Phototropin 1 Mediates High-Intensity Blue Light-Induced Chloroplast Accumulation Response in a Root Phototropism 2-Dependent Manner in Arabidopsis phot2 Mutant Plants
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Phototropins, namely, phototropin 1 (phot1) and phototropin 2 (phot2), mediate chloroplast movement to maximize photosynthetic efficiency and prevent photodamage in plants. Phot1 primarily functions in chloroplast accumulation process, whereas phot2 mediates both chloroplast avoidance and accumulation responses. The avoidance response of phot2-mediated chloroplasts under high-intensity blue light (HBL) limited the understanding of the function of phot1 in the chloroplast accumulation process at the HBL condition. In this study, we showed that the phot2 mutant exhibits a chloroplast accumulation response under HBL, which is defective when the root phototropism 2 (RPT2) gene is mutated in the phot2 background, mimicking the phenotype of the phot1 phot2 double mutant. A further analysis revealed that the expression of RPT2 was induced by HBL and the overexpression of RPT2 could partially enhance the chloroplast accumulation response under HBL. These results confirmed that RPT2 also participates in regulating the phot1-mediated chloroplast accumulation response under HBL. In contrast, RPT2 functions redundantly with neural retina leucine zipper (NRL) protein for chloroplast movement 1 (NCH1) under low-light irradiation. In addition, no chloroplast accumulation response was detected in the phot2 jac1 double mutant under HBL, which has been previously observed in phot2 rpt2 and phot1 phot2 double mutants. Taken together, our results indicated that phot1 mediates the HBL-induced chloroplast accumulation response in an RPT2-dependent manner and is also regulated by j-domain protein required for chloroplast accumulation response 1 (JAC1).
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Arabidopsis contains two blue light photoreceptors, phot1 and phot2, which mediate chloroplast accumulation responses and are regulated by the phototropin-interacting proteins NCH1 and RPT2.
“Arabidopsis contains two blue light photoreceptors, phot1 and phot2, mediate chloroplast accumulation responses and regulate by two phototropin-interacting proteins, NCH1 and RPT2”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Wang et al., 2021
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- Authors
- Wang, J., Liang, Y.-P., Zhu, J.-D., Wang, Y.-X., Yang, M.-Y., Yan, H.-R., Lv, Q.-Y., Cheng, K., Zhao, X., & Zhang, X.
- Affiliations
- Henan University (CN)
- Corresponding author
- Zhao, Xiang
- Zhang, Xiao
- Venue
- Frontiers in Plant Science
- Publisher
- Frontiers Media SA
- Published
- 2021-09-27
- Volume
- 12
- Pages
- 704618-704618
- ISSN
- 1664-462X (ISSN-L)
- PMC
- PMC8502927
- OpenAlex
- W3203440866
- Cited by
- 15
- References
- 50
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 3 · 2023: 4 · 2022: 3
- FWCI
- 2.70
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Frontiers in Plant Science (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (31570294, 31871419, 31870272, 32070262, 2016YFD0101900)
- Henan University (21IRTSTHN019)
- Université de Lausanne
- National Key Research and Development Program of China ([2016YFD0101900])
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- phototropin 1, phototropin 2, chloroplast movement, Arabidopsis thaliana, photodamage, light signaling
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