Impact of the unfolded protein response upon genome-wide expression patterns, and the role of Hac1 in the polarized growth, of Candida albicans
Open accessAbstract
The unfolded protein response (UPR) regulates the expression of genes involved in the protein secretory pathway and in endoplasmic reticulum (ER) stress in yeasts and filamentous fungi. We have characterized the global transcriptional response of Candida albicans to ER stresses (dithiothreitol and tunicamycin) and established the impact of the transcription factor Hac1 upon this response. Expression of C. albicans Hac1, which is the functional homologue of Saccharomyces cerevisiae Hac1p, is predicted to be translationally regulated via an atypical mRNA splicing event during ER stress. C. albicans genes involved in secretion, vesicle trafficking, stress responses and cell wall biogenesis are up-regulated in response to ER stress, and translation and ribosome biogenesis genes are down-regulated. Hac1 is not essential for C. albicans viability, but plays a major role in this stress-related transcriptional response and is required for resistance to ER stress. In addition, we show that Hac1 plays an important role in regulating the morphology of C. albicans and in the expression of genes encoding cell surface proteins during ER stress, factors that are important in virulence of this fungal pathogen.
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“Research has demonstrated that in C. albicans , 76 genes are regulated by the transcription factor Hac1 and are involved in the UPR [43].”
- Authors
- Wimalasena, T. T., Enjalbert, B., Guillemette, T., Plumridge, A., Budge, S., Yin, Z., Brown, A. J. P., & Archer, D. B.
- Affiliations
- University of Nottingham (GB)
- University of Aberdeen (GB)
- Venue
- Fungal Genetics and Biology
- Publisher
- Elsevier BV
- Published
- 2008-09
- Volume
- 45
- Issue
- 9
- Pages
- 1235-1247
- ISSN
- 1087-1845 (ISSN-L)
- OpenAlex
- W2049070379
- Cited by
- 118
- References
- 80
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 6 · 2023: 6 · 2022: 7 · 2021: 3 · 2020: 5 · 2019: 3 · 2018: 8 · 2017: 5 · 2016: 7 · 2015: 7 · 2014: 8 · 2013: 11 · 2012: 4
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- green
- hal.science
- Licence
- Locations
- Fungal Genetics and Biology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Funders
- Wellcome Trust (063204, 08088)
- Biotechnology and Biological Sciences Research Council (1/P17124, BB/C510391/1)
- British Federation of Women Graduates
- European Commission (MRTN-CT-2003-5O4148)
- University of Nottingham
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Endoplasmic Reticulum Stress and Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- unfolded protein response, HAC1, Candida albicans, endoplasmic reticulum stress, polarized growth, mRNA splicing, translational regulation, protein secretion, vesicle trafficking, cell wall biosynthesis, ribosome biogenesis, fungal virulence, cell surface proteins, tunicamycin, dithiothreitol
- MeSH terms
- Amino Acid Sequence, Base Sequence, Candida albicans, Cell Wall, Dithiothreitol, Endoplasmic Reticulum, Fungal Proteins, Molecular Sequence Data, Repressor Proteins, Transcription, Genetic, Tunicamycin, Gene Expression Regulation, Fungal, Sequence Alignment, Genome, Fungal, Cell Polarity, Oligonucleotide Array Sequence Analysis, Untranslated Regions, Gene Expression Profiling
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