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Efficient Intracellular Delivery of Native Proteins
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iTOP was used to deliver CRISPR/Cas9 RNP into primary human KBM7 and H1 cells, conferring diphtheria toxin resistance with approximately 70% gene knockout efficiency.
“iTOP was used to delivery CRISPR/Cas9 RNP into primary human KBM7 and H1 cells, conferring diphtheria toxin resistance with ∼70% gene knockout efficiency.”
From Delivering CRISPR: a review of the challenges and approaches (2018) · cites this paper as D'Astolfo et al., 2015
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Cites
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Delivering CRISPR: a review of the challenges and approaches
Lino, C. A., Harper, J. C., Carney, J. P., & Timlin, J. A. (2018) · Drug Delivery · doi:10.1080/10717544.2018.1474964 · Cited by 1,185
Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles
Wang, M., Zuris, J. A., Meng, F., Rees, H., Sun, S., Deng, P., Han, Y., Gao, X., Pouli, D., Wu, Q., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1520244113 · Cited by 624
Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications
Liu, C., Zhang, L., Liu, H., & Cheng, K. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.09.012 · Cited by 607
Delivery technologies for genome editing
Yin, H., Kauffman, K. J., & Anderson, D. G. (2017) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.280 · Cited by 597
Wang, H.-X., Li, M., Lee, C. M., Chakraborty, S., Kim, H.-W., Bao, G., & Leong, K. W. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00799 · Cited by 560
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Cheng, G., Li, W., Ha, L., Han, X., Hao, S., Wan, Y., Wang, Z., Dong, F., Zou, X., Mao, Y., et al. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.8b03584 · Cited by 371
3D model for CAR‐mediated cytotoxicity using patient‐derived colorectal cancer organoids
Schnalzger, T. E., de Groot, M. H., Zhang, C., Mosa, M. H., Michels, B. E., Röder, J., Darvishi, T., Wels, W. S., & Farin, H. F. (2019) · The EMBO Journal · doi:10.15252/embj.2018100928 · Cited by 354
Engineering the delivery system for CRISPR-based genome editing
Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.11.006 · Cited by 345
Uses and abuses of macropinocytosis
Bloomfield, G., & Kay, R. R. (2016) · Journal of Cell Science · doi:10.1242/jcs.176149 · Cited by 193
Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells
Ting, P. Y., Parker, A. E., Lee, J. S., Trussell, C., Sharif, O., Luna, F., Federe, G., Barnes, S. W., Walker, J. R., Vance, J., et al. (2018) · Nature Methods · doi:10.1038/s41592-018-0149-1 · Cited by 80
Chakraborty, S., Wenzlitschke, N., Anderson, M. J., Eraso, A., Baudic, M., Thompson, J. J., Evans, A. A., Shatford-Adams, L. M., Chari, R., Awasthi, P., et al. (2025) · Developmental Cell · doi:10.1016/j.devcel.2025.02.002 · Cited by 22
Engineered retrons generate genome-independent protein-binding DNA for cellular control
Lee, G., & Kim, J. (2023) · doi:10.1101/2023.09.27.556556 · Cited by 15
Metadata
- Authors
- D’Astolfo, D. S., Pagliero, R. J., Pras, A., Karthaus, W. R., Clevers, H., Prasad, V., Lebbink, R. J., Rehmann, H., & Geijsen, N.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2015-04
- Volume
- 161
- Issue
- 3
- Pages
- 674-690
- ISSN
- 0092-8674
- Cited by
- 338
- References
- 3
- Language
- en
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