Hyphenated technique for the extraction and determination of isoflavones in algae: Ultrasound-assisted supercritical fluid extraction followed by fast chromatography with tandem mass spectrometry
Abstract
New hyphenated technique for the extraction and determination of isoflavones in sea and freshwater algae and cyanobacteria was developed. The method consists of sonication sample pretreatment, extraction by supercritical CO(2) modified by 3% (v/v) of MeOH/H(2)O mixture (9:1, v/v) at 35 MPa and 40°C for 60 min, fast chromatography analysis by the means of Agilent 1200 Series Rapid Resolution and MS/MS determination. Agilent 1200 Series RRLC was used with Zorbax SB-CN chromatographic column (100 mm × 2.1mm, particle size 3.5 μm), 3μl injection volume, mobile phase consisting of 0.2% (v/v) acetic acid in water (solvent A) and acetonitrile (solvent B) and used with linear gradient (30% B at 0 min, from 0 min to 3 min up to 50% B, from 3 to 6 min up to 80% B and from 6 to 10 min down to 30% B). The flow-rate was 0.4 mL/min, column oven temperature 35°C. MS detector Agilent Technologies 6460 Triple quadrupole LC/MS with Agilent Jet Stream was used in a negative ESI mode under following conditions: gas temperature 350°C, gas flow 13 L/min, nebulizer gas pressure 50 psi, sheath gas temperature 400°C, sheath gas flow 12L/min, capillary voltage was 4 kV. Samples were analysed in the multiple reaction monitoring (MRM) mode. Eight isoflavone compounds were found for the first time in seven real samples of sea algae and in three control samples of freshwater algae and cyanobacteria. Usual optimisation study of extraction parameters was performed. Pressure and temperature optima for algae matrix are different from those obtained sooner for other matrices for most of the analytes, but the results of modifier optimisation study are in good accordance with those obtained sooner for spiked samples and red clover matrix. It seems that matrix has very small or no effect on the modifier selection. Two different approaches of sonication pretreatment were tested: sonication bath and the thorn instrument. In longer extraction time experiments, thorn sonication was more efficient and recovery of following supercritical fluid extraction was higher.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Some authors used SC-CO2 for extraction of lipids, volatile compounds, isoflavones, plant growth hormones, and micro- and macroelements from various types of seaweeds.
“However, some authors used SC-CO2 for the extraction of lipids [41], volatile compounds [43], isoflavones [44], plant growth hormones, and micro- and macroelements from various types of seaweeds [45].”
From Overview on the Application of Modern Methods for the Extraction of Bioactive Compounds from Marine Macroalgae (2018) · cites this paper as [44]
Cites
Setchell, K. D. R., Brown, N. M., Desai, P. B., Zimmer-Nechemias, L., Wolfe, B., Brashear, W. T., Kirschner, A. S., Cassidy, A., & Heubi, J. E. (2001) · Journal of Nutrition · doi:10.1093/jn/131.4.1362s · Cited by 974 · see citing works
Soy Isoflavone Aglycones Are Absorbed Faster and in Higher Amounts than Their Glucosides in Humans
Izumi, T., Osawa, S., Obata, A., Tobe, K., Saito, M., Kataoka, S., Kikuchi, M., Piskuła, M. K., & Kubota, Y. (2000) · Journal of Nutrition · doi:10.1093/jn/130.7.1695 · Cited by 959 · see citing works
Ultrasound-assisted extraction of soy isoflavones
Rostagno, M. A., Palma, M., & Barroso, C. G. (2003) · Journal of Chromatography A · doi:10.1016/s0021-9673(03)01184-1 · Cited by 563 · see citing works
Innovative natural functional ingredients from microalgae
Plaza, M., Herrero, M., Cifuentes, A., & Ibáñez, E. (2009) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf901070g · Cited by 509 · see citing works
Modification of supercritical fluid phase behavior using polar cosolvents
Dobbs, J. M., Wong, J. M., Lahiere, R. J., & Johnston, K. P. (1987) · Industrial & Engineering Chemistry Research · doi:10.1021/ie00061a011 · Cited by 317 · see citing works
Supercritical fluids in separation science – the dreams, the reality and the future
Smith, R. M. (1999) · Journal of Chromatography A · doi:10.1016/s0021-9673(99)00617-2 · Cited by 233 · see citing works
Onofrejová, L., Vašíčková, J., Klejdus, B., Stratil, P., Mišurcová, L., Kráčmar, S., Kopecký, J., & Vacek, J. (2009) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2009.03.027 · Cited by 229 · see citing works
Sample preparation for the analysis of isoflavones from soybeans and soy foods
Rostagno, M. A., Villares, A., Guillamón, E., Garcia-Lafuente, A. M., & Martínez, J. A. (2008) · Journal of Chromatography A · doi:10.1016/j.chroma.2008.11.035 · Cited by 210 · see citing works
