Cited by 1,008Metabolomics and Mass Spectrometry StudiesBioinformatics and Genomic NetworksMicrobial Metabolic Engineering and Bioproduction

Identification of bioactive metabolites using activity metabolomics

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Rinschen, Markus M., Ivanisevic, Julijana, Giera, Martin, Siuzdak, Gary·2019·Nature Reviews Molecular Cell Biology

Abstract

The metabolome, the collection of small-molecule chemical entities involved in metabolism, has traditionally been studied with the aim of identifying biomarkers in the diagnosis and prediction of disease. However, the value of metabolome analysis (metabolomics) has been redefined from a simple biomarker identification tool to a technology for the discovery of active drivers of biological processes. It is now clear that the metabolome affects cellular physiology through modulation of other ‘omics’ levels, including the genome, epigenome, transcriptome and proteome. In this Review, we focus on recent progress in using metabolomics to understand how the metabolome influences other omics and, by extension, to reveal the active role of metabolites in physiology and disease. This concept of utilizing metabolomics to perform activity screens to identify biologically active metabolites — which we term activity metabolomics — is already having a broad impact on biology. Metabolites can actively regulate biological processes and may directly modulate phenotype. The current challenge of metabolomics is to provide a platform for the discovery of such bioactive metabolites and — in combination with other omics technologies — to determine their biological functions.

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  1. [91]Branco Dos Santos F Probing the genome-scale metabolic landscape of Bordetella pertussis, the causative agent of whooping cough. Appl. Environ. Microbiol (2017). doi: 10.1128/AEM.01528-17 PMC5648915 28842544 · DOI

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Authors
Rinschen, M. M., Ivanisevic, J., Giera, M., & Siuzdak, G.
Affiliations
  • Scripps Research Institute (US)
  • Leiden University Medical Center (NL)
Corresponding author
  • Giera, Martin
  • Siuzdak, Gary
Venue
Nature Reviews Molecular Cell Biology
Published
2019-02-27
Volume
20
Issue
6
Pages
353-367
ISSN
  • 1471-0072 (ISSN-L)
  • 1471-0080
OpenAlex
W2916376502
Cited by
1,008
References
165
Citations by year
2026: 71 · 2025: 160 · 2024: 179 · 2023: 211 · 2022: 190 · 2021: 95 · 2020: 77 · 2019: 23 · 2018: 1 · 2015: 1
FWCI
8.72
Type
journal article
Language
en
Open access
Locations
  • Nature Reviews Molecular Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion, other-oa)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • Leiden Repository (Leiden University) (submittedVersion)
  • IRIS (submittedVersion)
  • SERVAL (Université de Lausanne) (submittedVersion)
Funders
  • National Institute of Mental Health (P30MH062261)
  • National Institute on Drug Abuse (P01DA028555)
  • National Cancer Institute (U01CA235493)
  • National Institute of General Medical Sciences (R01GM114368, R35GM130385)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
bioactive metabolites, metabolomics, metabolome, multi-omics integration, disease mechanisms, biomarkers
MeSH terms
Animals, Humans, Biomarkers, Metabolomics, Metabolome

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