ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors
Open accessAbstract
Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.
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ChIPmentation experiments were performed as previously described with few modifications.
“ChIPmentation experiments were performed as previously described,76 with few modifications.”
From Super-enhancers include classical enhancers and facilitators to fully activate gene expression (2023) · cites this paper as [76]
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- Authors
- Schmidl, C., Rendeiro, A. F., Sheffield, N. C., & Bock, C.
- Affiliations
- Austrian Academy of Sciences (AT)
- Corresponding author
- Bock, Christoph
- Venue
- Nature Methods
- Published
- 2015-08-17
- Volume
- 12
- Issue
- 10
- Pages
- 963-965
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC4589892
- OpenAlex
- W1925000049
- Cited by
- 509
- References
- 1
- Citations by year
- 2026: 44 · 2025: 35 · 2024: 60 · 2023: 41 · 2022: 43 · 2021: 60 · 2020: 67 · 2019: 63 · 2018: 44 · 2017: 26 · 2016: 23 · 2015: 2 · 2014: 1
- FWCI
- 13.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Figshare (submittedVersion, cc-by)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Figshare (cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- tagmentation, Tn5 transposase, chromatin immunoprecipitation, histone modifications, transcription factor binding, low-input sequencing
- MeSH terms
- Histones, Humans, Transcription Factors, Genome, Human, K562 Cells, Chromatin Immunoprecipitation
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