The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
T Lymphocyte Potential Marks the Emergence of Definitive Hematopoietic Progenitors in Human Pluripotent Stem Cell Differentiation Cultures
Open accessAbstract
The efficient generation of hematopoietic stem cells from human pluripotent stem cells is dependent on the appropriate specification of the definitive hematopoietic program during differentiation. In this study, we used T lymphocyte potential to track the onset of definitive hematopoiesis from human embryonic and induced pluripotent stem cells differentiated with specific morphogens in serum- and stromal-free cultures. We show that this program develops from a progenitor population with characteristics of hemogenic endothelium, including the expression of CD34, VE-cadherin, GATA2, LMO2, and RUNX1. Along with T cells, these progenitors display the capacity to generate myeloid and erythroid cells. Manipulation of Activin/Nodal signaling during early stages of differentiation revealed that development of the definitive hematopoietic progenitor population is not dependent on this pathway, distinguishing it from primitive hematopoiesis. Collectively, these findings demonstrate that it is possible to generate T lymphoid progenitors from pluripotent stem cells and that this lineage develops from a population whose emergence marks the onset of human definitive hematopoiesis.
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Kroon, E., Martinson, L. A., Kadoya, K., Bang, A. G., Kelly, O. G., Eliazer, S., Young, H., Richardson, M., Smart, N. G., Cunningham, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1393 · Cited by 1,843 · cited 2×
“…Wnt pathways all play a role in the early induction steps in hPSC cultures, including primitive streak formation, mesoderm induction, and hematopoietic specification (Davis et al., 2008; Jackson et al., 2010; Kennedy et al., 2007; Kroon et al., 2008; Sumi et al., 2008; Vijayaragavan et al., 2009).”
BackgroundOf lineage and legacy: the development of mammalian hematopoietic stem cells
Dzierzak, E., & Speck, N. A. (2008) · Nature Immunology · doi:10.1038/ni1560 · Cited by 707
Autonomous murine T-cell progenitor production in the extra-embryonic yolk sac before HSC emergence
Yoshimoto, M., Porayette, P., Glosson, N. L., Conway, S. J., Carlesso, N., Cardoso, A. A., Kaplan, M. H., & Yoder, M. C. (2012) · Blood · doi:10.1182/blood-2011-12-397489 · Cited by 178
Molecular Identity of Hematopoietic Precursor Cells Emerging in the Human Embryo
Labastie, M.-C., Cortés, F., Roméo, P.-H., Dulac, C., & Péault, B. (1998) · Blood · doi:10.1182/blood.v92.10.3624 · Cited by 153
Cited by
Themeli, M., Kloss, C. C., Ciriello, G., Fedorov, V. D., Perna, F., Gonen, M., & Sadelain, M. (2013) · Nature Biotechnology · doi:10.1038/nbt.2678 · Cited by 473
Sturgeon, C. M., Ditadi, A., Awong, G., Kennedy, M., & Keller, G. (2014) · Nature Biotechnology · doi:10.1038/nbt.2915 · Cited by 467
Self-citationReprogramming Committed Murine Blood Cells to Induced Hematopoietic Stem Cells with Defined Factors
Riddell, J., Gazit, R., Garrison, B. S., Guo, G., Saadatpour, A., Mandal, P. K., Ebina, W., Volchkov, P., Yuan, G.-C., Orkin, S. H., et al. (2014) · Cell · doi:10.1016/j.cell.2014.04.006 · Cited by 323
Ditadi, A., Sturgeon, C. M., Tober, J., Awong, G., Kennedy, M., Yzaguirre, A. D., Azzola, L., Ng, E. S., Stanley, E. G., French, D. L., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3161 · Cited by 320
Self-citationLachmann, N., Ackermann, M., Frenzel, E., Liebhaber, S., Brennig, S., Happle, C., Hoffmann, D., Klimenkova, O., Lüttge, D., Buchegger, T., et al. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.01.005 · Cited by 238
Metadata
- Authors
- Kennedy, M., Awong, G., Sturgeon, C. M., Ditadi, A., LaMotte-Mohs, R., Zúñiga-Pflücker, J. C., & Keller, G.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2012-12
- Volume
- 2
- Issue
- 6
- Pages
- 1722-1735
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2155733789
- Cited by
- 400
- References
- 4
- FWCI
- 9.99
- Type
- journal article
- Language
- en
- Open access
- gold
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