Acquisition of Dynamic Function in Human Stem Cell-Derived β Cells
Open accessAbstract
Recent advances in human pluripotent stem cell (hPSC) differentiation protocols have generated insulin-producing cells resembling pancreatic β cells. While these stem cell-derived β (SC-β) cells are capable of undergoing glucose-stimulated insulin secretion (GSIS), insulin secretion per cell remains low compared with islets and cells lack dynamic insulin release. Herein, we report a differentiation strategy focused on modulating transforming growth factor β (TGF-β) signaling, controlling cellular cluster size, and using an enriched serum-free media to generate SC-β cells that express β cell markers and undergo GSIS with first- and second-phase dynamic insulin secretion. Transplantation of these cells into mice greatly improves glucose tolerance. These results reveal that specific time frames for inhibiting and permitting TGF-β signaling are required during SC-β cell differentiation to achieve dynamic function. The capacity of these cells to undergo GSIS with dynamic insulin release makes them a promising cell source for diabetes cellular therapy.
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Transplantation of SC-islets into mice improves their insulin secretion and glucose responsiveness, suggesting that SC-islet immaturity can be resolved.
“Transplantation of SC-islets into mice improves their insulin secretion and glucose responsiveness, suggesting that SC-islet immaturity can be resolved.”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [42]
The Results section, “Transplantation of SC-β Cells into Glucose-Intolerant Mice” (page unavailable), reports glucose-responsive human insulin secretion after transplantation and improved glucose tolerance in recipient mice. But it does not show that transplantation improved the cells’ insulin secretion or glucose responsiveness relative to before transplantation. The Discussion instead attributes enhanced functional maturation to the six-stage differentiation strategy, so the claim that transplantation resolves immaturity is not supported. (LLM, conf=0.86)
Experimental — accuracy not yet measuredThese SC-β cells have been shown to improve glycemic control in diabetic mice.
“These cells have been shown to improve glycemic control in diabetic mice, highlighting their potential utility in vivo.36,37,39–44”
From Developments in stem cell-derived islet replacement therapy for treating type 1 diabetes (2023) · cites this paper as [42]
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Zhang, Q., Wang, C., Zheng, M., Li, Y., Li, J., Zhang, L., Shang, X., & Yan, C. (2014) · Cerebrovascular Diseases · doi:10.1159/000369778 · Cited by 11,609 · see citing works
Projections of Global Mortality and Burden of Disease from 2002 to 2030
Mathers, C. D., & Loncar, D. (2006) · PLoS Medicine · doi:10.1371/journal.pmed.0030442 · Cited by 11,386 · see citing works
Shapiro, A. M. J., Lakey, J. R. T., Ryan, E. A., Korbutt, G. S., Toth, E., Warnock, G. L., Kneteman, N. M., & Rajotte, R. V. (2000) · New England Journal of Medicine · doi:10.1056/nejm200007273430401 · Cited by 5,237 · see citing works
Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas
Lacy, P. E., & Kostianovsky, M. (1967) · Diabetes · doi:10.2337/diab.16.1.35 · Cited by 2,775 · see citing works
Generation of Functional Human Pancreatic β Cells In Vitro
Pagliuca, F. W., Millman, J. R., Gürtler, M., Segel, M., Van Dervort, A., Ryu, J. H., Peterson, Q. P., Greiner, D., & Melton, D. A. (2014) · Cell · doi:10.1016/j.cell.2014.09.040 · Cited by 2,025 · see citing works
Self-citationInternational trial of the Edmonton protocol for islet transplantation
Shapiro, A. M. J., Ricordi, C., Hering, B. J., Auchincloss, H., Lindblad, R., Robertson, R. P., Secchi, A., Brendel, M. D., Berney, T., Brennan, D. C., et al. (2006) · New England Journal of Medicine · doi:10.1056/nejmoa061267 · Cited by 1,942 · see citing works
Production of pancreatic hormone–expressing endocrine cells from human embryonic stem cells
D’Amour, K. A., Bang, A. G., Eliazer, S., Kelly, O., Agulnick, A. D., Smart, N. G., Moorman, M. A., KROON, E. J., Carpenter, M. K., & Baetge, E. E. (2006) · Nature Biotechnology · doi:10.1038/nbt1259 · Cited by 1,913 · see citing works
Kroon, E., Martinson, L. A., Kadoya, K., Bang, A. G., Kelly, O. G., Eliazer, S., Young, H., Richardson, M., Smart, N. G., Cunningham, J., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1393 · Cited by 1,843 · cited 2× · see citing works
“Through modulation of pathways identified from embryonic development, studies with hPSCs have detailed protocols for generating cells that resemble early endoderm and pancreatic progenitors (D’Amour et al., 2006; D’Amour et al., 2005; Kroon et al., 2008; Nostro et al., 2015; Rezania et al., 2012), the latter of which…”