Klejdus, B., Kopecký, J., Benešová, L., & Vacek, J. (2008) · Journal of Chromatography A · doi:10.1016/j.chroma.2008.11.096 · Cited by 143 · see citing works
Fluid Extraction of Hops, Spices, and Tobacco with Supercritical Gases
Hubert, P., & Vitzthum, O. (1978) · Angewandte Chemie International Edition in English · doi:10.1002/anie.197807101 · Cited by 139 · see citing works
Fahmy, T. M., Paulaitis, M. E., Johnson, D. M., & McNally, M. E. P. (1993) · Analytical Chemistry · doi:10.1021/ac00058a026 · Cited by 138 · see citing works
Supercritical fluid extraction of isoflavones from soybean flour
Rostagno, M. A., Araújo, J. M. A., & Sandi, D. (2002) · Food Chemistry · doi:10.1016/s0308-8146(02)00106-1 · Cited by 126 · see citing works
Current trends in isolation, separation, determination and identification of isoflavones: A review
Vacek, J., Klejdus, B., Lojková, L., & Kubáň, V. (2008) · Journal of Separation Science · doi:10.1002/jssc.200700569 · Cited by 121 · see citing works
Ultrasound-assisted extraction of isoflavones from soy beverages blended with fruit juices
Rostagno, M. A., Palma, M., & Barroso, C. G. (2007) · Analytica Chimica Acta · doi:10.1016/j.aca.2007.07.006 · Cited by 104 · see citing works
Kang, J., Hick, L. A., & Price, W. E. (2007) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.2897 · Cited by 103 · see citing works
Supercritical Fluid Extraction and its Use in Chromatographic Sample Preparation
Westwood, S. A. (1993) · Springer eBooks · doi:10.1007/978-94-011-2164-4 · Cited by 91 · see citing works
Combinatory and hyphenated sample preparation for the determination of bioactive compounds in foods
Rostagno, M. A., D’Arrigo, M., Martínez, J. A., & Martínez, J. A. (2010) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2010.02.015 · Cited by 75 · see citing works
Klejdus, B., Mikelová, R., Petrlová, J., Potěšil, D., Adam, V., Stiborová, M., Hodek, P., Vacek, J., Kizek, R., & Kubáň, V. (2005) · Journal of Chromatography A · doi:10.1016/j.chroma.2005.05.070 · Cited by 71 · see citing works
Klejdus, B., Lojková, L., Lapčı́k, O., Koblovská, R., Moravcová, J., & Kubáň, V. (2005) · Journal of Separation Science · doi:10.1002/jssc.200500102 · Cited by 54 · see citing works
Modern supercritical fluid chromatography
Smith, R. M., Nelis, H. J. C. F., & Leenheer, A. P. D. (1990) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)84255-2 · Cited by 42 · see citing works
Modern Supercritical Fluid Chromatography
Miller, L. M., Pinkston, J. D., & Taylor, L. T. (2019) · Wiley eBooks · doi:10.1002/9781119626022 · Cited by 36 · see citing works
Supercritical Fluid Extraction and its use in Chromatographic Sample Preparation
Dean, J. (1993) · Analytica Chimica Acta · doi:10.1016/0003-2670(93)80032-g · Cited by 26 · see citing works
Klejdus, B., Lojková, L., Kula, E., Buchta, I., Hrdlička, P., & Kubáň, V. (2008) · Journal of Separation Science · doi:10.1002/jssc.200700560 · Cited by 15 · see citing works
Klejdus, B., Lojková, L., Kosina, P., Jitka Ulrichová, A., Šimánek, V., & Kubáň, V. (2007) · Talanta · doi:10.1016/j.talanta.2007.01.055 · Cited by 14 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Microalgae for High-Value Products Towards Human Health and Nutrition
Barkia, I., Saari, N., & Manning, S. R. (2019) · Marine Drugs · doi:10.3390/md17050304 · Cited by 719 · see citing works
“Phenolic compounds are secondary metabolites commonly found in all terrestrial plants as well as microalgae and cyanobacteria [176].”
BackgroundGil-Chávez, J., Villa-Rodríguez, J. A., Ayala-Zavala, J. F., Heredia, J. B., Sepulveda, D. R., Yahia, E. M., & González-Aguilar, G. A. (2013) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12005 · Cited by 658 · see citing works
Antioxidant potential of microalgae in relation to their phenolic and carotenoid content
Goiris, K., Muylaert, K., Fraeye, I., Foubert, I., Brabanter, J. D., & Cooman, L. D. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-012-9804-6 · Cited by 590 · see citing works
Application of Novel Extraction Technologies for Bioactives from Marine Algae
Kadam, S. U., Tiwari, B. K., & O’Donnell, C. P. (2013) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf400819p · Cited by 547 · see citing works
Carreira-Casais, A., Otero, P., Garcia-Perez, P., Garcia-Oliveira, P., Pereira, A. G., Carpena, M., Soria-Lopez, A., Simal-Gandara, J., & Prieto, M. A. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph18179153 · Cited by 395 · see citing works
“Parallelly, the same research group extracted isoflavones from seven algal species (S. muticum, S. vulgare, Hypnea spinella, Porphyra sp., U. pinnatifida, C. crispus and Halopytis incurvus) by ultrasound-assisted SCFE [139].”
