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Gut-Liver Physiomimetics Reveal Paradoxical Modulation of IBD-Related Inflammation by Short-Chain Fatty Acids

Trapecar, Martin, Communal, Catherine, Velazquez, Jason, Maass, Christian Alexander, Huang, Yu-Ja, Schneider, Kirsten, Wright, Charles W., Butty, Vincent +4 more·2020·Cell Systems
DOI 10.1016/j.cels.2020.02.008PMC PMC8143761

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  • In cell-culture insert systems, cellular uptake and transport across the cell barrier can be studied with the porous membrane.

    “For the cell-culture insert system, cellular uptake and transport across the cell barrier (e.g., endothelial cells and intestinal epithelial cells) can be studied with the porous membrane;”

    From Gut-liver-axis microphysiological system for studying cellular fluidic shear stress and inter-tissue interaction (2022) · cites this paper as [7]

    The STAR Methods describe intestinal epithelial monolayers grown on porous Transwell inserts, and the Results show that the system was used to study short-chain fatty acid passage across the gut barrier and its consumption by the gut model. The cited paper does not establish the sentence’s endothelial-cell example; its barrier experiments concern intestinal epithelium. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Reported GLA-MPSs showed better predictive capabilities of organ-to-organ interaction compared to normal static cell-culture models.

    “They showed better predictive capabilities of organ to organ interaction, compared to normal static cell-culture models.”

    From Gut-liver-axis microphysiological system for studying cellular fluidic shear stress and inter-tissue interaction (2022) · cites this paper as [7]

    The Results section ('Interaction between SCFA, gut and liver MPSs') reports that connecting the gut and liver MPSs changed their phenotypes and gene expression relative to studying them in isolation. This supports the claim that the system reveals organ-to-organ interactions, but the paper does not establish better predictive capability than static cell-culture models; its isolation comparison is not identified as a static-culture comparison. Page numbers are unavailable in our copy. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Current GLA-MPSs are based on two approaches: the cell-culture insert in microtiter plates and microfluidic devices.

    “The current GLA-MPSs are based on two approaches: the cell-culture insert in the microtiter plates 7,11 and microfluidic devices.12,13”

    From Gut-liver-axis microphysiological system for studying cellular fluidic shear stress and inter-tissue interaction (2022) · cites this paper as [7]

  • A number of MPSs as an in vitro GLA model (GLA-MPS) have been reported, such as GLA for metabolism and inflammation axes.

    “Thus far, a number of MPSs as an in vitro GLA model (GLA-MPS) have been reported, such as GLA for metabolism and inflammation axess.3,7- 11”

    From Gut-liver-axis microphysiological system for studying cellular fluidic shear stress and inter-tissue interaction (2022) · cites this paper as [7]

  • In other GLA-MPSs, the exchange ratio is approximately 0.25% to 1% in 1 min.

    “In other GLA-MPSs, 3,7 the exchange ratio is approximately 0.25% to 1% in 1 min.”

    From Gut-liver-axis microphysiological system for studying cellular fluidic shear stress and inter-tissue interaction (2022) · cites this paper as [7]

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Cited by

Sorted by citations

Authors
Trapecar, M., Communal, C., Velazquez, J., Maass, C. A., Huang, Y.-J., Schneider, K., Wright, C. W., Butty, V., Eng, G., Yilmaz, O., Trumper, D., & Griffith, L. G.
Venue
Cell Systems
Publisher
Elsevier BV
Published
2020-03
Volume
10
Issue
3
Pages
223-239.e9
ISSN
2405-4712
DOI
10.1016/j.cels.2020.02.008
PMC
PMC8143761
Cited by
204
References
9
Language
en
Licence
Funders
  • National Institute of Environmental Health Sciences (P30-ES002109)
  • Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (R01 CA211184, CA034992, P30-CA14051, P30DK034854)
  • National Institute of Biomedical Imaging and Bioengineering (R01EB021908)
  • National Institutes of Health (P30DK034854, CA034992)
  • Pew Charitable Trusts
  • Defense Advanced Research Projects Agency (W911NF-12-2-0039)

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