Mitochondrial dynamics signaling is shifted toward fusion in muscles of very old hip-fractured patients: results from the Sarcopenia in HIp FracTure (SHIFT) exploratory study
Abstract
Background Mitochondrial quality control (MQC) is crucial for maintaining mitochondrial fitness. We investigated MQC signaling in muscle of old hip-fractured patients.Methods Twenty-three patients, enrolled in the Sarcopenia in HIp FracTure (SHIFT) study, were categorized into old (OL; n=8) and very old groups (VOL; n=15) using 85years as the cut-off. The expression of a set of MQC signaling proteins was assayed in vastus lateralis muscle biopsies.Results The content of lysosome-associated membrane protein 2, microtubule-associated protein 1 light chain 3B, optic atrophy protein 1, fission protein 1 (Fis1), peroxisome proliferator-activated receptor-γ coactivator-1α, and forkhead box O3 was unvaried between groups. Conversely, the protein expression of mitofusin 2 (Mfn2) as well as the fusion index (Mfn2/Fis1) was increased in VOL patients.Conclusions Muscle mitochondrial dynamics appear to be shifted toward fusion in very advanced age. Whether this phenomenon represents an adaptation to cope with age-dependent mitochondrial dysfunction warrants further investigation.
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The higher content of Mfn2 is in line with morphological data and previous studies that have suggested an increase in mitochondrial fusion in aged muscle.
“While the higher content of Mfn2 is in line with our morphological data and previous studies that have suggested an increase in mitochondrial fusion in aged muscle (Leduc-Gaudet et al., 2015; Picca et al., 2017), the concomitant increase in Drp1 and Fis1 also suggests enhanced mitochondrial fission in the WG of aged rats.”
From Effects of Aging and Caloric Restriction on Fiber Type Composition, Mitochondrial Morphology and Dynamics in Rat Oxidative and Glycolytic Muscles (2019) · cites this paper as Picca et al., 2017
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- Authors
- Picca, A., Calvani, R., Lorenzi, M., Menghi, A., Galli, M., Vitiello, R., Randisi, F., Bernabei, R., Landi, F., & Marzetti, E.
- Affiliations
- Università Cattolica del Sacro Cuore (IT)
- Corresponding author
- Calvani, Riccardo
- ORCID
- Venue
- Experimental Gerontology
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 96
- Pages
- 63-67
- ISSN
- 0531-5565 (ISSN-L)
- OpenAlex
- W2624469129
- Cited by
- 32
- References
- 21
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 4 · 2020: 9 · 2019: 1 · 2018: 4 · 2017: 2 · 2012: 1
- FWCI
- 1.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Gerontology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fondazione Roma
- Innovative Medicine Initiative-Joint Undertaking (115621)
- Intramural Research Grants from the Catholic University of the Sacred Heart (D3.2 2013, D3.2 2015)
- Centro Studi Achille e Linda Lorenzon
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- mitochondrial dynamics, mitochondrial fusion, mitochondrial quality control, oldest old, hip fracture, sarcopenia, skeletal muscle, mitofusin 2, Fis1, mitochondrial fission, mitochondrial dysfunction, vastus lateralis, protein expression
- MeSH terms
- Forkhead Box Protein O3, Age Factors, Aged, 80 and over, Female, Hip Fractures, Humans, Male, Microtubule-Associated Proteins, Mitochondria, Muscle, Muscle Proteins, Signal Transduction, Lysosomal-Associated Membrane Protein 2, Sarcopenia, Mitochondrial Dynamics
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