BackgroundEfficient differentiation of human embryonic stem cells to definitive endoderm
D'Amour, K. A., Agulnick, A. D., Eliazer, S., Kelly, O. G., Kroon, E., & Baetge, E. E. (2005) · Nature Biotechnology · doi:10.1038/nbt1163 · Cited by 1,730 · see citing works
Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells
Rezania, A., Bruin, J. E., Arora, P., Rubin, A., Batushansky, I., Asadi, A., O'Dwyer, S., Quiskamp, N., Mojibian, M., Albrecht, T., et al. (2014) · Nature Biotechnology · doi:10.1038/nbt.3033 · Cited by 1,575 · see citing works
Baron, M., Veres, A., Wolock, S. L., Faust, A. L., Gaujoux, R., Vetere, A., Ryu, J. H., Wagner, B. K., Shen-Orr, S. S., Klein, A. M., et al. (2016) · Cell Systems · doi:10.1016/j.cels.2016.08.011 · Cited by 1,508 · see citing works
Long-Term Complications of Diabetes Mellitus
Nathan, D. M. (1993) · New England Journal of Medicine · doi:10.1056/nejm199306103282306 · Cited by 1,495 · see citing works
Zhang, C.-Y., Baffy, G., Perret, P., Krauss, S., Peroni, O., Grujic, D., Hagen, T., Vidal-Puig, A. J., Boss, O., Kim, Y.-B., et al. (2001) · Cell · doi:10.1016/s0092-8674(01)00378-6 · Cited by 917 · see citing works
Vegas, A. J., Veiseh, O., Gürtler, M., Millman, J. R., Pagliuca, F. W., Bader, A. R., Doloff, J. C., Li, J., Chen, M., Olejnik, K., et al. (2016) · Nature Medicine · doi:10.1038/nm.4030 · Cited by 710 · see citing works
Self-citationRNA Sequencing of Single Human Islet Cells Reveals Type 2 Diabetes Genes
Xin, Y., Kim, J., Okamoto, H., Ni, M., Wei, Y., Adler, C., Murphy, A. J., Yancopoulos, G. D., Lin, C., & Gromada, J. (2016) · Cell Metabolism · doi:10.1016/j.cmet.2016.08.018 · Cited by 677 · see citing works
Hering, B. J., Clarke, W. R., Bridges, N. D., Eggerman, T. L., Alejandro, R., Bellin, M. D., Chaloner, K., Czarniecki, C. W., Goldstein, J. S., Hunsicker, L. G., et al. (2016) · Diabetes Care · doi:10.2337/dc15-1988 · Cited by 645 · see citing works
Generation of pluripotent stem cells from patients with type 1 diabetes
Maehr, R., Chen, S.-B., Snitow, M. E., Ludwig, T., Yagasaki, L., Goland, R., Leibel, R., & Melton, D. (2009) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.0906894106 · Cited by 600 · see citing works
Controlled induction of human pancreatic progenitors produces functional beta‐like cells in vitro
Russ, H. A., Parent, A. V., Ringler, J. J., Hennings, T. G., Nair, G. G., Shveygert, M., Guo, T., Puri, S., Haataja, L., Cirulli, V., et al. (2015) · The EMBO Journal · doi:10.15252/embj.201591058 · Cited by 591 · see citing works
Rezania, A., Bruin, J. E., Riedel, M. J., Mojibian, M., Asadi, A., Xu, J., Gauvin, R., Narayan, K., Karanu, F., O’Neil, J. J., et al. (2012) · Diabetes · doi:10.2337/db11-1711 · Cited by 582 · see citing works
Insulin Independence After Islet Transplantation Into Type I Diabetic Patient
Scharp, D. W., Lacy, P. E., Santiago, J. V., McCullough, C. S., Weide, L. G., Falqui, L., Marchetti, P., Gingerich, R. L., Jaffe, A. S., Cryer, P. E., et al. (1990) · Diabetes · doi:10.2337/diab.39.4.515 · Cited by 413 · see citing works
New sources of pancreatic β-cells
Bonner‐Weir, S., & Weir, G. C. (2005) · Nature Biotechnology · doi:10.1038/nbt1115 · Cited by 390 · see citing works
Generation of stem cell-derived β-cells from patients with type 1 diabetes
Millman, J., Xie, C.-H., VanDervort, A. V., Gürtler, M., Pagliuca, F., & Melton, D. (2016) · Nature Communications · doi:10.1038/ncomms11463 · Cited by 375 · see citing works
Self-citationDynamics of insulin secretion and the clinical implications for obesity and diabetes
Seino, S., Shibasaki, T., & Minami, K. (2011) · Journal of Clinical Investigation · doi:10.1172/jci45680 · Cited by 364 · see citing works
Bellin, M. D., Barton, F. B., Heitman, A., Harmon, J. V., Kandaswamy, R., Balamurugan, A. N., Sutherland, D. E. R., Alejandro, R., & Hering, B. J. (2012) · American Journal of Transplantation · doi:10.1111/j.1600-6143.2011.03977.x · Cited by 350 · see citing works
Nostro, M., Sarangi, F., Yang, C.-X., Holland, A. M., Elefanty, A., Stanley, E., Greiner, D. L., & Keller, G. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.02.017 · Cited by 302 · see citing works
Differentiated human stem cells resemble fetal, not adult, β cells
Hrvatin, S., O’Donnell, C. W., Deng, F., Millman, J. R., Pagliuca, F. W., DiIorio, P., Rezania, A., Gifford, D. K., & Melton, D. A. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1400709111 · Cited by 302 · cited 2× · see citing works
“These polyhormonal cells are known to not to resemble adult b cells and are not functional (D’Amour et al., 2006; Hrvatin et al., 2014).”
