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Human dermal CD14(+) cells are a transient population of monocyte-derived macrophages
Open accessAbstract
Dendritic cells (DCs), monocytes, and macrophages are leukocytes with critical roles in immunity and tolerance. The DC network is evolutionarily conserved; the homologs of human tissue CD141(hi)XCR1⁺ CLEC9A⁺ DCs and CD1c⁺ DCs are murine CD103⁺ DCs and CD64⁻ CD11b⁺ DCs. In addition, human tissues also contain CD14⁺ cells, currently designated as DCs, with an as-yet unknown murine counterpart. Here we have demonstrated that human dermal CD14⁺ cells are a tissue-resident population of monocyte-derived macrophages with a short half-life of <6 days. The decline and reconstitution kinetics of human blood CD14⁺ monocytes and dermal CD14⁺ cells in vivo supported their precursor-progeny relationship. The murine homologs of human dermal CD14⁺ cells are CD11b⁺ CD64⁺ monocyte-derived macrophages. Human and mouse monocytes and macrophages were defined by highly conserved gene transcripts, which were distinct from DCs. The demonstration of monocyte-derived macrophages in the steady state in human tissue supports a conserved organization of human and mouse mononuclear phagocyte system.
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Metadata
- Authors
- McGovern, N., Schlitzer, A., Gunawan, M., Jardine, L., Shin, A., Poyner, E., Green, K., Dickinson, R., Wang, X.-N., Low, D., Best, K., Covins, S., Milne, P., Pagan, S., Aljefri, K., Windebank, M., Miranda-Saavedra, D., Larbi, A., Wasan, P. S., Duan, K., Poidinger, M., Bigley, V., Ginhoux, F., Collin, M., & Haniffa, M.
- Affiliations
- Agency for Science, Technology and Research (SG)
- Newcastle University (GB)
- Corresponding author
- Collin, Matthew
- Haniffa, Muzlifah
- ORCID
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2014-09
- Volume
- 41
- Issue
- 3
- Pages
- 465-477
- ISSN
- 1074-7613 (ISSN-L)
- PMC
- PMC4175180
- OpenAlex
- W2144829409
- Cited by
- 281
- References
- 54
- Citations by year
- 2026: 3 · 2025: 12 · 2024: 13 · 2023: 17 · 2022: 11 · 2021: 24 · 2020: 34 · 2019: 32 · 2018: 25 · 2017: 37 · 2016: 31 · 2015: 36 · 2014: 6
- FWCI
- 16.76
- Type
- journal article
- Language
- en
- Update notices
- erratum (2015-02-17)
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Immunity (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Apollo (University of Cambridge) (submittedVersion, cc-by)
- Apollo (cc-by)
- Funders
- Histiocytosis Association
- Wellcome Trust (101155/Z/13/Z, WT088555MA)
- British Skin Foundation
- Leukaemia and Lymphoma Research
- Newcastle University
- AXA Research Fund
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- CD14+ cells, monocyte-derived macrophages, dermal macrophages, monocytes, monocyte-to-macrophage differentiation, tissue-resident macrophages, reticuloendothelial system, CD11b, CD64, dendritic cells, CD1c+ dendritic cells, CD103+ dendritic cells, human-mouse comparison, macrophage turnover
- MeSH terms
- Animals, Cell Differentiation, Cell Movement, Cells, Cultured, Dendritic Cells, Female, Humans, Immunologic Memory, Macrophages, Mice, Transgenic, Skin, T-Lymphocytes, Receptors, IgG, Lipopolysaccharide Receptors, Cell Lineage, CD11b Antigen, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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