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Cited by 263Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchTransplantation: Methods and Outcomes

The DOI matches its Crossref record; 40 of the 41 references it cites were found in a registry or an index.

Perfusion decellularization of human and porcine lungs: Bringing the matrix to clinical scale

Gilpin, Sarah Elizabeth, Guyette, Jacques P., Gonzalez, Gabriel, Ren, Xi, Asara, John M., Mathisen, Douglas J., Vacanti, Joseph P., Ott, Harald C.·2014·The Journal of Heart and Lung Transplantation
DOI 10.1016/j.healun.2013.10.030
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Abstract

Background Organ engineering is a theoretical alternative to allotransplantation for end-stage organ failure. Whole-organ scaffolds can be created by detergent perfusion via the native vasculature, generating an acellular matrix suitable for recellularization with selected cell types. We aimed to up-scale this process, generating biocompatible scaffolds of a clinically relevant scale.Methods Rat, porcine, and human lungs were decellularized by detergent perfusion at constant pressures. Collagen, elastin, and glycosaminoglycan content of scaffolds were quantified by colorimetric assays. Proteomic analysis was performed by microcapillary liquid chromatography tandem mass spectrometry. Extracellular matrix (ECM) slices were cultured with human umbilical vein endothelial cells (HUVEC), small airway epithelial cells (SAEC), or pulmonary alveolar epithelial cells (PAECs) and evaluated by time-lapse live cell microscopy and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Whole-organ culture was maintained under constant-pressure media perfusion after seeding with PAECs.Results Rat lungs were decellularized using: (1) sodium dodecyl sulfate (SDS), (2) sodium deoxycholate (SDC), or (3) 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). Resulting scaffolds showed comparable loss of DNA but greatest preservation of ECM components in SDS-decellularized lungs. Porcine (n = 10) and human (n = 7) lungs required increased SDS concentration, perfusion pressures, and time to achieve decellularization as determined by loss of DNA, with preservation of intact matrix composition and lung architecture. Proteomic analysis of human decellularized lungs further confirmed ECM preservation. Recellularization experiments confirmed scaffold biocompatibility when cultured with mature cell phenotypes and scaffold integrity for the duration of biomimetic culture.Conclusions SDS-based perfusion decellularization can be applied to whole porcine and human lungs to generate biocompatible organ scaffolds with preserved ECM composition and architecture.

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Crossref · 10.1016/j.healun.2013.10.030

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Metadata

Authors
Gilpin, S. E., Guyette, J. P., Gonzalez, G., Ren, X., Asara, J. M., Mathisen, D. J., Vacanti, J. P., & Ott, H. C.
Affiliations
  • Harvard University (US)
  • Massachusetts General Hospital (US)
Corresponding author
Ott, Harald C.
ORCID
Venue
The Journal of Heart and Lung Transplantation
Publisher
Elsevier BV
Published
2014-03
Volume
33
Issue
3
Pages
298-308
ISSN
1053-2498 (ISSN-L)
DOI
10.1016/j.healun.2013.10.030
OpenAlex
W2047611832
Semantic Scholar
253777fdc37ab99c976b2319df807c9150ac0b39
Cited by
263
References
41
Citations by year
2026: 10 · 2025: 6 · 2024: 12 · 2023: 18 · 2022: 17 · 2021: 33 · 2020: 25 · 2019: 24 · 2018: 29 · 2017: 26 · 2016: 21 · 2015: 27 · 2014: 14
FWCI
20.37
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • The Journal of Heart and Lung Transplantation (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health (1dp2od008749-01)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Surgery › Transplantation: Methods and Outcomes
Keywords
perfusion decellularization, lung scaffolds, extracellular matrix preservation, sodium dodecyl sulfate, sodium deoxycholate, CHAPS, human lung, porcine lung, recellularization, alveolar epithelial cells, endothelial cells, small airway epithelial cells, whole organ culture, organ engineering, scaffold biocompatibility
MeSH terms
Animals, Biocompatible Materials, Cells, Cultured, Cholic Acids, Deoxycholic Acid, Detergents, Dose-Response Relationship, Drug, Endothelium, Vascular, Epithelial Cells, Extracellular Matrix, Humans, Lung, Perfusion, Sodium Dodecyl Sulfate, Swine, Rats, Sprague-Dawley, Models, Animal, Rats, Tissue Scaffolds, Bioengineering

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