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Safety aspects of next generation probiotics
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“Computational tools can be applied to mitigate risks associated with NGPs by enabling whole-genome sequence analysis, early detection of antibiotic resistance genes, virulence-related traits, the potential for horizontal gene transfer, and other potentially negative metabolic characteristics, thus speeding up strain selection and regulatory assessment [140,161].”
From Current Concepts in Probiotic Safety and Efficacy (2026) · cites this paper as [140]
In the citing paper
“Another class of probiotics, NGPs (e.g., specifically commensal gut bacteria such as Bacteroides, Clostridium, and Akkermansia), require greater safety consideration than traditional probiotics because they lack a “safe use history” and have a more “ambiguous” potential to cause infectious diseases [140].”
From Current Concepts in Probiotic Safety and Efficacy (2026) · cites this paper as [140]
Citations
Cites
Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity
Everard, A., Belzer, C., Geurts, L., Ouwerkerk, J. P., Druart, C., Bindels, L. B., Guiot, Y., Derrien, M., Muccioli, G. G., Delzenne, N. M., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1219451110 · Cited by 4,511
Bacteroides : the Good, the Bad, and the Nitty-Gritty
Wexler, H. M. (2007) · Clinical Microbiology Reviews · doi:10.1128/cmr.00008-07 · Cited by 2,388
Plovier, H., Everard, A., Druart, C., Depommier, C., Van Hul, M., Geurts, L., Chilloux, J., Ottman, N., Duparc, T., Lichtenstein, L., et al. (2017) · Nature Medicine · doi:10.1038/nm.4236 · Cited by 2,204
Akkermansia muciniphila gen. nov., sp. nov., a human intestinal mucin-degrading bacterium
Derrien, M., Vaughan, E. E., Plugge, C. M., & de Vos, W. M. (2004) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/ijs.0.02873-0 · Cited by 2,094
Akkermansia muciniphila and its role in regulating host functions
Derrien, M., Belzer, C., & de Vos, W. M. (2017) · Microbial Pathogenesis · doi:10.1016/j.micpath.2016.02.005 · Cited by 1,044
Next-generation probiotics: the spectrum from probiotics to live biotherapeutics
O’Toole, P. W., Marchesi, J. R., & Hill, C. (2017) · Nature Microbiology · doi:10.1038/nmicrobiol.2017.57 · Cited by 971
Shoemaker, N. B., Vlamakis, H. C., Hayes, K., & Salyers, A. A. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.2.561-568.2001 · Cited by 529
Hansen, C. H. F., Krych, Ł., Nielsen, D. S., Vogensen, F. K., Hansen, L. H., Sørensen, S. J., Buschard, K., & Hansen, A. K. (2012) · Diabetologia · doi:10.1007/s00125-012-2564-7 · Cited by 507
van Passel, M. W. J., Kant, R., Zoetendal, E. G., Plugge, C. M., Derrien, M., Malfatti, S., Chain, P., Woyke, T., Palva, A., de Vos, W. M., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016876 · Cited by 430
Martín, R., Miquel, S., Benevides, L., Bridonneau, C., Robert, V., Hudault, S., Chain, F., Berteau, O., Azevedo, V., Chatel, J. M., et al. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01226 · Cited by 398
Research works printed but not linked: 2
- Turck, 2016, Guidance on the preparation and presentation of an application for authorisation of a novel food in the context of Regulation (EU) 5/2283 EFSA, EFSA J, 14, 4594
- Chang, 2016, Modulation of host immune response by Bacteroides fragilis polysaccharides: a review of recent observation, J Biomed Lab Sci, 28, 1
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Gut microbes and metabolites as modulators of blood-brain barrier integrity and brain health
Parker, A., Fonseca, S., & Carding, S. R. (2019) · Gut Microbes · doi:10.1080/19490976.2019.1638722 · Cited by 745
“There is increasing interest in exploring the beneficial features of next-generation probiotics or live therapeutic products which include species not traditionally considered as probiotics, such as Akkermansia muciniphila,(187) Faecalibacterium prausnitzii,(188) species of the genus Bacteroides,(189) and genetically…”
BackgroundHelmy, Y. A., Taha-Abdelaziz, K., Hawwas, H. A. E.-H., Ghosh, S. B., Alkafaas, S. S., Moawad, M. M. M., Saied, E. M., Kassem, I. I., & Mawad, A. M. M. (2023) · Antibiotics · doi:10.3390/antibiotics12020274 · Cited by 253
“prausnitzii have been related to its potential health benefits [177].”
