Flavan 3-ol Delays the Progression of Disuse Atrophy Induced by Hindlimb Suspension in Mice
Abstract
Periods of skeletal muscle disuse, for example due to a sedentary lifestyle or bed rest, are associated with aging and can lead to muscle atrophy. We previously found that the flavan 3-ol fraction derived from cocoa (FL) enhanced energy expenditure with metabolic changes in skeletal muscle. In the present study, we examined the effect of FL on disuse muscle atrophy induced by hindlimb suspension in mice. Male C57BL/6J mice were assigned to four groups as follows: unsuspended-vehicle, unsuspended-FL, suspended-vehicle, and suspended-FL. Mice in the vehicle treatment groups were administered distilled water and those in the FL treatment groups were dosed with FL (50mg/kg/day) for 2weeks. The weights of the gastrocnemius (GC), tibialis anterior (TA), and soleus (SOL), but not the extensor digitorum longus (EDL), decreased significantly in mice with hindlimb suspension (-11.8%, -16.5%, and -41.0%, respectively). This reduction in GC, TA, and SOL mass was inhibited by FL (-5.3%, +2.0%, and -16.6%, respectively). The FL increased the EDL weight >20% with or without hindlimb suspension. The protein level of the ubiquitin ligase, muscle ring finger-1, in the SOL was significantly increased by hindlimb suspension, but inhibited by treatment with FL. Protein expression of p70S6 kinase in the SOL was significantly decreased by hindlimb suspension, and FL treatment inhibited this change. These results suggested that FL delayed disuse muscle atrophy by metabolic alteration.
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Flavan 3-ol fraction derived from cocoa enhances muscle atrophy induced by hindlimb suspension in C57BL/6J mice.
“Flavan 3-ol fraction, derived from cocoa, enhances muscle atrophy induced by hindlimb suspension in C57BL/6J mice [98].”
From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [98]
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- Authors
- Ito, M., Kudo, N., Miyake, Y., Imai, T., Unno, T., Yamashita, Y., Hirota, Y., Ashida, H., & Osakabe, N.
- Affiliations
- Shibaura Institute of Technology (JP)
- Tokyo Kasei Gakuin University (JP)
- Kobe University (JP)
- Corresponding author
- Osakabe, Naomi
- Venue
- Experimental Gerontology
- Publisher
- Elsevier BV
- Published
- 2017-11
- Volume
- 98
- Pages
- 120-123
- ISSN
- 0531-5565 (ISSN-L)
- OpenAlex
- W2750574847
- Cited by
- 10
- References
- 29
- Citations by year
- 2024: 1 · 2023: 3 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 2
- FWCI
- 0.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Gerontology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (17H00818, 16K18924)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Keywords
- flavan-3-ols, cocoa flavanols, disuse atrophy, hindlimb unloading, skeletal muscle, muscle mass, MuRF1, ubiquitin ligase, p70 S6 kinase, muscle protein degradation, muscle metabolism, gastrocnemius, tibialis anterior, soleus muscle, extensor digitorum longus
- MeSH terms
- Tripartite Motif Proteins, Animals, Disease Models, Animal, Energy Metabolism, Flavonoids, Male, Mice, Inbred C57BL, Muscle Proteins, Muscular Atrophy, Time Factors, Muscle, Skeletal, Muscle Fibers, Fast-Twitch, Hindlimb Suspension, Ribosomal Protein S6 Kinases, 70-kDa, Ubiquitin-Protein Ligases
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