Cited by 533Protein Degradation and InhibitorsUbiquitin and proteasome pathwaysCRISPR and Genetic Engineering

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A Method for the Acute and Rapid Degradation of Endogenous Proteins

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Clift, Dean, McEwan, William A., Labzin, Larisa I., Konieczny, Vera, Mogessie, Binyam, James, Leo C., Schuh, Melina·2017·Cell

DOI 10.1016/j.cell.2017.10.033PMC PMC5733393

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Abstract

Methods for the targeted disruption of protein function have revolutionized science and greatly expedited the systematic characterization of genes. Two main approaches are currently used to disrupt protein function: DNA knockout and RNA interference, which act at the genome and mRNA level, respectively. A method that directly alters endogenous protein levels is currently not available. Here, we present Trim-Away, a technique to degrade endogenous proteins acutely in mammalian cells without prior modification of the genome or mRNA. Trim-Away harnesses the cellular protein degradation machinery to remove unmodified native proteins within minutes of application. This rapidity minimizes the risk that phenotypes are compensated and that secondary, non-specific defects accumulate over time. Because Trim-Away utilizes antibodies, it can be applied to a wide range of target proteins using off-the-shelf reagents. Trim-Away allows the study of protein function in diverse cell types, including non-dividing primary cells where genome- and RNA-targeting methods are limited.

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Authors: Clift, D., McEwan, W. A., Labzin, L. I., Konieczny, V., Mogessie, B., James, L. C., & Schuh, M.
Affiliations: MRC Laboratory of Molecular Biology (GB)
Max Planck Institute for Biophysical Chemistry (DE)
Corresponding author: Clift, Dean
James, Leo C.
Schuh, Melina
ORCID: Clift, Dean
McEwan, William A.
Labzin, Larisa I.
Mogessie, Binyam
James, Leo C.
Schuh, Melina
Venue: Cell
Publisher: Elsevier BV
Published: 2017-12
Volume: 171
Issue: 7
Pages: 1692-1706.e18
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/j.cell.2017.10.033
PMC: PMC5733393
OpenAlex: W2769995426
Cited by: 531
References: 65
Citations by year: 2026: 65 · 2025: 85 · 2024: 59 · 2023: 72 · 2022: 52 · 2021: 62 · 2020: 58 · 2019: 45 · 2018: 30 · 2017: 2
FWCI: 15.00
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by/4.0/ (vor)
Locations: Cell (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Explore Bristol Research (publishedVersion, cc-by)
GRO (submittedVersion)
MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
Bristol Research (University of Bristol) (submittedVersion, cc-by)
Apollo (University of Cambridge) (submittedVersion, cc-by)
Apollo (cc-by)
Funders: Medical Research Council (MC_U105192711, MC_U105181010)
European Community’s Seventh Framework Programme (241548)
European Research Council (ERC (337415)
Wellcome Trust Investigator Award (200594/Z/16/Z)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Degradation and Inhibitors
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Keywords: mammalian cells, gene function analysis
MeSH terms: Animals, Antibodies, Biochemistry, Protein Transport, Proteolysis

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A Method for the Acute and Rapid Degradation of Endogenous Proteins

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