BackgroundSelf-citationRezania, A., Bruin, J. E., Xu, J., Narayan, K., Fox, J., O'Neil, J., & Kieffer, T. (2013) · Stem Cells · doi:10.1002/stem.1489 · Cited by 269 · see citing works
Prato, S. D., & Tiengo, A. (2001) · Diabetes/Metabolism Research and Reviews · doi:10.1002/dmrr.198 · Cited by 237 · see citing works
Update on Islet Transplantation
McCall, M., & James Shapiro, A. M. (2012) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a007823 · Cited by 226 · see citing works
Age-Dependent Pancreatic Gene Regulation Reveals Mechanisms Governing Human β Cell Function
Arda, H. E., Li, L., Tsai, J., Torre, E. A., Rosli, Y., Peiris, H., Spitale, R. C., Dai, C., Gu, X., Qu, K., et al. (2016) · Cell Metabolism · doi:10.1016/j.cmet.2016.04.002 · Cited by 222 · see citing works
Lin, H., Lee, J.-H., Yadav, H., Kamaraju, A. K., Liu, E. V., Zhigang, D., Vieira, A., Kim, S., Collins, H. W., Matschinsky, F. M., et al. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m805379200 · Cited by 189 · see citing works
β-Cell Dysfunction Due to Increased ER Stress in a Stem Cell Model of Wolfram Syndrome
Shang, L.-S., Hua, H.-Q., Foo, K., Martinez, H., Watanabe, K., Zimmer, M., Kahler, D. J., Freeby, M., Chung, W., Leduc, C., et al. (2014) · Diabetes · doi:10.2337/db13-0717 · Cited by 188 · see citing works
First-phase insulin secretion: does it exist in real life? Considerations on shape and function
Caumo, A., & Luzi, L. (2004) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00139.2003 · Cited by 174 · see citing works
Stokes, A., & Preston, S. H. (2017) · PLoS ONE · doi:10.1371/journal.pone.0170219 · Cited by 167 · see citing works
Human pancreatic beta-like cells converted from fibroblasts
Zhu, S.-Y., Russ, H., Wang, X., Zhang, M., Ma, T.-H., Xu, T., Tang, S.-B., Hebrok, M., & Ding, S. (2016) · Nature Communications · doi:10.1038/ncomms10080 · Cited by 145 · see citing works
Reversal of β cell de-differentiation by a small molecule inhibitor of the TGFβ pathway
Blum, B., Roose, A. N., Barrandon, O., Maehr, R., Arvanites, A. C., Davidow, L. S., Davis, J. C., Peterson, Q. P., Rubin, L. L., & Melton, D. A. (2014) · eLife · doi:10.7554/elife.02809 · Cited by 133 · see citing works
Lyon, J., Fox, J. E. M., Spigelman, A., Kim, R., Smith, N., O'Gorman, D., Kin, T., Shapiro, A. M. J., Rajotte, R., & Macdonald, P. (2016) · Endocrinology · doi:10.1210/en.2015-1562 · Cited by 120 · see citing works
Inhibition of TGF-β Signaling Promotes Human Pancreatic β-Cell Replication
Dhawan, S., Dirice, E., Kulkarni, R. N., & Bhushan, A. (2016) · Diabetes · doi:10.2337/db15-1331 · Cited by 119 · see citing works
Teo, A., Windmueller, R., Johansson, B., Dirice, E., Njølstad, P., Tjora, E., Ræder, H., & Kulkarni, R. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.c112.428979 · Cited by 115 · see citing works
Zeng, H., Guo, M. A., Zhou, T., Tan, L., Chong, C. N., Zhang, T., Dong, X., Xiang, J., Yu, A. S., Yue, L., et al. (2016) · Cell stem cell · doi:10.1016/j.stem.2016.07.002 · Cited by 111 · see citing works
Tritschler, S., Theis, F. J., Lickert, H., & Böttcher, A. (2017) · Molecular Metabolism · doi:10.1016/j.molmet.2017.06.021 · Cited by 110 · see citing works
Autologous Pluripotent Stem Cell–Derived β-Like Cells for Diabetes Cellular Therapy
Millman, J., & Pagliuca, F. (2017) · Diabetes · doi:10.2337/db16-1406 · Cited by 97 · see citing works
Self-citationPetersen, M., Azad, A., Ingvorsen, C., Hess, K., Hansson, M., Grapin-Botton, A., & Honoré, C. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.08.009 · Cited by 96 · see citing works
ROCKII inhibition promotes the maturation of human pancreatic beta-like cells
Ghazizadeh, Z., Kao, D.-I., Amin, S., Cook, B. D., Rao, S. D., Zhou, T., Zhang, T., Xiang, Z.-Y., Kenyon, R., Kaymakcalan, O. E., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00129-y · Cited by 88 · see citing works
Kudva, Y. C., Ohmine, S., Greder, L. V., Dutton, J. R., Armstrong, A. S., Lamo, J. G. D., Khan, Y., Thatava, T., Hasegawa, M., Fusaki, N., et al. (2012) · Stem Cells Translational Medicine · doi:10.5966/sctm.2011-0044 · Cited by 87 · see citing works
Economic 3D-printing approach for transplantation of human stem cell-derived β -like cells
Song, J., & Millman, J. R. (2016) · Biofabrication · doi:10.1088/1758-5090/9/1/015002 · Cited by 86 · see citing works
Self-citationHuman islet preparations distributed for research exhibit a variety of insulin-secretory profiles
Kayton, N. S., Poffenberger, G., Henske, J. A., Dai, C., Thompson, C. S., Aramandla, R., Shostak, A., Nicholson, W. E., Briššová, M., Bush, W. S., et al. (2015) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00437.2014 · Cited by 83 · see citing works
β-Cell Replacement in Mice Using Human Type 1 Diabetes Nuclear Transfer Embryonic Stem Cells
Sui, L., Danzl, N., Campbell, S. R., Viola, R., Williams, D. J., Xing, Y., Wang, Y., Phillips, N., Poffenberger, G., Johannesson, B., et al. (2017) · Diabetes · doi:10.2337/db17-0120 · Cited by 79 · see citing works
Bruin, J. E., Asadi, A., Fox, J., Erener, S., Rezania, A., & Kieffer, T. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.10.013 · Cited by 72 · see citing works
Discovery of a drug candidate for GLIS3-associated diabetes
