An mRNA Vaccine Protects Mice against Multiple Tick-Transmitted Flavivirus Infections
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Powassan virus (POWV) is an emerging tick-transmitted flavivirus that circulates in North America and Russia. Up to 5% of deer ticks now test positive for POWV in certain regions of the northern United States. Although POWV infections cause life-threatening encephalitis, there is no vaccine or countermeasure available for prevention or treatment. Here, we developed a lipid nanoparticle (LNP)-encapsulated modified mRNA vaccine encoding the POWV prM and E genes and demonstrated its immunogenicity and efficacy in mice following immunization with one or two doses. The POWV mRNA vaccine induced high titers of neutralizing antibody and sterilizing immunity against lethal challenge with different POWV strains. The mRNA vaccine also induced cross-neutralizing antibodies against multiple other tick-borne flaviviruses and protected mice against the distantly related Langat virus. These data demonstrate the utility of the LNP-mRNA vaccine platform for the development of vaccines with protective activity against multiple flaviviruses.
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Lipid nanoparticle-mRNA vaccines have been tested against other viruses in animal models, including human immunodeficiency virus, Powassan virus, Venezuelan equine encephalitis virus, dengue virus and Ebola virus.
“Furthermore, lipid nanoparticle–mRNA vaccines have been tested against other viruses in animal models, including human immunodeficiency virus28,144,181,190, Powassan virus195, Venezuelan equine encephalitis virus196, dengue virus73 and Ebola virus197.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [195]
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- Authors
- VanBlargan, L. A., Himansu, S., Foreman, B. M., Ebel, G. D., Pierson, T. C., & Diamond, M. S.
- Affiliations
- Washington University in St. Louis (US)
- National Institutes of Health (US)
- Colorado State University (US)
- Corresponding author
- Diamond, Michael S.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2018-12
- Volume
- 25
- Issue
- 12
- Pages
- 3382-3392.e3
- ISSN
- 2211-1247 (ISSN-L)
- PMC
- PMC6353567
- OpenAlex
- W2903666247
- Cited by
- 123
- References
- 57
- Citations by year
- 2026: 7 · 2025: 16 · 2024: 23 · 2023: 23 · 2022: 24 · 2021: 14 · 2020: 14 · 2019: 2
- FWCI
- 7.14
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Digital Commons@Becker (Washington University School of Medicine) (submittedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- NIH-NIAID (R01 AI073755)
- Moderna
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
- Health Sciences › Medicine › Infectious Diseases › Viral Infections and Vectors
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Keywords
- Powassan virus, mRNA vaccines, lipid nanoparticles, tick-borne flaviviruses, Langat virus, cross-neutralizing antibodies, sterilizing immunity, Ixodes scapularis, encephalitis, vaccine immunogenicity
- MeSH terms
- Animals, Disease Models, Animal, Encephalitis Viruses, Tick-Borne, Lipids, Mice, Inbred C57BL, Neutralization Tests, Phylogeny, RNA, Messenger, Vaccination, Viral Vaccines, Flavivirus Infections, Ixodes, Nanoparticles
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