The DOI matches its Crossref record; 29 of the 30 references it cites were found in a registry or an index.
Mammalian Scribble Forms a Tight Complex with the βPIX Exchange Factor
Open accessAbstract
Drosophila Scribble is implicated in the development of normal synapse structure and epithelial tissues, but it remains unclear how it plays a role and which process it controls. The mammalian homolog of Scribble, hScrib, has a primary structure and subcellular localization similar to that of its fly homolog, but its function remains unknown. Here we have used tandem mass spectrometry to identify major components of the hScrib network. We show that it includes betaPIX (also called Cool-1), a guanine nucleotide exchange factor (GEF), and its partner GIT1 (also called p95-APP1), a GTPase activating protein (GAP). betaPIX directly binds to the hScrib PDZ domains, and the hScrib/betaPIX complex is efficiently recovered in epithelial and neuronal cells and tissues. In cerebellar granule cell cultures, hScrib and betaPIX are both partially localized at neuronal presynaptic compartments. Furthermore, we show that hScrib is required to anchor betaPIX at the cell cortex and that dominant-negative betaPIX or hScrib proteins can each inhibit Ca2+-dependent exocytosis in neuroendocrine PC12 cells, demonstrating a functional relationship between these proteins. These data reveal the existence of a tight hScrib/betaPIX interaction and suggest that this complex potentially plays a role in neuronal transmission.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“A specific point mutation within LRR13 (Prol 305 to Leu, P305L) of the human SCRIB protein (hSCRIB) has also been shown to abrogate membrane localization (13), disrupting recruitment of βPIX, and exocytosis.”
From Mislocalization of the Cell Polarity Protein Scribble Promotes Mammary Tumorigenesis and Is Associated with Basal Breast Cancer (2014) · cites this paper as [13]
In the citing paper
“Scribble is an integral component of synaptic protein complexes and can coimmunoprecipitate with β-catenin, the ADP-ribosylation factor-1 GTPase-activating protein (GAP) GIT1, the Rac/Cdc42 guanine nucleotide exchange factor β-PIX, and transmembrane receptor Neurexin (Audebert et al., 2004; Sun et al., 2009; Sun and Bamji, 2011; Rui et al., 2017).”
From Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation (2019) · cites this paper as Audebert et al., 2004
Citations
Cites
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
Shevchenko, A., Wilm, M. S., Vorm, O., & Mann, M. (1996) · Analytical Chemistry · doi:10.1021/ac950914h · Cited by 8,996
Cooperative Regulation of Cell Polarity and Growth by Drosophila Tumor Suppressors
Bilder, D. H., Li, M., & Perrimon, N. (2000) · Science · doi:10.1126/science.289.5476.113 · Cited by 935
PAK Kinases Are Directly Coupled to the PIX Family of Nucleotide Exchange Factors
Manser, E. J., Loo, T.-H., Koh, C.-G., Zhao, Z.-S., Chen, X., Tan, L. H.-P., Tan, I., Leung, T., & Lim, L. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80019-2 · Cited by 753
Localization of apical epithelial determinants by the basolateral PDZ protein Scribble
Bilder, D., & Perrimon, N. (2000) · Nature · doi:10.1038/35001108 · Cited by 716
Adaptation of core mechanisms to generate cell polarity
Nelson, W. J. (2003) · Nature · doi:10.1038/nature01602 · Cited by 716
Identification of Vangl2 and Scrb1 as planar polarity genes in mammals
Montcouquiol, M., Rachel, R. A., Lanford, P. J., Copeland, N. G., Jenkins, N. A., & Kelley, M. W. (2003) · Nature · doi:10.1038/nature01618 · Cited by 705
PDZ Domain Proteins: Plug and Play!
Nourry, C., Grant, S. G. N., & Borg, J. (2003) · Science Signaling · doi:10.1126/stke.2003.179.re7 · Cited by 510
Self-citationNakagawa, S., & Huibregtse, J. M. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.21.8244-8253.2000 · Cited by 477
Coupling of PAK-Interacting Exchange Factor PIX to GIT1 Promotes Focal Complex Disassembly
Zhao, Z.-S., Manser, E. J., Loo, T.-H., & Lim, L. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.17.6354-6363.2000 · Cited by 364
A Novel Regulator of p21-activated Kinases
Bagrodia, S., Taylor, S. J., Jordon, K. A., Aelst, L. V., & Cerione, R. A. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.37.23633 · Cited by 311
Cited by
Sorted by citations
Coordinated protein sorting, targeting and distribution in polarized cells
Mellman, I., & Nelson, W. J. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2525 · Cited by 521
Zhan, L., Rosenberg, A., Bergami, K. C., Yu, M., Xuan, Z., Jaffe, A. B., Allred, C., & Muthuswamy, S. K. (2008) · Cell · doi:10.1016/j.cell.2008.09.045 · Cited by 436
Control of tumourigenesis by the Scribble/Dlg/Lgl polarity module
Humbert, P. O., Grzeschik, N. A., Brumby, A. M., Galea, R. C., Elsum, I. A., & Richardson, H. E. (2008) · Oncogene · doi:10.1038/onc.2008.341 · Cited by 323
Qin, Y., Capaldo, C., Gumbiner, B. M., & Macara, I. G. (2005) · The Journal of Cell Biology · doi:10.1083/jcb.200506094 · Cited by 285
Remodeling Epithelial Cell Organization: Transitions Between Front-Rear and Apical-Basal PolarityPreprint
Nelson, W. J. (2009) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a000513 · Cited by 286
Metadata
- Authors
- Audebert, S., Navarro, C., Nourry, C., Chasserot-Golaz, S., Lécine, P., Bellaiche, Y., Dupont, J.-L., Premont, R. T., Sempéré, C., Strub, J.-M., Van Dorsselaer, A., Vitale, N., & Borg, J.-P.
- Affiliations
- Inserm (FR)
- Centre National de la Recherche Scientifique (FR)
- Duke Medical Center (US)
- Corresponding author
- Borg, Jean-Paul
- ORCID
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2004-06
- Volume
- 14
- Issue
- 11
- Pages
- 987-995
- ISSN
- 0960-9822 (ISSN-L)
- OpenAlex
- W2007101096
- Cited by
- 218
- References
- 30
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 10 · 2020: 9 · 2019: 10 · 2018: 11 · 2017: 10 · 2016: 12 · 2015: 9 · 2014: 15 · 2013: 11 · 2012: 14
- FWCI
- 5.22
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 3333 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- Scribble, betaPIX, GIT1, PDZ domain, guanine nucleotide exchange factor, GTPase-activating protein, protein-protein interaction, presynaptic complex, exocytosis, neural transmission, cell cortex, cerebellar granule cells, synaptic structure, epithelium
- MeSH terms
- Cell Membrane, Cells, Cultured, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Exocytosis, Humans, Membrane Proteins, Phosphoproteins, Precipitin Tests, Mass Spectrometry, Presynaptic Terminals, DNA, Complementary, Cell Cycle Proteins, Reverse Transcriptase Polymerase Chain Reaction, Guanine Nucleotide Exchange Factors, GTPase-Activating Proteins, Two-Hybrid System Techniques, Tumor Suppressor Proteins, Adaptor Proteins, Signal Transducing, Rho Guanine Nucleotide Exchange Factors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