BackgroundInfluentialNext-generation probiotics: the upcoming biotherapeutics
Al-Fakhrany, O. M., & Elekhnawy, E. (2024) · Molecular Biology Reports · doi:10.1007/s11033-024-09398-5 · Cited by 208
“Most of these microbes have metabolic activities that make them challenging to use in large-scale food production [73, 74].”
BackgroundPerraudeau, F., McMurdie, P., Bullard, J., Cheng, A., Cutcliffe, C., Deo, A., Eid, J., Gines, J., Iyer, M., Justice, N., et al. (2020) · BMJ Open Diabetes Research & Care · doi:10.1136/bmjdrc-2020-001319 · Cited by 186
Probiotics-based foods and beverages as future foods and their overall safety and regulatory claims
Koirala, S., & Anal, A. K. (2021) · Future Foods · doi:10.1016/j.fufo.2021.100013 · Cited by 181
Roles of intestinal bacteroides in human health and diseases
Wang, C., Zhao, J., Zhang, H., Lee, Y. K., Zhai, Q., & Chen, W. (2020) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2020.1802695 · Cited by 169
“A recent review (Saarela 2019) indicated that the assessment of the NGP should include (i) correct identification of the strain, (ii) whole genome sequence based analysis of safety and confirmation of the identity, (iii) regulatory issues, (iv) growth experiments, (v) in vitro tests for safety,…”
BackgroundNext-generation probiotics: a promising approach towards designing personalized medicine
Singh, T. P., & Nataraj, B. H. (2021) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2021.1902940 · Cited by 150
“Considering the safety aspects of the potential NGP, genomic analysis for the presence of virulence and drug resistance traits as well as acute toxicity study in both healthy and immunosuppressed mice must be investigated (Saarela 2019; Tan et al. 2020).”
BackgroundNyanzi, R., Jooste, P. J., & Buys, E. M. (2021) · Journal of Dairy Science · doi:10.3168/jds.2020-19116 · Cited by 137
Considerations for determining safety of probiotics: A USP perspective
Roe, A. L., Boyte, M.-E., Elkins, C. A., Goldman, V. S., Heimbach, J., Madden, E., Oketch-Rabah, H., Sanders, M. E., Sirois, J., & Smith, A. (2022) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2022.105266 · Cited by 136
“2 have been proposed and published by others (Pariza et al., 2015; Brodmann et al., 2017; EFSA, 2017; Saarela, 2019; Pradhan et al., 2020; Rouanet et al., 2020).”
BackgroundNext-generation probiotics – do they open new therapeutic strategies for cancer patients?
Kaźmierczak-Siedlecka, K., Skonieczna‐Żydecka, K., Hupp, T., Duchnowska, R., Marek-Trzonkowska, N. M., & Połom, K. (2022) · Gut Microbes · doi:10.1080/19490976.2022.2035659 · Cited by 137
“According to recently published data, NGPs groups include mainly Faecalibacterium prausnitzii, Bacteroides fragilis, Akkermansia muciniphila, Prevotella copri, Bacteroides thetaiotaomicron, Christensenella minuta, and Parabacteroides goldsteinii.(11,12,15) However, most of them are not related to cancer/or their prope…”
BackgroundProbiotic-directed modulation of gut microbiota is basal microbiome dependent
Hou, Q., Zhao, F., Liu, W., Lv, R., Khine, W. W. T., Han, J., Sun, Z., Lee, Y. K., & Zhang, H. (2020) · Gut Microbes · doi:10.1080/19490976.2020.1736974 · Cited by 127
Kumari, M., Singh, P., Nataraj, B. H., Kokkiligadda, A., Naithani, H., Ali, S. A., Behare, P. V., & Nagpal, R. (2021) · Food Research International · doi:10.1016/j.foodres.2021.110716 · Cited by 113
Balthazar, C. F., Guimarães, J. T., Coutinho, N. M., Pimentel, T. C., Ranadheera, C. S., Santillo, A., Albenzio, M., da Cruz, A. G., & Sant’Ana, A. S. (2022) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12962 · Cited by 108
Role of prebiotics in enhancing the function of next-generation probiotics in gut microbiota
Fei, Y., Chen, Z., Han, S., Zhang, S., Zhang, T., Lu, Y., Berglund, B., Xiao, H., Li, L., & Yao, M. (2021) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2021.1958744 · Cited by 105
“Most NGPs are anaerobes and oxygen exposure is often a threat their survival during processing and storage (Saarela 2019).”