Amin, S., Cook, B. D., Zhou, T., Ghazizadeh, Z., Lis, R., Zhang, T., Khalaj, M., Crespo, M., Perera, M., Xiang, J., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-04918-x · Cited by 68 · see citing works
Stem cell approaches for diabetes: towards beta cell replacement
Weir, G., Cavelti-Weder, C., & Bonner-Weir, S. (2011) · Genome Medicine · doi:10.1186/gm277 · Cited by 65 · see citing works
Development of an Encapsulated Stem Cell-Based Therapy for Diabetes
Tomei, A., Villa, C., & Ricordi, C. (2015) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2015.1055242 · Cited by 62 · see citing works
Simsek, S., Zhou, T., Robinson, C. L., Tsai, S., Crespo, M., Amin, S., Lin, X., Hon, J. D., Evans, T., & Chen, S. (2016) · Stem Cells Translational Medicine · doi:10.5966/sctm.2015-0276 · Cited by 57 · see citing works
Lineage determinants in early endocrine development
Rieck, S., Bankaitis, E., & Wright, C. (2012) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2012.06.005 · Cited by 56 · see citing works
Stimulation of insulin secretion by transforming growth factor- β
Totsuka, Y., Tabuchi, M., Kojima, I., Eto, Y., Shibai, H., & Ogata, E. (1989) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(89)92829-5 · Cited by 36 · see citing works
Detailed protocol for evaluation of dynamic perifusion of human islets to assess β-cell function
Bentsi-Barnes, K., Doyle, M. E., Abad, D., KANDEEL, F. R., & Al-Abdullah, I. H. (2011) · Islets · doi:10.4161/isl.3.5.15938 · Cited by 29 · see citing works
Flagged first, then by citations
1 of the 57 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Single‐cell RNA sequencing technologies and applications: A brief overview
Jovic, D., Liang, X., Zeng, H., Lin, L., Xu, F., & Luo, Y. (2022) · Clinical and Translational Medicine · doi:10.1002/ctm2.694 · Cited by 1,307 · see citing works
Balboa, D., Barsby, T., Lithovius, V., Saarimäki-Vire, J., Omar-Hmeadi, M., Dyachok, O., Montaser, H., Lund, P.-E., Yang, M., Ibrahim, H., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01219-z · Cited by 433 · see citing works
Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells
Hogrebe, N. J., Augsornworawat, P., Maxwell, K. G., Velazco-Cruz, L., & Millman, J. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0430-6 · Cited by 373 · see citing works
Self-citationGeneration of insulin-producing pancreatic β cells from multiple human stem cell lines
Hogrebe, N. J., Maxwell, K. G., Augsornworawat, P., & Millman, J. R. (2021) · Nature Protocols · doi:10.1038/s41596-021-00560-y · Cited by 211 · see citing works
Self-citationDevelopments in stem cell-derived islet replacement therapy for treating type 1 diabetes
Hogrebe, N. J., Ishahak, M., & Millman, J. R. (2023) · Cell Stem Cell · doi:10.1016/j.stem.2023.04.002 · Cited by 210 · see citing works
Self-citationAugsornworawat, P., Maxwell, K. G., Velazco-Cruz, L., & Millman, J. R. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108067 · Cited by 200 · see citing works
Self-citationChiou, J., Zeng, C., Zhang, C., Han, J. Y., Schlichting, M., Miller, M., Mendez, R., Huang, S., Wang, J., Sui, Y., et al. (2021) · Nature Genetics · doi:10.1038/s41588-021-00823-0 · Cited by 175 · see citing works
Maxwell, K. G., Augsornworawat, P., Velazco-Cruz, L., Kim, M. H., Asada, R., Hogrebe, N. J., Morikawa, S., Urano, F., & Millman, J. R. (2020) · Science Translational Medicine · doi:10.1126/scitranslmed.aax9106 · Cited by 183 · see citing works
Self-citationDiagnosis and treatment of type 1 diabetes at the dawn of the personalized medicine era
Akil, A. A., Yassin, E., Al-Maraghi, A., ALIYEV, E. E., Al-Malki, K., & Fakhro, K. A. (2021) · Journal of Translational Medicine · doi:10.1186/s12967-021-02778-6 · Cited by 184 · see citing works
Mapping cells through time and space with moscot
Klein, D., Palla, G., Lange, M., Klein, M., Piran, Z., Gander, M., Meng-Papaxanthos, L., Sterr, M., Saber, L., Jing, C., et al. (2025) · Nature · doi:10.1038/s41586-024-08453-2 · Cited by 202 · see citing works
Emerging routes to the generation of functional β-cells for diabetes mellitus cell therapy
Nair, G. G., Tzanakakis, E. S., & Hebrok, M. (2020) · Nature Reviews Endocrinology · doi:10.1038/s41574-020-0375-3 · Cited by 134 · see citing works
Stem Cell-Based Clinical Trials for Diabetes Mellitus
de Klerk, E., & Hebrok, M. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.631463 · Cited by 111 · see citing works
Applications of iPSC-derived beta cells from patients with diabetes
Maxwell, K. G., & Millman, J. (2021) · Cell Reports Medicine · doi:10.1016/j.xcrm.2021.100238 · Cited by 118 · see citing works
Self-citationGlucose Response by Stem Cell-Derived β Cells In Vitro Is Inhibited by a Bottleneck in Glycolysis
Davis, J. C., Alves, T., Helman, A., Chen, J. C., Kenty, J. H. R., Cardone, R., Liu, D. R., Kibbey, R., & Melton, D. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107623 · Cited by 105 · see citing works
Insights into pancreatic islet cell dysfunction from type 2 diabetes mellitus genetics
Krentz, N. A. J., & Gloyn, A. L. (2020) · Nature Reviews Endocrinology · doi:10.1038/s41574-020-0325-0 · Cited by 128 · see citing works
Engineering islets from stem cells for advanced therapies of diabetes
Siehler, J., Blöchinger, A. K., Meier, M., & Lickert, H. (2021) · Nature Reviews Drug Discovery · doi:10.1038/s41573-021-00262-w · Cited by 125 · see citing works
