Cited by 23T-cell and B-cell ImmunologyGenomics and Chromatin DynamicsPsoriasis: Treatment and Pathogenesis

Neuroprotective protein ADNP-dependent histone remodeling complex promotes T helper 2 immune cell differentiation

Open access
Ferreira, Ana C.F., Szeto, Aydan C.H., Clark, Paula A., Crisp, Alastair, Kozik, Patrycja, Jolin, Helen E., McKenzie, Andrew N.J.·2023·Immunity

Abstract

Type 2 immune responses are critical in tissue homeostasis, anti-helminth immunity, and allergy. T helper 2 (Th2) cells produce interleukin-4 (IL-4), IL-5, and IL-13 from the type 2 gene cluster under regulation by transcription factors (TFs) including GATA3. To better understand transcriptional regulation of Th2 cell differentiation, we performed CRISPR-Cas9 screens targeting 1,131 TFs. We discovered that activity-dependent neuroprotector homeobox protein (ADNP) was indispensable for immune reactions to allergen. Mechanistically, ADNP performed a previously unappreciated role in gene activation, forming a critical bridge in the transition from pioneer TFs to chromatin remodeling by recruiting the helicase CHD4 and ATPase BRG1. Although GATA3 and AP-1 bound the type 2 cytokine locus in the absence of ADNP, they were unable to initiate histone acetylation or DNA accessibility, resulting in highly impaired type 2 cytokine expression. Our results demonstrate an important role for ADNP in promoting immune cell specialization.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.immuni.2023.05.010

Cites

73 of 73 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Neuroprotective agents such as NAP, the activity-dependent neuroprotective protein generated from ADNP, have been shown to be useful in preventing the death of neurons, in order to preserve microtubules and maintain neuronal function.

    “In order to preserve microtubules and maintain neuronal function, neuroprotective agents—substances such as NAP, the activity-dependent neuroprotective protein generated from ADNP—have been shown to be useful in preventing the death of neurons [211,212].”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Ferreira, A. C. F., Szeto, A. C. H., Clark, P. A., Crisp, A., Kozik, P., Jolin, H. E., & McKenzie, A. N. J.
Affiliations
MRC Laboratory of Molecular Biology (GB)
Corresponding author
  • Ferreira, Ana C.F.
  • McKenzie, Andrew N.J.
Venue
Immunity
Publisher
Elsevier BV
Published
2023-07
Volume
56
Issue
7
Pages
1468-1484.e7
ISSN
1074-7613 (ISSN-L)
OpenAlex
W4379537564
Cited by
23
References
73
Citations by year
2026: 3 · 2025: 8 · 2024: 10 · 2023: 2
FWCI
2.52
Type
journal article
Language
en
Open access
Locations
  • Immunity (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion, cc-by)
Funders
Wellcome Trust (100963/Z/13/Z, 220223/Z/20/Z)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
Keywords
ADNP, Th2 immune response, CHD4, SMARCA4, GATA3, AP-1, histone acetylation, DNA accessibility, pioneer transcription factors, CRISPR-Cas9 screen, transcriptional regulation
MeSH terms
Cell Differentiation, Gene Expression Regulation, Histones, Transcription Factors, Cytokines, Th2 Cells, GATA3 Transcription Factor

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review