miR-21-mediated tumor growth
Abstract
MicroRNAs (miRNAs) are approximately 22 nucleotide non-coding RNA molecules that regulate gene expression post-transcriptionally. Although aberrant expression of miRNAs in various human cancers suggests a role for miRNAs in tumorigenesis, it remains largely unclear as to whether knockdown of a specific miRNA affects tumor growth. In this study, we profiled miRNA expression in matched normal breast tissue and breast tumor tissues by TaqMan real-time polymerase chain reaction miRNA array methods. Consistent with previous findings, we found that miR-21 was highly overexpressed in breast tumors compared to the matched normal breast tissues among 157 human miRNAs analysed. To better evaluate the role of miR-21 in tumorigenesis, we transfected breast cancer MCF-7 cells with anti-miR-21 oligonucleotides and found that anti-miR-21 suppressed both cell growth in vitro and tumor growth in the xenograft mouse model. Furthermore, this anti-miR-21-mediated cell growth inhibition was associated with increased apoptosis and decreased cell proliferation, which could be in part owing to downregulation of the antiapoptotic Bcl-2 in anti-miR-21-treated tumor cells. Together, these results suggest that miR-21 functions as an oncogene and modulates tumorigenesis through regulation of genes such as bcl-2 and thus, it may serve as a novel therapeutic target.
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Modulating miR-21 expression in in vivo and in vitro studies shows clear and important phenotype changes.
“Modulating its expression in in vivo and in vitro studies show clear and important phenotype changes.”
From miR-21: a non‐specific biomarker of all maladies (2021) · cites this paper as [19]
miR-21 has been implicated in cellular proliferation, apoptosis, and migration in carcinogenesis.
“This microRNA has been implicated in various aspects of carcinogenesis, including cellular proliferation, apoptosis, and migration.”
From MicroRNA 21 Promotes Glioma Invasion by Targeting Matrix Metalloproteinase Regulators (2008) · cites this paper as [51]
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- Authors
- Si, M.-L., Zhu, S., Wu, H., Lu, Z., Wu, F., & Mo, Y.-Y.
- Affiliations
- SIU Medicine (US)
- Corresponding author
- Mo, Y-Y
- Venue
- Oncogene
- Published
- 2006-10-30
- Volume
- 26
- Issue
- 19
- Pages
- 2799-2803
- ISSN
- 0950-9232 (ISSN-L)
- 1476-5594
- OpenAlex
- W2103211592
- Cited by
- 1,600
- References
- 3
- Citations by year
- 2026: 20 · 2025: 31 · 2024: 38 · 2023: 46 · 2022: 56 · 2021: 59 · 2020: 67 · 2019: 68 · 2018: 67 · 2017: 82 · 2016: 94 · 2015: 116 · 2014: 111 · 2013: 111 · 2012: 117
- FWCI
- 33.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Oncogene (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (R01CA102630, CA 102630)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- miR-21, microRNA, breast cancer, tumor growth, anti-miR-21, Bcl-2, apoptosis, cell proliferation, miRNA expression profiling, xenograft tumor model, therapeutic target
- MeSH terms
- Animals, Breast, Breast Neoplasms, Female, Humans, Mice, Nude, RNA Processing, Post-Transcriptional, RNA, Messenger, Gene Expression Regulation, Neoplastic, Oligonucleotides, Antisense, Apoptosis, Proto-Oncogene Proteins c-bcl-2, Xenograft Model Antitumor Assays, MicroRNAs, Cell Proliferation, Mice
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