BackgroundInfluentialUltrasound-assisted extraction for food and environmental samples
Picó, Y. (2012) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2012.12.005 · Cited by 380 · see citing works
Phytochemical and Potential Properties of Seaweeds and Their Recent Applications: A Review
EL-BELTAGI, H. S., Mohamed, A. A., Mohamed, H. I., Ramadan, K. M. A., Barqawi, A. A., & Mansour, A. T. (2022) · Marine Drugs · doi:10.3390/md20060342 · Cited by 383 · see citing works
Ibáñez, E., Herrero, M., Mendiola, J. A., & Castro‐Puyana, M. (2011) · Springer eBooks · doi:10.1007/978-1-4614-1247-2_2 · Cited by 319 · see citing works
“…of isofl avones from sea macroalgae ( Sargassum muticum, Sargassum vulgare, Hypnea spinella, Porphyra sp. , Undaria pinnatifi da, Chondrus crispus, and Halopytis incurvus ), freshwater algae ( Spongiochloris spongiosa ), and cyanobacteria (Scenedesmus and Nostoc 17; Klejdus et al. 2010 ) .”
BackgroundMethodAlgal extracts: Technology and advances
Michalak, I., & Chojnacka, K. (2014) · Engineering in Life Sciences · doi:10.1002/elsc.201400139 · Cited by 289 · see citing works
“SFE Efficient, economical [6] Fast extraction rate (allow to avoid degradation of labile compounds) and high yield [5, 6, 16, 26] Ecofriendly technology with minimal or no use of organic solvents [5, 6, 16, 26, 45] The very low critical temperature of CO2 allows its use for thermolabile [16, 45] and easily oxidized co…”
BackgroundMethodInfluentialCikoš, A.-M., Jokić, S., Šubarić, D., & Jerković, I. (2018) · Marine Drugs · doi:10.3390/md16100348 · Cited by 225 · see citing works
“, Undaria pinnatifida, Chondrus crispus, Halopytis incurvus 10–40 35–75 10–60 Isoflavones [44]”
BackgroundAntioxidant Activity of Marine Algal Polyphenolic Compounds: A Mechanistic Approach
Fernando, I. P. S., Kim, M., Son, K.-T., Jeong, Y., & Jeon, Y. (2016) · Journal of Medicinal Food · doi:10.1089/jmf.2016.3706 · Cited by 215 · see citing works
The Evolution of Flavonoid Biosynthesis: A Bryophyte Perspective
Davies, K. M., Jibran, R., Zhou, Y., Albert, N. W., Brummell, D. A., Jordan, B. R., Bowman, J. L., & Schwinn, K. E. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.00007 · Cited by 212 · see citing works
“…there are some reports detailing specific flavonoid structures in algal preparations, including compounds such as chalcones, flavones, flavonols, isoflavonoids, and proanthocyanidins (Klejdus et al., 2010; Goiris et al., 2014; El Shoubaky et al., 2016; Agregán et al., 2017; Ben Saad et al., 2017).”
BackgroundDetection of flavonoids in microalgae from different evolutionary lineages
Goiris, K., Muylaert, K., Voorspoels, S., Noten, B., Paepe, D. D., Baart, G. J. E., & Cooman, L. D. (2014) · Journal of Phycology · doi:10.1111/jpy.12180 · Cited by 199 · see citing works
Santos, S. A. O., Félix, R., Pais, A. C. S., Rocha, S. M., & Silvestre, A. J. D. (2019) · Biomolecules · doi:10.3390/biom9120847 · Cited by 160 · see citing works
“Some authors already reported the presence of phlorotannins in SC-CO2 SFE extracts, however a more detailed characterization of the lipophilic components has pointed out the high abundance of fatty acids in the extracts obtained (which might be related with the absence of a modifier) [42].”
BackgroundMethodInfluentialBiological properties and potential of compounds extracted from red seaweeds
Carpena, M., García-Pérez, P., Garcia‐Oliveira, P., Chamorro, F., Otero, P., Lourenço‐Lopes, C., Cao, H. L., Simal‐Gándara, J., & Prieto, M. A. (2022) · Phytochemistry Reviews · doi:10.1007/s11101-022-09826-z · Cited by 162 · see citing works
“Concerning red algae, SFE has been employed to extract specially lipophilic sub-stances, such as glycolipids, phospholipids, and x -3 fatty acids (Herrero et al. 2006; Klejdus et al. 2010; Michalak and Chojnacka 2014; Terme et al. 2018).”
Mondo, A. D., Smerilli, A., Ambrosino, L., Albini, A., Noonan, D. M., Sansone, C., & Brunet, C. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1874284 · Cited by 144 · see citing works
Supercritical Fluid Extraction Using CO2: Main Applications and Future Perspectives
Machado, B. A. S., Pereira, C. G., Nunes, S. B., Padilha, F. F., & Umsza‐Guez, M. A. (2013) · Separation Science and Technology · doi:10.1080/01496395.2013.811422 · Cited by 134 · see citing works
“At present, the SFE has been applied for obtaining compounds in seaweed and micro-seaweed (14,167,168).”
