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Proliferation Drives Aging-Related Functional Decline in a Subpopulation of the Hematopoietic Stem Cell Compartment

Kirschner, Kristina, Chandra, Tamir, Kiselev, Vladimir, Flores-Santa Cruz, David, Macaulay, Iain C., Park, Hyun Jun, Li, Juan, Kent, David G., Kumar, Rupa, Pask, Dean C., Hamilton, Tina L., Hemberg, Martin, Reik, Wolf, Green, Anthony R.·2017·Cell Reports
DOI 10.1016/j.celrep.2017.04.074PMC PMC5457484
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Figure 1 LT-HSCs Display a Distinct Subpopulation with Age (A) Sorting strategy for HSCs. (B) SC3 clustering of young and old HSC transcriptomes. Replicates: purple and green bars. Age: orange (young) and turquoise (old) bars. Similarity between cells is indicated from blue to red (identical). Rep, replicate. (C) Heatmap of top ten marker genes of cluster 1. Expression is shown from blue (low) to red (high). (D) Silhouette plots for all clusters. The silhouette index (si) and the number of cells per cluster are given. xSi, average silhouette index. (E) Boxplots for ESLAM marker intensity for old-specific (blue) and other old (red) HSCs. Rel. Expr., relative expression. (F) SCDE plots for marker genes of cluster 1 in young (blue) and old (orange) HSCs. Expression levels of individual cells are indicated by individual lines. See also Supplemental Experimental Procedures.
Figure 1 LT-HSCs Display a Distinct Subpopulation with Age (A) Sorting strategy for HSCs. (B) SC3 clustering of young and old HSC transcriptomes. Replicates: purple and green bars. Age: orange (young) and turquoise (old) bars. Similarity between cells is indicated from blue to red (identical). Rep, replicate. (C) Heatmap of top ten marker genes of cluster 1. Expression is shown from blue (low) to red (high). (D) Silhouette plots for all clusters. The silhouette index (si) and the number of cells per cluster are given. xSi, average silhouette index. (E) Boxplots for ESLAM marker intensity for old-specific (blue) and other old (red) HSCs. Rel. Expr., relative expression. (F) SCDE plots for marker genes of cluster 1 in young (blue) and old (orange) HSCs. Expression levels of individual cells are indicated by individual lines. See also Supplemental Experimental Procedures.CC BY · PubMed Central
Figure 2 Age-Specific Cluster Carries Signature of Pro-proliferative and Anti-proliferative Stimuli (A) Manual annotation of top 20 marker genes with Ras senescence (RIS), apoptosis (Kirschner et al., 2015), and pStat3 and pStat5 datasets (Lau et al., 2015). Red indicates p53, and green indicates pStat targets. (B) KEGG pathway analysis of marker genes for old-specific cluster. Selected pathways are shown as ratio of enrichment (green indicates pro-proliferative, and red indicates anti-proliferative). (C) GSEA for p53 RIS, apoptosis (Apo), and STAT3 and STAT5 targets. p values and enrichment scores are shown. (D) Schematic of TPO-regulated microarray experiment in HPC7. GSEA of TPO-specific targets and marker gene list. (E) IF quantification of old HSCs for p53Ser15 and/or gH2AX (n = 3; error bars indicate SEM). (F) Quantification of EdU (5-ethynyl-2′-deoxyuridine) incorporation, gH2AX, and p53 in old HSCs (n = 3; error bars indicates SEM). (G) GSEA for lineage markers. ChIP, chromatin immunoprecipitation, MA, microarray; TGF, transforming growth factor; n.s., not significant; nb, number of genes.
Figure 2 Age-Specific Cluster Carries Signature of Pro-proliferative and Anti-proliferative Stimuli (A) Manual annotation of top 20 marker genes with Ras senescence (RIS), apoptosis (Kirschner et al., 2015), and pStat3 and pStat5 datasets (Lau et al., 2015). Red indicates p53, and green indicates pStat targets. (B) KEGG pathway analysis of marker genes for old-specific cluster. Selected pathways are shown as ratio of enrichment (green indicates pro-proliferative, and red indicates anti-proliferative). (C) GSEA for p53 RIS, apoptosis (Apo), and STAT3 and STAT5 targets. p values and enrichment scores are shown. (D) Schematic of TPO-regulated microarray experiment in HPC7. GSEA of TPO-specific targets and marker gene list. (E) IF quantification of old HSCs for p53Ser15 and/or gH2AX (n = 3; error bars indicate SEM). (F) Quantification of EdU (5-ethynyl-2′-deoxyuridine) incorporation, gH2AX, and p53 in old HSCs (n = 3; error bars indicates SEM). (G) GSEA for lineage markers. ChIP, chromatin immunoprecipitation, MA, microarray; TGF, transforming growth factor; n.s., not significant; nb, number of genes.CC BY · PubMed Central
