Dimerization of the plant photoreceptor phototropin is probably mediated by the LOV1 domain
Abstract
Phototropin is a membrane‐bound UV‐A/blue light photoreceptor of plants responsible for phototropism, chloroplast migration and stomatal opening. Characteristic are two LOV domains, each binding one flavin mononucleotide, in the N‐terminal half and having a serine/threonine kinase domain in the C‐terminal half of the molecule. We purified the N‐terminal half of oat phototropin 1, containing LOV1 and LOV2 domains, as a soluble fusion protein with the calmodulin binding peptide (CBP) by expression in Escherichia coli . Gel chromatography showed that it was dimeric in solution. While the fusion protein CBP‐LOV2 was exclusively monomeric in solution, the fusion protein CBP‐LOV1 occurred as monomer and dimer. The proportion of dimer increased on prolonged incubation. We conclude that native phototropin is a dimer and that the LOV1 domain is probably responsible for dimerization.
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The native phototropins is reportedly a dimer, with the LOV1 domain responsible for dimerization.
“The native phototropins is reportedly a dimer, with the LOV1 domain responsible for dimerization (Salomon et al., 2004).”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Salomon et al., 2004
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- Authors
- Salomon, M., Lempert, U., & Rüdiger, W.
- Affiliations
- Ludwig-Maximilians-Universität München (DE)
- Corresponding author
- Rüdiger, Wolfhart
- ORCID
- Salomon, Michael
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2004-07-14
- Volume
- 572
- Issue
- 1-3
- Pages
- 8-10
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2027250591
- Cited by
- 116
- References
- 13
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 3 · 2015: 2 · 2014: 5 · 2013: 6 · 2012: 12
- FWCI
- 16.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- phototropin, LOV2 domain, protein dimerization, flavin mononucleotide, photoreceptors, calmodulin-binding peptide, phototropism, soluble fusion protein
- MeSH terms
- Binding Sites, Biological Clocks, Cloning, Molecular, Flavoproteins, Flavin Mononucleotide, Plant Proteins, Recombinant Proteins, Polymerase Chain Reaction, Protein Serine-Threonine Kinases, Avena, Dimerization, Cryptochromes
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