Cited by 118Phytochemicals and Antioxidant ActivitiesAgricultural and Biological ResearchBotanical Research and Applications

Optimization and comparison of heat and ultrasound assisted extraction techniques to obtain anthocyanin compounds from Arbutus unedo L. Fruits

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López, Cecilia Jiménez, Caleja, Cristina, Prieto, M.A., Barreiro, Maria Filomena, Barros, Lillian, Ferreira, Isabel C.F.R.·2018·Food Chemistry

Abstract

The goal of this study was to compare anthocyanin extraction from Arbutus unedo L. fruits, by using two techniques assisted by different energy sources, heat and ultrasound. In order to obtain the conditions that maximize anthocyanin extraction, a response surface methodology was applied using the circumscribed central composite design of three variables with five levels. Three anthocyanin compounds were identified by HPLC-DAD-ESI/MS, being cyanidin-3-glucoside the main molecule. Heat assisted extraction proved to be the most effective method at 5 min, 90 °C and 80% of ethanol, yielding 51.2% of extract, with a total anthocyanin content of 382.4 µg/g dried fruit, and 744.6 µg/g extract. These response values were slightly improved by studying the solid/liquid effect at the optimal conditions in dose-response format, showing steady extraction values from 5 to 40 g/L. Results show the possibility of using A. unedo fruits as a source of anthocyanin compounds for industrial applications.

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Crossref · 10.1016/j.foodchem.2018.04.103

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  1. Clifford, M. N. (2000). Anthocyanins -nature, occurrence and dietary burden. Journal of the Science of Food and Agriculture, 80(7), 1063-1072.

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Authors
López, C. J., Caleja, C., Prieto, M. A., Barreiro, M. F., Barros, L., & Ferreira, I. C. F. R.
Affiliations
Universidade Politécnica de Bragança (PT)
Corresponding author
  • Barros, Lillian
  • Ferreira, Isabel C.F.R.
Venue
Food Chemistry
Publisher
Elsevier BV
Published
2018-10
Volume
264
Pages
81-91
ISSN
0308-8146 (ISSN-L)
OpenAlex
W2801625871
Cited by
118
References
48
Citations by year
2026: 2 · 2025: 7 · 2024: 8 · 2023: 18 · 2022: 15 · 2021: 16 · 2020: 25 · 2019: 21 · 2018: 6
FWCI
11.64
Type
journal article
Language
en
Open access
Locations
  • Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
  • Investigo Institutional repository of UVigo (Universidade de Vigo) (submittedVersion)
Funders
  • FCT
  • CIMO (UID/AGR/00690/2013)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Botanical Research and Applications
Keywords
anthocyanins, Arbutus unedo, heat-assisted extraction, ultrasound-assisted extraction, cyanidin-3-glucoside, extraction optimization, ethanol concentration, solid-to-liquid ratio, natural colorants
MeSH terms
Anthocyanins, Chromatography, High Pressure Liquid, Chemical Fractionation, Fruit, Glucosides, Hot Temperature, Ultrasonics, Spectrometry, Mass, Electrospray Ionization, Ericaceae

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