MethodTzima, S., Georgiopoulou, I., Louli, V., & Magoulas, K. (2023) · Molecules · doi:10.3390/molecules28031410 · Cited by 135 · see citing works
Advances in pre-treatment techniques and green extraction technologies for bioactives from seaweeds
Ummat, V., Sivagnanam, S. P., Rajauria, G., O'Donnell, C., & Tiwari, B. K. (2021) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2021.01.018 · Cited by 126 · see citing works
Microalgae as a Source of Valuable Phenolic Compounds and Carotenoids
Cichoński, J., & Chrzanowski, G. (2022) · Molecules · doi:10.3390/molecules27248852 · Cited by 129 · see citing works
“Furthermore, phenolic acids, such as gallic, protocatechuic, caffeic, chlorogenic, vanillic, p-hydroxybenzoic, and salicylic acids, were found in different species of marine algae [17,18].”
BackgroundPlant Biostimulants to Enhance Abiotic Stress Resilience in Crops
Sario, L. D., Boeri, P. A., Matus, J. T., & Pizzio, G. A. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26031129 · Cited by 147 · see citing works
“On the other hand, among the secondary metabolites implicated in stress responses are polyphenolic compounds, including florotanins, bromophenols, flavonoids, phenolic terpenoids, mycosporine-like amino acids, and halogenated compounds [147,148].”
Lomartire, S., Cotas, J., Pacheco, D., Marques, J. C., Pereira, L., & Gonçalves, A. M. M. (2021) · Marine Drugs · doi:10.3390/md19050245 · Cited by 118 · see citing works
“(Rhodophyta) and Sargassum muticum and Sargassum vulgare (Phaeophyceae) can synthesize isoflavones, likewise daidzein or genistein [57].”
BackgroundInfluentialCheng, X., Wan, J., Li, P., & Qi, L. (2011) · Journal of Chromatography A · doi:10.1016/j.chroma.2011.06.091 · Cited by 112 · see citing works
Belghit, I., Rasinger, J. D., Heesch, S., Biancarosa, I., Liland, N. S., Torstensen, B. E., Waagbø, R., Lock, E., & Bruckner, C. G. (2017) · Algal Research · doi:10.1016/j.algal.2017.08.001 · Cited by 112 · see citing works
Microalgae for the prevention of cardiovascular disease and stroke
Raposo, M. F. D. J., & Morais, A. M. M. B. (2014) · Life Sciences · doi:10.1016/j.lfs.2014.09.018 · Cited by 109 · see citing works
Paterson, S., Gómez‐Cortés, P., de la Fuente, M. Á., & Hernández‐Ledesma, B. (2023) · Nutrients · doi:10.3390/nu15020477 · Cited by 103 · see citing works
“The antimicrobial activity of different microalgae extracts has been mainly associated to fatty acids [115], phenolic compounds [116], and pigments [117].”
BackgroundExtraction of biomolecules from seaweeds
Kadam, S. U., Álvarez, C., Tiwari, B. K., & O’Donnell, C. P. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-418697-2.00009-x · Cited by 98 · see citing works
Recent advanced applications of nanomaterials in microalgae biorefinery
Nguyen, M. K., Moon, J., Bui, V. K. H., Oh, Y., & Lee, Y. (2019) · Algal Research · doi:10.1016/j.algal.2019.101522 · Cited by 95 · see citing works
Biorefinery of marine macroalgae into high-tech bioproducts: a review
Filote, C., Santos, S. C. R., Popa, V., Botelho, C. M. S., & Volf, I. (2020) · Environmental Chemistry Letters · doi:10.1007/s10311-020-01124-4 · Cited by 97 · see citing works
Mass Spectrometry-Based Metabolomics to Elucidate Functions in Marine Organisms and Ecosystems
Goulitquer, S., Potin, P., & Tonon, T. (2012) · Marine Drugs · doi:10.3390/md10040849 · Cited by 95 · see citing works
Antioxidant and Photoprotection Networking in the Coastal Diatom Skeletonema marinoi
Smerilli, A., Balzano, S., Maselli, M., Blasio, M., Orefice, I., Galasso, C., Sansone, C., & Brunet, C. (2019) · Antioxidants · doi:10.3390/antiox8060154 · Cited by 90 · see citing works
“In contrast with higher plants, their distribution and functions in microalgae are not fully clear [14,15].”
BackgroundMichel, T., Halabalaki, M., & Skaltsounis, A. (2013) · Planta Medica · doi:10.1055/s-0032-1328300 · Cited by 89 · see citing works
Gunathilake, T., Akanbi, T. O., Suleria, H. A. R., Nalder, T. D., Francis, D. S., & Barrow, C. J. (2022) · Marine Drugs · doi:10.3390/md20070445 · Cited by 87 · see citing works
“[54] identified eight isoflavones (daidzin, daidzein, genistein, formononetin, sissotrin, biochanin A and ononin) in seven red and brown seaweeds, with the highest concentrations present in Chondrus crispus (red seaweed 86–229 ng/g) followed by Halopytis incurvus (red seaweed 7–50 ng/g) and Sargassum muticum (brown se…”
BackgroundAnalysis of Isoflavones in Foods
Bustamante-Rangel, M., Delgado‐Zamarreño, M. M., Pérez‐Martín, L., Rodríguez‐Gonzalo, E., & Domínguez‐Álvarez, J. (2018) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12325 · Cited by 80 · see citing works
Fernando, I. P. S., Lee, W., & Ahn, G. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2021.1922351 · Cited by 80 · see citing works
“The microalgae, H. pluvialis, is reported to contain the aglycone naringenin (C151) [75].”
