Strain-specific metabolomic diversity of Lactiplantibacillus plantarum under aerobic and anaerobic conditions
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The chemotaxonomic diversity of 20 Lactiplantibacillus plantarum strains was investigated using non-targeted metabolite profiling under different culture conditions. Multivariate and metabolic pathway analyses based on GC-MS and LC-MS/MS datasets showed that amino acid metabolism, especially 2-hydroxy acids, was enriched under aerobic conditions (AE), whereas fatty acid & sugar metabolism was increased under anaerobic conditions (AN). Based on the metabolite profiles, L. plantarum strains were clustered into three main groups (A, B, and C). Overall, 79 and 83 significantly discriminant metabolites were characterized as chemical markers of AE and AN growth conditions, respectively. Notably, alcohols were more abundant in group A whereas amino acids, peptides, purines, and pyrimidines were significantly higher in group C. 2-hydroxy acids and oxylipins biosynthesized through amino acid and fatty acid metabolism, respectively, were more abundant in groups A and B. Furthermore, we observed a strong correlation between the chemical diversity of L. plantarum groups and their antioxidant activity from metabolite extracts. We propose a non-targeted metabolomic workflow to comprehensively characterize the chemodiversity of L. plantarum strain under different culture conditions, which may help reveal specific biomarkers of individual strains depending on the culture conditions.
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Previous reports indicate that L. plantarum strains exhibit distinct antioxidant activities and therapeutic effects even under identical conditions, underscoring the necessity of strain-level selection.
“This aligns with previous reports that L. plantarum strains exhibit distinct antioxidant activities and therapeutic effects even under identical conditions [25, 26], underscoring the necessity of strain-level selection.”
From Anti-Photoaging and Anti-Melanogenic Effects of a Novel <i>Lactiplantibacillus plantarum</i> RKBP Isolated from Giant <i>Centella asiatica</i> (2026) · cites this paper as [25]
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- Authors
- Kim, S.-H., Singh, D., Kim, S.-A., Kwak, M. J., Cho, D., Kim, J., Roh, J.-H., Kim, W.-G., Han, N. S., & Lee, C. H.
- Affiliations
- Chungbuk National University (KR)
- Konkuk University (KR)
- Amorepacific (South Korea) (KR)
- Corresponding author
- Lee, Choong Hwan
- Venue
- Food Microbiology
- Publisher
- Elsevier BV
- Published
- 2023-12
- Volume
- 116
- Pages
- 104364
- ISSN
- 0740-0020 (ISSN-L)
- OpenAlex
- W4385784019
- Cited by
- 12
- References
- 53
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 3
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- Food Microbiology (publishedVersion)
- SSRN Electronic Journal (acceptedVersion)
- PubMed (publishedVersion)
- Funders
- Konkuk University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Keywords
- Lactobacillus plantarum, oxylipins, amino acid metabolism, fatty acid metabolism, sugar metabolism, antioxidant activity, lactic acid bacteria
- MeSH terms
- Amino Acids, Anaerobiosis, Chromatography, Liquid, Fatty Acids, Hydroxy Acids, Tandem Mass Spectrometry
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