BackgroundEpigenetics in depression and gut-brain axis: A molecular crosstalk
Begum, N., Mandhare, A., Tryphena, K. P., Srivastava, S., Shaikh, M. F., Singh, S. B., & Khatri, D. K. (2022) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2022.1048333 · Cited by 102
“Their use in probiotic products is increasing as well (Saarela, 2019).”
BackgroundRole of Akkermansia in Human Diseases: From Causation to Therapeutic Properties
Pellegrino, A., Coppola, G., Santopaolo, F., Gasbarrini, A., & Ponziani, F. R. (2023) · Nutrients · doi:10.3390/nu15081815 · Cited by 107
Investiture of next generation probiotics on amelioration of diseases – Strains do matter
Lin, T., Shu, C.-C., Lai, W.-F., Tzeng, C., Lai, H., & Lu, C. (2019) · Medicine in Microecology · doi:10.1016/j.medmic.2019.100002 · Cited by 101
Evolving trends in next-generation probiotics: a 5W1H perspective
Almeida, D., Machado, D., Andrade, J. C., Mendo, S., Gomes, A. M., & Freitas, A. C. (2020) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2019.1599812 · Cited by 92
“Nevertheless, the inherent novelty to NGP also entails little information about the safety and the recommended dosage for its supplementation, since this parameter changes with the probiotic strain involved (Saarela 2018).”
BackgroundKrzyżek, P., Marinacci, B., Vitale, I., & Grande, R. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15020522 · Cited by 91
A Critical Perspective on the Supplementation of Akkermansia muciniphila: Benefits and Harms
Chiàntera, V., Laganà, A. S., Basciani, S., Nordio, M., & Bizzarri, M. (2023) · Life · doi:10.3390/life13061247 · Cited by 91
“, Clostridium butyricum, Faecalibacterium prausnitzii), however, all these genera and microbial species do not have a history of safe use yet [58,59].”
BackgroundBeneficial microbiota. Probiotics and pharmaceutical products in functional nutrition and medicine
Novik, G. I., & Savich, V. V. (2019) · Microbes and Infection · doi:10.1016/j.micinf.2019.06.004 · Cited by 79
Probiotic and postbiotic analytical methods: a perspective of available enumeration techniques
Boyte, M.-E., Benkowski, A., Pane, M., & Shehata, H. R. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1304621 · Cited by 83
“…probiotic taxa like Bifidobacterium spp. and, Lactobacillus spp., the industry is confronted with additional challenges when enumerating novel microorganisms, often referred to as Next-Generation Probiotics (NGPs) (O’Toole et al., 2017; Saarela, 2019; Singh and Natraj, 2021; Torp et al., 2022).”
BackgroundMethodInfluentialAlternative therapeutics to control antimicrobial resistance: a general perspective
Singha, B., Singh, V., & Soni, V. (2024) · Frontiers in Drug Discovery · doi:10.3389/fddsv.2024.1385460 · Cited by 83
Kan, X., Hu, Y., Huang, Y., Fan, X., Chen, G., Ye, H., & Zeng, X. (2022) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2022.108060 · Cited by 67
Emerging pre-clinical safety assessments for potential probiotic strains: a review
Nataraj, B. H., Behare, P. V., Yadav, H., & Srivastava, A. K. (2023) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2023.2197066 · Cited by 72
“An escalating shift from traditional probiotics to next-generation probiotics also demands an initial idea of safety perspectives (Saarela 2019).”
Functional meat products: Trends in pro-, pre-, syn-, para- and post-biotic use
Manassi, C. F., de Souza, S. S., Hassemer, G. D. S., Sartor, S., Lima, C. M. G., Miotto, M., Lindner, J. D. D., Rezzadori, K., Pimentel, T. C., Ramos, G. L. D. P. A., et al. (2022) · Food Research International · doi:10.1016/j.foodres.2022.111035 · Cited by 69
Shen, X., Xie, A., Li, Z., Jiang, C., Wu, J., Li, M., & Yue, X. (2024) · Foods · doi:10.3390/foods13010151 · Cited by 67
“…responsible for their bene fi cial e ff ects can help to maintain the equilibrium of the gut microbiota; this includes boosting the population of bene fi cial bacteria in the intestinal ecosystem while decreasing the abundance of harmful bacteria, ultimately bolstering intestinal immunity [61].”
