Engineering the delivery system for CRISPR-based genome editing

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116 · see citing works

Multiplex Genome Engineering Using CRISPR/Cas Systems

Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339 · see citing works

RNA-Guided Human Genome Engineering via Cas9

Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210 · see citing works

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437 · see citing works

Liposomal drug delivery systems: From concept to clinical applications

Allen, T. M., & Cullis, P. R. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.09.037 · Cited by 4,839 · see citing works

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., John van der, O. O. S. T., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,750 · see citing works

In vivo genome editing using Staphylococcus aureus Cas9

Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., et al. (2015) · Nature · doi:10.1038/nature14299 · Cited by 2,687 · see citing works

CRISPR/Cas, the Immune System of Bacteria and Archaea

Horvath, P., & Barrangou, R. (2010) · Science · doi:10.1126/science.1179555 · Cited by 2,471 · see citing works

A TALE nuclease architecture for efficient genome editing

Miller, J. C., Tan, S., Qiao, G., Barlow, K. A., Wang, J., Xia, D. F., Meng, X., Paschon, D. E., Leung, E., Hinkley, S. J., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1755 · Cited by 2,098 · see citing works

Nanoparticle PEGylation for Imaging and Therapy

Jokerst, J. V., Lobovkina, T., Zare, R. N., & Gambhir, S. S. (2011) · Nanomedicine · doi:10.2217/nnm.11.19 · Cited by 2,054 · see citing works

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

Kim, S., Kim, D., Cho, S. W., Kim, J., & Kim, J.-S. (2014) · Genome Research · doi:10.1101/gr.171322.113 · Cited by 1,856 · see citing works

Highly efficient endogenous human gene correction using designed zinc-finger nucleases

Urnov, F. D., Miller, J. C., Lee, Y.-L., Beausejour, C. M., Rock, J. M., Augustus, S., Jamieson, A. C., Porteus, M. H., Gregory, P. D., & Holmes, M. C. (2005) · Nature · doi:10.1038/nature03556 · Cited by 1,642 · see citing works

Challenges and Key Considerations of the Enhanced Permeability and Retention Effect for Nanomedicine Drug Delivery in Oncology

Prabhakar, U., Maeda, H., Jain, R. K., Sevick-Muraca, E. M., Zamboni, W. C., Farokhzad, O. C., Barry, S. T., Gabizón, A., Grodzinski, P. A., & Blakey, D. C. (2013) · Cancer Research · doi:10.1158/0008-5472.can-12-4561 · Cited by 1,493 · see citing works

Analysis of AAV Serotypes 1–9 Mediated Gene Expression and Tropism in Mice After Systemic Injection

Zincarelli, C., Soltys, S., Rengo, G., & Rabinowitz, J. E. (2008) · Molecular Therapy · doi:10.1038/mt.2008.76 · Cited by 1,490 · see citing works

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

Zuris, J. A., Thompson, D. B., Shu, Y., Guilinger, J. P., Bessen, J. L., Hu, J. H., Maeder, M. L., Joung, J. K., Chen, Z.-Y., & Liu, D. R. (2015) · Nature Biotechnology · doi:10.1038/nbt.3081 · Cited by 1,460 · see citing works

Adeno-Associated Virus (AAV) as a Vector for Gene Therapy

Naso, M. F., Tomkowicz, B., Perry, W. L., & Strohl, W. R. (2017) · BioDrugs · doi:10.1007/s40259-017-0234-5 · Cited by 1,362 · see citing works

Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

Jinek, M., Jiang, F., Taylor, D. W., Sternberg, S. H., Kaya, E., Ma, E., Anders, C., Hauer, M., Zhou, K., Lin, S., et al. (2014) · Science · doi:10.1126/science.1247997 · Cited by 1,230 · see citing works

In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy

Nelson, C. E., Hakim, C. H., Ousterout, D. G., Thakore, P. I., Moreb, E. A., Rivera, R. M. C., Madhavan, S., Pan, X., Ran, F. A., Yan, W. X., et al. (2016) · Science · doi:10.1126/science.aad5143 · Cited by 1,144 · see citing works

In vivo gene editing in dystrophic mouse muscle and muscle stem cells

Tabebordbar, M., Zhu, K., Cheng, J. K. W., Chew, W. L., Widrick, J. J., Yan, W. X., Maesner, C., Wu, E. Y., Xiao, R., Ran, F. A., et al. (2016) · Science · doi:10.1126/science.aad5177 · Cited by 1,046 · see citing works

Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes

Pardi, N., Tuyishime, S., Muramatsu, H., Kariko, K., Mui, B. L., Tam, Y. K., Madden, T. D., Hope, M. J., & Weissman, D. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.08.007 · Cited by 1,014 · see citing works

Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype

Yin, H., Xue, W., Chen, S., Bogorad, R. L., Benedetti, E., Grompe, M., Koteliansky, V., Sharp, P. A., Jacks, T., & Anderson, D. G. (2014) · Nature Biotechnology · doi:10.1038/nbt.2884 · Cited by 951 · see citing works

Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular dystrophy

Long, C., Amoasii, L., Mireault, A. A., McAnally, J., Li, H. Z., Sanchez‐Ortiz, E., Bhattacharyya, S., Shelton, J. M., Bassel‐Duby, R., & Olson, E. N. (2015) · Science · doi:10.1126/science.aad5725 · Cited by 941 · see citing works

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

Friedland, A. E., Tzur, Y. B., Esvelt, K. M., Colaiácovo, M. P., Church, G. M., & Calarco, J. A. (2013) · Nature Methods · doi:10.1038/nmeth.2532 · Cited by 899 · see citing works

Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo

Yin, H., Song, C.-Q., Dorkin, J. R., Zhu, L. J., Li, Y., Wu, Q., Park, A., Yang, J., Suresh, S., Bizhanova, A., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3471 · Cited by 876 · see citing works

Cancer active targeting by nanoparticles: a comprehensive review of literature

Bazak, R., Houri, M., Achy, S. E., Kamel, S., & Refaat, T. (2014) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-014-1767-3 · Cited by 766 · see citing works

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair

Lee, K., Conboy, M., Park, H. M., Jiang, F., Kim, H. J., Dewitt, M. A., Mackley, V. A., Chang, K., Rao, A., Skinner, C., et al. (2017) · Nature Biomedical Engineering · doi:10.1038/s41551-017-0137-2 · Cited by 755 · see citing works

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

Liang, X., Potter, J., Kumar, S., Zou, Y., Quintanilla, R., Sridharan, M., Carte, J., Chen, W., Roark, N., Ranganathan, S., et al. (2015) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2015.04.024 · Cited by 733 · see citing works

Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

Kim, D., Kim, J., Hur, J. K., Been, K. W., Yoon, S.-H., & Kim, J.-S. (2016) · Nature Biotechnology · doi:10.1038/nbt.3609 · Cited by 731 · see citing works

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

Ramakrishna, S., Kwaku Dad, A.-B., Beloor, J., Gopalappa, R., Lee, S.-K., & Kim, H. (2014) · Genome Research · doi:10.1101/gr.171264.113 · Cited by 655 · see citing works

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

Li, D., Qiu, Z., Shao, Y., Chen, Y., Guan, Y., Liu, M., Li, Y., Wang, L., Wang, L., Lu, X., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2661 · Cited by 639 · see citing works

Self‐assembled DNA nanoclews for the efficient delivery of CRISPR–Cas9 for genome editing

Sun, W., Ji, W., Hall, J. M., Hu, Q., Wang, C., Beisel, C. L., & Gu, Z. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201506030 · Cited by 629 · see citing works

Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles

Wang, M., Zuris, J. A., Meng, F., Rees, H., Sun, S., Deng, P., Han, Y., Gao, X., Pouli, D., Wu, Q., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1520244113 · Cited by 624 · see citing works

Direct cytosolic delivery of CRISPR/Cas9-ribonucleoprotein for efficient gene editing

Mout, R., Ray, M., Yesilbag Tonga, G., Lee, Y.-W., Tay, T., Sasaki, K., & Rotello, V. M. (2017) · ACS Nano · doi:10.1021/acsnano.6b07600 · Cited by 579 · see citing works

CRISPR/Cas9-based genome editing for disease modeling and therapy: challenges and opportunities for nonviral delivery

Wang, H.-X., Li, M., Lee, C. M., Chakraborty, S., Kim, H.-W., Bao, G., & Leong, K. W. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00799 · Cited by 560 · see citing works

Effect of liposome size on the circulation time and intraorgan distribution of amphipathic poly(ethylene glycol)-containing liposomes

Litzinger, D. C., Buiting, A. M. J., van Rooijen, N., & Huang, L. (1994) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(94)90038-8 · Cited by 546 · see citing works

Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases

Tsai, S. Q., & Joung, J. K. (2016) · Nature Reviews Genetics · doi:10.1038/nrg.2016.28 · Cited by 508 · see citing works

Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

Yin, H., Song, C.-Q., Suresh, S., Wu, Q., Walsh, S., Rhym, L. H., Mintzer, E., Bolukbasi, M. F., Zhu, L. J., Kauffman, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.4005 · Cited by 499 · see citing works

Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy

Ousterout, D. G., Kabadi, A. M., Thakore, P. I., Majoros, W. H., Reddy, T. E., & Gersbach, C. A. (2015) · Nature Communications · doi:10.1038/ncomms7244 · Cited by 470 · see citing works

Expanding the Biologist’s Toolkit with CRISPR-Cas9

Sternberg, S. H., & Doudna, J. A. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.02.032 · Cited by 398 · see citing works

Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes

Staahl, B. T., Benekareddy, M., Coulon-Bainier, C., Banfal, A. A., Floor, S. N., Sabo, J. K., Urnes, C., Munares, G. A., Ghosh, A., & Doudna, J. A. (2017) · Nature Biotechnology · doi:10.1038/nbt.3806 · Cited by 355 · see citing works

Efficient Intracellular Delivery of Native Proteins

D’Astolfo, D. S., Pagliero, R. J., Pras, A., Karthaus, W. R., Clevers, H. C., Prasad, V., Lebbink, R. J., Rehmann, H., & Geijsen, N. (2015) · Cell · doi:10.1016/j.cell.2015.03.028 · Cited by 343 · see citing works

Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells

Liao, H., Gu, Y., Diaz, A., Marlett, J. M., Takahashi, Y., Li, M., Suzuki, K., Xu, R., Hishida, T., Chang, C.-J., et al. (2015) · Nature Communications · doi:10.1038/ncomms7413 · Cited by 338 · see citing works

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

Hashimoto, M., & Takemoto, T. (2015) · Scientific Reports · doi:10.1038/srep11315 · Cited by 326 · see citing works

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes

Crispo, M., Mulet, A. P., Tesson, L., Barrera, N., Cuadro, F., dos Santos-Neto, P. C., Nguyen, T. H., Crénéguy, A., Brusselle, L., Anegón, I., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0136690 · Cited by 310 · see citing works

A non-viral CRISPR/Cas9 delivery system for therapeutically targeting HBV DNA and pcsk9 in vivo

Jiang, C., Mei, M., Li, B., Zhu, X., Zu, W., Tian, Y., Wang, Q., Guo, Y., Dong, Y., & Tan, X. (2017) · Cell Research · doi:10.1038/cr.2017.16 · Cited by 295 · see citing works

Targetability of novel immunoliposomes modified with amphipathic poly(ethylene glycol) s conjugated at their distal terminals to monoclonal antibodies

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Advances in therapeutic CRISPR/Cas9 genome editing

Savić, N., & Schwank, G. (2016) · Translational Research · doi:10.1016/j.trsl.2015.09.008 · Cited by 257 · see citing works

Reactive Oxygen Species‐Responsive Protein Modification and Its Intracellular Delivery for Targeted Cancer Therapy

Wang, M., Sun, S., Neufeld, C. I., Pérez-Ramírez, B., & Xu, Q. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201407234 · Cited by 249 · see citing works

CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein

Tang, L., Zeng, Y., Du, H., Gong, M., Peng, J., Zhang, B., Lei, M., Zhao, F., Wang, W., Li, X., et al. (2017) · Molecular Genetics and Genomics · doi:10.1007/s00438-017-1299-z · Cited by 220 · see citing works

Systemic delivery of modified mRNA encoding herpes simplex virus 1 thymidine kinase for targeted cancer gene therapy.

