works
P2Y Purinoceptors Are Responsible for Oscillatory Fluid Flow-induced Intracellular Calcium Mobilization in Osteoblastic Cells
You, J., Jacobs, C. R., Steinberg, T. H., & Donahue, H. J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m209245200 · Cited by 90 · see citing works
Inhibition of gap junction communication in alveolar epithelial cells by 18α-glycyrrhetinic acid
Guo, Y. H., Martinez-Williams, C., Gilbert, K. A., & Rannels, D. E. (1999) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.1999.276.6.l1018 · Cited by 87 · see citing works
Association of the α2δ1 subunit with Cav3.2 enhances membrane expression and regulates mechanically induced ATP release in MLO-Y4 osteocytes
Thompson, W. R., Majid, A. S., Czymmek, K. J., Ruff, A. L., Garcı́a, J., Duncan, R. L., & Farach‐Carson, M. C. (2011) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.437 · Cited by 85 · see citing works
The role of actin cytoskeleton in oscillatory fluid flow-induced signaling in MC3T3-E1 osteoblasts
Malone, A. M. D., Batra, N. N., Shivaram, G. M., et al. (2007) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00352.2005 · Cited by 85 · see citing works
An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation
Huo, B., Lu, X. L., Costa, K. D., et al. (2010) · Cell Calcium · doi:10.1016/j.ceca.2009.12.005 · Cited by 68 · see citing works
Self-citation
Fluid shear stress induction of COX-2 protein and prostaglandin release in cultured MC3T3-E1 osteoblasts does not require intact microfilaments or microtubules
Norvell, S. M., Ponik, S. M., Bowen, D. K., Gerard-O’Riley, R. L., & Pavalko, F. M. (2004) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00869.2003 · Cited by 66 · see citing works
Fluid Flow Induced Calcium Response in Bone Cell Network
Huo, B., Lu, X. L., Hung, C. T., et al. (2008) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-008-0011-0 · Cited by 66 · see citing works
Self-citation
Variable Conformation of GAP Junctions Linking Bone Cells: A Transmission Electron Microscopic Study of Linear, Stacked Linear, Curvilinear, Oval, and Annular Junctions
Shapiro, F. (1997) · Calcified Tissue International · doi:10.1007/s002239900337 · Cited by 59 · see citing works
Intracellular calcium waves in bone cell networks under single cell nanoindentation.
Guo, X.-D. E., Takai, E., Jiang, X., et al. (2006) · PubMed · Cited by 58 · see citing works
Self-citation
Nitric oxide and cGMP activate Ca2+-release processes in rat parotid acinar cells
Looms, D., Tritsaris, K., Nauntofte, B., et al. (2001) · Biochemical Journal · doi:10.1042/0264-6021:3550087 · Cited by 55 · see citing works
Primary Cultures of Chick Osteocytes Retain Functional Gap Junctions between Osteocytes and between Osteocytes and Osteoblasts
Kamioka, H., Ishihara, Y., Ris, H., Murshid, S. A., Sugawara, Y., Takano‐Yamamoto, T., & Lim, S. (2007) · Microscopy and Microanalysis · doi:10.1017/s143192760707016x · Cited by 53 · see citing works
A Trabecular Bone Explant Model of Osteocyte–Osteoblast Co-Culture for Bone Mechanobiology
Chan, M. E., Lu, X. L., Huo, B., Baik, A. D., Chiang, V., Guldberg, R. E., Lu, H. H., & Guo, X.-D. E. (2009) · Cellular and Molecular Bioengineering · doi:10.1007/s12195-009-0075-5 · Cited by 51 · see citing works
Self-citation
Mechanotransduction in Bone Cells: Induction of Nitric Oxide and Prostaglandin Synthesis by Fluid Shear Stress, but Not by Mechanical Strain
Smalt, R., Mitchell, F. T., Howard, R. L., & Chambers, T. J. (1997) · Advances in experimental medicine and biology · doi:10.1007/978-1-4899-1810-9_66 · Cited by 45 · see citing works
Extracellular ATP modulates [Ca2+]i in retinoic acid-treated embryonic chondrocytes
Hung, C. T., Allen, F. D., Mansfield, K. D., & Shapiro, I. M. (1997) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1997.272.5.c1611 · Cited by 39 · see citing works
Mechanically induced intracellular calcium waves in osteoblasts demonstrate calcium fingerprints in bone cell mechanotransduction
Godin, L. M., Suzuki, S., Jacobs, C. R., Donahue, H. J., & Donahue, S. W. (2006) · Biomechanics and Modeling in Mechanobiology · doi:10.1007/s10237-006-0059-5 · Cited by 35 · see citing works
Intercellular calcium wave propagation in linear and circuit-like bone cell networks
Huo, B., Lu, X. L., & Guo, X.-D. E. (2010) · Philosophical Transactions of the Royal Society A Mathematical Physical and Engineering Sciences · doi:10.1098/rsta.2009.0221 · Cited by 29 · see citing works
Self-citation
Chloramphenicol-induced mitochondrial dysfunction is associated with decreased transferrin receptor expression and ferritin synthesis in K562 cells and is unrelated to IRE-IRP interactions
Leiter, L. M., Thatte, H. S., Okafor, C. C., Marks, P. W., Golan, D. E., & Bridges, K. R. (1999) · Journal of Cellular Physiology · doi:10.1002/(sici)1097-4652(199909)180:3 · Cited by 29 · see citing works
Quasi-3D Cytoskeletal Dynamics of Osteocytes under Fluid Flow
Baik, A. D., Lu, X. L., Qiu, J., Huo, B., Hillman, E. M. C., Dong, C., & Guo, X.-D. E. (2010) · Biophysical Journal · doi:10.1016/j.bpj.2010.08.064 · Cited by 25 · see citing works
Self-citation
Up-regulation of Ca2+ influx mediated by store-operated channels in HL60 cells induced to differentiate by 1α,25-Dihydroxyvitamin D3
Gardner, J. P., Balasubramanyam, M., & Studzinski, G. P. (1997) · Journal of Cellular Physiology · doi:10.1002/(sici)1097-4652(199709)172:3 · Cited by 24 · see citing works
An in‐situ fluorescence‐based optical extensometry system for imaging mechanically loaded bone
Price, C., Li, W., Novotny, J. E., & Wang, L. (2009) · Journal of Orthopaedic Research® · doi:10.1002/jor.21049 · Cited by 16 · see citing works

