Autoantibodies against neuronal progenitors in sera from children with autism
Abstract
The pathological role of autoantibodies in development of CNS disorders is a new idea with growing interest among neuroscientists. The involvement of autoimmune response in the pathogenesis of autism spectrum disorders (ASD) has been suggested by the presence of multiple brain-specific autoantibodies in children with ASD and in their mothers. The possibility of the effect of autoimmunity on neurogenesis and postnatal brain plasticity has not been determined. The presence of autoantibodies against human neuronal progenitor cells (NPCs) stimulated for neuronal differentiation in culture was tested in sera from children with autism (n=20) and age-matched controls (n=18) by immunoblotting and immunocytochemistry. Immunoreactivity against multiple NPCs proteins of molecular sizes of approximately 55 kDa, 105 kDa, 150 kDa, and 210 kDa in sera from individuals with autism had a higher incidence and was stronger than in control sera which immunoreacted mainly with a 150 kDa protein. The sera from children with autism immunoreacted the strongest with NPCs expressing neuronal markers Tuj1 and doublecortin, but not astrocyte marker GFAP. The epitopes recognized by antibodies from sera were not human-specific because they detected also NPCs in situ in murine hippocampus. The autoimmune reactions against NPCs suggest an impaired tolerance to neural antigens in autism. These autoantibodies may be symptomatic for autism and furthermore, their presence suggests that autoimmunity may affect postnatal neuronal plasticity particularly after impairment of blood-brain barrier. Future studies will determine the diagnostic value of the presence of autoantibodies in autism and the therapeutic value of prevention of autoimmunity in autism.
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Among positive samples, those with ASD immunostained more strongly than controls.
“and among positive samples, those with ASD immunostained more strongly than controls (Mazur-Kolecka et al, 2014).”
From The Role of the Immune System in Autism Spectrum Disorder (2017) · cites this paper as Mazur-Kolecka et al, 2014
Autoimmunoreactivity to neuronal progenitor cells was far more common in children with autism than in controls.
“A later study found autoimmunoreactivity to neuronal progenitor cells (NPCs) to be far more common in children with autism than controls”
From The Role of the Immune System in Autism Spectrum Disorder (2017) · cites this paper as Mazur-Kolecka et al, 2014
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The Role of the Immune System in Autism Spectrum Disorder
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- Authors
- Mazur-Kolecka, B., Cohen, I. L., Gonzalez, M., Jenkins, E. C., Kaczmarski, W., Brown, W. T., Flory, M., & Frackowiak, J.
- Venue
- Brain and Development
- Publisher
- Elsevier BV
- Published
- 2014-04
- Volume
- 36
- Issue
- 4
- Pages
- 322-329
- ISSN
- 0387-7604 (ISSN-L)
- OpenAlex
- W2149908382
- Cited by
- 29
- References
- 40
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 7 · 2017: 2 · 2016: 1 · 2015: 5 · 2014: 7
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain and Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Office for People With Developmental Disabilities
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Autism Spectrum Disorder Research
- Health Sciences › Medicine › Neurology › Autoimmune Neurological Disorders and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Keywords
- autoantibodies, autism spectrum disorder, neural progenitor cells, autoimmunity, neurogenesis, Tuj1, doublecortin, blood-brain barrier
- MeSH terms
- Doublecortin Domain Proteins, Animals, Autistic Disorder, Autoantibodies, Cells, Cultured, Child, Preschool, Female, Fluorescent Antibody Technique, Glial Fibrillary Acidic Protein, Hippocampus, Humans, Immunohistochemistry, Infant, Male, Microtubule-Associated Proteins, Nerve Tissue Proteins, Neuropeptides, Tubulin, Immunoblotting, Mice, Neurogenesis, Neural Stem Cells
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