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Deep sequencing and proteomic analysis of the microRNA-induced silencing complex in human red blood cells.

Azzouzi, Imane, Moest, Hansjoerg, Wollscheid, Bernd, Schmugge, Markus, Eekels, Julia J.M., Speer, Oliver·2015·Experimental Hematology
DOI 10.1016/j.exphem.2015.01.007
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Abstract

During maturation, erythropoietic cells extrude their nuclei but retain their ability to respond to oxidant stress by tightly regulating protein translation. Several studies have reported microRNA-mediated regulation of translation during terminal stages of erythropoiesis, even after enucleation. In the present study, we performed a detailed examination of the endogenous microRNA machinery in human red blood cells using a combination of deep sequencing analysis of microRNAs and proteomic analysis of the microRNA-induced silencing complex. Among the 197 different microRNAs detected, miR-451a was the most abundant, representing more than 60% of all read sequences. In addition, miR-451a and its known target, 14-3-3ζ mRNA, were bound to the microRNA-induced silencing complex, implying their direct interaction in red blood cells. The proteomic characterization of endogenous Argonaute 2-associated microRNA-induced silencing complex revealed 26 cofactor candidates. Among these cofactors, we identified several RNA-binding proteins, as well as motor proteins and vesicular trafficking proteins. Our results demonstrate that red blood cells contain complex microRNA machinery, which might enable immature red blood cells to control protein translation independent of de novo nuclei information.

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Metadata

Authors
Azzouzi, I., Moest, H., Wollscheid, B., Schmugge, M., Eekels, J. J. M., & Speer, O.
Venue
Experimental Hematology
Publisher
Elsevier BV
Published
2015-05
Volume
43
Issue
5
Pages
382-392
ISSN
0301-472X
DOI
10.1016/j.exphem.2015.01.007
Cited by
39
References
2
Language
en
Licence
Funders
Roche (5002248765)

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