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Cited by 16Fatty Acid Research and HealthInflammatory mediators and NSAID effectsAsthma and respiratory diseases

The DPA-derivative 11S, 17S-dihydroxy 7,9,13,15,19 (Z,E,Z,E,Z)-docosapentaenoic acid inhibits IL-6 production by inhibiting ROS production and ERK/NF-κB pathway in keratinocytes HaCaT stimulated with a fine dust PM10

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Choi, Myoung-Kwon, Kim, Jinju, Park, Hyo-Min, Lim, Chae-Min, Pham, Thu-Huyen, Shin, Ha Youn, Kim, Seong-Eun, Oh, Deok-Kun +1 more·2022·Ecotoxicology and Environmental Safety
DOI 10.1016/j.ecoenv.2022.113252
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Abstract

11 S, 17S-dihydroxy 7,9,13,15,19 (Z,E,Z,E,Z)-docosapentaenoic acid (DoPE) is a derivative of docosapentaenoic acid, a specialized pro-resolving mediator of inflammation such as lipoxins, resolvins, maresins, and protectins. PM10 is a fine dust particle that induces oxidative stress, DNA damage, inflammation, aging, and cancer. The anti-inflammatory mechanism of DoPE, however, has not yet been elucidated. In these studies, we investigated whether DoPE has anti-inflammatory effects in human keratinocyte HaCaT cells. We demonstrated that DoPE suppressed PM10-induced expressions of IL-6 mRNA and protein in human HaCaT keratinocytes. We also investigated the modulating effects of DoPE on reactive oxygen species (ROS) and mitogen-activated protein kinase (MAPK). ROS production, extracellular signal regulated kinase (ERK) phosphorylation, and translocation of nuclear factor-kappa B (NF-kB) p65 and NF-kB activity were suppressed by DoPE in PM10-stimulated HaCaT cells. Collectively, our results demonstrated that DoPE inhibited IL-6 expression by reducing ROS generation, suppressing ERK phosphorylation, and inhibiting translocation of NF-kB p65 and NF-kB activity in PM10-stimulated HaCaT cells, suggesting that DoPE can be useful for the resolution of the inflammation caused by IL-6.

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  • Docosapentaenoic acid (DPA), a key component of PS (18:0/22:5)-H, demonstrates potent anti-inflammatory effects, enhancing macrophage polarization and reducing ROS production.

    “Docosapentaenoic acid (DPA), a key component of PS (18:0/22:5)‐H, demonstrates potent anti‐inflammatory effects, enhancing macrophage polarization and reducing ROS production (Choi et al. 2022; Ma et al. 2020).”

    From Transcript and Lipid Profile Alterations in Astrocyte‐Neuron Mitochondrial Transfer Under Lipopolysaccharide Exposure: An In Vitro Study (2025) · cites this paper as Choi et al. 2022

    The Introduction identifies DoPE as a DPA-derived compound, and the Conclusion reports that DoPE reduced ROS production and modulated IL-6 in PM10-stimulated HaCaT keratinocytes. The paper does not establish those effects for DPA itself, identify DPA as a component of PS (18:0/22:5)-H, or report enhanced macrophage polarization. The full-text copy supplies no page numbers for these sections. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • PS (18:0/22:5)-H and PS (18:0/18:1)-H have known anti-inflammatory properties.

    “which have known anti‐inflammatory properties.”

    From Transcript and Lipid Profile Alterations in Astrocyte‐Neuron Mitochondrial Transfer Under Lipopolysaccharide Exposure: An In Vitro Study (2025) · cites this paper as Choi et al. 2022

    The Introduction and Results section 3.2 discuss the anti-inflammatory effects of DoPE, a docosapentaenoic-acid derivative, in PM10-stimulated keratinocytes. Searches for PS (18:0/22:5)-H, PS (18:0/18:1)-H, and phosphatidylserine found no passage addressing the anti-inflammatory properties of either claimed compound. Page numbers were not available in our copy. (LLM, conf=0.76)

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Cited by

Sorted by citations

Authors
Choi, M.-K., Kim, J., Park, H.-M., Lim, C.-M., Pham, T.-H., Shin, H. Y., Kim, S.-E., Oh, D.-K., & Yoon, D.-Y.
Affiliations
Konkuk University (KR)
Corresponding author
Yoon, Do-Young
ORCID
Venue
Ecotoxicology and Environmental Safety
Publisher
Elsevier BV
Published
2022-03
Volume
232
Pages
113252
ISSN
0147-6513 (ISSN-L)
DOI
10.1016/j.ecoenv.2022.113252
OpenAlex
W4210382541
Cited by
16
References
2
Citations by year
2026: 1 · 2025: 2 · 2024: 4 · 2023: 6 · 2022: 3
FWCI
2.20
Type
journal article
Language
en
Open access
Licence
Locations
  • Ecotoxicology and Environmental Safety (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
National Research Foundation of Korea (2020R1A4A1018648, 2021R1A2C 3014577)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
  • Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
Keywords
specialized pro-resolving mediators, PM10, particulate matter, interleukin-6, reactive oxygen species, ERK1/2 phosphorylation, p65 nuclear translocation, MAPK signaling, keratinocytes, HaCaT keratinocytes, anti-inflammatory activity, inflammation resolution, oxidative stress
MeSH terms
Dust, Fatty Acids, Unsaturated, Humans, Keratinocytes, Interleukin-6, NF-kappa B, Reactive Oxygen Species, Extracellular Signal-Regulated MAP Kinases

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