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Single-Cell Resolution of Temporal Gene Expression during Heart Development
Open accessAbstract
Activation of complex molecular programs in specific cell lineages governs mammalian heart development, from a primordial linear tube to a four-chamber organ. To characterize lineage-specific, temporal-spatial developmental programs, we performed single-cell RNA sequencing of >1200 murine cells isolated at seven time points spanning E9.5 (primordial heart tube) to P21 (mature heart). Using unbiased transcriptional data we classified cardiomyocytes (CM), endothelial cells (EC), and fibroblast-enriched cells, thus identifying markers for temporal and chamber-specific developmental programs. Harnessing these datasets, we defined developmental ages of human and mouse pluripotent stem cell-derived CMs and characterized lineage-specific maturation defects in hearts of mice with heterozygous mutations in Nkx2.5 that cause human heart malformations. This spatial-temporal transcriptome analysis of heart development reveals lineage-specific gene programs underlying normal cardiac development and congenital heart disease.
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Metadata
- Authors
- DeLaughter, D. M., Bick, A. G., Wakimoto, H., McKean, D., Gorham, J. M., Kathiriya, I. S., Hinson, J. T., Homsy, J., Gray, J., Pu, W., Bruneau, B. G., Seidman, J. G., & Seidman, C. E.
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 39
- Issue
- 4
- Pages
- 480-490
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2556548281
- Cited by
- 423
- References
- 54
- FWCI
- 18.96
- Type
- journal article
- Language
- en
- Open access
- bronze
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