BackgroundExploring the Probiotic Potential of Bacteroides spp. Within One Health Paradigm
Tufail, M. A., & Schmitz, R. A. (2024) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-024-10370-9 · Cited by 65
“…probiotics Highlights the importance of gut microbiota for health and assesses safety of new probiotics concerning antibiotic resistance Saarela [129] Roles of intestinal bacteroides in human health and diseases Emphasizes the role of dietary nutrition on the intestinal microbiome and debates…”
Bacillus coagulans Unique IS2 in Constipation: A Double-Blind, Placebo-Controlled Study
Madempudi, R. S., Neelamraju, J., Ahire, J. J., Gupta, S. K., & Shukla, V. K. (2020) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-019-09542-9 · Cited by 60
Innovation and Trends in Probiotic Microencapsulation by Emulsification Techniques
Camelo‐Silva, C., Verruck, S., Ambrosi, A., & Luccio, M. D. (2022) · Food Engineering Reviews · doi:10.1007/s12393-022-09315-1 · Cited by 56
“Nevertheless, many challenges need to be overcome, like the need for higher efficacy and higher safety for consumption of these new probiotics [60], as well as the technological aspects of the incorporation of these microorganisms in food.”
BackgroundIntegrated genome-based probiotic relevance and safety evaluation of Lactobacillus reuteri PNW1
Alayande, K. A., Aiyegoro, O. A., Nengwekhulu, T. M., Katata‐Seru, L., & Ateba, C. N. (2020) · PLoS ONE · doi:10.1371/journal.pone.0235873 · Cited by 57
Personalized Nutrition Through The Gut Microbiota: Current Insights And Future Perspectives
Vandeputte, D. (2020) · Nutrition Reviews · doi:10.1093/nutrit/nuaa098 · Cited by 63
Niamah, A. K., Al‐Sahlany, S. T. G., Ibrahim, S. A., Verma, D. K., Thakur, M., Sıngh, S., Patel, A. R., Aguilar, C. N., & Utama, G. L. (2021) · Food Bioscience · doi:10.1016/j.fbio.2021.101458 · Cited by 53
Probiotic Bacillus as fermentation agents: Status, potential insights, and future perspectives
Liu, S., Zhao, L., Li, M., Zhu, Y., Liang, D., Ma, Y., Sun, L., Zhao, G., & Tu, Q. (2024) · Food Chemistry X · doi:10.1016/j.fochx.2024.101465 · Cited by 53
“Therefore, in-depth and continuous exploration of probiotic strains with good fermentation performance is necessary for the industrialized development of traditional fermented foods (Saarela, 2019).”
BackgroundHuman Gut Microbiota for Diagnosis and Treatment of Depression
Averina, O. V., Poluektova, E. U., Zorkina, Y., Kovtun, A. S., & Даниленко, В. Н. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25115782 · Cited by 54
“Such probiotics are currently under intensive study [215,216].”
BackgroundKorotkyi, O., Huet, A., Dvorshchenko, K., Kobyliak, N. M., Falalyeyeva, T. M., & Остапченко, Л. І. (2021) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-020-09735-7 · Cited by 45
Probiotics in human mental health and diseases—A minireview
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“Next-generation probiotics are novel microbes the human GM that are associated with health and have no history of usage in the food industry [46].”
BackgroundFan, S., Zhang, Z., Zhao, Y., Daglia, M., Zhang, J., Zhu, Y., Bai, J., Zhu, L., & Xiao, X. (2022) · Current Opinion in Food Science · doi:10.1016/j.cofs.2022.100959 · Cited by 41
Safety assessment of Lactobacillus reuteri IDCC 3701 based on phenotypic and genomic analysis
Lee, B. S., Ban, O., Bang, W. Y., Chae, S. A., Oh, S., Park, C., Lee, M., Kim, S.-J., Yang, J., & Jung, Y. H. (2021) · Annals of Microbiology · doi:10.1186/s13213-021-01622-y · Cited by 38
“Probiotics have recently begun to be considered as one of the most effective sources of functional foods (Byakika et al. 2019; Saarela 2019).”
BackgroundFruit bioactive compounds: Effect on lactic acid bacteria and on intestinal microbiota
Borgonovi, T. F., Virgolin, L. B., Janzantti, N. S., Casarotti, S. N., & Penna, A. L. B. (2022) · Food Research International · doi:10.1016/j.foodres.2022.111809 · Cited by 40
Probiotic Functional Yogurt: Challenges and Opportunities
de Souza, M., Drunkler, D. A., & Colla, E. (2023) · Fermentation · doi:10.3390/fermentation10010006 · Cited by 36
Chakravarty, K., Gaur, S., Kumar, R., Jha, N. K., & Gupta, P. K. (2024) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-024-10328-x · Cited by 41
“Certain enzymes linked to pathogenesis, such as neuraminidase, collagenase, and hyaluronidase, must not be present in a potential probiotic organism [212].”
