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Cited by 60Sleep and Wakefulness ResearchSleep and Work-Related FatigueCircadian rhythm and melatonin

Cortical metabolic rates as measured by 2-deoxyglucose-uptake are increased after waking and decreased after sleep in mice

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Vyazovskiy, V.V., Cirelli, C., Tononi, G., Tobler, I.·2008·Brain Research Bulletin
DOI 10.1016/j.brainresbull.2007.10.040PMC PMC2386085
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Abstract

A recent hypothesis suggests that a major function of sleep is to renormalize synaptic changes that occur during wakefulness as a result of learning processes [G. Tononi, C. Cirelli, Sleep and synaptic homeostasis: a hypothesis, Brain Res. Bull. 62 (2003) 143-150; G. Tononi, C. Cirelli, Sleep function and synaptic homeostasis, Sleep Med. Rev. 10 (2006) 49-62]. Specifically, according to this synaptic homeostasis hypothesis, wakefulness results in a net increase in synaptic strength, while sleep is associated with synaptic downscaling. Since synaptic activity accounts for a large fraction of brain energy metabolism, one of the predictions of the hypothesis is that if synaptic weight increases in the course of wakefulness, cerebral metabolic rates should also increase, while the opposite would happen after a period of sleep. In this study we therefore measured brain metabolic rate during wakefulness and determined whether it was affected by the previous sleep-wake history. Three groups of mice in which behavioral states were determined by visual observation were subjected to 6h of sleep deprivation (SD). Group 1 was injected with 2-deoxyglucose (2-DG) 45 min before the end of SD, while Group 2 and Group 3 were injected with 2-DG after an additional period (2-3h) of waking or sleep, respectively. During the 45-min interval between 2-DG injection and sacrifice all mice were kept awake. We found that in mice that slept approximately 2.5h the 2-DG-uptake was globally decreased, on average by 15-20%, compared to the first two groups that were kept awake. On average, Group 2, which stayed awake approximately 2h more than Group 1, showed only a small further increase in 2-DG-uptake relative to Group 1. Moreover, the brain regions in which 2-DG-uptake increased the least when waking was prolonged by approximately 2h showed the most pronounced decrease in DG-uptake after sleep. The data are consistent with the prediction that sleep may reset cerebral metabolic rates to a lower level.

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  • In mice, cerebral metabolism measured by 2-deoxyglucose uptake increased after periods of wakefulness and decreased after sleep.

    “Indeed, cerebral metabolism measured by 2-deoxyglucose uptake increased after periods of wakefulness and decreased after sleep in mice (1265).”

    From About Sleep's Role in Memory (2013) · cites this paper as [1265]

  • Hemispheric differences in SWA during sleep in rats were related to the preferential use of the left or right paw during the day.

    “Thus hemispheric differences in SWA during sleep in rats were related to the preferential use of the left or right paw during the day (1265).”

    From About Sleep's Role in Memory (2013) · cites this paper as [1265]

    The Materials and Methods (sections 2.1 and 2.3; page unavailable) describe mice, unilateral whisker stimulation, and measurements of 2-DG uptake, not paw preference in rats. The Discussion (page unavailable) says EEG was not recorded in this study. Searches for paw preference and hemispheric SWA found no passage reporting the claimed relationship. (LLM, conf=0.75)

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Research works printed but not linked: 1

  1. [24]Pfister-Genskow M, Cirelli C, Tononi G. Molecular evidence for synaptic potentiation during waking and synaptic downscaling during sleep. Sleep. 2007;30:A5–A6. [Google Scholar]

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Authors
Vyazovskiy, V. V., Cirelli, C., Tononi, G., & Tobler, I.
Affiliations
  • University of Zurich (CH)
  • University of Wisconsin–Madison (US)
ORCID
Venue
Brain Research Bulletin
Publisher
Elsevier BV
Published
2008-03
Volume
75
Issue
5
Pages
591-597
ISSN
0361-9230 (ISSN-L)
DOI
10.1016/j.brainresbull.2007.10.040
PMC
PMC2386085
OpenAlex
W2061234946
Cited by
60
References
36
Citations by year
2026: 3 · 2025: 3 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2019: 2 · 2018: 2 · 2017: 6 · 2016: 2 · 2015: 4 · 2014: 6 · 2013: 4 · 2012: 5
FWCI
2.50
Type
journal article
Language
en
Open access
Licence
Locations
  • Brain Research Bulletin (publishedVersion)
  • PubMed (publishedVersion)
  • Zurich Open Repository and Archive (University of Zurich) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • Open Research (University of Surrey) (submittedVersion)
  • Surrey Research Insight Open Access (The University of Surrey) (submittedVersion)
  • Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
  • Zurich Open Repository and Archive (University of Zurich)
Funders
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (320000, 100567)
  • National Institutes of Health (5p20mh077967-04, DP1OD000579)
  • National Institute of Mental Health (P20MH-077967)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
  • Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
Keywords
sleep, wakefulness, cerebral metabolic rate, 2-deoxyglucose uptake, synaptic homeostasis hypothesis, sleep-wake cycle, sleep deprivation, brain energy metabolism, synaptic scaling, mice
MeSH terms
Animals, Brain Mapping, Cerebral Cortex, Deoxyglucose, Male, Radiography, Sleep, Wakefulness, Mice

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