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Cell-penetrating peptides: Possible transduction mechanisms and therapeutic applications

Guo, Z.-R., Peng, H., Kang, J., & Sun, D. (2016) · Biomedical Reports · doi:10.3892/br.2016.639 · Cited by 171 · see citing works

Engineering CRISPR–Cpf1 crRNAs and mRNAs to maximize genome editing efficiency

Li, B., Zhao, W., Luo, X. L., Zhang, X., Li, C., Zeng, C., & Dong, Y. (2017) · Nature Biomedical Engineering · doi:10.1038/s41551-017-0066 · Cited by 137 · see citing works

Hydrodynamic Gene Delivery and Its Applications in Pharmaceutical Research

Bonamassa, B., Hai, L., & Liu, D. (2010) · Pharmaceutical Research · doi:10.1007/s11095-010-0338-9 · Cited by 118 · see citing works

The role of dioleoylphosphatidylethanolamine (DOPE) in targeted gene delivery with mannosylated cationic liposomes via intravenous route

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Non-viral delivery of genome-editing nucleases for gene therapy

Wang, M., Glass, Z., & Xu, Q. (2016) · Gene Therapy · doi:10.1038/gt.2016.72 · Cited by 110 · see citing works

Enhanced Intracellular siRNA Delivery using Bioreducible Lipid‐Like Nanoparticles

Wang, M., Alberti, K. A., Varone, A., Pouli, D., Georgakoudi, I., & Xu, Q. (2014) · Advanced Healthcare Materials · doi:10.1002/adhm.201400039 · Cited by 101 · see citing works

Delivery of Viral Vectors to Tumor Cells: Extracellular Transport, Systemic Distribution, and Strategies for Improvement

Wang, Y., & Yuan, F. (2006) · Annals of Biomedical Engineering · doi:10.1007/s10439-005-9007-2 · Cited by 72 · see citing works

Effective delivery with enhanced translational activity synergistically accelerates mRNA-based transfection

Zohra, F. T., Chowdhury, E. H., Tada, S., Hoshiba, T., & Akaike, T. (2007) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2007.04.059 · Cited by 71 · see citing works

Nanoparticles for CRISPR–Cas9 delivery

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Combinatorial Library Strategies for Synthesis of Cationic Lipid-Like Nanoparticles and their Potential Medical Applications

Altınoglu, S., Wang, M., & Xu, Q. (2015) · Nanomedicine · doi:10.2217/nnm.14.192 · Cited by 68 · see citing works

Nanopolymeric Therapeutics

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Intracellular delivery of the PTEN protein using cationic lipidoids for cancer therapy

Altınoglu, S., Wang, M., Li, K. Q., Li, Y., & Xu, Q. (2016) · Biomaterials Science · doi:10.1039/c6bm00580b · Cited by 39 · see citing works

A Simple, Highly Efficient Method for Heterologous Expression in Mammalian Primary Neurons Using Cationic Lipid-mediated mRNA Transfection

Williams, D. J., Puhl, H. L., & Ikeda, S. R. (2010) · Frontiers in Neuroscience · doi:10.3389/fnins.2010.00181 · Cited by 38 · see citing works

Immunogenicity of intrathecal plasmid gene delivery: cytokine release and effects on transgene expression

Hughes, T. S., Langer, S. J., Virtanen, S. I., Chavez, R. A., Watkins, L. R., Milligan, E. D., & Leinwand, L. A. (2009) · The Journal of Gene Medicine · doi:10.1002/jgm.1364 · Cited by 28 · see citing works

Image-Guided Hydrodynamic Gene Delivery: Current Status and Future Directions

Kamimura, K., Yokoo, T., Abé, H., Kobayashi, Y., Ogawa, K., Shinagawa, Y., Inoue, R., & Terai, S. (2015) · Pharmaceutics · doi:10.3390/pharmaceutics7030213 · Cited by 26 · see citing works

Tailoring non-viral delivery vehicles for transporting genome-editing tools

Sun, W., & Gu, Z. (2016) · Science China Materials · doi:10.1007/s40843-016-5154-4 · Cited by 16 · see citing works

Membrane Microdomain Structures of Liposomes and Their Contribution to the Cellular Uptake Efficiency into HeLa Cells

Onuki, Y., Obata, Y., Kawano, K., Sano, H., Matsumoto, R., Hayashi, Y., & Takayama, K. (2015) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.5b00601 · Cited by 14 · see citing works

CRISPR-Cas guides the future of genetic engineering

Knott, G. J., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aat5011 · Cited by 1,410 · see citing works

Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery

Kowalski, P. S., Rudra, A., Miao, L., & Anderson, D. G. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.02.012 · Cited by 1,127 · see citing works

Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts

Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 748 · see citing works

Improving cancer immunotherapy using nanomedicines: progress, opportunities and challenges

Martin, J. D., Cabral, H., Stylianopoulos, T., & Jain, R. K. (2020) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-019-0308-z · Cited by 688 · see citing works