InfluentialUnderstanding the Functionality of Probiotics on the Edge of Artificial Intelligence (AI) Era
Asar, R., Erenler, S., Devecioglu, D., Ispirli, H., Karbancioglu-Guler, F., Ozturk, H. I., & Dertli, E. (2025) · Fermentation · doi:10.3390/fermentation11050259 · Cited by 37
“Another critical factor is that NGP production is somewhat more complex than other alternatives [79].”
Korotkyi, O., Vovk, A., Галенова, Т. І., Vovk, T. B., Dvorshchenko, K., Luzza, F., Abenavoli, L., Kobyliak, N. M., Falalyeyeva, T. M., & Остапченко, Л. І. (2019) · Minerva Biotecnologica · doi:10.23736/s1120-4826.19.02548-5 · Cited by 34
Rodrigues, V. C. D. C., Duque, A. L. R. F., Fino, L. D. C., Simabuco, F. M., Sartoratto, A., Cabral, L., Noronha, M. F., Sivieri, K., & Antunes, A. E. C. (2020) · British Journal Of Nutrition · doi:10.1017/s0007114520000896 · Cited by 34
“Next-generation probiotics, also known as live therapeutic products, are potentially probiotic species or genera not used by the food industry yet(94).”
BackgroundRole of fructooligosaccharides in promoting beneficial gut bacteria: A Prebiotic perspective
Singh, P., Gupta, S., Kundu, A., Grover, M., & Saha, S. (2024) · Food Bioscience · doi:10.1016/j.fbio.2024.105726 · Cited by 36
Next Generation Probiotics for Neutralizing Obesogenic Effects: Taxa Culturing Searching Strategies
López‐Moreno, A., Acuña, I., Torres-Sánchez, A., Ruiz-Moreno, Á., Cerk, K., Rivas, A., Suárez, A., Monteoliva‐Sánchez, M., & Aguilera, J. (2021) · Nutrients · doi:10.3390/nu13051617 · Cited by 35
Lalowski, P., & Zielińska, D. (2024) · Fermentation · doi:10.3390/fermentation10090444 · Cited by 30
Wang, C., Guo, H., Bai, J., Yu, L., Tian, F., Zhao, J., Zhang, H., Chen, W., & Zhai, Q. (2024) · Food & Function · doi:10.1039/d3fo04863b · Cited by 29
Mohr, A. E., Pugh, J., O'Sullivan, O., Black, K., Townsend, J. R., Pyne, D. B., Wardenaar, F. C., West, N. P., Whisner, C. M., & McFarland, L. V. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.809983 · Cited by 27
“Potential candidates are represented by microbial genera and species that have not been used in the food industry (27).”
BackgroundGut Microbiota Modulation in IBD: From the Old Paradigm to Revolutionary Tools
Murgiano, M., Bartocci, B., Puca, P., Vincenzo, F. D., GAUDIO, A. D., Papa, A., Cammarota, G., Gasbarrini, A., Scaldaferri, F., & Lopetuso, L. R. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26073059 · Cited by 30
“Safety is a critical factor in NGP development [171], as long-term exposure to probiotics may alter microbial functions or negatively impact the surrounding microbiota [172].”
Combined effects of probiotic and chondroprotector during osteoarthritis in rats
Korotkyi, O., Dvorshchenko, K., Falalyeyeva, T. M., Sulaieva, O., Kobyliak, N. M., Abenavoli, L., Fagoonee, S., Pellicano, R., & Остапченко, Л. І. (2020) · Panminerva Medica · doi:10.23736/s0031-0808.20.03841-0 · Cited by 26
Characterization of antibiotic-resistance traits in Akkermansia muciniphila strains of human origin
Filardi, R., Gargari, G., Mora, D., & Arioli, S. (2022) · Scientific Reports · doi:10.1038/s41598-022-23980-6 · Cited by 26
The Use of Probiotic Therapy in Metabolic and Neurological Diseases
Lee, S. H. F., Ahmad, S. R., Lim, Y. C., & Zulkipli, I. N. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.887019 · Cited by 26
“However, recent studies have identified other species of bacteria that may also confer benefits when used as probiotics, such as Akkermansia muciniphila and Faecalibacterium prausnitzii (25), and the use of these bacteria in probiotic products is rising as well (26).”
