Kinesin-3 Mediates Axonal Sorting and Directional Transport of Alphaherpesvirus Particles in Neurons
Open accessAbstract
During infection of the nervous system, alphaherpesviruses-including pseudorabies virus (PRV)-use retrograde axonal transport to travel toward the neuronal cell body and anterograde transport to traffic back to the cell periphery upon reactivation from latency. The PRV protein Us9 plays an essential but unknown role in anterograde viral spread. To determine Us9 function, we identified viral and host proteins that interact with Us9 and explored the role of KIF1A, a microtubule-dependent kinesin-3 motor involved in axonal sorting and transport. Viral particles are cotransported with KIF1A in axons of primary rat superior cervical ganglion neurons, and overexpression or disruption of KIF1A function, respectively, increases and reduces anterograde capsid transport. Us9 and KIF1A interact early during infection with the aid of additional viral protein(s) but exhibit diminished binding at later stages, when capsids typically stall in axons. Thus, alphaherpesviruses repurpose the axonal transport and sorting pathway to spread within their hosts.
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In PRV-infected neurons, Us9 interacts with KIF1A, and KIF1A is required for viral capsid transport in axons and for infection spread.
“the Enquist research group found that the viral protein Us9 interacts with the cellular kinesin KIF1A.”
From Proteomic approaches to uncovering virus–host protein interactions during the progression of viral infection (2016) · cites this paper as [42]
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- Authors
- Kramer, T., Greco, T. M., Taylor, M. P., Ambrosini, A. E., Cristea, I. M., & Enquist, L. W.
- Affiliations
- Princeton University (US)
- Corresponding author
- Enquist, Lynn W.
- Venue
- Cell Host & Microbe
- Publisher
- Elsevier BV
- Published
- 2012-12
- Volume
- 12
- Issue
- 6
- Pages
- 806-814
- ISSN
- 1931-3128 (ISSN-L)
- PMC
- PMC3527838
- OpenAlex
- W2048449303
- Cited by
- 102
- References
- 27
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 5 · 2022: 8 · 2021: 8 · 2020: 13 · 2019: 9 · 2018: 5 · 2017: 6 · 2016: 6 · 2015: 12 · 2014: 7 · 2013: 10
- FWCI
- 6.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell Host & Microbe (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute on Drug Abuse (DP1DA026192)
- National Cancer Institute (P01CA087661)
- National Institute of Neurological Disorders and Stroke (R01NS060699, R01NS033506, R37 NS33506, R37NS033506)
- National Center for Research Resources (P40RR018604, P40 RR18604)
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Keywords
- alphaherpesvirus, pseudorabies virus, KIF1A, kinesin-3, axonal transport, retrograde transport, viral protein-protein interaction, superior cervical ganglion neurons, viral spread, latency reactivation
- MeSH terms
- Animals, Cells, Cultured, Lipoproteins, Neurons, Phosphoproteins, Herpesvirus 1, Suid, Viral Proteins, Kinesins, Protein Interaction Mapping, Intracellular Signaling Peptides and Proteins, Rats, Host-Pathogen Interactions
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