Latres, E., Finan, D. A., Greenstein, J. L., Kowalski, A. J., & Kieffer, T. J. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.02.007 · Cited by 118 · see citing works
Wang, X., Maxwell, K. G., Wang, K., Bowers, D. T., Flanders, J. A., Liu, W., Wang, L.-H., Liu, Q., Liu, C., Naji, A., et al. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.abb4601 · Cited by 119 · see citing works
Self-citationAdvances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes
Cayabyab, F., Nih, L. R., & Yoshihara, E. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.732431 · Cited by 119 · see citing works
Zhan, L., Rao, J. S., Sethia, N., Slama, M. Q., Han, Z., Tobolt, D., Etheridge, M., Peterson, Q. P., Dutcher, C. S., Bischof, J. C., et al. (2022) · Nature Medicine · doi:10.1038/s41591-022-01718-1 · Cited by 115 · see citing works
Generation of pancreatic β cells from CD177+ anterior definitive endoderm
Mahaddalkar, P. U., Scheibner, K., Pfluger, S., Ansarullah, Sterr, M., Beckenbauer, J., Irmler, M., Beckers, J., Knöbel, S., & Lickert, H. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0492-5 · Cited by 115 · see citing works
Parent, A. V., Faleo, G., Chavez, J., Saxton, M., Berrios, D. I., Kerper, N. R., Tang, Q., & Hebrok, M. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109538 · Cited by 96 · see citing works
Primary cilia control glucose homeostasis via islet paracrine interactions
Hughes, J. W., Cho, J. H., Conway, H., DiGruccio, M. R., Ng, X. W., Roseman, H. F., Abreu, D., Urano, F., & Piston, D. W. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2001936117 · Cited by 86 · see citing works
Cell Maturation: Hallmarks, Triggers, and Manipulation
Alvarez-Dominguez, J., & Melton, D. (2021) · Cell · doi:10.1016/j.cell.2021.12.012 · Cited by 78 · see citing works
Zbinden, A., Marzi, J., Schlünder, K., Probst, C., Urbanczyk, M., Black, S., Brauchle, E., Layland, S. L., Kraushaar, U., Duffy, G. P., et al. (2019) · Matrix Biology · doi:10.1016/j.matbio.2019.06.008 · Cited by 92 · see citing works
Grattoni, A., Korbutt, G., Tomei, A. A., García, A. J., Pepper, A. R., Stabler, C., Brehm, M., Papas, K., Citro, A., Shirwan, H., et al. (2025) · Nature Reviews Endocrinology · doi:10.1038/s41574-024-01029-0 · Cited by 92 · see citing works
Self-citationAugsornworawat, P., Hogrebe, N. J., Ishahak, M., Schmidt, M. D., Marquez, E., Maestas, M. M., Veronese-Paniagua, D., Gale, S. E., Miller, J. R., Velazco-Cruz, L., et al. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01150-8 · Cited by 85 · see citing works
Self-citationβ-Cell Maturation and Identity in Health and Disease
Salinno, C., Cota, P., Bastidas-Ponce, A., Tarquis-Medina, M., Lickert, H., & Bakhti, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20215417 · Cited by 84 · see citing works
Silva, I. B. B., Kimura, C. H., Colantoni, V. P., & Sogayar, M. (2022) · Stem cell research & therapeutics · doi:10.1186/s13287-022-02977-y · Cited by 68 · see citing works
Zhu, H., Wang, G., Nguyen-Ngoc, K.-V., Kim, D., Miller, M., Goss, G., Kovsky, J., Harrington, A. R., Saunders, D. C., Hopkirk, A. L., et al. (2023) · Developmental Cell · doi:10.1016/j.devcel.2023.03.011 · Cited by 85 · see citing works
Towards a better understanding of diabetes mellitus using organoid models
Beydag-Tasöz, B. S., Yennek, S., & Grapin‐Botton, A. (2023) · Nature Reviews Endocrinology · doi:10.1038/s41574-022-00797-x · Cited by 83 · see citing works
The Human Islet: Mini-organ with Mega-impact.
Walker, J. T., Saunders, D. C., Brissova, M., & Powers, A. (2021) · Endocrine reviews · doi:10.1210/endrev/bnab010 · Cited by 67 · see citing works
Deciphering early human pancreas development at the single-cell level
Ma, Z., Zhang, X., Zhong, W., Yi, H., Chen, X., Zhao, Y., Ma, Y., Song, E., & Xu, T. (2023) · Nature Communications · doi:10.1038/s41467-023-40893-8 · Cited by 76 · see citing works
Type 1 Diabetes Mellitus: A Review on Advances and Challenges in Creating Insulin Producing Devices
Oliveira, S. M. R., Rebocho, A., Ahmadpour, E., Nissapatorn, V., & Pereira, M. D. L. (2023) · Micromachines · doi:10.3390/mi14010151 · Cited by 84 · see citing works
Towards a Functional Cure for Diabetes Using Stem Cell-Derived Beta Cells: Are We There Yet?
Bourgeois, S., Sawatani, T., Mulders, A. V., Leu, N. D., Heremans, Y., Heimberg, H., Cnop, M., & Staels, W. (2021) · Cells · doi:10.3390/cells10010191 · Cited by 61 · see citing works
Fattahi, P., Rahimian, A., Slama, M. Q., Gwon, K., Gonzalez‐Suarez, A. M., Wolf, J., Baskaran, H., Duffy, C. D., Stybayeva, G., Peterson, Q. P., et al. (2021) · Scientific Reports · doi:10.1038/s41598-021-85786-2 · Cited by 69 · see citing works
ENTPD3 Marks Mature Stem Cell–Derived β-Cells Formed by Self-Aggregation In Vitro
Docherty, F. M., Riemondy, K. A., Castro-Gutiérrez, R., Dwulet, J. M., Shilleh, A. H., Hansen, M. S., Williams, S. P. M., Armitage, L. H., Santostefano, K. E., Wallet, M. A., et al. (2021) · Diabetes · doi:10.2337/db20-0873 · Cited by 69 · see citing works
Recent advances in the applications of iPSC technology
Wiegand, C., & Banerjee, I. (2019) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2019.05.011 · Cited by 67 · see citing works
Goswami, D., Domingo‐Lopez, D. A., Ward, N. A., Millman, J. R., Duffy, G. P., Dolan, E. B., & Roche, E. T. (2021) · Advanced Science · doi:10.1002/advs.202100820 · Cited by 68 · see citing works
