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Cited by 114Essential Oils and Antimicrobial ActivityNatural Compounds in Disease TreatmentSesquiterpenes and Asteraceae Studies

Artemisia fukudo essential oil attenuates LPS-induced inflammation by suppressing NF-κB and MAPK activation in RAW 264.7 macrophages

Yoon, W.J., Moon, J.Y., Song, G., Lee, Y.K., Han, M.S., Lee, J.S., Ihm, B.S., Lee, W.J. +2 more·2010·Food and Chemical Toxicology
DOI 10.1016/j.fct.2010.02.014
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Abstract

In the present study, the chemical constituents of Artemisia fukudo essential oil (AFE) were investigated using GC-MS. The major constituents were alpha-thujone (48.28%), beta-thujone (12.69%), camphor (6.95%) and caryophyllene (6.01%). We also examined the effects of AFE on the production of nitric oxide (NO), prostaglandin E(2) (PGE(2)), tumour necrosis factor (TNF)-alpha, interleukin (IL)-1beta, and IL-6, in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. Western blotting and RT-PCR tests indicated that AFE has potent dose-dependent inhibitory effects on pro-inflammatory cytokines and mediators. We investigated the mechanism by which AFE inhibits NO and PGE(2) by examining the level of nuclear factor-kappaB (NF-kappaB) activation within the mitogen-activated protein kinase (MAPK) pathway, which is an inflammation-induced signal pathway in RAW 264.7 cells. AFE inhibited LPS-induced ERK, JNK, and p38 phosphorylation. Furthermore, AFE inhibited the LPS-induced phosphorylation and degradation of Ikappa-B-alpha, which is required for the nuclear translocations of the p50 and p65 NF-kappaB subunits in RAW 264.7 cells. Our results suggest that AFE might exert an anti-inflammatory effect by inhibiting the expression of pro-inflammatory cytokines. Such an effect is mediated by a blocking of NF-kappaB activation which consequently inhibits the generation of inflammatory mediators in RAW264.7 cells. AFE may be useful for treating inflammatory diseases.

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  • Artemisia fukudo oil main components inhibit release of TNF-α, IL-1β, and IL-6 in LPS-treated RAW 264.7 cells.

    “α-Thujone, β-thujone, camphor, and caryophyllene were the main components of Artemisia fukudo oil that were able to inhibit the release of TNF-α, IL-1β, and IL-6 in LPS-treated RAW 264.7 cells [116].”

    From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [116]

  • iNOS catalyses production of large amounts of NO from L-arginine and molecular oxygen and is mainly triggered and regulated by signalling pathways including NF-κB and MAPKs.

    “iNOS catalises the production of large amount of NO from L-arginine and molecular oxygen, is mainly triggered and regulated by a series of signalling pathways including nuclear factor-κB (NF-κB) transcription factor and mitogen-activated protein kinases (MAPKs) [116].”

    From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [116]

  • The inhibitory effect of some essential oils on pro-inflammatory cytokine production seems to be mediated by suppressing gene expression of these cytokines, mainly at the transcriptional level.

    “The inhibitory effect of some of the essential oils on the production of pro-inflammatory cytokines reported in this chapter seems to be mediated by suppressing gene expression of these cytokines. Such was described by some authors because they found that the essential oils studied significantly suppressed the protein and mRNA expression of the cytokines in stimulated cells, assuming therefore,”

    From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [116]

  • Artemisia fukudo essential oil attenuates LPS-induced inflammation by suppressing NF-κB and MAPK activation in RAW 264.7 macrophages, acting through reduction of IκBα phosphorylation and blockage of NF-κB and translocation of p50 and p65 units to the nucleus, and suppresses LPS-induced phosphorylation of ERK, JNK, and p38 MAPKs.

    “Artemisia fukudo Makino essential oil, mainly constituted by α-thujone, β-thujone, camphor, and caryophyllene attenuates LPS-induced inflammation by suppressing NF-κB and MAPK activation in RAW 264.7 macrophages. The essential oil in LPS-induced inflammation act through the reduction of IκBα phosphorilation and blockage of NF-κB and translocation of p50 and p65 units to the nucleus. The same oil”

    From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [116]

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Authors
Yoon, W. J., Moon, J. Y., Song, G., Lee, Y. K., Han, M. S., Lee, J. S., Ihm, B. S., Lee, W. J., Lee, N. H., & Hyun, C. G.
Affiliations
  • Mokpo National University (KR)
  • Jeju National University (KR)
Corresponding author
Hyun, C.G.
ORCID
Venue
Food and Chemical Toxicology
Publisher
Elsevier BV
Published
2010-05
Volume
48
Issue
5
Pages
1222-1229
ISSN
0278-6915 (ISSN-L)
DOI
10.1016/j.fct.2010.02.014
OpenAlex
W2048551183
Cited by
114
References
35
Citations by year
2026: 7 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 16 · 2021: 5 · 2020: 4 · 2019: 8 · 2018: 6 · 2017: 8 · 2016: 9 · 2015: 9 · 2014: 7 · 2013: 8 · 2012: 7
FWCI
11.27
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Food and Chemical Toxicology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Sesquiterpenes and Asteraceae Studies
Keywords
essential oils, anti-inflammatory activity, lipopolysaccharide-induced inflammation, RAW 264.7 macrophages, NF-κB signaling, MAPK signaling, α-thujone, β-thujone, camphor, caryophyllene, nitric oxide, prostaglandin E2, inflammatory cytokines, IκBα phosphorylation, ERK, JNK, p38 MAPK
MeSH terms
Animals, Anti-Inflammatory Agents, Non-Steroidal, Cell Line, Cell Survival, Macrophages, Gas Chromatography-Mass Spectrometry, Oils, Volatile, Plant Oils, RNA, Messenger, Down-Regulation, Gene Expression, Cytokines, NF-kappa B, Inflammation Mediators, Artemisia, Gene Expression Profiling, Mitogen-Activated Protein Kinases, Cell Proliferation, Mice

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