BackgroundMethodInfluentialBello, A. S., Saadaoui, I., & Ben‐Hamadou, R. (2021) · Agronomy · doi:10.3390/agronomy11081610 · Cited by 80 · see citing works
Antibacterial activity of freshwater microalgae: A review
Pradhan, J., Sachidananda, D., & Basanta, K. D. (2014) · African Journal of Pharmacy and Pharmacology · doi:10.5897/ajpp2013.0002 · Cited by 77 · see citing works
Antimicrobial activity of compounds isolated from algae
Abu‐Ghannam, N., & Rajauria, G. (2013) · Elsevier eBooks · doi:10.1533/9780857098689.2.287 · Cited by 75 · see citing works
Antioxidant content and activity of the seaweed Saccharina latissima: a seasonal perspective
Marinho, G. S., Sørensen, A. M., Safafar, H., Pedersen, A. H., & Holdt, S. L. (2018) · Journal of Applied Phycology · doi:10.1007/s10811-018-1650-8 · Cited by 75 · see citing works
“Nevertheless, structural identification of flavonoids in red and brown seaweeds, and microalgae, has recently been reported, contradicting the general assumption that these compounds could be exclusively present in terrestrial plants (Klejdus et al. 2010; Rodríguez-Bernaldo de Quirós et al. 2010; Goiris et al. 2014).”
BackgroundMethodSeidi, S., & Yamini, Y. (2012) · Open Chemistry · doi:10.2478/s11532-011-0160-1 · Cited by 74 · see citing works
Applications of Antioxidant Secondary Metabolites of Sargassum spp.
Catarino, M. D., Silva‐Reis, R., Chouh, A., Silva, S., Braga, S. S., Silva, A. M. S., & Cardoso, S. M. (2023) · Marine Drugs · doi:10.3390/md21030172 · Cited by 68 · see citing works
Yang, Y.-C., Wei, M.-C., & Hong, S.-J. (2014) · Journal of Chromatography A · doi:10.1016/j.chroma.2013.10.098 · Cited by 66 · see citing works
Marie, B., Huet, H., Marie, A., Djédiat, C., Puiseux‐Dao, S., Catherine, A., Trinchet, I., & Edery, M. (2012) · Aquatic Toxicology · doi:10.1016/j.aquatox.2012.02.008 · Cited by 66 · see citing works
Biomass of freshwater Cladophora as a raw material for agriculture and the cosmetic industry
Messyasz, B., Łęska, B., Fabrowska, J., Pikosz, M., Rój, E., Cieślak, A., & Schroeder, G. (2015) · Open Chemistry · doi:10.1515/chem-2015-0124 · Cited by 66 · see citing works
“Higher efficiency of supercritical fluid extraction in obtaining bioactive substances from a natural sources as compared to other extraction methods has been proven in many studies [45,46].”
Jonas, A., Scholz, S., Fetter, É., Sychrová, E., Nováková, K., Ortmann, J., Beníšek, M., Adamovský, O., Giesy, J. P., & Hilscherová, K. (2014) · Chemosphere · doi:10.1016/j.chemosphere.2014.07.074 · Cited by 65 · see citing works
“Recent results have indicated the ability of compounds produced by cyanobacteria to interfere with signalling of several intracellular receptors, which play important roles in physiological processes and are of relevance for potential adverse effects in vertebrates including humans (Klejdus et al., 2010; Kaya et al.,…”
BackgroundGreen Analytical Methodologies for Preparation of Extracts and Analysis of Bioactive Compounds
Duarte, K. R., Justino, C. I. L., Gomes, A., Rocha‐Santos, T., & Duarte, A. C. (2014) · Comprehensive analytical chemistry · doi:10.1016/b978-0-444-63359-0.00004-5 · Cited by 64 · see citing works
In vivo UVB-photoprotective activity of extracts from commercial marine macroalgae
Guinea, M. T., Franco, V., Araújo‐Bazán, L., Rodríguez-Martín, I., & González, S. (2012) · Food and Chemical Toxicology · doi:10.1016/j.fct.2012.01.004 · Cited by 60 · see citing works
Microalgae as a Potential Source of Proteins
Matos, Â. P. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-816695-6.00003-9 · Cited by 62 · see citing works
Electrochemical Tools for Determination of Phenolic Compounds in Plants. A Review
Dobes, J., Zítka, O., Sochor, J., Ruttkay-Nedecký, B., Babula, P., Beklová, M., Kynický, J., Hubálek, J., Klejdus, B., Kizek, R., et al. (2013) · International Journal of Electrochemical Science · doi:10.1016/s1452-3981(23)14619-0 · Cited by 59 · see citing works
Green Extraction of Bioactive Compounds from Microalgae
Gallego, R., Montero, L., Cifuentes, A., Ibáñez, E., & Herrero, M. (2018) · Journal of Analysis and Testing · doi:10.1007/s41664-018-0061-9 · Cited by 59 · see citing works
Extraction of protocatechuic acid from Scutellaria barbata D. Don using supercritical carbon dioxide
Yang, Y., Wei, M., Huang, T., & Lee, S. (2013) · The Journal of Supercritical Fluids · doi:10.1016/j.supflu.2013.04.007 · Cited by 55 · see citing works
Sosa‐Hernández, J. E., Romero‐Castillo, K. D., Parra-Arroyo, L., Aguilar-Aguila-Isaías, M. A., García-Reyes, I. E., Ahmed, I., Parra‐Saldívar, R., Bilal, M., & Iqbal, H. M. N. (2019) · Marine Drugs · doi:10.3390/md17030174 · Cited by 56 · see citing works
“[69], it also allows for a more efficient extraction in freshwater algae species.”