Self-citationAdvances Toward Engineering Functionally Mature Human Pluripotent Stem Cell-Derived β Cells
Velazco-Cruz, L., Goedegebuure, M. M., & Millman, J. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00786 · Cited by 62 · see citing works
Self-citationWeng, C., Xi, J., Li, H., Cui, J., Gu, A., Lai, S., Leskov, K., Ke, L., Jin, F., & Li, Y. (2020) · Nature Metabolism · doi:10.1038/s42255-020-00314-2 · Cited by 65 · see citing works
Maturation of beta cells: lessons from in vivo and in vitro models
Barsby, T., & Otonkoski, T. (2022) · Diabetologia · doi:10.1007/s00125-022-05672-y · Cited by 66 · see citing works
Aghazadeh, Y., Sarangi, F., Poon, F., Nkennor, B., McGaugh, E. C., Nunes, S. S., & Nostro, M. C. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.03.004 · Cited by 62 · see citing works
Protecting Stem Cell Derived Pancreatic Beta-Like Cells From Diabetogenic T Cell Recognition
Castro-Gutiérrez, R., Alkanani, A. K., Mathews, C. E., Michels, A. W., & Russ, H. A. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.707881 · Cited by 60 · see citing works
Human pluripotent stem cell-derived insulin-producing cells: A regenerative medicine perspective
Migliorini, A., Nostro, M., & Sneddon, J. B. (2021) · Cell Metabolism · doi:10.1016/j.cmet.2021.03.021 · Cited by 60 · see citing works
Loss of the transcription factor MAFB limits β-cell derivation from human PSCs
Russell, R., Carnese, P., Hennings, T. G., Walker, E. M., Russ, H. A., Liu, J. S., Giacometti, S., Stein, R. W., & Hebrok, M. (2020) · Nature Communications · doi:10.1038/s41467-020-16550-9 · Cited by 61 · see citing works
Differentiating functional human islet-like aggregates from pluripotent stem cells
Barsby, T., Ibrahim, H., Lithovius, V., Montaser, H., Balboa, D., Vähäkangas, E., Chandra, V., Saarimäki‐Vire, J., & Otonkoski, T. (2022) · STAR Protocols · doi:10.1016/j.xpro.2022.101711 · Cited by 58 · see citing works
Differential Function and Maturation of Human Stem Cell-Derived Islets After Transplantation
Maxwell, K. G., Kim, M. H., Gale, S. E., & Millman, J. R. (2022) · Stem Cells Translational Medicine · doi:10.1093/stcltm/szab013 · Cited by 54 · see citing works
Self-citationPancreatic islet transplantation: current advances and challenges
Wang, Q., Huang, Y.-X., Liu, L., Zhao, X.-H., Sun, Y., Mao, X.-L., & Li, S.-W. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1391504 · Cited by 63 · see citing works
Transcriptional mechanisms of pancreatic β-cell maturation and functional adaptation
Wortham, M., & Sander, M. (2021) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2021.04.011 · Cited by 56 · see citing works
Human expandable pancreatic progenitor–derived β cells ameliorate diabetes
Ma, X., Lu, Y., Zhou, Z., Li, Q., Chen, X., Wang, W., Jin, Y., Hu, Z., Chen, G., Deng, Q., et al. (2022) · Science Advances · doi:10.1126/sciadv.abk1826 · Cited by 60 · see citing works
Islet organoid as a promising model for diabetes
Zhang, X., Ma, Z., Song, E., & Xu, T. (2021) · Protein & Cell · doi:10.1007/s13238-021-00831-0 · Cited by 52 · see citing works
Emerging diabetes therapies: Bringing back the β-cells
Basile, G., Qadir, F., Mauvais-Jarvis, F., Vetere, A., Shoba, V., Modell, A. E., Pastori, R., Russ, H., Wagner, B., & Domínguez-Bendala, J. (2022) · Molecular Metabolism · doi:10.1016/j.molmet.2022.101477 · Cited by 47 · see citing works
A Stem Cell Approach to Cure Type 1 Diabetes.Preprint
Helman, A., & Melton, D. (2020) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a035741 · Cited by 48 · see citing works
Youngblood, R., Sampson, J. P., Lebioda, K. R., & Shea, L. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.06.032 · Cited by 44 · see citing works
Augsornworawat, P., Velazco-Cruz, L., Song, J., & Millman, J. R. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.08.031 · Cited by 51 · see citing works
Self-citationAdvances in Cell Replacement Therapies for Diabetes
Hering, B. J., Rickels, M. R., Bellin, M. D., Millman, J. R., Tomei, A. A., Garcı́a, A. J., Shirwan, H., Stabler, C. L., Ma, M., Peng, Y., et al. (2025) · Diabetes · doi:10.2337/db25-0037 · Cited by 49 · see citing works
Self-citationCurrent status of stem cell therapy for type 1 diabetes: a critique and a prospective consideration
Ghoneim, M. A., Gabr, M. M., El-Halawani, S. M., & Refaie, A. F. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03636-0 · Cited by 49 · see citing works
Chmielowiec, J., Szlachcic, W. J., Yang, D., Scavuzzo, M. A., Wamble, K., Sarrion-Perdigones, A., Sabek, O., Venken, K., & Borowiak, M. (2022) · Nature Communications · doi:10.1038/s41467-022-29646-1 · Cited by 43 · see citing works
Yung, T. M., Poon, F., Liang, M., Coquenlorge, S., McGaugh, E. C., Hui, C., Wilson, M. D., Nostro, M. C., & Kim, T.-H. (2019) · Nature Communications · doi:10.1038/s41467-019-12624-5 · Cited by 44 · see citing works
Pancreas-on-a-Chip Technology for Transplantation Applications
Abadpour, S., Aizenshtadt, A., Olsen, P. A., Shoji, K., Wilson, S., Krauss, S., & Scholz, H. (2020) · Current Diabetes Reports · doi:10.1007/s11892-020-01357-1 · Cited by 36 · see citing works
Reduced replication fork speed promotes pancreatic endocrine differentiation and controls graft size
Sui, L., Xin, Y., Du, Q., Georgieva, D., Diedenhofen, G., Haataja, L., Su, Q., Zuccaro, M. V., Kim, J., Fu, J., et al. (2021) · JCI Insight · doi:10.1172/jci.insight.141553 · Cited by 45 · see citing works