Flagged first, then by citations

Sorted by citations

Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells
Florencio-Silva, R., Sasso, G. R. D. S., Sasso-Cerri, E., Simões, M. J., & Cerri, P. S. (2015) · BioMed Research International · doi:10.1155/2015/421746 · Cited by 2,044 · see citing works
Osteocytes: master orchestrators of bone
Schaffler, M. B., Cheung, W.-Y., Majeska, R., & Kennedy, O. (2014) · Calcified Tissue International · doi:10.1007/s00223-013-9790-y · Cited by 514 · see citing works
Mechanically induced Ca2+ oscillations in osteocytes release extracellular vesicles and enhance bone formation
Morrell, A. E., Brown, G. N., Robinson, S. T., Sattler, R. L., Baik, A. D., Zhen, G., Cao, X., Bonewald, L. F., Jin, W., Kam, L. C., et al. (2018) · Bone Research · doi:10.1038/s41413-018-0007-x · Cited by 184 · see citing works
Self-citation
UTP-induced ATP release is a fine-tuned signalling pathway in osteocytes
Kringelbach, T. M., Aslan, D., Novak, I., Schwarz, P., & Jørgensen, N. R. (2014) · Purinergic Signalling · doi:10.1007/s11302-013-9404-1 · Cited by 34 · see citing works

A106 Deformation simulation of osteocyte cell processes considering ultrastructure in bone canaliculi
YAMAMOTO, R., KAMEO, Y., OOTAO, Y., et al. (2013) · Baiofurontia Kouenkai kouen rombunshuu/Baiofurontia Koenkai koen ronbunshu · doi:10.1299/jsmebiofro.2013.24.11
Nanostructure and mechanical properties of the osteocyte lacunar-canalicular network-associated bone matrix revealed by quantitative nanomechanical mapping
Zhang, S., Bach-Gansmo, F. L., Xia, D., Besenbacher, F., Birkedal, H., & Dong, M. (2015) · Nano Research · doi:10.1007/s12274-015-0825-8 · Cited by 22 · see citing works
Osteocyte bioimaging
Kamioka, H. (2015) · Journal of Oral Biosciences · doi:10.1016/j.job.2015.02.002 · Cited by 4 · see citing works
Nanostructure and mechanical properties of the osteocyte lacunar-canalicular network-associated bone matrix revealed by quantitative nanomechanical mapping
Shuai, ., Zhang, Fiona, et al. (2015) · 纳米研究：英文版
High-resolution image-based simulation reveals membrane strain concentration on osteocyte processes caused by tethering elements
Yokoyama, Y., KAMEO, Y., Kamioka, H., & ADACHI, T. (2021) · Biomechanics and Modeling in Mechanobiology · doi:10.1007/s10237-021-01511-y · Cited by 21 · see citing works

Authors: Lu, X. L., Huo, B., Park, M., & Guo, X. E.
Affiliations: Columbia University (US)
Chinese Academy of Sciences (CN)
ORCID: Lu, X. Lucas
Huo, Bo
Venue: Bone
Publisher: Elsevier BV
Published: 2012-09
Volume: 51
Issue: 3
Pages: 466-473
ISSN: 1873-2763 (ISSN-L)
8756-3282
DOI: 10.1016/j.bone.2012.05.021
PMC: PMC3412915
OpenAlex: W1983878512
Cited by: 105
References: 58
Citations by year: 2026: 3 · 2025: 6 · 2024: 9 · 2023: 7 · 2022: 2 · 2021: 9 · 2020: 9 · 2019: 11 · 2018: 4 · 2017: 9 · 2016: 10 · 2015: 8 · 2014: 8 · 2013: 10
FWCI: 3.35
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Bone (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
中国科学院力学研究所 (submittedVersion)
Funders: National Institutes of Health (AR052417, AR058453)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (R21AR059917, R01AR052461, RC1AR058453, R21AR052417)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone health and osteoporosis research
Keywords: osteocytes, intracellular calcium signaling, fluid shear stress, oscillatory flow, steady fluid flow, lacunar-canalicular system, MLO-Y4 cells, micropatterning, endoplasmic reticulum calcium stores, extracellular calcium, ATP, prostaglandin E2, nitric oxide, mechanotransduction
MeSH terms: Animals, Calcium, Cell Count, Cell Line, Humans, Models, Biological, Oscillometry, Osteocytes, Rheology, Time Factors, Calcium Signaling, Mice, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Calcium response in osteocytic networks under steady and oscillatory fluid flow

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Self-check

Cites

Cited by

Related

Record

About these checks