Optimization of enzyme assisted extraction of protein from the sugar beet (Beta vulgaris L.) leaves for alternative plant protein concentrate production
Abstract
This research focused on the effect of temperature (25-75 °C), extraction time (40-120 min) and solvent/solid ratio (15-45 mL/g) and the enzyme assisted extraction on protein extraction efficiency from by-product of sugar beet. 3 different methods were applied in protein isolation and the highest protein yield (34.55%) was obtained by the isoelectric-ammonium sulfate precipitation method. At the optimized condition of temperature 54.25 °C, 81.35 min, and solvent/solid ratio of 27.65 mL/g, the protein yield was found 55.15%. The protein yield has reached 79.01% with an increase of 43.27% with the aid of the enzyme assisted extraction. The physicochemical properties were determined for revealing its potential use in food industry. It is promising that the isolated protein concentrates (SPC-IAP) show high protein content (69.08% d.b) as well as high solubility (98.71% at pH 7.5). SPC-IAP's high brightness (L* = 79.55), low redness (a* = 0.33) and low yellowness (b* = 13.27) values are encouraging for food industry.
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Protein yield of 55.15% was obtained by isoelectric-ammonium sulfate precipitation method from sugar beet byproduct.
“Protein yield of 55.15% was obtained by isoelectric-ammonium sulfate precipitation method from sugar beet byproduct [117].”
From Food Waste Biorefinery: Pathway towards Circular Bioeconomy (2021) · cites this paper as [117]
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- Authors
- Akyüz, A., & Ersus, S.
- Affiliations
- Ege University (TR)
- Corresponding author
- Akyüz, Ayça
- Venue
- Food Chemistry
- Publisher
- Elsevier BV
- Published
- 2021-01
- Volume
- 335
- Pages
- 127673
- ISSN
- 0308-8146 (ISSN-L)
- OpenAlex
- W3046128027
- Cited by
- 183
- References
- 36
- Citations by year
- 2026: 23 · 2025: 48 · 2024: 52 · 2023: 22 · 2022: 28 · 2021: 10
- FWCI
- 9.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ege Üniversitesi
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Keywords
- enzymatic extraction, protein extraction, sugar beet leaves, Beta vulgaris, plant protein concentrates, sugar beet by-products, isoelectric precipitation, ammonium sulfate precipitation, extraction optimization, protein yield, protein solubility, physicochemical properties, color features, alternative plant protein
- MeSH terms
- Enzymes, Food-Processing Industry, Chemical Fractionation, Hydrogen-Ion Concentration, Plant Proteins, Powders, Solubility, Solvents, Temperature, Plant Leaves, Beta vulgaris
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