BackgroundGreen extraction of biomolecules from algae using subcritical and supercritical fluids
Sarkar, S., Gayen, K., & Bhowmick, T. K. (2022) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-022-02309-3 · Cited by 53 · see citing works
“Extraction of other valuable compounds (e.g., isoflavones, hydrocarbon, vitamin E) also had been shown by using the SCCO 2 extraction process in different studies [29, 68].”
Supercritical fluid extraction of free amino acids from broccoli leaves
Arnáiz, E., Bernal, J., Martı́n, M. T., Nozal, M. J., Bernal, J., & Toribio, L. (2012) · Journal of Chromatography A · doi:10.1016/j.chroma.2012.04.066 · Cited by 51 · see citing works
Supercritical Algal Extracts: A Source of Biologically Active Compounds from Nature
Michalak, I., Dmytryk, A., Wieczorek, P. P., Rój, E., Łęska, B., Górka, B., Messyasz, B., Lipok, J., Mikulewicz, M., Wilk, R., et al. (2015) · Journal of Chemistry · doi:10.1155/2015/597140 · Cited by 51 · see citing works
Editorial: Interactive effects of UV-B radiation in a complex environment
Jansen, M. A. K., Bilger, W., Hideg, É., Strid, Å., Urban, O., Aphalo, P. J., Brelsford, C. C., Klem, K., Mátai, A., Llorens, L., et al. (2018) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2018.10.021 · Cited by 45 · see citing works
“found to produce isoflavones and its glycosides (Klejdus et al., 2010) and Arthrospira platensis contained 4′,5,7-trihydroxyflavone (Goiris et al.”
BackgroundUse of Microalgae Biomass for Fortification of Food Products from Grain
Bazarnova, J. G., NILOVA, L. P., Trukhina, E., Bernavskaya, M. V., Smyatskaya, Y. A., & Aktar, T. (2021) · Foods · doi:10.3390/foods10123018 · Cited by 44 · see citing works
“[62], microalgae synthesize isoflavones, flavanones, flavonols, and dihydrohalcones.”
Extraction of Polyphenols and Anthocyanins from the Jambul (Syzygium cumini) Fruit Peels
dos Santos, D. T., Cavalcanti, R. N., Rostagno, M. A., Queiroga, C. L., Eberlin, M. N., & Meireles, M. A. A. (2013) · Food and public health · doi:10.5923/j.fph.20130301.02 · Cited by 44 · see citing works
Procházková, T., Sychrová, E., Javůrková, B., Večerková, J., Kohoutek, J., Lepšová-Skácelová, O., Bláha, L., & Hilscherová, K. (2016) · Chemosphere · doi:10.1016/j.chemosphere.2016.12.006 · Cited by 42 · see citing works
Klejdus, B., Plaza, M., Šnóblová, M., & Lojková, L. (2016) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2016.12.015 · Cited by 43 · see citing works
The Potential of Algae in the Nutricosmetic Sector
Dini, I. (2023) · Molecules · doi:10.3390/molecules28104032 · Cited by 41 · see citing works
“) and brown seaweeds (Sargassum muticum and Sargassum vulgare) [189,190].”
BackgroundMarine Algal Polyphenols as Skin Protective Agents: Current Status and Future Prospectives
Jayawardhana, H. H. A. C. K., Jayawardena, T. U., Sanjeewa, K. K. A., Liyanage, N. M., Nagahawatta, D. P., Lee, H.-G., Kim, J., & Jeon, Y. (2023) · Marine Drugs · doi:10.3390/md21050285 · Cited by 44 · see citing works
Bioactive compounds from micro-algae and its application in foods: A review
Siddhnath, Surasani, V. K. R., Singh, A., Singh, S. M., Hauzoukim, Murthy, L. N., & Baraiya, K. G. (2024) · Discover Food · doi:10.1007/s44187-024-00096-6 · Cited by 42 · see citing works
Yang, Y., & Wei, M. (2015) · Industrial Crops and Products · doi:10.1016/j.indcrop.2015.10.038 · Cited by 39 · see citing works
Ultrasound pretreatment combined with supercritical CO2 extraction of Iberis amara seed oil
Liu, X., Ou, H., Xiang, Z., & Gregersen, H. N. (2020) · Journal of Applied Research on Medicinal and Aromatic Plants · doi:10.1016/j.jarmap.2020.100265 · Cited by 39 · see citing works
Wei, M., Yang, Y., Chiu, H., & Hong, S. (2013) · Journal of Chromatography B · doi:10.1016/j.jchromb.2013.09.015 · Cited by 37 · see citing works
Kapoor, S., Singh, M., Srivastava, A., Chavali, M. S., Chandrasekhar, K., & Verma, P. (2021) · Journal of Basic Microbiology · doi:10.1002/jobm.202100458 · Cited by 38 · see citing works
Yang, Y., Wei, M., Hong, S., Huang, T., & Lee, S. (2013) · Industrial Crops and Products · doi:10.1016/j.indcrop.2013.05.013 · Cited by 36 · see citing works
Yang, Y., & Wei, M. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.12.086 · Cited by 34 · see citing works
Bustamante-Rangel, M., Delgado-Zamarreño, M. M., Pérez‐Martín, L., & Carabias-Martı́nez, R. (2012) · Microchemical Journal · doi:10.1016/j.microc.2012.10.023 · Cited by 34 · see citing works
“The application of techniques such as ultrasound-assisted extraction (UAE) [11], microwave-assisted extraction (MAE) [12], pressurized liquid extraction (PLE) [13] or supercritical fluid extraction (SFE) [14] for the extraction of isoflavones from food samples offers advantages such as a reduction in extraction time,…”
MethodKanumuri, R. S., Kadian, N., Taneja, I., & Wahajuddin, M. (2015) · Phytochemistry Reviews · doi:10.1007/s11101-015-9400-x · Cited by 33 · see citing works
“Klejdus et al. (2010) developed a hyphenated ultrasound assisted super critical fluid extraction method for the extraction and determination of isoflavones in sea and freshwater algae and cyanobacteria .”
