Cited by 107Light effects on plantsPlant Molecular Biology ResearchPhotosynthetic Processes and Mechanisms

In Vivo Phosphorylation Site Mapping and Functional Characterization of Arabidopsis Phototropin 1

Open access
Sullivan, Stuart, Thomson, Catriona E., Lamont, Douglas J., Jones, Matthew A., Christie, John M.·2008·Molecular Plant

Abstract

Phototropins (phot1 and phot2) are blue-light receptor kinases controlling a range of responses that optimize the photosynthetic efficiency of plants. Light sensing is mediated by two flavin-binding motifs, known as LOV1 and LOV2, located within the N-terminal region of the protein. Photoexcitation via LOV2 leads to activation of the C-terminal kinase domain and consequently receptor autophosphorylation. However, knowledge of the in-vivo phosphorylation sites for Arabidopsis phototropins is lacking and has impeded progress in elucidating the functional significance of receptor phosphorylation. We have purified phot1 from Arabidopsis and identified the in-vivo sites of receptor phosphorylation by liquid chromatography tandem mass spectrometry. Arabidopsis-derived phot1 binds flavin mononucleotide as chromophore and is phosphorylated at four major sites located upstream of LOV2 (Ser(58), Ser(85), Ser(350), and Ser(410)), three of which are induced by blue light. Nevertheless, structure-function analysis indicates that the biological activity of phot1 can be attributed to a modular unit comprising the LOV2-kinase region of the protein. Thus, peptide regions upstream of LOV2, including the sites of receptor phosphorylation identified here, do not appear to be important for receptor signaling. By contrast, these regions may be necessary for maximizing stomatal performance and possibly light-induced relocalization of phot1.

Integrity signals

Registry

Crossref · 10.1093/mp/ssm017

Cites

65 of 65 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Sullivan, S., Thomson, C. E., Lamont, D. J., Jones, M. A., & Christie, J. M.
Affiliations
  • University of Glasgow (GB)
  • University of Dundee (GB)
Venue
Molecular Plant
Publisher
Elsevier BV
Published
2008-01
Volume
1
Issue
1
Pages
178-194
ISSN
1674-2052 (ISSN-L)
OpenAlex
W2081218825
Cited by
107
References
65
Citations by year
2026: 7 · 2025: 5 · 2024: 5 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 5 · 2017: 5 · 2016: 5 · 2015: 5 · 2014: 9 · 2013: 3 · 2012: 13
FWCI
6.46
Type
journal article
Language
en
Open access
Locations
  • Molecular Plant (publishedVersion)
  • PubMed (publishedVersion)
  • Open Access at Essex (University of Essex) (submittedVersion)
Funders
  • Royal Society
  • Biotechnology and Biological Sciences Research Council (BB/C000366/1)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
Keywords
phototropin 1, phot1, receptor autophosphorylation, kinase domain, stomatal regulation, protein relocalization, Arabidopsis thaliana
MeSH terms
Chromosome Mapping, DNA-Binding Proteins, Flavin Mononucleotide, Light, Phosphoproteins, Phosphorylation, Photosynthesis, Recombinant Fusion Proteins, Mass Spectrometry, Protein Serine-Threonine Kinases, Arabidopsis, Genes, Reporter, Arabidopsis Proteins, Green Fluorescent Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review