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Functional Analysis via Standardized Whole-Blood Stimulation Systems Defines the Boundaries of a Healthy Immune Response to Complex Stimuli
Open accessAbstract
Standardization of immunophenotyping procedures has become a high priority. We have developed a suite of whole-blood, syringe-based assay systems that can be used to reproducibly assess induced innate or adaptive immune responses. By eliminating preanalytical errors associated with immune monitoring, we have defined the protein signatures induced by (1) medically relevant bacteria, fungi, and viruses; (2) agonists specific for defined host sensors; (3) clinically employed cytokines; and (4) activators of T cell immunity. Our results provide an initial assessment of healthy donor reference values for induced cytokines and chemokines and we report the failure to release interleukin-1α as a common immunological phenotype. The observed naturally occurring variation of the immune response may help to explain differential susceptibility to disease or response to therapeutic intervention. The implementation of a general solution for assessment of functional immune responses will help support harmonization of clinical studies and data sharing.
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“TruCulture® reproducibly reveals the induced innate and adaptive immune response in whole blood after stimulation, by quantifying the release of soluble immune activation products (cytokines, chemokines, soluble receptors etc.) in the supernatant and by measuring the transcription level (mRNA) in the circulating blood (immune) cells [77].”
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Metadata
- Authors
- Duffy, D., Rouilly, V., Libri, V., Hasan, M., Beitz, B., David, M., Urrutia, A., Bisiaux, A., LaBrie, S. T., Dubois, A., Boneca, I. G., Delval, C., Thomas, S., Rogge, L., Schmolz, M., Quintana-Murci, L., Albert, M. L., Abel, L., Alcover, A., Bousso, P., Cumano, A., Daëron, M., Delval, C., Demangel, C., Deriano, L., Di Santo, J., Dromer, F., Eberl, G., Enninga, J., Freitas, A., Gomperts-Boneca, I., Hercberg, S., Lantz, O., Leclerc, C., Mouquet, H., Pellegrini, S., Pol, S., Rogge, L., Sakuntabhai, A., Schwartz, O., Schwikowski, B., Shorte, S., Soumelis, V., Tangy, F., Tartour, E., Toubert, A., Ungeheuer, M.-N., Quintana-Murci, L., & Albert, M. L.
- Affiliations
- Inserm (FR)
- Institut Pasteur (FR)
- Myriad Genetics (US)
- Centre d’Immunologie de Marseille-Luminy (FR)
- Department of Genomes & Genetics (FR)
- Hôpital Cochin (FR)
- Centre de Recherche sur les Liens Sociaux (FR)
- Hôpital Saint-Louis (FR)
- ORCID
- Duffy, Darragh
- Rouilly, Vincent
- Libri, Valentina
- Hasan, Milena
- Urrutia, Alejandra
- Bisiaux, Aurélie
- Schmolz, Manfred
- Abel, Laurent
- Bousso, Philippe
- Cumano, Ana
- Daëron, Marc
- Demangel, Caroline
- Deriano, Ludovic
- Dromer, Françoise
- Eberl, Gérard
- Enninga, Jost
- Lantz, Olivier
- Leclerc, Claude
- Mouquet, Hugo
- Pellegrini, Sandra
- Pol, Stanislas
- Sakuntabhai, Anavaj
- Schwartz, Olivier
- Schwikowski, Benno
- Shorte, Spencer
- Soumelis, Vassili
- Tangy, Frédéric
- Toubert, Antoine
- Ungeheuer, Marie-Noëlle
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2014-03
- Volume
- 40
- Issue
- 3
- Pages
- 436-450
- ISSN
- 1074-7613 (ISSN-L)
- OpenAlex
- W1984830992
- Cited by
- 242
- References
- 85
- Citations by year
- 2026: 16 · 2025: 14 · 2024: 23 · 2023: 17 · 2022: 34 · 2021: 23 · 2020: 21 · 2019: 18 · 2018: 18 · 2017: 19 · 2016: 13 · 2015: 20 · 2014: 6
- FWCI
- 8.83
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Immunity (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Keywords
- innate immune response, adaptive immune response, cytokine signature, chemokines, interleukin-1 alpha, pre-analytical errors, T cell activation, immune response variability
- MeSH terms
- Antigens, Humans, Immunity, Innate, Reference Values, Monitoring, Immunologic, Reproducibility of Results, Cytokines, Inflammation Mediators, Adaptive Immunity, Healthy Volunteers
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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