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Cited by 10Fungal and yeast genetics researchRNA Research and SplicingAntifungal resistance and susceptibility

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A posttranscriptional pathway regulates cell wall mRNA expression in budding yeast

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Bresson, Stefan, Shchepachev, Vadim, Tollervey, David·2023·Cell Reports
DOI 10.1016/j.celrep.2023.112184

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Abstract

The fungal cell wall provides protection and structure and is an important target for antifungal compounds. A mitogen-activated protein (MAP) kinase cascade termed the cell wall integrity (CWI) pathway regulates transcriptional responses to cell wall damage. Here, we describe a posttranscriptional pathway that plays an important complementary role. We report that the RNA-binding proteins (RBPs) Mrn1 and Nab6 specifically target the 3' UTRs of a largely overlapping set of cell wall-related mRNAs. These mRNAs are downregulated in the absence of Nab6, indicating a function in target mRNA stabilization. Nab6 acts in parallel to CWI signaling to maintain appropriate expression of cell wall genes during stress. Cells lacking both pathways are hypersensitive to antifungal compounds targeting the cell wall. Deletion of MRN1 partially alleviates growth defects associated with Δnab6, and Mrn1 has an opposing function in mRNA destabilization. Our results uncover a posttranscriptional pathway that mediates cellular resistance to antifungal compounds.

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Metadata

Authors
Bresson, S., Shchepachev, V., & Tollervey, D.
Affiliations
Wellcome Centre for Cell Biology (GB)
Corresponding author
  • Bresson, Stefan
  • Tollervey, David
ORCID
Venue
Cell Reports
Publisher
Elsevier BV
Published
2023-03
Volume
42
Issue
3
Pages
112184
ISSN
2211-1247 (ISSN-L)
DOI
10.1016/j.celrep.2023.112184
OpenAlex
W4322603888
Cited by
10
References
8
Citations by year
2026: 3 · 2025: 3 · 2024: 3 · 2023: 1
FWCI
0.89
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Reports (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by)
Funders
Wellcome Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
cell wall integrity pathway, post-transcriptional regulation, RNA-binding proteins, 3' untranslated region, mRNA stability, mRNA destabilization, antifungal resistance, cell wall stress, Saccharomyces cerevisiae, MAPK signaling
MeSH terms
Antifungal Agents, Cell Wall, Saccharomycetales, RNA, Messenger, Saccharomyces cerevisiae, Gene Expression Regulation, Fungal, Saccharomyces cerevisiae Proteins

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