Cited by 116Light effects on plantsPlant Molecular Biology ResearchPhotosynthetic Processes and Mechanisms

Dimerization of the plant photoreceptor phototropin is probably mediated by the LOV1 domain

Salomon, Michael, Lempert, Ulrika, Rüdiger, Wolfhart·2004·FEBS Letters

Abstract

Phototropin is a membrane‐bound UV‐A/blue light photoreceptor of plants responsible for phototropism, chloroplast migration and stomatal opening. Characteristic are two LOV domains, each binding one flavin mononucleotide, in the N‐terminal half and having a serine/threonine kinase domain in the C‐terminal half of the molecule. We purified the N‐terminal half of oat phototropin 1, containing LOV1 and LOV2 domains, as a soluble fusion protein with the calmodulin binding peptide (CBP) by expression in Escherichia coli . Gel chromatography showed that it was dimeric in solution. While the fusion protein CBP‐LOV2 was exclusively monomeric in solution, the fusion protein CBP‐LOV1 occurred as monomer and dimer. The proportion of dimer increased on prolonged incubation. We conclude that native phototropin is a dimer and that the LOV1 domain is probably responsible for dimerization.

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Crossref · 10.1016/j.febslet.2004.06.081

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Authors
Salomon, M., Lempert, U., & Rüdiger, W.
Affiliations
Ludwig-Maximilians-Universität München (DE)
Corresponding author
Rüdiger, Wolfhart
Venue
FEBS Letters
Publisher
Wiley
Published
2004-07-14
Volume
572
Issue
1-3
Pages
8-10
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W2027250591
Cited by
116
References
13
Citations by year
2026: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 3 · 2015: 2 · 2014: 5 · 2013: 6 · 2012: 12
FWCI
16.81
Type
journal article
Language
en
Open access
closed
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
Keywords
phototropin, LOV2 domain, protein dimerization, flavin mononucleotide, photoreceptors, calmodulin-binding peptide, phototropism, soluble fusion protein
MeSH terms
Binding Sites, Biological Clocks, Cloning, Molecular, Flavoproteins, Flavin Mononucleotide, Plant Proteins, Recombinant Proteins, Polymerase Chain Reaction, Protein Serine-Threonine Kinases, Avena, Dimerization, Cryptochromes

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