Characterization of stem-cell-derived islets during differentiation and after implantation
Open accessAbstract
Recapitulation of embryonic pancreatic development has enabled development of methods for in vitro islet cell differentiation using human pluripotent stem cells (hPSCs), which have the potential to cure diabetes. Advanced methods for optimal generation of stem-cell-derived islets (SC-islets) has enabled successful diabetes reversal in rodents and shown promising early clinical trial outcomes. The main impediment for use of SC-islets is concern about safety because of off-target growth resulting from contaminated residual cells. In this review, we summarize the different endocrine and non-endocrine cell populations that have been described to emerge throughout β cell differentiation and after transplantation. We discuss the most recent approaches to enrich endocrine populations and remove off-target cells. Finally, we discuss the critical quality control and release criteria testing that we anticipate will be required prior to transplantation to ensure product safety.
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With less optimized protocols or cell lines, there is potential to retain progenitor cells in the final islet clusters as well as produce other endodermal cell types, such as those of hepatic origin.
“With less optimized protocols or cell lines, there is also the potential to retain progenitor cells in the final islet clusters as well as produce other endodermal cell types, such as those of hepatic origin.39,58”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [58]
The Introduction (page unavailable) says stem-cell-derived islets can retain progenitors and describes non-pancreatic endodermal tissues arising from contaminating cells, particularly gastrointestinal and respiratory epithelia. It does not identify cells of hepatic origin, so that example is not supported by this source. (LLM, conf=0.86)
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Islet size and spatial organization govern endocrine cell composition throughout the human pancreasPreprint
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- Authors
- Cuesta-Gomez, N., Verhoeff, K., Jasra, I. T., Pawlick, R., Dadheech, N., & Shapiro, A. M. J.
- Affiliations
- University of Alberta (CA)
- Corresponding author
- Dadheech, Nidheesh
- Shapiro, A.M. James
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2022-08
- Volume
- 40
- Issue
- 8
- Pages
- 111238
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W4292755723
- Cited by
- 41
- References
- 75
- Citations by year
- 2026: 9 · 2025: 12 · 2024: 10 · 2023: 9 · 2022: 1
- FWCI
- 1.91
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Canada Research Chairs
- Alberta Diabetes Foundation
- Juvenile Diabetes Research Foundation Canada
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- stem cell-derived islets, human pluripotent stem cells, islet cell differentiation, pancreatic development, beta cell differentiation, islet transplantation, diabetes mellitus, quality control, cell therapy safety
- MeSH terms
- Cell Differentiation, Humans, Islets of Langerhans, Pancreas, Islets of Langerhans Transplantation, Pluripotent Stem Cells
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