Base editing: advances and therapeutic opportunities

Porto, E. M., Komor, A. C., Slaymaker, I. M., & Yeo, G. W.-M. (2020) · Nature Reviews Drug Discovery · doi:10.1038/s41573-020-0084-6 · Cited by 475 · see citing works

Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3

Qiu, M., Glass, Z., Chen, J., Haas, M., Jin, X., Zhao, X., Rui, X., Ye, Z., Li, Y., Zhang, F., et al. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2020401118 · Cited by 419 · see citing works

Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities

Li, L., Hu, S., & Chen, X. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.04.031 · Cited by 415 · see citing works

Nanotechnology in Plant Science: To Make a Long Story Short

Sanzari, I., Leone, A., & Ambrosone, A. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00120 · Cited by 383 · see citing works

Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles

Liu, J., Chang, J., Jiang, Y., Meng, X., Sun, T., Mao, L., Xu, Q., & Wang, M. (2019) · Advanced Materials · doi:10.1002/adma.201902575 · Cited by 373 · see citing works

Polymeric Delivery of Therapeutic Nucleic Acids

Kumar, R., Chalarca, C. F. S., Bockman, M. R., Bruggen, C. V., Grimme, C. J., Dalal, R. J., Hanson, M. G., Hexum, J. K., & Reineke, T. M. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00997 · Cited by 370 · see citing works

CRISPR/Cas-Mediated Base Editing: Technical Considerations and Practical Applications

Molla, K. A., & Yang, Y. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.03.008 · Cited by 336 · see citing works

DNA hydrogel-based gene editing and drug delivery systems

Mo, F., Jiang, K., Zhao, D. S., Wang, Y., Song, J., & Tan, W. (2020) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2020.07.018 · Cited by 306 · see citing works

Paving the Road for RNA Therapeutics

Dammes, N., & Peer, D. (2020) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2020.08.004 · Cited by 220 · see citing works

In vivo delivery of CRISPR-Cas9 therapeutics: Progress and challenges

Behr, M. P., Zhou, J., Xu, B., & Zhang, H. (2021) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2021.05.020 · Cited by 220 · see citing works

A review of emerging physical transfection methods for CRISPR/Cas9-mediated gene editing

Fajrial, A. K., He, Q., Wirusanti, N. I., Slansky, J. E., & Ding, X. (2020) · Theranostics · doi:10.7150/thno.43465 · Cited by 198 · see citing works

Strategies for nonviral nanoparticle‐based delivery of CRISPR/Cas9 therapeutics

Chen, F., Alphonse, M., & Liu, Q. (2020) · WIREs Nanomedicine and Nanobiotechnology · doi:10.1002/wnan.1609 · Cited by 197 · see citing works

Gene editing and CRISPR in the clinic: current and future perspectives

Hirakawa, M. P., Krishnakumar, R., Timlin, J. A., Carney, J. P., & Butler, K. S. (2020) · Bioscience Reports · doi:10.1042/bsr20200127 · Cited by 197 · see citing works

CRISPR-Cas for genome editing: Classification, mechanism, designing and applications

Bhatia, S. K., ., P., & Yadav, S. K. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.124054 · Cited by 165 · see citing works

Targeted homology-directed repair in blood stem and progenitor cells with CRISPR nanoformulations

Shahbazi, R., Sghia-Hughes, G., Reid, J. L., Kubek, S., Haworth, K. G., Humbert, O., Kiem, H., & Adair, J. E. (2019) · Nature Materials · doi:10.1038/s41563-019-0385-5 · Cited by 158 · see citing works

Delivery of CRISPR/Cas9 for therapeutic genome editing

Xu, X., Wan, T. Y., Xin, H., Li, D., Pan, H., Wu, J., & Yuan, P. (2019) · The Journal of Gene Medicine · doi:10.1002/jgm.3107 · Cited by 147 · see citing works

Spatiotemporal Control of CRISPR/Cas9 Function in Cells and Zebrafish using Light‐Activated Guide RNA

Zhou, W., Brown, W., Bardhan, A., Delaney, M., Ilk, A. S., Rauen, R. R., Kahn, S. I., Tsang, M., & Deiters, A. (2020) · Angewandte Chemie International Edition · doi:10.1002/anie.201914575 · Cited by 139 · see citing works

An Outlook on Global Regulatory Landscape for Genome-Edited Crops

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