The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types
Open accessAbstract
SUMMARY Stem-cell differentiation to desired lineages requires navigating alternating developmental paths often leading to unwanted cell-types. Hence comprehensive developmental roadmaps are crucial to channel stem-cell differentiation towards desired fates. To this end, here we map bifurcating lineage choices leading from pluripotency to twelve human mesodermal lineages, including bone, muscle and heart. We defined the extrinsic signals controlling each binary lineage decision, enabling us to logically block differentiation towards unwanted fates and rapidly steer pluripotent stem cells towards 80–99% pure human mesodermal lineages at most branchpoints. This strategy enabled the generation of human bone and heart progenitors that could engraft in respective in vivo models. Mapping stepwise chromatin and single-cell gene expression changes in mesoderm development uncovered somite segmentation, a previously-unobservable human embryonic event transiently marked by HOPX expression. Collectively this roadmap enables navigation of mesodermal development to produce transplantable human tissue progenitors and uncover developmental processes.
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Chemically defined generation of human cardiomyocytes
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Species-specific segmentation clock periods are due to differential biochemical reaction speeds
Matsuda, M., Hayashi, H., Garcia-Ojalvo, J., Yoshioka-Kobayashi, K., Kageyama, R., Yamanaka, Y., Ikeya, M., Toguchida, J., Alev, C., & Ebisuya, M. (2020) · Science · doi:10.1126/science.aba7668 · Cited by 254
A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages
Tchieu, J., Zimmer, B., Fattahi, F., Amin, S., Zeltner, N., Chen, S., & Studer, L. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2017.08.015 · Cited by 235
A Roadmap for Human Liver Differentiation from Pluripotent Stem Cells
Ang, L. T., Tan, A. K. Y., Autio, M. I., Goh, S. H., Choo, S. H., Lee, K. L., Tan, J., Pan, B., Lee, J. J. H., Lum, J. J., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.01.087 · Cited by 220
Self-citationZhang, J., Tao, R., Campbell, K. F., Carvalho, J. L., Ruiz, E. C., Kim, G. C., Schmuck, E. G., Raval, A. N., da Rocha, A. M., Herron, T. J., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-09831-5 · Cited by 201
Synthetic multistability in mammalian cells
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CellVGAE: an unsupervised scRNA-seq analysis workflow with graph attention networks
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Uzbas, F., Opperer, F., Sönmezer, C., Shaposhnikov, D., Sass, S., Krendl, C., Angerer, P., Theis, F. J., Mueller, N. S., & Drukker, M. (2019) · Genome Biology · doi:10.1186/s13059-019-1748-6 · Cited by 23
Metadata
- Authors
- Loh, K. M., Chen, A., Koh, P. W., Deng, T. Z., Sinha, R., Tsai, J. M., Barkal, A. A., Shen, K. Y., Jain, R., Morganti, R. M., Shyh-Chang, N., Fernhoff, N. B., George, B. M., Wernig, G., Salomon, R. E. A., Chen, Z., Vogel, H., Epstein, J. A., Kundaje, A., Talbot, W. S., Beachy, P. A., Ang, L. T., & Weissman, I. L.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2016-07
- Volume
- 166
- Issue
- 2
- Pages
- 451-467
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2461230718
- Cited by
- 489
- References
- 4
- FWCI
- 18.21
- Type
- journal article
- Language
- en
- Open access
- bronze
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
