Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery
Abstract
Crosslinking of polymeric network using nanoparticles by physical or chemical method to obtain hydrogel is an emerging approach. Herein, we synthesized Polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture. Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry. Developed HA-Cys conjugate was cross-linked using PDA nanoparticles via Michael-type addition reaction. Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology. FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles. Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability. Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel. Hydrogel microenvironment facilitated enhanced endothelial cell migration, proliferation and attachment. Furthermore, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro. Overall, these results demonstrate that PDA cross-linked thiol-functionalized hydrogel was developed in a facile manner under physiological conditions. These developed hydrogels could be potentially used in tissue engineering and drug deliv…
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HA-Cys/PDA hydrogel can release DMOG sustainably for 7 days.
“This injectable composite hydrogel can release dimethyloxalylglycine (DMOG) drugs sustainably for a period of 7 days.”
From Mussel-Inspired Injectable Adhesive Hydrogels for Biomedical Applications (2024) · cites this paper as [149]
HUVECs treated with DMOG-loaded HA-Cys/PDA hydrogel showed enhanced capillary tube formation.
“In vitro results indicate that human umbilical vein endothelial cells (HUVECs) treated with DMOG-loaded HA-Cys/PDA hydrogel showed an enhanced capillary tube formation, confirming its potential in tissue engineering [149].”
From Mussel-Inspired Injectable Adhesive Hydrogels for Biomedical Applications (2024) · cites this paper as [149]
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- Authors
- Yegappan, R., Selvaprithiviraj, V., Mohandas, A., & Jayakumar, R.
- Affiliations
- Amrita Institute of Medical Sciences and Research Centre (IN)
- Corresponding author
- Jayakumar, Rangasamy
- Venue
- Colloids and Surfaces B: Biointerfaces
- Publisher
- Elsevier BV
- Published
- 2019-05
- Volume
- 177
- Pages
- 41-49
- ISSN
- 0927-7765 (ISSN-L)
- OpenAlex
- W2914279126
- Cited by
- 84
- References
- 40
- Citations by year
- 2026: 10 · 2025: 11 · 2024: 16 · 2023: 9 · 2022: 15 · 2021: 12 · 2020: 8 · 2019: 3
- FWCI
- 3.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Colloids and Surfaces B Biointerfaces (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Nanomission, Department of Science and Technology (DST), Government of India
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- polydopamine nanoparticles, hydrogel crosslinking, Michael addition, dimethyloxalylglycine, sustained drug release, endothelial cell migration, capillary tube formation, injectable hydrogel, tissue engineering
- MeSH terms
- Amino Acids, Dicarboxylic, Cell Movement, Cells, Cultured, Cross-Linking Reagents, Humans, Hyaluronic Acid, Indoles, Particle Size, Polymers, Sulfhydryl Compounds, Molecular Structure, Drug Delivery Systems, Hydrogel, Polyethylene Glycol Dimethacrylate, Cell Proliferation, Nanoparticles, Human Umbilical Vein Endothelial Cells, Drug Liberation
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