BackgroundFu, C. M., Shah, A. A., Khan, R. U., Khan, M. S., & Wanapat, M. (2023) · Microbial Pathogenesis · doi:10.1016/j.micpath.2023.106290 · Cited by 25
Pan, Q., Cen, S., Yu, L., Tian, F., Zhao, J., Zhang, H., Chen, W., & Zhai, Q. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.615876 · Cited by 24
“Considering the potential HGT of virulence factors, it is necessary to reevaluate the safety of commonly recognized probiotics (Saarela, 2019; Sotoudegan et al., 2019).”
BackgroundMa, C., Zhang, C., Chen, D., Jiang, S., Shen, S., Huo, D., Huang, S. W., Zhai, Q., & Zhang, J. (2021) · Communications Biology · doi:10.1038/s42003-021-02724-8 · Cited by 24
Sadeghi, A., Ebrahimi, M. T., Kharazmi, M. S., & Jafari, S. M. (2023) · Food Bioscience · doi:10.1016/j.fbio.2023.102843 · Cited by 24
Current Concepts in Probiotic Safety and Efficacy
Churin, A. A., Sokolyanskaya, L. O., Lukina, A. P., & Karnachuk, O. V. (2026) · Nutrients · doi:10.3390/nu18040696 · Cited by 31
“…of probiotics, NGPs (e.g., specifically commensal gut bacteria such as Bacteroides , Clostridium , and Akkermansia ), require greater safety consideration than traditional probiotics because they lack a “safe use history” and have a more “ambiguous” potential to cause infectious diseases [140].”
A Review of Next-Generation Probiotics—As a Gateway to Biotherapeutics
Vijayaganapathi, A., & Mohanasrinivasan, V. (2025) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-025-10606-2 · Cited by 25
“…assessment include clear taxonomic classification at the species level, in-depth strain characterization using whole-genome sequencing analysis to identify virulence-related genes, antibiotic resistance and its possible horizontal transfer, and other potentially hazardous metabolic features [91].”
Puntillo, M. A., Segli, F., Champagne, C. P., Raymond, Y., & Vinderola, G. (2022) · Annual Review of Food Science and Technology · doi:10.1146/annurev-food-052720-011545 · Cited by 22
Cerk, K., & Aguilera, J. (2022) · EFSA Journal · doi:10.2903/j.efsa.2022.e200404 · Cited by 21
“It is important to characterise in vitro bacterial physiology, genomic analysis of potential virulence and antimicrobial resistance genes, investigations on the presence or absence of potential genes involved in transferring antibiotic resistance gene, and in vivo acute toxicity study in both healthy and immunosuppres…”
BackgroundThe impact of novel probiotics isolated from the human gut on the gut microbiota and health
Caesar, R. (2024) · Diabetes Obesity and Metabolism · doi:10.1111/dom.16129 · Cited by 22
“87 This process also allows precise strain identification, functional assessment, and compliance with regulatory standards.”
InfluentialBorrero‐López, A. M., Valencia, C., Blánquez, A., Hernández, M., Eugenio, M. E., & Franco, J. M. (2020) · Polymers · doi:10.3390/polym12122822 · Cited by 20
Isolation and identification of a human intestinal bacterium capable of daidzein conversion
Guo, Y., Zhao, L., Fang, X., Zhong, Q., Liang, H., Liang, W., & Wang, L. (2021) · FEMS Microbiology Letters · doi:10.1093/femsle/fnab046 · Cited by 19
Next-Generation Probiotics - The Future of Biotherapeutics
(2022) · Microbial Bioactives · doi:10.25163/microbbioacts.514309 · Cited by 21
“The great majority of these microorganisms have metabolic properties that make them challenging to utilize as sustainable methods in the development of novel products, particularly in large-scale food production (O'toole et al., 2017; Saarela, 2019).”
BackgroundRibeiro, T. S., Sampaio, K. B., Menezes, F. N. D. D., de Assis, P. O. A., Lima, M. D. S., de Oliveira, M. E. G., de Souza, E. L., & Queiroga, R. D. C. R. D. E. (2020) · 3 Biotech · doi:10.1007/s13205-020-02442-8 · Cited by 20
“2017; Saarela 2019).”
Yang, T., Du, M., Zhang, J., Ahmad, B., Cheng, Q., Wang, X., Abbas, Z., Tong, Y., Li, J., Zhou, Y., et al. (2023) · Antibiotics · doi:10.3390/antibiotics12030433 · Cited by 17
“butyricum, which facilitates intestinal epithelium growth [44].”