Figure 3 Constitutively Active JAK2 Increases Cell Contribution to the Old-Specific Subpopulation (A) Kinetics of JAK2V617F and WT HSCs. Ratios of JAK2V617F and WT for indicated divisions in young (black) and old (gray) HSCs. Black line denotes WT levels (n = 3; error bars indicate SEM). Mut, mutated. (B) Kinetics data from young (upper row) and old (lower row) WT (blue) and homozygous (Hom) JAK2V671F (red) HSCs. Percentage of all cells upon division (n = 3; error bars indicate SEM). hrs, hours. (C) SC3 clustering of HSC transcriptomes from young (yg; upper orange bars) and old (upper turquoise bars) WT (lower orange bars) and JAK2V617F (lower turquoise bars) mice. Replicates are indicated in green and red. Similarity between cells is indicated from blue to red (identical). (D) SC3 clustering of old HSC transcriptomes from WT and JAK2V617F mice (replicate 1). Overlap with marker genes is given as a percentage. Cell number for old-specific HSCs is given as a percentage of all old HSCs. (E) Immunofluorescent images of old WT and JAK2V617F HSCs stained for pSer15p53 (green), pSer27FosB (red), and DAPI (blue). Bar plots quantify the percentage of double-positive HSCs from indicated mice (n = 3; error bars indicate SEM). (F) SC3 clustering of 1-year-old WT and JAK2V617F HSCs. Similarity between cells is indicated from blue to red (identical). Overlap with marker genes is shown in percentages. (G) Model for relation between proliferation and functional decline of HSCs. Compared are physiological (upper row) against enforced proliferation through JAK2V617F (lower row). Young HSCs in the JAK2 condition expand faster (number of cells) but exhaust more readily upregulating p53- and cyclin-dependent kinase inhibitors (CDKNs; shades of red). Arrow cycle indicates self-renewal.
Figure 3 Constitutively Active JAK2 Increases Cell Contribution to the Old-Specific Subpopulation (A) Kinetics of JAK2V617F and WT HSCs. Ratios of JAK2V617F and WT for indicated divisions in young (black) and old (gray) HSCs. Black line denotes WT levels (n = 3; error bars indicate SEM). Mut, mutated. (B) Kinetics data from young (upper row) and old (lower row) WT (blue) and homozygous (Hom) JAK2V671F (red) HSCs. Percentage of all cells upon division (n = 3; error bars indicate SEM). hrs, hours. (C) SC3 clustering of HSC transcriptomes from young (yg; upper orange bars) and old (upper turquoise bars) WT (lower orange bars) and JAK2V617F (lower turquoise bars) mice. Replicates are indicated in green and red. Similarity between cells is indicated from blue to red (identical). (D) SC3 clustering of old HSC transcriptomes from WT and JAK2V617F mice (replicate 1). Overlap with marker genes is given as a percentage. Cell number for old-specific HSCs is given as a percentage of all old HSCs. (E) Immunofluorescent images of old WT and JAK2V617F HSCs stained for pSer15p53 (green), pSer27FosB (red), and DAPI (blue). Bar plots quantify the percentage of double-positive HSCs from indicated mice (n = 3; error bars indicate SEM). (F) SC3 clustering of 1-year-old WT and JAK2V617F HSCs. Similarity between cells is indicated from blue to red (identical). Overlap with marker genes is shown in percentages. (G) Model for relation between proliferation and functional decline of HSCs. Compared are physiological (upper row) against enforced proliferation through JAK2V617F (lower row). Young HSCs in the JAK2 condition expand faster (number of cells) but exhaust more readily upregulating p53- and cyclin-dependent kinase inhibitors (CDKNs; shades of red). Arrow cycle indicates self-renewal.CC BY · PubMed Central

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Citations

Cites

Research works printed but not linked: 22

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Metadata

Authors
Kirschner, K., Chandra, T., Kiselev, V., Flores-Santa Cruz, D., Macaulay, I. C., Park, H. J., Li, J., Kent, D. G., Kumar, R., Pask, D. C., Hamilton, T. L., Hemberg, M., Reik, W., & Green, A. R.
Venue
Cell Reports
Publisher
Elsevier BV
Published
2017-05
Volume
19
Issue
8
Pages
1503-1511
ISSN
2211-1247
DOI
10.1016/j.celrep.2017.04.074
PMC
PMC5457484
Cited by
100
References
12
Language
en
Licence
Funders
  • NIHR Cambridge Biomedical Research Centre
  • Wellcome Trust (095645/Z/11/Z)
  • Medical Research Council
  • Biotechnology and Biological Sciences Research Council (BB/K010867/1)
  • Kay Kendall Leukaemia Fund
  • European Commission (104710/Z/14/Z, 13003)

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