The DOI matches its Crossref record; 345 of the 345 references it cites were found in a registry or an index.
Past, present, and future of CRISPR genome editing technologies
Open accessAbstract
Genome editing has been a transformative force in the life sciences and human medicine, offering unprecedented opportunities to dissect complex biological processes and treat the underlying causes of many genetic diseases. CRISPR-based technologies, with their remarkable efficiency and easy programmability, stand at the forefront of this revolution. In this Review, we discuss the current state of CRISPR gene editing technologies in both research and therapy, highlighting limitations that constrain them and the technological innovations that have been developed in recent years to address them. Additionally, we examine and summarize the current landscape of gene editing applications in the context of human health and therapeutics. Finally, we outline potential future developments that could shape gene editing technologies and their applications in the coming years.
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In the citing paper
“Additionally, chemical modifications involving 2′-O-methyl or 2′-fluoro nucleotides and phosphorothioate linkages in the gRNA have proven to be effective strategies for enhancing both the specificity of Cas9 and the stability of the gRNA (Martin et al., 2024).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Martin et al., 2024
In the citing paper
“Nevertheless, each of the high-fidelity variants is not universally acceptable and possesses certain limitations (Martin et al., 2024).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Martin et al., 2024
In the citing paper
“The Cas12a does not require a tracrRNA for activation and is potentially exploited for multiplexed editing (Martin et al., 2024).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Martin et al., 2024
In the citing paper
“The first-generation CRISPR-based gene editing tools face several challenges, including specificity, targeting scope, dependency on endogenous DSB repair mechanisms, absence of efficient delivery methods, lack of effective vectors and rigidity of cell or organisms, off-target activity, etc. (Kadam et al., 2018; Martin et al., 2024).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Martin et al., 2024
In the citing paper
“Further base editing option has been expanded beyond ABE and CBE and covers A-to-C, A-to-Y and C-to-G (Martin et al., 2024) edits.”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Martin et al., 2024
In the citing paper
“Besides controlled editing, BEs are facing several limitations, including low efficiency, large editing windows, and off-target activity (Martin et al., 2024).”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Martin et al., 2024
Citations
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D. A., & Horvath, P. (2007) · Science · doi:10.1126/science.1138140 · Cited by 5,947
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437
Genome-scale CRISPR-Cas9 knockout screening in human cells
Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., et al. (2014) · Science · doi:10.1126/science.1247005 · Cited by 5,234
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016 · cited 1×
“196 To suppress gene expression in eukaryotic cells, nuclease-inactive Cas9 can be fused to various transcriptional and epigenetic modulators, for example, the KRAB transcriptional repressor domain, 197,198 and targeted to promoter regions of actively transcribed genes.”
BackgroundSearch-and-replace genome editing without double-strand breaks or donor DNA
Anzalone, A. V., Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., et al. (2019) · Nature · doi:10.1038/s41586-019-1711-4 · Cited by 4,767
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761
Cited by
Sorted by citations
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 55
Efficient Multiplex Genome Editing of the Cyanobacterium Synechocystis sp. PCC6803 via CRISPR‐Cas12a
Du, W., Meister, L. L., van Grinsven, T., & Branco dos Santos, F. (2025) · Biotechnology and Bioengineering · doi:10.1002/bit.28910 · Cited by 7
Metadata
- Authors
- Pacesa, M., Pelea, O., & Jinek, M.
- Affiliations
- University of Zurich (CH)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2024-02
- Volume
- 187
- Issue
- 5
- Pages
- 1076-1100
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W4392303996
- Cited by
- 609
- References
- 345
- Citations by year
- 2026: 266 · 2025: 262 · 2024: 81
- FWCI
- 24.90
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cell (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion, cc-by-nc-nd)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion, cc-by)
- Zurich Open Repository and Archive (University of Zurich)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- CRISPR gene editing, genome editing, CRISPR/Cas9, gene therapy, genetic disorders
- MeSH terms
- Gene Editing, Biological Science Disciplines, Humans, Technology, Genetic Therapy, CRISPR-Cas Systems
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