Cited by 270Influenza Virus Research StudiesMonoclonal and Polyclonal Antibodies Researchvaccines and immunoinformatics approaches

Both Neutralizing and Non-Neutralizing Human H7N9 Influenza Vaccine-Induced Monoclonal Antibodies Confer Protection

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Henry Dunand, Carole J., Leon, Paul E., Huang, Min, Choi, Angela, Chromikova, Veronika, Ho, Irvin Y., Tan, Gene S., Cruz, John +11 more·2016·Cell Host & Microbe

Abstract

Pathogenic H7N9 avian influenza viruses continue to represent a public health concern, and several candidate vaccines are currently being developed. It is vital to assess if protective antibodies are induced following vaccination and to characterize the diversity of epitopes targeted. Here we characterized the binding and functional properties of twelve H7-reactive human antibodies induced by a candidate A/Anhui/1/2013 (H7N9) vaccine. Both neutralizing and non-neutralizing antibodies protected mice in vivo during passive transfer challenge experiments. Mapping the H7 hemagglutinin antigenic sites by generating escape mutant variants against the neutralizing antibodies identified unique epitopes on the head and stalk domains. Further, the broadly cross-reactive non-neutralizing antibodies generated in this study were protective through Fc-mediated effector cell recruitment. These findings reveal important properties of vaccine-induced antibodies and provide a better understanding of the human monoclonal antibody response to influenza in the context of vaccines.

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Authors
Henry Dunand, C. J., Leon, P. E., Huang, M., Choi, A., Chromikova, V., Ho, I. Y., Tan, G. S., Cruz, J., Hirsh, A., Zheng, N.-Y., Mullarkey, C. E., Ennis, F. A., Terajima, M., Treanor, J. J., Topham, D. J., Subbarao, K., Palese, P., Krammer, F., & Wilson, P. C.
Affiliations
  • Icahn School of Medicine at Mount Sinai (US)
  • University of Chicago (US)
  • University of Massachusetts Chan Medical School (US)
  • University of Rochester Medicine (US)
  • National Institutes of Health (US)
Corresponding author
  • Krammer, Florian
  • Wilson, Patrick C.
Venue
Cell Host & Microbe
Publisher
Elsevier BV
Published
2016-06
Volume
19
Issue
6
Pages
800-813
ISSN
1931-3128 (ISSN-L)
OpenAlex
W2417981936
Cited by
268
References
53
Citations by year
2026: 10 · 2025: 12 · 2024: 18 · 2023: 19 · 2022: 23 · 2021: 32 · 2020: 32 · 2019: 30 · 2018: 37 · 2017: 42 · 2016: 13
FWCI
38.38
Type
journal article
Language
en
Open access
Locations
  • Cell Host & Microbe (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • World Health Organization
  • National Institute of Allergy and Infectious Diseases (HHSN272201400008C), U19AI109946, P01AI097092, HHSN272200900026C, HHSN272201400005C, U19AI082724, U19AI057266, HHSN 272201400006C)
Topics
  • Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Keywords
H7N9 avian influenza, influenza vaccination, monoclonal antibodies, neutralizing antibodies, non-neutralizing antibodies, hemagglutinin epitope, hemagglutinin stalk domain, cross-reactive antibodies, vaccine-induced antibody response
MeSH terms
Animals, Antibodies, Monoclonal, Antibodies, Viral, Antigens, Viral, Disease Models, Animal, Dogs, Female, Humans, Influenza, Human, Influenza Vaccines, Mice, Inbred BALB C, Orthomyxoviridae Infections, Hemagglutinin Glycoproteins, Influenza Virus, Mice, Antibodies, Neutralizing, HEK293 Cells, Madin Darby Canine Kidney Cells, Influenza A Virus, H7N9 Subtype

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