Torres-Sánchez, A., Ruiz-Rodriguez, A., Ortíz, P., Moreno, M. A., Ampatzoglou, A., Gruszecka‐Kosowska, A., Monteoliva‐Sánchez, M., & Aguilera, J. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232112917 · Cited by 19
Research trends of next generation probiotics
Hasnain, M. A., Kang, D., & Moon, G. (2024) · Food Science and Biotechnology · doi:10.1007/s10068-024-01626-9 · Cited by 17
“Faecalibacterium prausnitzii, Bacteroides fragilis, Akker-mansia muciniphila are among the most widely anticipated NGPs (Chang et al., 2019; Kaźmierczak-Siedlecka et al., 2020; Saarela, 2019).”
Bede, D., & Lou, Z. (2021) · Food Reviews International · doi:10.1080/87559129.2021.1939368 · Cited by 15
“Lactobacillus and Bifidobacterium are the main gram-positive genera currently classified as probiotics in most products sold on the market.([70]) In the food sector, different species of probiotic microorganisms such as: Lactobacillus acidophilus, L.”
BackgroundBarbosa, J. C., Machado, D., Almeida, D., Andrade, J. C., Brandelli, A., Gomes, A., & Freitas, A. C. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-85170-1.00012-9 · Cited by 13
Pereira, L. J. R., & Monteiro, R. (2022) · Clinical Nutrition ESPEN · doi:10.1016/j.clnesp.2022.08.002 · Cited by 13
Probiotics in fermented products and supplements
Baliyan, N., Kumari, M., Kumari, P., Dindhoria, K., Mukhia, S., Kumar, S., Gupta, M., & Kumar, R. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-823506-5.00014-x · Cited by 12
Microbiological quality of probiotic products
Zavišić, G., Ristić, S., Petković, B., Živkov-Šaponja, D., Jojić, N., & Janković, D. (2023) · Arhiv za farmaciju · doi:10.5937/arhfarm73-42160 · Cited by 9
Trends and challenges for the application of probiotic lactic acid bacteria in functional foods
Silva, A. S., Casarotti, S. N., & Penna, A. L. B. (2023) · Ciência Rural · doi:10.1590/0103-8478cr20230014 · Cited by 8
Overall Safety Considerations and Regulatory Oversight for Probiotics‐based Foods and Beverages
Koirala, S., Thapa, S. P., & Anal, A. K. (2022) · doi:10.1002/9781119702160.ch18 · Cited by 8
Conventional versus emerging techniques in probiotic enumeration: a comprehensive review
Sun, X., Zhang, J., Chen, J., Yang, L., Pang, X., Fan, Y., Chen, X., Chen, K., Liu, C., Li, X., et al. (2025) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2025.2527355 · Cited by 6
Next-Generation Probiotics Their Molecular Taxonomy and Health Benefits
Khan, S. T., & Malik, A. R. A. (2019) · Springer eBooks · doi:10.1007/978-3-030-24903-8_16 · Cited by 7
Probiotics, Microbiome and the Concept of Cross-Feeding
Torres-Miranda, A., Vega, M. A., & Garrido, D. A. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-819265-8.00055-3 · Cited by 7
A next generation probiotic, Bacteroides xylanisolvens
Chen, C., Nie, Q., Lin, Q., Zou, J., & Nie, S. (2025) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2025.105173 · Cited by 5
Current Advancements of Probiotic Foods and Their Role in Sustainable Food Security
Yaekob, A. T., Gebreslassie, G., Mesele, E., & Semere, T. (2026) · Food Bioengineering · doi:10.1002/fbe2.70046 · Cited by 8
Introduction to Probiotics and Their Potential Health Benefits
El-Dalatony, M. M., & Li, X. (2020) · Springer eBooks · doi:10.1007/978-981-15-4759-1_3 · Cited by 5
Kumari, M., & Kokkiligadda, A. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-822909-5.00004-6 · Cited by 5
Allahverdi, M., & Dadmehr, M. (2026) · Journal of Food Composition and Analysis · doi:10.1016/j.jfca.2026.108917 · Cited by 10
Exploring Enterococcus Species for their Next-Generation Probiotics Potential
Hussain, A., & Ali, S. A. (2024) · IntechOpen eBooks · doi:10.5772/intechopen.1007306 · Cited by 4
Kang, M., Jo, J., Shin, H., & Kang, H. W. (2025) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2025.113201 · Cited by 3
Soliman, N. S., Soliman, M. S., Aziz, H. S. A., & El‐Kholy, A. (2025) · Scientific Reports · doi:10.1038/s41598-025-22788-4 · Cited by 3
“…apparent predominance of other species, notably Faecalibacter prausnitzii , a short-chain fatty acid (SCFA)-producing bacterium that is recognized to play a crucial role in maintaining intestinal homeostasis and has been suggested for application among the next-generation probiotics (NGP) 34,36 .”