Blanchi, B., Taurand, M., Colace, C., Thomaidou, S., Audeoud, C., Fantuzzi, F., Sawatani, T., Gheibi, S., Sabadell-Basallote, J., Boot, F., et al. (2023) · Molecular Metabolism · doi:10.1016/j.molmet.2023.101772 · Cited by 42 · see citing works
Identification of unique cell type responses in pancreatic islets to stress
Maestas, M. M., Ishahak, M., Augsornworawat, P., Veronese-Paniagua, D., Maxwell, K. G., Velazco-Cruz, L., Marquez, E., Sun, J., Shunkarova, M., Gale, S. E., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49724-w · Cited by 51 · see citing works
Self-citationHuman Pluripotent Stem Cells to Model Islet Defects in Diabetes
Balboa, D., Iworima, D. G., & Kieffer, T. J. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.642152 · Cited by 47 · see citing works
Fantuzzi, F., Toivonen, S., Schiavo, A. A., Chae, H., Mohammad, T., Sawatani, T., Pachera, N., Cai, Y., Vinci, C., Virgilio, E., et al. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.967765 · Cited by 42 · see citing works
Cell therapy for type 1 diabetes
Loretelli, C., Assi, E., Seelam, A., Nasr, M. B., & Fiorina, P. (2020) · Expert Opinion on Biological Therapy · doi:10.1080/14712598.2020.1748596 · Cited by 29 · see citing works
SIX2 Regulates Human β Cell Differentiation from Stem Cells and Functional Maturation In Vitro
Velazco-Cruz, L., Goedegebuure, M. M., Maxwell, K. G., Augsornworawat, P., Hogrebe, N. J., & Millman, J. R. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107687 · Cited by 44 · see citing works
Self-citationBatra, L., Shrestha, P., Zhao, H., Woodward, K. B., Togay, A., Tan, M., Grimany-Nuno, O., Malik, M. T., Coronel, M. M., Garcı́a, A. J., et al. (2020) · The Journal of Immunology · doi:10.4049/jimmunol.2000055 · Cited by 42 · see citing works
Emerging strategies for beta cell transplantation to treat diabetes
Paez‐Mayorga, J., Lukin, I., Emerich, D. F., de Vos, P., Orive, G., & Grattoni, A. (2021) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2021.11.007 · Cited by 43 · see citing works
Characterization of stem-cell-derived islets during differentiation and after implantation
Cuesta-Gomez, N., Verhoeff, K., Jasra, I. T., Pawlick, R., Dadheech, N., & Shapiro, A. M. J. (2022) · Cell Reports · doi:10.1016/j.celrep.2022.111238 · Cited by 41 · see citing works
Immune Protection of Stem Cell-Derived Islet Cell Therapy for Treating Diabetes
Tahbaz, M., & Yoshihara, E. (2021) · Frontiers in Endocrinology · doi:10.3389/fendo.2021.716625 · Cited by 42 · see citing works
Type 1 diabetes and engineering enhanced islet transplantation
Jeyagaran, A., Lu, C., Zbinden, A., Birkenfeld, A. L., Brucker, S. Y., & Layland, S. L. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114481 · Cited by 41 · see citing works
Strategies to Improve the Safety of iPSC-Derived β Cells for β Cell Replacement in Diabetes
Pellegrini, S., Zamarian, V., & Sordi, V. (2022) · Transplant International · doi:10.3389/ti.2022.10575 · Cited by 38 · see citing works
Barra, J. M., Robino, R. A., Castro-Gutiérrez, R., Proia, J., Russ, H. A., & Ferreira, L. M. R. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.114994 · Cited by 39 · see citing works
Modeling different types of diabetes using human pluripotent stem cells
Abdelalim, E. M. (2020) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03710-9 · Cited by 38 · see citing works
Mimicking nature-made beta cells: recent advances towards stem cell-derived islets
Tremmel, D. M., Mitchell, S. A., Sackett, S. D., & Odorico, J. S. (2019) · Current Opinion in Organ Transplantation · doi:10.1097/mot.0000000000000687 · Cited by 37 · see citing works
Deciphering the Complex Communication Networks That Orchestrate Pancreatic Islet Function
Weitz, J., Menegaz, D., & Caicedo, A. (2020) · Diabetes · doi:10.2337/dbi19-0033 · Cited by 37 · see citing works
Development of a scalable method to isolate subsets of stem cell-derived pancreatic islet cells
Parent, A. V., Ashe, S., Nair, G. G., Li, M.-L., Chavez, J., Liu, J. S., Zhong, Y., Streeter, P. R., & Hebrok, M. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.02.001 · Cited by 37 · see citing works
Iworima, D. G., Baker, R. K., Ellis, C. E., Sherwood, C., Zhan, L., Rezania, A., Piret, J. M., & Kieffer, T. J. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-023-03574-3 · Cited by 35 · see citing works
Basile, G., Kahraman, S., Dirice, E., Pan, H., Dreyfuss, J. M., & Kulkarni, R. N. (2021) · Genome Medicine · doi:10.1186/s13073-021-00941-8 · Cited by 33 · see citing works
Krentz, N. A. J., Shea, L. D., Huising, M. O., & Shaw, J. (2021) · The Lancet Diabetes & Endocrinology · doi:10.1016/s2213-8587(21)00170-4 · Cited by 37 · see citing works
A Century-long Journey From the Discovery of Insulin to the Implantation of Stem Cell–derived Islets
Ramzy, A., Belmonte, P. J., Braam, M. J. S., Ida, S., Wilts, E. M., Levings, M. K., Rezania, A., & Kieffer, T. J. (2022) · Endocrine Reviews · doi:10.1210/endrev/bnac021 · Cited by 36 · see citing works
Pancreatic β-Cell Development and RegenerationPreprint
Kerper, N. R., Ashe, S., & Hebrok, M. (2021) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a040741 · Cited by 38 · see citing works
SUR1-mutant iPS cell-derived islets recapitulate the pathophysiology of congenital hyperinsulinism
