The DOI matches its Crossref record; 25 of the 26 references it cites were found in a registry or an index.
MicroRNA-21 down-regulates the expression of tumor suppressor PDCD4 in human glioblastoma cell T98G
Abstract
MicroRNAs have been linked to different cancer-related processes. The microRNA miR-21 appears to function as an anti-apoptosis factor in glioblastomas. However, the functional target genes of miR-21 are largely unknown in glioblastomas. In this study, bioinformatics analysis was used to identify miR-21 target sites in various genes. Luciferase activity assay showed that a number of genes involved in apoptosis, PDCD4, MTAP, and SOX5, carry putative miR-21 binding sites. Expression of PDCD4 protein correlates inversely with expression of miR-21 in a number of human glioblastoma cell lines such as T98G, A172, U87, and U251. Inhibition of miR-21 increases endogenous levels of PDCD4 in cell line T98G and over-expression miR-21 inhibits PDCD4-dependent apoptosis. Together, these results indicate that miR-21 expression plays a key role in regulating cellular processes in glioblastomas and may serve as a target for effective therapies.
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“In particular, miRNA-21 (miR-21) has been shown to be a strong antiapoptotic factor [5, 9–11].”
From MicroRNA‐21 protects neurons from ischemic death (2010) · cites this paper as [9]
In the citing paper
“Previous studies have proven that PDCD4, HNRNPK and RECK are TSGs among the direct targets of miR-21-5p (13,(27)(28)(29).”
From Functional interaction between miR-505-3p and miR-21 contributes to GBM suppression (2026) · cites this paper as [27]
Citations
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1 of the 26 references couldn’t be matched to a known paper.
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MicroRNAs in Development and Disease
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Grimm, D. G., Bauer, J. W., Wise, P. M., Krüger, M., Simonsen, U., Wehland, M., Infanger, M., & Corydon, T. J. (2019) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2019.03.004 · Cited by 493
Apoptosis and the target genes of microRNA-21
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Metadata
- Authors
- Chen, Y., Liu, W., Chao, T., Zhang, Y., Yan, X., Gong, Y., Qiang, B., Yuan, J., Sun, M., & Peng, X.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Corresponding author
- Sun, Maosheng
- Peng, Xiaozhong
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 2008-12
- Volume
- 272
- Issue
- 2
- Pages
- 197-205
- ISSN
- 0304-3835 (ISSN-L)
- OpenAlex
- W2091108445
- Cited by
- 217
- References
- 26
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 7 · 2022: 2 · 2021: 7 · 2020: 4 · 2019: 10 · 2018: 7 · 2017: 13 · 2016: 10 · 2015: 17 · 2014: 24 · 2013: 19 · 2012: 20
- FWCI
- 8.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cancer Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Health Sciences › Medicine › Oncology › Glioma Diagnosis and Treatment
- Keywords
- miR-21, PDCD4, glioblastoma, microRNA binding sites, apoptosis, MTAP, SOX5, T98G cells, tumor suppressor regulation, miRNA inhibitors
- MeSH terms
- Base Sequence, Brain Neoplasms, Glioblastoma, Humans, Blotting, Northern, Blotting, Western, Down-Regulation, Gene Expression Regulation, Neoplastic, RNA-Binding Proteins, DNA Primers, Reverse Transcriptase Polymerase Chain Reaction, In Situ Nick-End Labeling, MicroRNAs, Cell Line, Tumor, Apoptosis Regulatory Proteins
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