Effect of age and CMV on NK cell subpopulations
Abstract
NK cells represent an important component of the innate immune response against infection and tumors. Age-associated changes in NK cell phenotype have been previously reported that can be responsible of functional NK cell deficiency. The aim of this work was to analyze the effect CMV seropositivity and aging on the distribution of NK cell subsets with a focus on the expression of cytotoxicity-related molecules and on the expression of CD94/NKG2 heterodimers and CD57 on these NK cell subsets. Our results show that CMV seropositivity in young individuals does not significantly affect peripheral blood NK cell percentage and NK cell subsets defined by the use of CD56 and CD16 markers. In contrast a significant increase in the percentage of NK cells is observed in elderly donors, all of them are CMV seropositive, when compared with young CMV seropositive subjects. A decrease in the percentage of CD56bright NK cells, either fully immature CD16 negative or CD16+ and an increase in the CD56-CD16+ subset are also found in the elderly. CMV seropositivity either in healthy young or elderly individuals is associated to the expression of CD94/NKG2C dimers and high expression of CD57on the CD56dimCD16+ NK cell subset. CD56-CD16+ NK cells, which are expanded in the elderly, show a decreased expression of granzymes A and B and an increased expression of CD94/NKG2C and CD57 in CMV seropositive young donors when compared with CMV seronegative young individuals. These results indicate that CMV and age have a different effect on NK cell phenotype and emphasize the relevance of including the determination of CMV serostatus in those studies addressed to analyze the immune response in the elderly.
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Changes in the expression of these activated receptors in the elderly may affect the immune monitoring function of NK cells.
“Therefore, changes in the expression of these activated receptors in the elderly may affect the immune monitoring function of NK cells [85, 86].”
From Immunosenescence: a key player in cancer development (2020) · cites this paper as [86]
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- Authors
- Campos, C., Pera, A., Sanchez-Correa, B., Alonso, C., Lopez-Fernandez, I., Morgado, S., Tarazona, R., & Solana, R.
- Affiliations
- Instituto Maimónides de Investigación Biomédica de Córdoba (ES)
- Universidad de Extremadura (ES)
- Corresponding author
- Pera, Alejandra
- Venue
- Experimental Gerontology
- Publisher
- Elsevier BV
- Published
- 2014-06
- Volume
- 54
- Pages
- 130-137
- ISSN
- 0531-5565 (ISSN-L)
- OpenAlex
- W1975576166
- Cited by
- 128
- References
- 50
- Citations by year
- 2026: 7 · 2025: 13 · 2024: 7 · 2023: 12 · 2022: 11 · 2021: 8 · 2020: 9 · 2019: 13 · 2018: 8 · 2017: 12 · 2016: 11 · 2015: 9 · 2014: 8
- FWCI
- 6.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Gerontology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Spanish Ministry of Health (PS09/00723)
- Ministry of Science and Innovation of Spain (SAF2009-09711)
- Junta de Andalucia (PRI09A029)
- Junta de Extremadura (GRU10104)
- University of Extremadura
- European Regional Development Fund (FEDER)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Cell Function and Interaction
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Keywords
- natural killer cells, cytomegalovirus seropositivity, aging, NK cell subsets, CD56, CD16, CD94/NKG2C, CD57, NK cell cytotoxicity, NK cell deficiency, elderly donors
- MeSH terms
- Adult, Aged, Aged, 80 and over, Cytomegalovirus Infections, Female, Humans, Killer Cells, Natural, Male, Lymphocyte Subsets, CD57 Antigens, CD56 Antigen, NK Cell Lectin-Like Receptor Subfamily D, Granzymes, Perforin, Young Adult
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