BackgroundMethodInfluentialKadam, S. U., Álvarez, C., Tiwari, B. K., & O’Donnell, C. P. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-418697-2.00004-0 · Cited by 32 · see citing works
Azim, N. H., Subki, A., & Yusof, Z. N. B. (2018) · Malaysian Journal of Microbiology · doi:10.21161/mjm.100317 · Cited by 32 · see citing works
Sigamani, S., Nandhagopal, M., Ragavendran, C., Mathivanan, N., Natarajan, D., Hemalatha, N., & Dhandapani, R. (2019) · Journal of Applied Microbiology · doi:10.1111/jam.14336 · Cited by 30 · see citing works
“Klejdus et al. (2010) have reported the presence of phytochemicals like flavonoids, glycosides, phenolic compounds, terpenoids and saponins from the methanol extracts of two microalgal strains.”
BackgroundBlooming cyanobacteria alter water flea reproduction via exudates of estrogen analogues
Xu, R., Jiang, Y., MacIsaac, H. J., Chen, L., Li, J., Xu, J., Wang, T., Zi, Y., & Chang, X. (2019) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2019.133909 · Cited by 30 · see citing works
“Therefore, their contributions to estrogenic effects should not be overlooked, especially considering the discovery of phytoestrogens in blooming cyanobacteria (Goiris et al., 2014; Klejdus et al., 2010; Procházková et al., 2017).”
BackgroundAstudillo-Pascual, M., Domínguez, I., Aguilera, P. A., & Frenich, A. G. (2021) · Plants · doi:10.3390/plants10050864 · Cited by 30 · see citing works
“To our knowledge it is also the first time t at these compounds have been reported in any marine seagrass, it being more common to find them in other samples such as leg mes and algae for biochanin A and glycitein, respectively [37,38].”
BackgroundPark, S., Sharma, A., & Lee, H. (2024) · Marine Drugs · doi:10.3390/md22010047 · Cited by 28 · see citing works
Castillo, A., Celeiro, M., Lores, M., Grgić, K., Banožić, M., Jerković, I., & Jokić, S. (2023) · Marine Drugs · doi:10.3390/md21020097 · Cited by 30 · see citing works
“Different isoflavones, such as daidzein or genistein, were identified in Sargassum muticum and Sargassum vulgare [15].”
An Overview of Nanoformulated Nutraceuticals and their Therapeutic Approaches
De, S., Agraharam, G., Keerthana, V., Girigoswami, A., & Girigoswami, K. (2020) · Current Nutrition & Food Science · doi:10.2174/1573401316999200901120458 · Cited by 27 · see citing works
Rahman, N. A., Katayama, T., Wahid, M. E. A., Kasan, N. A., Khatoon, H., Yamada, Y., & Takahashi, K. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.581628 · Cited by 27 · see citing works
Determination of Phytoestrogen Content in Fresh-Cut Legume Forage
Hloučalová, P., Skládanka, J., Horký, P., Klejdus, B., Pelikán, J., & Knotová, D. (2016) · Animals · doi:10.3390/ani6070043 · Cited by 27 · see citing works
Seaweeds: a nutraceutical and health food
Hayes, M. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-418697-2.00014-3 · Cited by 26 · see citing works
Prochazkova, T., Sychrová, E., Večerková, J., Javůrková, B., Otoupalíková, A., Pernica, M., Šimek, Z., Smutná, M., Lepšová-Skácelová, O., & Hilscherová, K. (2018) · Water Research · doi:10.1016/j.watres.2018.02.040 · Cited by 22 · see citing works
Tsiaka, T., Sinanoglou, V. J., & Zoumpoulakis, P. G. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-811521-3.00008-9 · Cited by 22 · see citing works
Green analytical methodologies for the discovery of bioactive compounds from marine sources
Duarte, K., Justino, C. I. L., Pereira, R., Freitas, A. C., Gomes, A. M., Duarte, A. C., & Rocha-Santos, T. A. P. (2014) · Trends in Environmental Analytical Chemistry · doi:10.1016/j.teac.2014.11.001 · Cited by 21 · see citing works
Zayed, A., El-Seadawy, H. M., Attia, E. Z., Rushdi, M. I., & Abdelmohsen, U. R. (2024) · Frontiers in Natural Products · doi:10.3389/fntpr.2024.1425242 · Cited by 22 · see citing works
Microalgae as a Novel Source of Antioxidants for Nutritional Applications
Goiris, K., Muylaert, K., & Cooman, L. D. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-800776-1.00017-0 · Cited by 21 · see citing works