Chernov, V. M., Chernova, O. A., Markelova, M., & Trushin, M. V. (2024) · Nanobiotechnology Reports · doi:10.1134/s2635167624600792 · Cited by 3
Rajini, P. S., Bharath, M. M. S., & Muralidhara, M. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-821039-0.00020-4 · Cited by 4
Yamakawa, M., Wada, K., Hayashi, M., Ezaki, T., Nakashima, Y., Nagata, C., & Sumoto, Y. (2023) · Nutrition · doi:10.1016/j.nut.2023.112145 · Cited by 3
Pető, Á., Kósa, D., Szilvássy, Z., Fehér, P. S., Ujhelyi, Z., Kovács, G. E., Német, I., Pócsi, I., & Bácskay, I. (2023) · Fermentation · doi:10.3390/fermentation9080767 · Cited by 3
“Unfortunately, their extreme sensitivity to oxygen and acidic gastric conditions make them challenging to work with [21].”
BackgroundPrebiotics and Probiotics Food
Sankarganesh, P., Adeyeye, S. A. O., Chakka, A. K., & Babu, A. S. (2024) · Wiley eBooks · doi:10.1002/9781394237623.ch16 · Cited by 4
Lobzin, Y., Gorelov, A. V., Gritsenko, V. A., Migacheva, N., Usenko, D., & Ermolenko, K. (2025) · Journal Infectology · doi:10.22625/2072-6732-2025-17-1-5-14 · Cited by 2
Koh, E. J. X., Lim, S. J., Hu, W. S. L., & Sofian‐Seng, N. (2026) · Food Bioscience · doi:10.1016/j.fbio.2026.109233 · Cited by 2
Current Status and Prospects for Standards, Regulations, and Detection of Probiotic Yogurt: Review
Chon, J., Seo, K., Kim, T.-J., Youn, H., Kang, S.-H., Hwang, W.-U., Jeong, H., Jeong, D., & Song, K. (2023) · Journal of Dairy Science and Biotechnology · doi:10.22424/jdsb.2023.41.1.9 · Cited by 2
KAMBER, A., Albayrak, Ç. B., & Harsa, Ş. (2024) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-024-10357-6 · Cited by 2
“Over the past 15 years, research has focused on the roles that A. mucin-iphila , a member of the next generation of probiotics (NGP) class [24–26], plays in the gastrointestinal system [20, 23, 27, 28].”
REMOVED: Biotechnological approaches in fish gut microbiome
Prakash, K., Vijayan, M., Venkatesan, B., Annamalai, K. K., Manigundan, K., Radhakrishnan, M., & Gopikrishnan, V. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-22348-8.00022-2 · Cited by 2
Callaway, T. R., Perez, H. G., Corcionivoschi, N., Bu, D., & Fluharty, F. L. (2024) · Journal of Dairy Science · doi:10.3168/jds.2024-25847 · Cited by 3
Integrative multiomics: Understanding postbiotics and their role in human physiology
Barathikannan, K., Chelliah, R., David, H. A., Kulandairaj, E. P., Vijayalakshmi, S., Rubab, M., & Oh, D.-H. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-22188-0.00021-8 · Cited by 2
Metadata
- Authors
- Saarela, M. H.
- Affiliations
- VTT Technical Research Centre of Finland (FI)
- Corresponding author
- Saarela, Maria H
- ORCID
- Saarela, Maria H
- Venue
- Current Opinion in Food Science
- Publisher
- Elsevier BV
- Published
- 2019-12
- Volume
- 30
- Pages
- 8-13
- ISSN
- 2214-7993 (ISSN-L)
- OpenAlex
- W2889616411
- Cited by
- 118
- References
- 51
- Citations by year
- 2026: 10 · 2025: 13 · 2024: 18 · 2023: 18 · 2022: 22 · 2021: 19 · 2020: 11 · 2019: 7
- FWCI
- 0.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Opinion in Food Science (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- next-generation probiotics, probiotic safety assessment, gut microbiota, Bacteroides, Clostridium, Faecalibacterium, Akkermansia, antibiotic resistance genes, probiotic supplements, health-promoting properties
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