Lithovius, V., Saarimäki-Vire, J., Balboa, D., Ibrahim, H., Montaser, H., Barsby, T., & Otonkoski, T. (2021) · Diabetologia · doi:10.1007/s00125-020-05346-7 · Cited by 34 · see citing works
Genetic Engineering of Immune Evasive Stem Cell-Derived Islets
Sackett, S. D., Kaplan, S. J., Mitchell, S. A., Brown, M. E., Burrack, A. L., Grey, S., Huangfu, D., & Odorico, J. (2022) · Transplant International · doi:10.3389/ti.2022.10817 · Cited by 32 · see citing works
Pancreatic β-cell heterogeneity in adult human islets and stem cell-derived islets
Aldous, N., Moin, A., & Abdelalim, E. M. (2023) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-023-04815-7 · Cited by 20 · see citing works
Pancreatic β-cell failure, clinical implications, and therapeutic strategies in type 2 diabetes
Cui, D., Feng, X., Lei, S., Zhang, H., Hu, W., Yang, S.-S., Yu, X.-Q., & Su, Z.-G. (2024) · Chinese Medical Journal · doi:10.1097/cm9.0000000000003034 · Cited by 28 · see citing works
Jun, Y., Nguyen-Ngoc, K.-V., Sai, S., Bender, R. H. F., Gong, W., Kravets, V. V., Zhu, H., Hatch, C. J., Schlichting, M., Gaetani, R., et al. (2022) · Developmental Cell · doi:10.1016/j.devcel.2025.04.024 · Cited by 42 · see citing works
Kim, M. J., Lee, E. Y., You, Y., Yang, H. K., Yoon, K., & Kim, J.-W. (2020) · Stem Cell Research · doi:10.1016/j.scr.2020.101958 · Cited by 34 · see citing works
Li, H., Zhu, H., Ge, T., Wang, Z., & Zhang, C. (2021) · Stem Cell Reviews and Reports · doi:10.1007/s12015-021-10139-5 · Cited by 35 · see citing works
Andersson-Rolf, A., Groot, K., Korving, J., Begthel, H., Hanegraaf, M. A. J., VanInsberghe, M. A., Salmén, F., van den Brink, S., López‐Iglesias, C., Peters, P. J., et al. (2024) · Cell · doi:10.1016/j.cell.2024.10.044 · Cited by 34 · see citing works
Stem Cells, Self-Renewal, and Lineage Commitment in the Endocrine System
Mariniello, K., Ruiz-Babot, G., McGaugh, E. C., Nicholson, J., Gualtieri, A., Gaston-Massuet, C., Nostro, M., & Guasti, L. (2019) · Frontiers in Endocrinology · doi:10.3389/fendo.2019.00772 · Cited by 26 · see citing works
The hepatokine fetuin-A disrupts functional maturation of pancreatic beta cells
Gerst, F., Kemter, E., Lorza‐Gil, E., Kaiser, G., Fritz, A.-K., Nano, R., Piemonti, L., Gauder, M., Dahl, A., Nadalin, S., et al. (2021) · Diabetologia · doi:10.1007/s00125-021-05435-1 · Cited by 31 · see citing works
Generation of Pancreatic Progenitors from Human Pluripotent Stem Cells by Small Molecules
Jiang, Y., Chen, C., Randolph, L. N., Ye, S., Zhang, X., Bao, X., & Lian, X. L. (2020) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.07.021 · Cited by 32 · see citing works
Pignatelli, C., Campo, F., Neroni, A., Piemonti, L., & Citro, A. (2022) · Transplant International · doi:10.3389/ti.2022.10555 · Cited by 25 · see citing works
Schmidt, M. D., Ishahak, M., Augsornworawat, P., & Millman, J. R. (2024) · BMC Genomics · doi:10.1186/s12864-024-10013-x · Cited by 30 · see citing works
Self-citationEncapsulation and immune protection for type 1 diabetes cell therapy
Kioulaphides, S., & Garcı́a, A. J. (2024) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2024.115205 · Cited by 34 · see citing works
Genome editing of human pancreatic beta cell models: problems, possibilities and outlook
Balboa, D., Prasad, R. B., Groop, L., & Otonkoski, T. (2019) · Diabetologia · doi:10.1007/s00125-019-4908-z · Cited by 30 · see citing works
Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets
Kim, M., Cho, S., Hwang, D. G., Shim, I., Kim, S. C., Jang, J., & Jang, J.-A. (2025) · Nature Communications · doi:10.1038/s41467-025-56665-5 · Cited by 31 · see citing works
- Authors
- Velazco-Cruz, L., Song, J., Maxwell, K. G., Goedegebuure, M. M., Augsornworawat, P., Hogrebe, N. J., & Millman, J. R.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Millman, Jeffrey R.
- ORCID
- Venue
- Stem Cell Reports
- Publisher
- Elsevier BV
- Published
- 2019-02
- Volume
- 12
- Issue
- 2
- Pages
- 351-365
- ISSN
- 2213-6711 (ISSN-L)
- PMC
- PMC6372986
- OpenAlex
- W2910245538
- Cited by
- 399
- References
- 57
- Citations by year
- 2026: 45 · 2025: 49 · 2024: 54 · 2023: 56 · 2022: 61 · 2021: 72 · 2020: 42 · 2019: 20
- FWCI
- 46.82
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cell Reports (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Digital Commons@Becker (Washington University School of Medicine) (submittedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- NIH (5R01DK114233, 5P30DK020579, 2R25GM103757, 5T32DK108742, 5T32DK007120)
- JDRF (5-CDA-2017-391-A-N)
- Washington University Center of Regenerative Medicine
- Washington University School of Medicine
- Hope Center Viral Vectors Core
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Diabetes and associated disorders
- Keywords
- stem cell-derived beta cells, human pluripotent stem cells, glucose-stimulated insulin secretion, TGF-beta signaling, beta cell differentiation, serum-free medium, islet transplantation, glucose tolerance, diabetes cell therapy
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, Glucose, Humans, Insulin, Pancreas, Signal Transduction, Transforming Growth Factor beta, Pluripotent Stem Cells, Insulin-Secreting Cells, Mice, Embryonic Stem Cells, Cell- and Tissue-Based Therapy
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