Advanced Extraction Processes to Obtain Bioactives from Marine Foods
Plaza, M., & Rodríguez‐Meizoso, I. (2013) · Wiley eBooks · doi:10.1002/9781118412893.ch16 · Cited by 21 · see citing works
Gager, L., Lalegerie, F., Connan, S., & Stiger‐Pouvreau, V. (2021) · Wiley eBooks · doi:10.1002/9781119542650.ch11 · Cited by 21 · see citing works
Khalil, A. H., Aidy, E. A., Said, M. A., Kebeish, R., & Al-Badwy, A. H. (2024) · Algal Research · doi:10.1016/j.algal.2024.103624 · Cited by 20 · see citing works
Fan, L., Jin, R., Liu, Y., An, M., & Chen, S. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.09.035 · Cited by 20 · see citing works
Shetty, V., & Sibi, G. (2014) · Journal of Food Resource Science · doi:10.3923/jfrs.2015.1.9 · Cited by 20 · see citing works
“A number of phenolic compounds are present in microalgae (Klejdus et al., 2010; Kovacik et al., 2010) and were reported to responsible for microalgal antioxidant properties.”
BackgroundDeniz, İ., García‐Vaquero, M., & İmamoğlu, E. (2017) · Elsevier eBooks · doi:10.1016/b978-0-08-101023-5.00018-2 · Cited by 20 · see citing works
Radwan, A. M., Ahmed, E., Donia, A. E. R. M., Mustafa, A. E., & Balah, M. A. A. (2023) · Scientific Reports · doi:10.1038/s41598-023-38711-8 · Cited by 19 · see citing works
Benedé, J. L., Chisvert, A., Lucena, R., & Cárdenas, S. (2021) · Talanta · doi:10.1016/j.talanta.2021.122582 · Cited by 18 · see citing works
Sushanth, V. R., & Rajashekhar, M. (2015) · Cited by 18 · see citing works
Mosaddik, A., Ravinayagam, V., Elaanthikkal, S., Fessi, H., Badri, W., & Elaı̈ssari, A. (2018) · Springer eBooks · doi:10.1007/978-3-030-00545-0_11 · Cited by 18 · see citing works
Visnevschi‐Necrasov, T., Barreira, J. C. M., Cunha, S. C., Pereira, G., Nunes, E., & Oliveira, M. B. P. P. (2014) · Phytochemical Analysis · doi:10.1002/pca.2534 · Cited by 18 · see citing works
“…Soxhlet extraction, ultrasound-assisted solvent extraction, Wiley & Sons, Ltd. microwave-assisted solvent extraction, accelerated solvent extraction and supercritical fluid extraction, have already been used for isolation of phytochemicals (Klejdus et al., 2004, 2010; Oniszczuk et al., 2013).”
MethodFasciotti, M., Souza, G. H. M. F., Astarita, G., Costa, I. C. R., Monteiro, T. V. C., Teixeira, C. M. L. L., Eberlin, M. N., & Sarpal, A. S. (2019) · Analytical Chemistry · doi:10.1021/acs.analchem.9b02172 · Cited by 15 · see citing works
Supercritical CO2 extraction of total flavonoids from Iberis amara assisted by ultrasound
Liu, X., Ou, H., Zuo, J., & Gregersen, H. N. (2022) · The Journal of Supercritical Fluids · doi:10.1016/j.supflu.2022.105581 · Cited by 16 · see citing works
- Authors
- Klejdus, B., Lojková, L., Plaza, M., Šnóblová, M., & Štěrbová, D.
- Affiliations
- Mendel University in Brno (CZ)
- Consejo Superior de Investigaciones Científicas (ES)
- Corresponding author
- Klejdus, B.
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 2010-12
- Volume
- 1217
- Issue
- 51
- Pages
- 7956-7965
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W2008516333
- Cited by
- 182
- References
- 24
- Citations by year
- 2026: 8 · 2025: 11 · 2024: 10 · 2023: 19 · 2022: 12 · 2021: 19 · 2020: 12 · 2019: 16 · 2018: 11 · 2017: 5 · 2016: 4 · 2015: 13 · 2014: 13 · 2013: 15 · 2012: 9
- FWCI
- 13.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography A (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Grantová Agentura České Republiky
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical chemistry methods development
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Keywords
- isoflavones, algae, cyanobacteria, ultrasonic pretreatment, isoflavone extraction, seaweed, freshwater algae, isoflavone quantification, extraction time optimization
- MeSH terms
- Phaeophyceae, Rhodophyta, Chromatography, Liquid, Isoflavones, Ultrasonics, Chromatography, Supercritical Fluid, Tandem Mass Spectrometry
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
